| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35401-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-03535-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59933-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-97936-50ul | |||
![Immunohistochemistry: HUS1 Antibody [NBP3-35401] - Immunohistochemistry analysis of paraffin-embedded Rat heart using HUS1 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35401_rabbit-hus1-pab-91220241519590.jpg "Immunohistochemistry: HUS1 Antibody [NBP3-35401] - Immunohistochemistry analysis of paraffin-embedded Rat heart using HUS1 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.")
![Immunohistochemistry: HUS1 Antibody [NBP3-35401] - Immunohistochemistry analysis of paraffin-embedded Human gastric cancer using HUS1 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35401_rabbit-hus1-pab-91220241513346.jpg)
![Immunohistochemistry: HUS1 Antibody [NBP3-35401] - Immunohistochemistry analysis of paraffin-embedded Rat liver using HUS1 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35401_rabbit-hus1-pab-91220241418155.jpg)
![Western Blot: HUS1 Antibody [NBP3-35401] - Western blot analysis of lysates from mouse kidney, using HUS1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.](http://images.novusbio.com/fullsize/nbp3-35401_rabbit-hus1-pab-912202414213762.jpg)
![Immunohistochemistry: HUS1 Antibody [NBP3-35401] - Immunohistochemistry analysis of paraffin-embedded Human uterine cancer using HUS1 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35401_rabbit-hus1-pab-912202414163053.jpg)
![Immunocytochemistry/ Immunofluorescence: PIDD Antibody - BSA Free [PIDD] - Immunofluorescence analysis of HeLa cells using PIDD Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-03535_rabbit-polyclonal-lrdd-antibody-52202516334114.jpg "Immunocytochemistry/ Immunofluorescence: PIDD Antibody - BSA Free [PIDD] - Immunofluorescence analysis of HeLa cells using PIDD Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Immunohistochemistry-Paraffin: LRDD Antibody [NBP3-03535] - Paraffin-embedded mouse brain using LRDD antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/LRDD-Antibody-Immunohistochemistry-Paraffin-NBP3-03535-img0004.jpg)
![Immunohistochemistry-Paraffin: LRDD Antibody [NBP3-03535] - Paraffin-embedded human lung cancer using LRDD antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/LRDD-Antibody-Immunohistochemistry-Paraffin-NBP3-03535-img0002.jpg)
![Immunohistochemistry-Paraffin: LRDD Antibody [NBP3-03535] - Paraffin-embedded rat brain using LRDD antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/LRDD-Antibody-Immunohistochemistry-Paraffin-NBP3-03535-img0003.jpg)
![Western Blot: lin-54 Antibody [NBP2-82002] - Analysis of lin-54 in HeLa cell lysate with lin-54 antibody at 1 ug/ml.](http://images.novusbio.com/fullsize/lin-54-Antibody-Western-Blot-NBP2-82002-img0001.jpg "Western Blot: lin-54 Antibody [NBP2-82002] - Analysis of lin-54 in HeLa cell lysate with lin-54 antibody at 1 ug/ml.")
![Immunocytochemistry/Immunofluorescence: lin-54 Antibody [NBP2-82002] - Analysis of lin-54 in HeLa cells with lin-54 antibody at 20 ug/ml. Red: lin-54 antibody [NBP2-82002]. Blue: DAPI staining.](http://images.novusbio.com/fullsize/lin-54-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-82002-img0005.jpg)
![Immunocytochemistry/ Immunofluorescence: lin-54 Antibody - BSA Free [NBP2-82002] - Immunofluorescence of lin-54 in HeLa cells with lin-54 antibody at 20 u/ml.](http://images.novusbio.com/fullsize/nbp2-82002_rabbit-polyclonal-lin-54-antibody-2711202419241351.jpg)
![Immunocytochemistry/Immunofluorescence: lin-54 Antibody [NBP2-82002] - Analysis of lin-54 in HeLa cells with lin-54 antibody at 10 ug/ml.](http://images.novusbio.com/fullsize/lin-54-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-82002-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: lin-54 Antibody - BSA Free [NBP2-82002] - Immunocytochemistry of lin-54 in HeLa cells with lin-54 at 2.5 u/mL.](http://images.novusbio.com/fullsize/nbp2-82002_rabbit-polyclonal-lin-54-antibody-2711202420212363.gif)
![Western Blot: Cyclin B2 Antibody (1L9B9) [NBP3-16663] - Western blot analysis of extracts of Rat testis cells, using Cyclin B2 antibody (NBP3-16663) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/Cyclin-B2-Antibody-1L9B9-Western-Blot-NBP3-16663-img0003.jpg "Western Blot: Cyclin B2 Antibody (1L9B9) [NBP3-16663] - Western blot analysis of extracts of Rat testis cells, using Cyclin B2 antibody (NBP3-16663) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Immunocytochemistry/Immunofluorescence: Cyclin B2 Antibody (1L9B9) [NBP3-16663] - Immunofluorescence analysis of NIH-3T3 cells using Cyclin B2 Rabbit mAb (NBP3-16663) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Cyclin-B2-Antibody-1L9B9-Immunocytochemistry-Immunofluorescence-NBP3-16663-img0002.jpg)
![Immunohistochemistry-Paraffin: Cyclin B2 Antibody (1L9B9) [NBP3-16663] - Immunohistochemistry of paraffin-embedded human liver cancer using Cyclin B2 Rabbit mAb (NBP3-16663) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Cyclin-B2-Antibody-1L9B9-Immunohistochemistry-Paraffin-NBP3-16663-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: Cyclin B2 Antibody (1L9B9) [NBP3-16663] - Confocal imaging of NIH/3T3 cells using Cyclin B2 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo(R) 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16663_rabbit-cyclin-b2-mab-1l9b9-52202511392244.jpg)
![Western Blot: MCM5 Antibody (4P1Q5) [NBP3-16529] - Western blot analysis of extracts of various cell lines, using MCM5 Rabbit mAb (NBP3-16529) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.](http://images.novusbio.com/fullsize/MCM5-Antibody-4P1Q5-Western-Blot-NBP3-16529-img0003.jpg "Western Blot: MCM5 Antibody (4P1Q5) [NBP3-16529] - Western blot analysis of extracts of various cell lines, using MCM5 Rabbit mAb (NBP3-16529) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.")
![Immunohistochemistry-Paraffin: MCM5 Antibody (4P1Q5) [NBP3-16529] - Immunohistochemistry of paraffin-embedded human colon using MCM5 Rabbit mAb (NBP3-16529) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/MCM5-Antibody-4P1Q5-Immunohistochemistry-Paraffin-NBP3-16529-img0002.jpg)
![Immunohistochemistry-Paraffin: MCM5 Antibody (4P1Q5) [NBP3-16529] - Immunohistochemistry of paraffin-embedded rat testis using MCM5 Rabbit mAb (NBP3-16529) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/MCM5-Antibody-4P1Q5-Immunohistochemistry-Paraffin-NBP3-16529-img0001.jpg)
![Immunoprecipitation: MCM5 Antibody (4P1Q5) [NBP3-16529] - Immunoprecipitation analysis of 300ug extracts of MCF7 cells using 3ug MCM5 antibody (NBP3-16529). Western blot was performed from the immunoprecipitate using MCM5 antibody (NBP3-16529) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/MCM5-Antibody-4P1Q5-Immunoprecipitation-NBP3-16529-img0004.jpg)
![Western Blot: Lamin A + C Antibody (WL4G10) [NBP2-59933] - Lamin A + C (WL4G10) [NBP2-59933] - Whole cell lysates from HeLa and 293T cells were separated on SDS-PAGE gel, transferred to nitrocellulose membrane and stained with WL4G10 antibody. The membrane was blocked overnight in blocking buffer (5% nonfat dry milk resuspended in 0.1% Tween and 1X PBS). Both primary and secondary antibodies were diluted in blocking buffer. As expected, 293T cells express less of the lamin A isoform.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-WL4G10-Western-Blot-NBP2-59933-img0006.jpg "Western Blot: Lamin A + C Antibody (WL4G10) [NBP2-59933] - Lamin A + C (WL4G10) [NBP2-59933] - Whole cell lysates from HeLa and 293T cells were separated on SDS-PAGE gel, transferred to nitrocellulose membrane and stained with WL4G10 antibody. The membrane was blocked overnight in blocking buffer (5% nonfat dry milk resuspended in 0.1% Tween and 1X PBS). Both primary and secondary antibodies were diluted in blocking buffer. As expected, 293T cells express less of the lamin A isoform.")
![Immunohistochemistry-Paraffin: Lamin A + C Antibody (WL4G10) [NBP2-59933] - Analysis of formalin-fixed paraffin-embedded human gut. Following heat induced antigen retrieval, the sections were stained with mouse monoclonal anti-lamin A+C clone WL4G10 antibody (green). Nuclei were visualized with DAPI.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-WL4G10-Immunocytochemistry-Immunofluorescence-NBP2-59933-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Lamin A + C Antibody (WL4G10) [NBP2-59933] - Confocal images of methanol fixed Hela cells that were immunofluorescently stained with mouse monoclonal WL4G10 antibody. The antibody works as well on cells fixed with 4% PFA.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-WL4G10-Immunocytochemistry-Immunofluorescence-NBP2-59933-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Lamin A + C Antibody (WL4G10) [NBP2-59933] - The mouse monoclonal WL4G10 antibody recognizes human lamins A and C. Immortalized mouse adult LMNA null fibroblast stably expressing: (A) mEos2-lamin A or (B) mEos2- lamin C fused were fixed in 4% PFA and stained with WL4G10 antibody (magenta). Expression of the respective LMNA isoform is shown in green. The nuclei were counter-stained with Hoechst. Of note, the WL4G10 antibody does not recognize mouse lamins A and C.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-WL4G10-Immunocytochemistry-Immunofluorescence-NBP2-59933-img0003.jpg)
![Western Blot: CDK4 Antibody (DCS-31) [NB120-6315] - WB analysis of lysates from human HeLa and SKUT cells using CDK4 antibody (clone DCS-31).](http://images.novusbio.com/fullsize/CDK4-Antibody-DCS-31-Western-Blot-NB120-6315-img0001.jpg "Western Blot: CDK4 Antibody (DCS-31) [NB120-6315] - WB analysis of lysates from human HeLa and SKUT cells using CDK4 antibody (clone DCS-31).")
![Western Blot: CDC25C Antibody (JE59-53) [NBP3-32173] - Western blot analysis of CDC25C on different lysates with Rabbit anti-CDC25C antibody (NBP3-32173) at 1/1,000 dilution. Lane 1: 293T cell lysate Lane 2: Caco-2 cell lysate Lane 3: HUVEC cell lysate Lane 4: HT-29 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 53 kDa Observed band size: 58 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32173) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32173_rabbit-cdc25c-mab-je59-53-27820241631628.jpg "Western Blot: CDC25C Antibody (JE59-53) [NBP3-32173] - Western blot analysis of CDC25C on different lysates with Rabbit anti-CDC25C antibody (NBP3-32173) at 1/1,000 dilution. Lane 1: 293T cell lysate Lane 2: Caco-2 cell lysate Lane 3: HUVEC cell lysate Lane 4: HT-29 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 53 kDa Observed band size: 58 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32173) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: CDC25C Antibody (JE59-53) [NBP3-32173] - Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-CDC25C antibody (NBP3-32173) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32173) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32173_rabbit-cdc25c-mab-je59-53-29820241420958.jpg)
![Immunocytochemistry/ Immunofluorescence: CDC25C Antibody (JE59-53) [NBP3-32173] - Immunocytochemistry analysis of HeLa cells labeling CDC25C with Rabbit anti-CDC25C antibody (NBP3-32173) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CDC25C antibody (NBP3-32173) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32173_rabbit-cdc25c-mab-je59-53-2920241023428.jpg)
![Flow Cytometry: CDC25C Antibody (JE59-53) [NBP3-32173] - Flow cytometric analysis of HeLa cells labeling CDC25C. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32173, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32173_rabbit-cdc25c-mab-je59-53-29202416453230.jpg)
![Western Blot: p21/CIP1/CDKN1A Antibody (PSH03-93) [NBP3-32691] - Western blot analysis of p21/CIP1/CDKN1A on different lysates with Rabbit anti-p21/CIP1/CDKN1A antibody (NBP3-32691) at 1/1,000 dilution. Lane 1: NIH/3T3 cell lysate Lane 2: RAW264.7 cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 18 kDa Observed band size: 21 kDa Exposure time: Lane 1: 1 minute; Lane 2: 9 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32691) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32691_rabbit-p21-cip1-cdkn1a-mab-psh03-93-278202416331255.jpg "Western Blot: p21/CIP1/CDKN1A Antibody (PSH03-93) [NBP3-32691] - Western blot analysis of p21/CIP1/CDKN1A on different lysates with Rabbit anti-p21/CIP1/CDKN1A antibody (NBP3-32691) at 1/1,000 dilution. Lane 1: NIH/3T3 cell lysate Lane 2: RAW264.7 cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 18 kDa Observed band size: 21 kDa Exposure time: Lane 1: 1 minute; Lane 2: 9 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32691) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: p21/CIP1/CDKN1A Antibody (PSH03-93) [NBP3-32691] - Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-p21/CIP1/CDKN1A antibody (NBP3-32691) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32691) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32691_rabbit-p21-cip1-cdkn1a-mab-psh03-93-29820241426265.jpg)
![Immunocytochemistry/ Immunofluorescence: p21/CIP1/CDKN1A Antibody (PSH03-93) [NBP3-32691] - Immunocytochemistry analysis of RAW264.7 cells labeling p21/CIP1/CDKN1A with Rabbit anti-p21/CIP1/CDKN1A/CIP1/CDKN1A antibody (NBP3-32691) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-p21/CIP1/CDKN1A antibody (NBP3-32691) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32691_rabbit-p21-cip1-cdkn1a-mab-psh03-93-29202410334083.jpg)
![Flow Cytometry: p21/CIP1/CDKN1A Antibody (PSH03-93) [NBP3-32691] - Flow cytometric analysis of RAW264.7 cells labeling p21/CIP1/CDKN1A. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32691, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32691_rabbit-p21-cip1-cdkn1a-mab-psh03-93-29202416372178.jpg)
![Western Blot: CDK2 [p Tyr15] Antibody (SN72-04) [NBP2-67686] - Lane 1: Hela cells, whole cell lysate, 10ug/lane Lane 2: Hela cells treated with 2.8ug/ul lambda-PP for 30 minutes, whole cell lysates, 10ug/lane All lanes : Anti-Phospho-Cdk2(Y15) antibody at 1/500 dilution. Anti-Cdk2 antibody at 1/500 dilution. Anti-GAPDH antibody at 1/10,000 dilution. Goat Anti-Rabbit IgG H&L (HRP) at 1/200,000 dilution. Predicted band size: 34 kDa Observed band size: 34 kDa Blocking and diluting buffer: 5% BSA. Exposure time: 30 seconds](http://images.novusbio.com/fullsize/CDK2-p-Tyr15-Antibody-SN72-04-Western-Blot-NBP2-67686-img0004.jpg "Western Blot: CDK2 [p Tyr15] Antibody (SN72-04) [NBP2-67686] - Lane 1: Hela cells, whole cell lysate, 10ug/lane Lane 2: Hela cells treated with 2.8ug/ul lambda-PP for 30 minutes, whole cell lysates, 10ug/lane All lanes : Anti-Phospho-Cdk2(Y15) antibody at 1/500 dilution. Anti-Cdk2 antibody at 1/500 dilution. Anti-GAPDH antibody at 1/10,000 dilution. Goat Anti-Rabbit IgG H&L (HRP) at 1/200,000 dilution. Predicted band size: 34 kDa Observed band size: 34 kDa Blocking and diluting buffer: 5% BSA. Exposure time: 30 seconds")
![Immunohistochemistry-Paraffin: CDK2 [p Tyr15] Antibody (SN72-04) [NBP2-67686] - Analysis of paraffin-embedded mouse colon tissue using anti-Phospho-Cdk2(Y15) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CDK2-p-Tyr15-Antibody-SN72-04-Immunohistochemistry-Paraffin-NBP2-67686-img0002.jpg)
![Western Blot: CDK2 [p Tyr15] Antibody (SN72-04) [NBP2-67686] - Lane 1: Hela cell lysate (10 ug/Lane)Lane 2: NIH/3T3 cell lysate (10 ug/Lane)Lane 3: Mouse spleen tissue lysate (20 ug/Lane)Predicted band size: 34 kDa Observed band size: 35 kDa Exposure time: 1 minute; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-52) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/CDK2-p-Tyr15-Antibody-SN72-04-Western-Blot-NBP2-67686-img0003.jpg)
![Immunohistochemistry-Paraffin: CDK2 [p Tyr15] Antibody (SN72-04) [NBP2-67686] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-Phospho-Cdk2(Y15) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CDK2-p-Tyr15-Antibody-SN72-04-Immunohistochemistry-Paraffin-NBP2-67686-img0001.jpg)
![Western Blot: MCM2 Antibody (PD00-90) [NBP3-32576] - Western blot analysis of MCM2 on different lysates with Rabbit anti-MCM2 antibody (NBP3-32576) at 1/2,000 dilution. Lane 1: HL-60 cell lysate Lane 2: SiHa cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 102 kDa Observed band size: 130 kDa Exposure time: 1 minute; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32576) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32576_rabbit-mcm2-mab-pd00-90-27820241631683.jpg "Western Blot: MCM2 Antibody (PD00-90) [NBP3-32576] - Western blot analysis of MCM2 on different lysates with Rabbit anti-MCM2 antibody (NBP3-32576) at 1/2,000 dilution. Lane 1: HL-60 cell lysate Lane 2: SiHa cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 102 kDa Observed band size: 130 kDa Exposure time: 1 minute; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32576) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: MCM2 Antibody (PD00-90) [NBP3-32576] - Immunohistochemical analysis of paraffin-embedded human cervical carcinoma tissue with Rabbit anti-MCM2 antibody (NBP3-32576) at 1/800 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32576) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32576_rabbit-mcm2-mab-pd00-90-298202414262619.jpg)
![Immunocytochemistry/ Immunofluorescence: MCM2 Antibody (PD00-90) [NBP3-32576] - Immunocytochemistry analysis of LOVO cells labeling MCM2 with Rabbit anti-MCM2 antibody (NBP3-32576) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-MCM2 antibody (NBP3-32576) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32576_rabbit-mcm2-mab-pd00-90-2920241114811.jpg)
![Flow Cytometry: MCM2 Antibody (PD00-90) [NBP3-32576] - Flow cytometric analysis of HL-60 cells labeling MCM2. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32576, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32576_rabbit-mcm2-mab-pd00-90-2920241637215.jpg)
![Western Blot: RFC4 Antibody [NBP2-97936] - Anti-RFC4 rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 40 kDa. Observed band size: 40 kDa](http://images.novusbio.com/fullsize/RFC4-Antibody-Western-Blot-NBP2-97936-img0005.jpg "Western Blot: RFC4 Antibody [NBP2-97936] - Anti-RFC4 rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 40 kDa. Observed band size: 40 kDa")
![Immunohistochemistry-Paraffin: RFC4 Antibody [NBP2-97936] - Immunochemical staining of human RFC4 in human small intestine with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/RFC4-Antibody-Immunohistochemistry-Paraffin-NBP2-97936-img0004.jpg)
![Immunoprecipitation: RFC4 Antibody [NBP2-97936] - RFC4 was immunoprecipitated using: Lane A: 0.5 mg Jurkat Whole Cell Lysate 4 ul anti-RFC4 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-RFC4 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 40 kDa. Observed band size: 40 kDa](http://images.novusbio.com/fullsize/RFC4-Antibody-Immunoprecipitation-NBP2-97936-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: RFC4 Antibody [NBP2-97936] - Immunofluorescence staining of RFC4 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-97936 antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.](http://images.novusbio.com/fullsize/nbp2-97936_rabbit-polyclonal-rfc4-antibody-23520251664787.png)