| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21294-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35368-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89498-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-90922-25ul | |||
![Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Hexokinase 2 antibody [HL2000] (NBP3-48667) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48667_rabbit-hexokinase-2-mab-hl2000-western-blot-1411202518324118.jpg "Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Hexokinase 2 antibody [HL2000] (NBP3-48667) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Non-transfected (-) and transfected (+) 293T whole cell extracts were separated by 5% SDS-PAGE, and the membrane was blotted with Hexokinase 2 antibody [HL2000] (NBP3-48667) diluted at 1:25000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48667_rabbit-hexokinase-2-mab-hl2000-western-blot-1411202518443413.jpg)
![Zebrafish tissue extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with Hexokinase 2 antibody [HL2000] (NBP3-48667) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48667_rabbit-hexokinase-2-mab-hl2000-western-blot-141120251840430.jpg)
![Whole Japanese medaka extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with Hexokinase 2 antibody [HL2000] (NBP3-48667) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-48667_rabbit-hexokinase-2-mab-hl2000-western-blot-141120251843314.jpg)
![Western Blot: VE-Cadherin Antibody (16B1) [NBP2-00342] - EDTA-passaged (left) and Trypsin-passaged (right) Human Umbilical Vein Endothelial Cells (HUVEC) were resolved by SDS -PAGE and immunoblotted with 2 ug/mL of Anti-Human CD144 (VE-Cadherin) Purified and revealed with Anti-Rat IgG HRP.](http://images.novusbio.com/fullsize/VE-Cadherin-Antibody-16B1-Western-Blot-NBP2-00342-img0002.jpg "Western Blot: VE-Cadherin Antibody (16B1) [NBP2-00342] - EDTA-passaged (left) and Trypsin-passaged (right) Human Umbilical Vein Endothelial Cells (HUVEC) were resolved by SDS -PAGE and immunoblotted with 2 ug/mL of Anti-Human CD144 (VE-Cadherin) Purified and revealed with Anti-Rat IgG HRP.")
![Flow Cytometry: VE-Cadherin Antibody (16B1) [NBP2-00342] - Analysis using the Biotin conjugate of NBP2-00342. Staining of Human Umbilical Vein Endothelial Cells (HUVEC) with 0.25 ug of Mouse IgG1 kappa Isotype Control Biotin (open histogram) or 0.125 ug of Anti-Human CD144 (VE-Cadherin) Biotin (filled histogram)](http://images.novusbio.com/fullsize/VE-Cadherin-Antibody-16B1-Flow-Cytometry-NBP2-00342-img0007.jpg)
![Flow Cytometry: VE-Cadherin Antibody (16B1) [NBP2-00342] - Analysis using the Allophycocyanin conjugate of NBP2-00342. Staining of Human Umbilical Vein Endothelial Cells.](http://images.novusbio.com/fullsize/VE-Cadherin-Antibody-16B1-Flow-Cytometry-NBP2-00342-img0006.jpg)
![Flow Cytometry: VE-Cadherin Antibody (16B1) [NBP2-00342] - Analysis using the PE conjugate of NBP2-00342.](http://images.novusbio.com/fullsize/VE-Cadherin-Antibody-16B1-Flow-Cytometry-NBP2-00342-img0008.jpg)
![Western Blot: VE-Cadherin Antibody (PSH03-15) [NBP3-33088] - Western blot analysis of VE-Cadherin on different lysates with Rabbit anti-VE-Cadherin antibody (NBP3-33088) at 1/2,000 dilution. Lane 1: EA.hy926-si NT cell lysate Lane 2: EA.hy926-si VE Cadherin cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 88 kDa Observed band size: 140 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33088) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33088_rabbit-ve-cadherin-mab-psh03-15-288202414191513.jpg "Western Blot: VE-Cadherin Antibody (PSH03-15) [NBP3-33088] - Western blot analysis of VE-Cadherin on different lysates with Rabbit anti-VE-Cadherin antibody (NBP3-33088) at 1/2,000 dilution. Lane 1: EA.hy926-si NT cell lysate Lane 2: EA.hy926-si VE Cadherin cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 88 kDa Observed band size: 140 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33088) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: VE-Cadherin Antibody (PSH03-15) [NBP3-33088] - Immunocytochemistry analysis of HeLa cells labeling VE-Cadherin with Rabbit anti-VE-Cadherin antibody (NBP3-33088) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-VE-Cadherin antibody (NBP3-33088) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-33088_rabbit-ve-cadherin-mab-psh03-15-29202410234272.jpg)
![Flow Cytometry: VE-Cadherin Antibody (PSH03-15) [NBP3-33088] - Flow cytometric analysis of EA.hy926 cells labeling VE-Cadherin. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (NBP3-33088, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-33088_rabbit-ve-cadherin-mab-psh03-15-2920241645321.jpg)
![Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 | Lane 2: RT4 | Lane 3: U-251 MG | Lane 4: Human Plasma | Lane 5: Liver | Lane 6: Tonsil](http://images.novusbio.com/fullsize/nbp3-21294_rabbit-hexokinase-2-pab-4520231552913.jpg)
![Immunocytochemistry/Immunofluorescence: VE-Cadherin Antibody [NBP3-21223] - Staining of human cell line EFO-21 shows localization to nucleoplasm, nuclear membrane & plasma membrane.](http://images.novusbio.com/fullsize/nbp3-21223_rabbit-ve-cadherin-pab-45202315531342.jpg "Immunocytochemistry/Immunofluorescence: VE-Cadherin Antibody [NBP3-21223] - Staining of human cell line EFO-21 shows localization to nucleoplasm, nuclear membrane & plasma membrane.")
![Western Blot: VE-Cadherin Antibody [NBP3-21223] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 | Lane 2: RT4 | Lane 3: U-251 MG | Lane 4: Human Plasma | Lane 5: Liver | Lane 6: Tonsil](http://images.novusbio.com/fullsize/nbp3-21223_rabbit-ve-cadherin-pab-45202315493239.jpg)
![Western Blot: CCAR1 Antibody [NBP3-35368] - Western blot analysis of various lysates using CCAR1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.](http://images.novusbio.com/fullsize/nbp3-35368_rabbit-ccar1-pab-912202415133430.jpg "Western Blot: CCAR1 Antibody [NBP3-35368] - Western blot analysis of various lysates using CCAR1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.")
![Immunoprecipitation: CCAR1 Antibody [NBP3-35368] - Immunoprecipitation analysis of 200 ug extracts of HeLa cells using 3 ug CCAR1 antibody. Western blot was performed from the immunoprecipitate using CCAR1 antibody at a dilution of 1:500.](http://images.novusbio.com/fullsize/nbp3-35368_rabbit-ccar1-pab-9122024141819.jpg)
![Flow Cytometry: VE-Cadherin Antibody (048) [NBP2-89498] - Analysis of Human CD144 expression on HUVEC cells. Cells were stained with purified anti-Human CD144, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/VE-Cadherin-Antibody-048-Flow-Cytometry-NBP2-89498-img0001.jpg "Flow Cytometry: VE-Cadherin Antibody (048) [NBP2-89498] - Analysis of Human CD144 expression on HUVEC cells. Cells were stained with purified anti-Human CD144, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.")
![Immunocytochemistry/Immunofluorescence: VE-Cadherin Antibody (048) [NBP2-89498] - Staining of CDH5 in HUVEC cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-89498 antibody (dilution ratio 1:60) at 4c overnight. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp2-89498_rabbit-monoclonal-ve-cadherin-antibody-048-235202521451786.png)
![Western Blot: CCAR1 Antibody [NB500-188] - Detection of Human CCAR1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CCAR1 antibody NB500-188 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/CCAR1-Antibody-Western-Blot-NB500-188-img0003.jpg "<b>Biological Strategies Validation. </b>Western Blot: CCAR1 Antibody [NB500-188] - Detection of Human CCAR1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CCAR1 antibody NB500-188 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: CCAR1 Antibody [NB500-188] - on HeLa whole cell lysate using NB500-188. CCAR1 was also immunoprecipitated with another rabbit anti-CCAR1 antibody, NB500-187, and NB500-188. Immunoprecipitated CCAR1 was blotted using NB500-188.](http://images.novusbio.com/fullsize/CCAR1-Antibody-Western-Blot-NB500-188-img0001.jpg)
![Western Blot: Hexokinase 2 Antibody [NBP1-90922] - Analysis in human cell line U-87 MG.](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-Western-Blot-NBP1-90922-img0005.jpg "Western Blot: Hexokinase 2 Antibody [NBP1-90922] - Analysis in human cell line U-87 MG.")
