| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38604-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37490-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-13861-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-33730-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-33393-25ul | |||
![Western Blot: Coronin-1a Antibody [NB100-1298] - Staining of Human Lymph node (A) and Spleen (B) lysate (35 ug protein in RIPA buffer). Antibody at 0.5 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Coronin-1a-Antibody-Western-Blot-NB100-1298-img0004.jpg "Western Blot: Coronin-1a Antibody [NB100-1298] - Staining of Human Lymph node (A) and Spleen (B) lysate (35 ug protein in RIPA buffer). Antibody at 0.5 ug/mL. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: Coronin-1a Antibody [NB100-1298] - Staining of paraffin embedded human spleen. Antibody at 3.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Coronin-1a-Antibody-Immunohistochemistry-Paraffin-NB100-1298-img0002.jpg)
![Flow Cytometry: Coronin-1a Antibody [NB100-1298] - Flow cytometric analysis of paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/Coronin-1a-Antibody-Flow-Cytometry-NB100-1298-img0005.jpg)
![Western Blot: Coronin-1a Antibody [NB100-1298] - Staining of Daudi (A), MOLT4 (B) and (1ug/ml) of Jurkat (C) cell lysate (35 ug protein in RIPA buffer). Antibody at 0.5 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Coronin-1a-Antibody-Western-Blot-NB100-1298-img0003.jpg)
![Immunohistochemistry-Paraffin: Coronin-1a Antibody [NB100-1298] - Staining of paraffin embedded human small Intestine. Antibody at 3.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Coronin-1a-Antibody-Immunohistochemistry-Paraffin-NB100-1298-img0001.jpg)
![Immunohistochemistry: Coronin-1a Antibody [NBP3-38604] - Immunohistochemistry analysis of paraffin-embedded Mouse spleen using Coronin-1a Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38604_rabbit-coronin-1a-pab-912202414201354.jpg "Immunohistochemistry: Coronin-1a Antibody [NBP3-38604] - Immunohistochemistry analysis of paraffin-embedded Mouse spleen using Coronin-1a Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.")
![Immunohistochemistry: Coronin-1a Antibody [NBP3-38604] - Immunohistochemistry analysis of paraffin-embedded Human esophageal cancer using Coronin-1a Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38604_rabbit-coronin-1a-pab-912202414201323.jpg)
![Western Blot: Coronin-1a Antibody [NBP3-38604] - Western blot analysis of various lysates using Coronin-1a Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.](http://images.novusbio.com/fullsize/nbp3-38604_rabbit-coronin-1a-pab-912202415152849.jpg)
![Immunohistochemistry: Coronin-1a Antibody [NBP3-38604] - Immunohistochemistry analysis of paraffin-embedded Mouse liver using Coronin-1a Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38604_rabbit-coronin-1a-pab-912202414123825.jpg)
![Immunohistochemistry: Coronin-1a Antibody [NBP3-38604] - Immunohistochemistry analysis of paraffin-embedded Rat spleen using Coronin-1a Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38604_rabbit-coronin-1a-pab-912202415152850.jpg)
![Immunohistochemistry: Coronin-1a Antibody [NBP3-38604] - Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer using Coronin-1a Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38604_rabbit-coronin-1a-pab-91220241412386.jpg)
![Western Blot: NCK1 Antibody (5B7) [NBP2-37490] - Western blot analysis using NCK1 mouse mAb against Jurkat (1), HeLa (2), HEK293 (3), A431 (4), K562 (5), and COS7 (6) cell lysate.](http://images.novusbio.com/fullsize/NCK1-Antibody-5B7-Western-Blot-NBP2-37490-img0003.jpg "Western Blot: NCK1 Antibody (5B7) [NBP2-37490] - Western blot analysis using NCK1 mouse mAb against Jurkat (1), HeLa (2), HEK293 (3), A431 (4), K562 (5), and COS7 (6) cell lysate.")
![Immunohistochemistry: NCK1 Antibody (5B7) [NBP2-37490] - Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using NCK1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/NCK1-Antibody-5B7-Immunohistochemistry-NBP2-37490-img0005.jpg)
![Flow Cytometry: NCK1 Antibody (5B7) [NBP2-37490] - Flow cytometric analysis of Jurkat cells using NCK1 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/NCK1-Antibody-5B7-Flow-Cytometry-NBP2-37490-img0004.jpg)
![Western Blot: NCK1 Antibody (5B7) [NBP2-37490] - Western blot analysis using NCK1 mAb against human NCK1 (AA: 203-371) recombinant protein. (Expected MW is 44.9 kDa)](http://images.novusbio.com/fullsize/NCK1-Antibody-5B7-Western-Blot-NBP2-37490-img0002.jpg)
![ELISA: NCK1 Antibody (5B7) [NBP2-37490] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/NCK1-Antibody-5B7-ELISA-NBP2-37490-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: B7-2/CD86 Antibody (SJ20-00) [NBP2-67417] - Analysis of Raji cells labeling CD86 with Rabbit anti-CD86 antibody at 1/500 dilution.Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CD86 antibody at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594,was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp2-67417_rabbit-monoclonal-b7-2-cd86-antibody-sj20-00-immunocytochemistry-immunofluorescence-2972024113947..jpg "Immunocytochemistry/Immunofluorescence: B7-2/CD86 Antibody (SJ20-00) [NBP2-67417] - Analysis of Raji cells labeling CD86 with Rabbit anti-CD86 antibody at 1/500 dilution.Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CD86 antibody at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594,was used as the secondary antibody at 1/1,000 dilution.")
![Western Blot: B7-2/CD86 Antibody (SJ20-00) [NBP2-67417] - Analysis of CD86 on different lysates with Rabbit anti-CD86 antibody at 1/1,000 dilution.Lane 1: Raji cell lysate (10 ug/Lane)Lane 2: Mouse lung tissue lysate (20 ug/Lane)Lane 3: Rat lung tissue lysate (20 ug/Lane)Predicted band size: 38 kDaObserved band size: 70 kDaExposure time: 1 minute;4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp2-67417_rabbit-monoclonal-b7-2-cd86-antibody-sj20-00-western-blot-2972024114129..jpg)
![Western Blot: B7-2/CD86 Antibody (SJ20-00) [NBP2-67417] - Analysis of CD86 on different lysates with Rabbit anti-CD86 antibody at 1/2,000 dilution.Lane 1: Daudi cell lysateLane 2: Raji cell lysateLane 3: Ramos cell lysateLane 4: Jurkat cell lysateLane 5: K-562 cell lysateLysates/proteins at 15 ug/Lane.Predicted band size: 38 kDaObserved band size: 70 kDaExposure time: 2 minutes 37 seconds;4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp2-67417_rabbit-monoclonal-b7-2-cd86-antibody-sj20-00-western-blot-2972024114258..jpg)
![Western Blot: Uromodulin Antibody [H00007369-B01P] - Analysis of UMOD expression in transfected 293T cell line by UMOD polyclonal antibody. Lane 1: UMOD transfected lysate(67.21 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Uromodulin-Antibody-Western-Blot-H00007369-B01P-img0006.jpg "Western Blot: Uromodulin Antibody [H00007369-B01P] - Analysis of UMOD expression in transfected 293T cell line by UMOD polyclonal antibody. Lane 1: UMOD transfected lysate(67.21 KDa). Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: Uromodulin Antibody [H00007369-B01P] - Analysis of purified antibody to UMOD on HeLa cell. (antibody concentration 10 ug/ml)](http://images.novusbio.com/fullsize/Uromodulin-Antibody-Immunocytochemistry-Immunofluorescence-H00007369-B01P-img0004.jpg)
![Immunohistochemistry-Paraffin: Uromodulin Antibody [H00007369-B01P] - Analysis of purified antibody to UMOD on formalin-fixed paraffin-embedded human kidney. (antibody concentration 3 ug/ml)](http://images.novusbio.com/fullsize/Uromodulin-Antibody-Immunohistochemistry-Paraffin-H00007369-B01P-img0005.jpg)
![Immunohistochemistry-Paraffin: Coronin-1a Antibody [NBP2-13861] - Staining in human lymph node and pancreas tissues using anti-CORO1A antibody. Corresponding CORO1A RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Coronin-1a-Antibody-Immunohistochemistry-Paraffin-NBP2-13861-img0008.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Coronin-1a Antibody [NBP2-13861] - Staining in human lymph node and pancreas tissues using anti-CORO1A antibody. Corresponding CORO1A RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: Coronin-1a Antibody [NBP2-13861] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue](http://images.novusbio.com/fullsize/Coronin-1a-Antibody-Western-Blot-NBP2-13861-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Coronin-1a Antibody [NBP2-13861] - Immunofluorescent staining of human cell line Hep G2 shows localization to cytosol.](http://images.novusbio.com/fullsize/Coronin-1a-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-13861-img0004.jpg)
![Immunohistochemistry-Paraffin: Coronin-1a Antibody [NBP2-13861] - Staining of human lymph node shows high expression.](http://images.novusbio.com/fullsize/Coronin-1a-Antibody-Immunohistochemistry-Paraffin-NBP2-13861-img0006.jpg)
![Immunohistochemistry-Paraffin: Coronin-1a Antibody [NBP2-13861] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/Coronin-1a-Antibody-Immunohistochemistry-Paraffin-NBP2-13861-img0007.jpg)
![Western Blot: NCK1 Antibody (4R1N9) [NBP3-16759] - Western blot analysis of extracts of various cell lines, using NCK11 Rabbit mAb (NBP3-16759) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/NCK1-Antibody-4R1N9-Western-Blot-NBP3-16759-img0003.jpg "Western Blot: NCK1 Antibody (4R1N9) [NBP3-16759] - Western blot analysis of extracts of various cell lines, using NCK11 Rabbit mAb (NBP3-16759) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunocytochemistry/Immunofluorescence: NCK1 Antibody (4R1N9) [NBP3-16759] - Immunofluorescence analysis of NIH-3T3 cells using NCK11 Rabbit mAb (NBP3-16759) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/NCK1-Antibody-4R1N9-Immunocytochemistry-Immunofluorescence-NBP3-16759-img0002.jpg)
![Immunohistochemistry-Paraffin: NCK1 Antibody (4R1N9) [NBP3-16759] - Immunohistochemistry of paraffin-embedded rat testis using NCK11 Rabbit mAb (NBP3-16759) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/NCK1-Antibody-4R1N9-Immunohistochemistry-Paraffin-NBP3-16759-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: NCK1 Antibody (4R1N9) [NBP3-16759] - Immunofluorescence analysis of NIH-3T3 cells using NCK11 Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-16759_rabbit-nck1-mab-4r1n9-52202511374179.jpg)
![Immunohistochemistry-Paraffin: Uromodulin Antibody [NBP2-33730] - Staining in human kidney and cerebral cortex tissues using NBP2-33730 antibody. Corresponding UMOD RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Uromodulin-Antibody-Immunohistochemistry-Paraffin-NBP2-33730-img0013.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Uromodulin Antibody [NBP2-33730] - Staining in human kidney and cerebral cortex tissues using NBP2-33730 antibody. Corresponding UMOD RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Uromodulin Antibody [NBP2-33730] - Staining of human cerebral cortex shows no positivity in neurons as expected.](http://images.novusbio.com/fullsize/Uromodulin-Antibody-Immunohistochemistry-Paraffin-NBP2-33730-img0008.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Uromodulin Antibody [NBP2-33730] - Staining of human cerebral cortex, kidney, placenta and testis using Anti-UMOD antibody NBP2-33730 (A) shows similar protein distribution across tissues to independent antibody NBP2-33393 (B).](http://images.novusbio.com/fullsize/Uromodulin-Antibody-Immunohistochemistry-Paraffin-NBP2-33730-img0009.jpg)
![Immunohistochemistry-Paraffin: Uromodulin Antibody [NBP2-33730] - Staining of human kidney shows moderate cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/Uromodulin-Antibody-Immunohistochemistry-Paraffin-NBP2-33730-img0010.jpg)
![Immunohistochemistry-Paraffin: Uromodulin Antibody [NBP2-33730] - Staining of human placenta shows no positivity in trophoblastic cells as expected.](http://images.novusbio.com/fullsize/Uromodulin-Antibody-Immunohistochemistry-Paraffin-NBP2-33730-img0011.jpg)
![Immunohistochemistry-Paraffin: Uromodulin Antibody [NBP2-33730] - Staining of human testis shows no positivity in cells in seminiferous ducts as expected.](http://images.novusbio.com/fullsize/Uromodulin-Antibody-Immunohistochemistry-Paraffin-NBP2-33730-img0012.jpg)
![Immunohistochemistry-Paraffin: Uromodulin Antibody [NBP2-33393] - Staining in human kidney and cerebral cortex tissues using NBP2-33393 antibody. Corresponding UMOD RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Uromodulin-Antibody-Immunohistochemistry-Paraffin-NBP2-33393-img0019.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Uromodulin Antibody [NBP2-33393] - Staining in human kidney and cerebral cortex tissues using NBP2-33393 antibody. Corresponding UMOD RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: Uromodulin Antibody [NBP2-33393] - Staining of human cerebral cortex, kidney, placenta and testis using Anti-UMOD antibody NBP2-33393 (A) shows similar protein distribution across tissues to independent antibody NBP2-33730 (B).](http://images.novusbio.com/fullsize/Uromodulin-Antibody-Immunohistochemistry-Paraffin-NBP2-33393-img0015.jpg)
![Immunohistochemistry-Paraffin: Uromodulin Antibody [NBP2-33393] - Staining of human cerebral cortex shows no positivity in neurons as expected.](http://images.novusbio.com/fullsize/Uromodulin-Antibody-Immunohistochemistry-Paraffin-NBP2-33393-img0014.jpg)
![Immunohistochemistry-Paraffin: Uromodulin Antibody [NBP2-33393] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/Uromodulin-Antibody-Immunohistochemistry-Paraffin-NBP2-33393-img0016.jpg)
![Immunohistochemistry-Paraffin: Uromodulin Antibody [NBP2-33393] - Staining of human placenta shows no positivity in trophoblastic cells as expected.](http://images.novusbio.com/fullsize/Uromodulin-Antibody-Immunohistochemistry-Paraffin-NBP2-33393-img0017.jpg)
![Immunohistochemistry-Paraffin: Uromodulin Antibody [NBP2-33393] - Staining of human testis shows no positivity in cells in seminiferous ducts as expected.](http://images.novusbio.com/fullsize/Uromodulin-Antibody-Immunohistochemistry-Paraffin-NBP2-33393-img0018.jpg)
![Western Blot: B7-2/CD86 Antibody (BLR029F) [NBP2-76416] - Detection of human CD86 by WB of HDLM-2, MJ, SUP-M2, KG-1, MOLT-4, Ramos, Jurkat, HeLa, HEK293T, and RAW 264.7 lysate. Antibody: Rabbit anti-CD86 recombinant monoclonal [BLR029F] (NBP2-76416). Secondary: HRP-conjugated goat anti-rabbit IgG. Lower Panel: Rabbit anti-GAPDH.](http://images.novusbio.com/fullsize/B7-2-CD86-Antibody-BLR029F-Western-Blot-NBP2-76416-img0002.jpg "Western Blot: B7-2/CD86 Antibody (BLR029F) [NBP2-76416] - Detection of human CD86 by WB of HDLM-2, MJ, SUP-M2, KG-1, MOLT-4, Ramos, Jurkat, HeLa, HEK293T, and RAW 264.7 lysate. Antibody: Rabbit anti-CD86 recombinant monoclonal [BLR029F] (NBP2-76416). Secondary: HRP-conjugated goat anti-rabbit IgG. Lower Panel: Rabbit anti-GAPDH.")
![Immunocytochemistry/Immunofluorescence: B7-2/CD86 Antibody (BLR029F) [NBP2-76416] - Detection of human CD86 in FFPE HDLM-2 cells by ICC. Antibody: Rabbit anti-CD86 recombinant monoclonal [BLR029F] (NBP2-76416) used at 1:200. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/B7-2-CD86-Antibody-BLR029F-Immunocytochemistry-NBP2-76416-img0001.jpg)
![Immunoprecipitation: B7-2/CD86 Antibody (BLR029F) [NBP2-76416] - Detection of human CD86 by WB of immunoprecipitates from HDML-2 lysate. Antibodies: Rabbit anti-CD86 recombinant monoclonal [BLR029F] (NBP2-76416) and rabbit anti-CD86 recombinant monoclonal [BLR030F] (NBP2-76417).](http://images.novusbio.com/fullsize/B7-2-CD86-Antibody-BLR029F-Immunoprecipitation-NBP2-76416-img0003.jpg)
![Immunoprecipitation: B7-2/CD86 Antibody (BLR029F) [NBP2-76416] - Samples: Whole cell lysate (1 mg per IP; 10% of IP loaded) from HDLM-2 cells prepared using NETN lysis buffer. Antibodies: NBP2-76417 Lot 4 used for immunoprecipitation at 6 ul per reaction. B7-2/CD86 was also immunoprecipitated by a previous lot of this antibody (NBP2-76417 Lot 1) and a second antibody, NBP2-76416, against a different epitope of B7-2/CD86 (BLR029F). For blotting immunoprecipitated CD86, NBP2-76417 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 1 second.](http://images.novusbio.com/fullsize/nbp2-76416_rabbit-monoclonal-b7-2-cd86-antibody-blr029f-immunoprecipitation-13120251501..jpg)