| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-11969SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-12182-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-27177SS | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Biotin | NB120-7336-0.1mg | |||
![Flow Cytometry: OX40 Ligand/TNFSF4 Antibody (MM0505-8S23) [NBP2-11969] - An intracellular stain was performed on U-937 cells withOX40 Ligand/TNFSF4 Antibody (MM0505-8S23) NBP2-11969PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.](http://images.novusbio.com/fullsize/OX40-Ligand-TNFSF4-Antibody-MM0505-8S23-Flow-Cytometry-NBP2-11969-img0001.jpg "Flow Cytometry: OX40 Ligand/TNFSF4 Antibody (MM0505-8S23) [NBP2-11969] - An intracellular stain was performed on U-937 cells withOX40 Ligand/TNFSF4 Antibody (MM0505-8S23) NBP2-11969PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.")
![Immunohistochemistry-Paraffin: CD86 Antibody (AP-MAB0803) [NBP2-12182] - 4% PFA fixed and paraffin embedded mouse lung tissue (LPS treated) was subjected to IHC staining using this CD86](http://images.novusbio.com/fullsize/CD86-Antibody-AP-MAB0803-Immunohistochemistry-Paraffin-NBP2-12182-img0001.jpg "<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: CD86 Antibody (AP-MAB0803) [NBP2-12182] - 4% PFA fixed and paraffin embedded mouse lung tissue (LPS treated) was subjected to IHC staining using this CD86")
![Western Blot: beta-III Tubulin Antibody (SP06-00) [NBP2-67374] - Analysis of Beta III Tubulin on different lysates with Rabbit anti-Beta III Tubulin antibody at 1/500 dilution. Lane 1: PC-12 cell lysate, Lane 2: Hela cell lysateLane 3: NIH/3T3 cell lysate, Lane 4: SH-SY5Y cell lysate. Lysates/proteins at 10 ug/Lane. Predicted band size: 50 kDa, Observed band size: 50 kDa. Exposure time: 30 seconds; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-SP06-00-Western-Blot-NBP2-67374-img0006.jpg "Western Blot: beta-III Tubulin Antibody (SP06-00) [NBP2-67374] - Analysis of Beta III Tubulin on different lysates with Rabbit anti-Beta III Tubulin antibody at 1/500 dilution. Lane 1: PC-12 cell lysate, Lane 2: Hela cell lysateLane 3: NIH/3T3 cell lysate, Lane 4: SH-SY5Y cell lysate. Lysates/proteins at 10 ug/Lane. Predicted band size: 50 kDa, Observed band size: 50 kDa. Exposure time: 30 seconds; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: beta-III Tubulin Antibody (SP06-00) [NBP2-67374] - Staining Tubulin beta-III in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-SP06-00-Immunocytochemistry-Immunofluorescence-NBP2-67374-img0002.jpg)
![Immunohistochemistry-Paraffin: beta-III Tubulin Antibody (SP06-00) [NBP2-67374] - Analysis of paraffin-embedded mouse brain tissue using anti-Tubulin beta-III antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-SP06-00-Immunohistochemistry-Paraffin-NBP2-67374-img0003.jpg)
![Flow Cytometry: beta-III Tubulin Antibody (SP06-00) [NBP2-67374] - Flow cytometric analysis of beta-III Tubulin was done on N2A cells. The cells were fixed, permeabilized and stained with the primary antibody (1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes. Unlabeled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-SP06-00-Flow-Cytometry-NBP2-67374-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: beta-III Tubulin Antibody (SP06-00) [NBP2-67374] - Staining Tubulin beta-III in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-SP06-00-Immunocytochemistry-Immunofluorescence-NBP2-67374-img0001.jpg)
![Western Blot: Jak2 Antibody (6-D3) [NBP2-66913] - Analysis on K562 cell lysate using anti-JAK2 mouse mAb.](http://images.novusbio.com/fullsize/Jak2-Antibody-6-D3-Western-Blot-NBP2-66913-img0003.jpg "Western Blot: Jak2 Antibody (6-D3) [NBP2-66913] - Analysis on K562 cell lysate using anti-JAK2 mouse mAb.")
![Immunocytochemistry/Immunofluorescence: Jak2 Antibody (6-D3) [NBP2-66913] - Staining JAK2 in HeLa cells (red). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X-100, PBS.](http://images.novusbio.com/fullsize/Jak2-Antibody-6-D3-Immunocytochemistry-Immunofluorescence-NBP2-66913-img0001.jpg)
![Immunohistochemistry-Paraffin: Jak2 Antibody (6-D3) [NBP2-66913] - Analysis of paraffin-embedded human breast carcinoma tissue using anti- JAK2 mouse mAb.](http://images.novusbio.com/fullsize/Jak2-Antibody-6-D3-Immunohistochemistry-Paraffin-NBP2-66913-img0002.jpg)
![Microarray: Jak2 Antibody (6-D3) [NBP2-66913] - Antibody was printed on custom arrays and incubated with fluorescently labeled human EDTA plasma. Microarray image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Jak2-Antibody-6-D3-Microarray-NBP2-66913-img0004.jpg)
![Western Blot: Jak2 Antibody (6-D3) [NBP2-66913] -Analysis on K562 cell lysates using anti-JAK2 mouse mAb.](http://images.novusbio.com/fullsize/nbp2-66913_mouse-monoclonal-jak2-antibody-6-d3-western-blot-187202411641..jpg)
![Western Blot: Jak2 [p Tyr1007, p Tyr1008] Antibody (SY24-03) [NBP2-67429] - ICC staining of Phospho-JAK2(Y1007+Y1008) in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor (TM) 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).](http://images.novusbio.com/fullsize/Jak2-p-Tyr1007-p-Tyr1008-Antibody-SY24-03-Western-Blot-NBP2-67429-img0006.jpg "Western Blot: Jak2 [p Tyr1007, p Tyr1008] Antibody (SY24-03) [NBP2-67429] - ICC staining of Phospho-JAK2(Y1007+Y1008) in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor (TM) 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).")
![Immunocytochemistry/Immunofluorescence: Jak2 [p Tyr1007, p Tyr1008] Antibody (SY24-03) [NBP2-67429] - Staining Phospho-JAK2(Y1007+Y1008) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Jak2-p-Tyr1007-p-Tyr1008-Antibody-SY24-03-Immunocytochemistry-Immunofluorescence-NBP2-67429-img0001.jpg)
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: Jak2 [p Tyr1007, p Tyr1008] Antibody (SY24-03) [NBP2-67429] - Analysis of paraffin-embedded mouse kidney tissue using anti-Jak2 [p Tyr1007, p Tyr1008] antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Jak2-p-Tyr1007-p-Tyr1008-Antibody-SY24-03-Immunohistochemistry-Paraffin-NBP2-67429-img0007.jpg)
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: Jak2 [p Tyr1007, p Tyr1008] Antibody (SY24-03) [NBP2-67429] - Analysis of paraffin-embedded human lung carcinoma tissue using anti-Phospho-JAK2(Y1007+Y1008) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX](http://images.novusbio.com/fullsize/Jak2-p-Tyr1007-p-Tyr1008-Antibody-SY24-03-Immunohistochemistry-Paraffin-NBP2-67429-img0002.jpg)
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: Jak2 [p Tyr1007, p Tyr1008] Antibody (SY24-03) [NBP2-67429] - Human tonsil tissue using anti-Phospho-JAK2(Y1007+Y1008) antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Jak2-p-Tyr1007-p-Tyr1008-Antibody-SY24-03-Immunohistochemistry-Paraffin-NBP2-67429-img0003.jpg)
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: Jak2 [p Tyr1007, p Tyr1008] Antibody (SY24-03) [NBP2-67429] - Human kidney tissue using anti-Phospho-JAK2(Y1007+Y1008) antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Jak2-p-Tyr1007-p-Tyr1008-Antibody-SY24-03-Immunohistochemistry-Paraffin-NBP2-67429-img0004.jpg)
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: Jak2 [p Tyr1007, p Tyr1008] Antibody (SY24-03) [NBP2-67429] - Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-Jak2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1607-34, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Jak2-p-Tyr1007-p-Tyr1008-Antibody-SY24-03-Immunohistochemistry-Paraffin-NBP2-67429-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: B7-2/CD86 Antibody (SJ20-00) [NBP2-67417] - Analysis of Raji cells labeling CD86 with Rabbit anti-CD86 antibody at 1/500 dilution.Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CD86 antibody at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594,was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp2-67417_rabbit-monoclonal-b7-2-cd86-antibody-sj20-00-immunocytochemistry-immunofluorescence-2972024113947..jpg "Immunocytochemistry/Immunofluorescence: B7-2/CD86 Antibody (SJ20-00) [NBP2-67417] - Analysis of Raji cells labeling CD86 with Rabbit anti-CD86 antibody at 1/500 dilution.Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CD86 antibody at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594,was used as the secondary antibody at 1/1,000 dilution.")
![Western Blot: B7-2/CD86 Antibody (SJ20-00) [NBP2-67417] - Analysis of CD86 on different lysates with Rabbit anti-CD86 antibody at 1/1,000 dilution.Lane 1: Raji cell lysate (10 ug/Lane)Lane 2: Mouse lung tissue lysate (20 ug/Lane)Lane 3: Rat lung tissue lysate (20 ug/Lane)Predicted band size: 38 kDaObserved band size: 70 kDaExposure time: 1 minute;4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp2-67417_rabbit-monoclonal-b7-2-cd86-antibody-sj20-00-western-blot-2972024114129..jpg)
![Western Blot: B7-2/CD86 Antibody (SJ20-00) [NBP2-67417] - Analysis of CD86 on different lysates with Rabbit anti-CD86 antibody at 1/2,000 dilution.Lane 1: Daudi cell lysateLane 2: Raji cell lysateLane 3: Ramos cell lysateLane 4: Jurkat cell lysateLane 5: K-562 cell lysateLysates/proteins at 15 ug/Lane.Predicted band size: 38 kDaObserved band size: 70 kDaExposure time: 2 minutes 37 seconds;4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp2-67417_rabbit-monoclonal-b7-2-cd86-antibody-sj20-00-western-blot-2972024114258..jpg)
![Western Blot: AIF Antibody [NBP2-27177] - Western blot shows lysates of HEK293T human embryonic kidney parental cell line and AIF knockout (KO) HEK293T cell line. PVDF membrane was probed with 1:1000 of Rabbit Anti-Human AIF Polyclonal Antibody (Catalog # NBP2-27177) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for HDAC1 at approximately 68 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in the knockout HEK293T cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/AIF-Antibody-Knockout-Validated-NBP2-27177-img0005.jpg "<b>Genetic Strategies Validation. </b>Western Blot: AIF Antibody [NBP2-27177] - Western blot shows lysates of HEK293T human embryonic kidney parental cell line and AIF knockout (KO) HEK293T cell line. PVDF membrane was probed with 1:1000 of Rabbit Anti-Human AIF Polyclonal Antibody (Catalog # NBP2-27177) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for HDAC1 at approximately 68 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in the knockout HEK293T cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: AIF Antibody [NBP2-27177] - Analysis of AIF in human colon using this antibody. Lane 1, normal colon. Lanes 2-7, colon carcinoma tissue lysatesfrom 6 different patients. mAIF, thought to represent mitochondrial AIF.](http://images.novusbio.com/fullsize/AIF-Antibody-Western-Blot-NBP2-27177-img0001.jpg)
![Immunohistochemistry-Paraffin: AIF Antibody [NBP2-27177] - Staining of AIF in formalin-fixed, paraffin-embedded normal human colon using this antibody at 1:2000. Hematoxylin-eosin counterstain. In this example, AIF expression is predominant in the upper part of the crypt.](http://images.novusbio.com/fullsize/AIF-Antibody-Immunohistochemistry-Paraffin-NBP2-27177-img0002.jpg)
![Simple Western: AIF Antibody [NBP2-27177] - Simple Western lane view shows a specific band for AIF in 0.2 mg/ml of Jurkat and MCF-7 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. *Non-specific interaction with the 230 kDa standard may be seen with this antibody.](http://images.novusbio.com/fullsize/AIF-Antibody-Simple-Western-NBP2-27177-img0003.jpg)
![Western Blot: Neurofibromin 1 Antibody [NB100-418] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NF1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.](http://images.novusbio.com/fullsize/Neurofibromin-1-Antibody-Western-Blot-NB100-418-img0005.jpg "<b>Biological Strategies Validation. </b>Western Blot: Neurofibromin 1 Antibody [NB100-418] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NF1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.")
![<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Western Blot: Neurofibromin 1 Antibody [NB100-418] - Detection of human NF1 in Western Blot and IP, using NB100-418. Samples: mock treated (control) or NF1-siRNA treated HeLa WCE. IPed NF was detected using an antibody from another vendor.](http://images.novusbio.com/fullsize/Neurofibromin-1-Antibody-Western-Blot-NB100-418-img0004.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: Neurofibromin 1 Antibody [NB100-418] - Detection of human NF1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NF1 antibody NB100-418 used for IP at 6 ug per reaction. NF1 was also immunoprecipitated by another rabbit anti-NF1 antibody. For blotting immunoprecipitated NF1, NB100-418 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Neurofibromin-1-Antibody-Immunoprecipitation-NB100-418-img0006.jpg)
![Neurofibromin loss sensitizes MEF cells to growth factor and matrix stimulation: (a) MEF cells isolated and immortalized from wild-type and knockout mice for Nf1 gene were analyzed by Western blotting with anti-neurofibromin (Nfn) antibodies; (b) focal adhesion kinase FAK binds to Nfn. FAK was immunoprecipitated (IP) from cell lysates of wild-type and knockout MEFs (Nf1+/+, Nf1−/−); following SDS-PAGE electrophoresis, co-immunoprecipitated Nfn were assayed by Western blotting analysis (IB) with anti-FAK and anti-Nfn antibodies. Total protein loaded on the IP is also shown as INPUT ((b), lower panel) and examined by anti-Nfn and anti-actin antibodies; (c) transient phosphorylation kinetics of MEFs stimulated with PDGF-BB (10 ng/mL), [38,39] for increasing time periods. Following SDS-PAGE, cell lysates were incubated with anti-phospho-Erk (1/2) (T202/Y204, T185/Y187) MAP kinase, phospho-FAK (Y397) and phospho-Src (Y416) antibodies and anti-GAPDH; (d,e) colony formation assay of MEFs fully embedded by low growth factor containing Matrigel in absence (upper panel) or presence (lower panel) of insoluble matrix proteins Collagen I/Fibronectin. Cells were seeded in the Matrigel and 24 h later treated or not with PDGF-BB ligand for 8 days (representative microscope pictures (10×)). Where indicated, MEF cells were additionally treated with FAK and MEK inhibitors named Y10 (0.62 μM) and Selumetinib (6.62 μM), respectively; the area of colonies was calculated in five fields for each well, and mean value and SD calculated and plotted in the right histograms. n = 8; * p < 0.05 **; p < 0.001 by Student’s t-test. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/34066061), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-418_rabbit-polyclonal-neurofibromin-1-antibody-western-blot-132202618215117.jpg)
![Neurofibromin loss sensitizes MEF cells to growth factor and matrix stimulation: (a) MEF cells isolated and immortalized from wild-type and knockout mice for Nf1 gene were analyzed by Western blotting with anti-neurofibromin (Nfn) antibodies; (b) focal adhesion kinase FAK binds to Nfn. FAK was immunoprecipitated (IP) from cell lysates of wild-type and knockout MEFs (Nf1+/+, Nf1−/−); following SDS-PAGE electrophoresis, co-immunoprecipitated Nfn were assayed by Western blotting analysis (IB) with anti-FAK and anti-Nfn antibodies. Total protein loaded on the IP is also shown as INPUT ((b), lower panel) and examined by anti-Nfn and anti-actin antibodies; (c) transient phosphorylation kinetics of MEFs stimulated with PDGF-BB (10 ng/mL), [38,39] for increasing time periods. Following SDS-PAGE, cell lysates were incubated with anti-phospho-Erk (1/2) (T202/Y204, T185/Y187) MAP kinase, phospho-FAK (Y397) and phospho-Src (Y416) antibodies and anti-GAPDH; (d,e) colony formation assay of MEFs fully embedded by low growth factor containing Matrigel in absence (upper panel) or presence (lower panel) of insoluble matrix proteins Collagen I/Fibronectin. Cells were seeded in the Matrigel and 24 h later treated or not with PDGF-BB ligand for 8 days (representative microscope pictures (10×)). Where indicated, MEF cells were additionally treated with FAK and MEK inhibitors named Y10 (0.62 μM) and Selumetinib (6.62 μM), respectively; the area of colonies was calculated in five fields for each well, and mean value and SD calculated and plotted in the right histograms. n = 8; * p < 0.05 **; p < 0.001 by Student’s t-test. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/34066061), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-92100_rabbit-polyclonal-mat2a-antibody-western-blot-13220261739820.jpg)
![Western Blot: Host Cell Factor 1/HCFC1 Antibody [NB100-68209] - Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-HCF1 antibody NB100-68209 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. HCF1 was also immunoprecipitated by rabbit anti-HCF1 antibody NB100-68210, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).](http://images.novusbio.com/fullsize/Host-Cell-Factor-1-HCFC1-Antibody-Western-Blot-NB100-68209-img0010.jpg "<b>Independent Antibodies Validation. </b>Western Blot: Host Cell Factor 1/HCFC1 Antibody [NB100-68209] - Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-HCF1 antibody NB100-68209 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. HCF1 was also immunoprecipitated by rabbit anti-HCF1 antibody NB100-68210, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).")
![Western Blot: Host Cell Factor 1/HCFC1 Antibody [NB100-68209] - 5-FU-produced ROS involved in histone methyltransferase-mediated Nrf2 expression. E. Expression patterns of TET1, OGT, HCF1, & MLL in NAC pre-treated SNUC5/5-FUR cells determined by WB analysis. *Significantly different from SNUC5 cells (p<0.05), & #significantly different from SNUC5/5-FUR cells (p<0.05). Also, SNUC5 cells pre-treated with 500 μM of NAC & incubated for an additional 1 h at 37°. Cells then treated with 140 μM 5-FU for 48 h. 10 μM of DHR123 was added to plate & incubated for an additional 30 min at 37°C. Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.9745), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-68209_rabbit-polyclonal-host-cell-factor-1-hcfc1-antibody-310202415171343.jpg)
![Western Blot: Host Cell Factor 1/HCFC1 Antibody [NB100-68209] - 5-FU-produced ROS involved in histone methyltransferase-mediated Nrf2 expression. J. Expression of TET1 & interaction proteins detected by WB analysis. *Significantly different from SNUC5 cells (p<0.05), & #significantly different from 5-FU-treated SNUC5 cells (p<0.05). Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.9745), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-68209_rabbit-polyclonal-host-cell-factor-1-hcfc1-antibody-310202415345312.jpg)
![Western Blot: Host Cell Factor 1/HCFC1 Antibody [NB100-68209] - 5-FU-produced ROS involved in histone methyltransferase-mediated Nrf2 expression. F. The intracellular ROS level was detected by flow cytometry. Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.9745), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-68209_rabbit-polyclonal-host-cell-factor-1-hcfc1-antibody-31020241534395.jpg)
![Western Blot: Host Cell Factor 1/HCFC1 Antibody [NB100-68209] - Effect of MLL-HCF1 interaction on histone methylation & Nrf2 expression in SNUC5/5-FUR cellsA. HCF1 expression was determined by Western blotting. *Significantly different from SNUC5 cells (p<0.05). B. Interaction between MLL & HCF1was examined by immunoprecipitation analysis using anti-MLL & anti-HCF1 antibodies followed by Western blotting with anti-HCF1 & anti-MLL antibodies. *Significantly different from SNUC5 cells (p<0.05). C. Interaction between MLL & HCF1was assessed by PLA. Each green spot represents for a single interaction (MLL & HCF1) & DNA was stained with DAPI. *Significantly different from SNUC5 cells (p<0.05). The cells were transfected with non-targeting siRNA (siControl) or siHCF1 RNA for 24 h. D. Expression pattern of MLL mRNA in SNUC5/5-FUR cells was determined by RT-PCR analysis. *Significantly different from siControl-transfected cells (p<0.05). E. Expression pattern of HCF1, MLL, & Nrf2 in SNUC5/5-FUR cells was determined by Western blot analysis. *Significantly different from siControl-transfected cells (p<0.05). Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.9745), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-68209_rabbit-polyclonal-host-cell-factor-1-hcfc1-antibody-310202415532061.jpg)
![Western Blot: Host Cell Factor 1/HCFC1 Antibody [NB100-68209] - Relation of OGT-TET1 interaction on histone methylation & Nrf2 expression in SNUC5/5-FUR cellsA. Interaction between OGT & TET1 was examined by immunoprecipitation analyses using anti-OGT & anti-TET1 antibodies followed by Western blotting with anti-TET1 & anti-OGT antibodies. B. Interaction between OGT & TET1 was assessed by using PLA. Each green spot represents for a single interaction (OGT & TET1) & DNA was stained with DAPI. *Significantly different from SNUC5 cells (p<0.05). C. The O-GlcNAcylated TET1 or O-GlcNAcylated OGT was examined by immunoprecipitation analyses using anti-O-GlcNAc antibody followed by Western blotting with anti-TET1 & anti-OGT antibodies. The cells were transfected with non-targeting siRNA (siControl) or siOGT RNA or siTET1 RNA for 24 h. D. Expression pattern of OGT & TET1 in SNUC5/5-FUR cells was determined by Western blot analysis. *Significantly different from siControl-transfected cells (p<0.05). E. Expression pattern of TET1, OGT, HCF1, MLL, & Nrf2 in SNUC5/5-FUR cells was determined by Western blot analysis. *Significantly different from siControl-transfected cells (p<0.05). Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.9745), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-68209_rabbit-polyclonal-host-cell-factor-1-hcfc1-antibody-310202415523922.jpg)
![Western Blot: Host Cell Factor 1/HCFC1 Antibody [NB100-68209] - Involvement of OGT in histone methylase MLL/COMPASS-like complex-mediated Nrf2 expression in SNUC5/5-FUR cellsA. OGT & O-GlcNAc expression was determined by Western blotting. *Significantly different from SNUC5 cells (p<0.05). B. Interaction between HCF1 & OGT was examined by immunoprecipitation analysis using anti-HCF1 & anti-OGT antibodies followed by Western blotting with anti-HCF1 & anti-OGT antibodies. C. Interaction between HCF1 & OGT was assessed by using PLA. Each green spot represents for a single interaction (HCF1 & OGT) & DNA was stained with DAPI. *Significantly different from SNUC5 cells (p<0.05). D. Interaction between MLL & OGT was examined by immunoprecipitation analysis using anti-MLL & anti-OGT antibodies followed by Western blotting with anti-OGT & anti-MLL antibodies. E. Interaction between MLL & OGT was assessed by using PLA. Each green spot represents for a single interaction (MLL & OGT) & DNA was stained with DAPI. *Significantly different from SNUC5 cells (p<0.05). The cells were transfected with non-targeting siRNA (siControl) or siOGT RNA for 24 h. F. Expression pattern of MLL mRNA in SNUC5/5-FUR cells was determined by RT-PCR analysis. *Significantly different from siControl-transfected cells (p<0.05). G. Expression pattern of OGT, MLL, HFC1, & Nrf2 in SNUC5/5-FUR cells was determined by Western blot analysis. *Significantly different from siControl-transfected cells (p<0.05). Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.9745), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-68209_rabbit-polyclonal-host-cell-factor-1-hcfc1-antibody-310202415532084.jpg)
![Western Blot: Host Cell Factor 1/HCFC1 Antibody [NB100-68209] - Involvement of OGT in histone methylase MLL/COMPASS-like complex-mediated Nrf2 expression in SNUC5/5-FUR cellsA. OGT & O-GlcNAc expression was determined by Western blotting. *Significantly different from SNUC5 cells (p<0.05). B. Interaction between HCF1 & OGT was examined by immunoprecipitation analysis using anti-HCF1 & anti-OGT antibodies followed by Western blotting with anti-HCF1 & anti-OGT antibodies. C. Interaction between HCF1 & OGT was assessed by using PLA. Each green spot represents for a single interaction (HCF1 & OGT) & DNA was stained with DAPI. *Significantly different from SNUC5 cells (p<0.05). D. Interaction between MLL & OGT was examined by immunoprecipitation analysis using anti-MLL & anti-OGT antibodies followed by Western blotting with anti-OGT & anti-MLL antibodies. E. Interaction between MLL & OGT was assessed by using PLA. Each green spot represents for a single interaction (MLL & OGT) & DNA was stained with DAPI. *Significantly different from SNUC5 cells (p<0.05). The cells were transfected with non-targeting siRNA (siControl) or siOGT RNA for 24 h. F. Expression pattern of MLL mRNA in SNUC5/5-FUR cells was determined by RT-PCR analysis. *Significantly different from siControl-transfected cells (p<0.05). G. Expression pattern of OGT, MLL, HFC1, & Nrf2 in SNUC5/5-FUR cells was determined by Western blot analysis. *Significantly different from siControl-transfected cells (p<0.05). Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.9745), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-68209_rabbit-polyclonal-host-cell-factor-1-hcfc1-antibody-310202415525241.jpg)
![Western Blot: Host Cell Factor 1/HCFC1 Antibody [NB100-68209] - Effect of MLL-HCF1 interaction on histone methylation & Nrf2 expression in SNUC5/5-FUR cellsA. HCF1 expression was determined by Western blotting. *Significantly different from SNUC5 cells (p<0.05). B. Interaction between MLL & HCF1was examined by immunoprecipitation analysis using anti-MLL & anti-HCF1 antibodies followed by Western blotting with anti-HCF1 & anti-MLL antibodies. *Significantly different from SNUC5 cells (p<0.05). C. Interaction between MLL & HCF1was assessed by PLA. Each green spot represents for a single interaction (MLL & HCF1) & DNA was stained with DAPI. *Significantly different from SNUC5 cells (p<0.05). The cells were transfected with non-targeting siRNA (siControl) or siHCF1 RNA for 24 h. D. Expression pattern of MLL mRNA in SNUC5/5-FUR cells was determined by RT-PCR analysis. *Significantly different from siControl-transfected cells (p<0.05). E. Expression pattern of HCF1, MLL, & Nrf2 in SNUC5/5-FUR cells was determined by Western blot analysis. *Significantly different from siControl-transfected cells (p<0.05). Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.9745), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-68209_rabbit-polyclonal-host-cell-factor-1-hcfc1-antibody-310202415523923.jpg)
![Western Blot: Host Cell Factor 1/HCFC1 Antibody [NB100-68209] - Effect of MLL-HCF1 interaction on histone methylation & Nrf2 expression in SNUC5/5-FUR cellsA. HCF1 expression was determined by Western blotting. *Significantly different from SNUC5 cells (p<0.05). B. Interaction between MLL & HCF1was examined by immunoprecipitation analysis using anti-MLL & anti-HCF1 antibodies followed by Western blotting with anti-HCF1 & anti-MLL antibodies. *Significantly different from SNUC5 cells (p<0.05). C. Interaction between MLL & HCF1was assessed by PLA. Each green spot represents for a single interaction (MLL & HCF1) & DNA was stained with DAPI. *Significantly different from SNUC5 cells (p<0.05). The cells were transfected with non-targeting siRNA (siControl) or siHCF1 RNA for 24 h. D. Expression pattern of MLL mRNA in SNUC5/5-FUR cells was determined by RT-PCR analysis. *Significantly different from siControl-transfected cells (p<0.05). E. Expression pattern of HCF1, MLL, & Nrf2 in SNUC5/5-FUR cells was determined by Western blot analysis. *Significantly different from siControl-transfected cells (p<0.05). Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.9745), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-68209_rabbit-polyclonal-host-cell-factor-1-hcfc1-antibody-310202415533831.jpg)
![Immunocytochemistry/Immunofluorescence: NCAPH Antibody [NBP1-88345] - Staining of human cell line U-2 OS shows localization to nucleus and cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/NCAPH-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88345-img0017.jpg "Immunocytochemistry/Immunofluorescence: NCAPH Antibody [NBP1-88345] - Staining of human cell line U-2 OS shows localization to nucleus and cytosol. Antibody staining is shown in green.")
![Simple Western: NCAPH Antibody [NBP1-88345] - Simple Western lane view shows a specific band for NCAPH in 0.2 mg/ml of RT-4 (Left) and U-251MG (Right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/NCAPH-Antibody-Simple-Western-NBP1-88345-img0006.jpg)
![Simple Western: NCAPH Antibody [NBP1-88345] - Electropherogram image(s) of corresponding Simple Western lane view. NCAPH antibody was used at 1:20 dilution on RT-4 and U-251MG lysate(s).](http://images.novusbio.com/fullsize/NCAPH-Antibody-Simple-Western-NBP1-88345-img0007.jpg)
![Western Blot: Cyclin D1 Antibody [NBP2-24695] - Analysis of 1) mouse C2C12 and 2) mouse NIH 3T3 lysate using Cyclin D1 antibody at 5 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/Cyclin-D1-Antibody-Western-Blot-NBP2-24695-img0004.jpg)
![Immunohistochemistry-Paraffin: Cyclin D1 Antibody [NBP2-24695] - Formalin-fixed, paraffin-embedded human small intestine stained with Cyclin D1 antibody at 10 ug/ml.](http://images.novusbio.com/fullsize/Cyclin-D1-Antibody-Immunohistochemistry-Paraffin-NBP2-24695-img0005.jpg)
![Western Blot: Plasminogen Antibody [NB600-930] - Lane 1: Plasminogen. Lane 2: None. Load: 50 ng per lane. Primary antibody: Plasminogen primary antibody at 1:1,000 overnight at 4C. Secondary antibody: Peroxidase goat secondary antibody at 1:40,000 for 60 min at RT. Blocking: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 91 kDa, 91 kDa for Plasminogen. Other band(s): None.](http://images.novusbio.com/fullsize/Plasminogen-Antibody-Western-Blot-NB600-930-img0006.jpg "Western Blot: Plasminogen Antibody [NB600-930] - Lane 1: Plasminogen. Lane 2: None. Load: 50 ng per lane. Primary antibody: Plasminogen primary antibody at 1:1,000 overnight at 4C. Secondary antibody: Peroxidase goat secondary antibody at 1:40,000 for 60 min at RT. Blocking: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 91 kDa, 91 kDa for Plasminogen. Other band(s): None.")
![Western Blot: Plasminogen Antibody [NB600-930] - Detection of Plasminogen under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of 1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody. Detection shown was using Dylight 649 conjugated Donkey anti goat 1 hr RT.](http://images.novusbio.com/fullsize/Plasminogen-Antibody-Western-Blot-NB600-930-img0005.jpg)
