| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-06419-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-12080-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-81058-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-20131-0.025mg | |||
![Western Blot: CCDC5 Antibody [NBP3-06419] - CCDC5 Antibody was immunoprecipitated using: Lane A:0.5 mg U937 Whole Cell Lysate. 4 uLCCDC5 Antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: CCDC5 Antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Observed band size :32 kDa](http://images.novusbio.com/fullsize/CCDC5-Antibody-Western-Blot-NBP3-06419-img0003.jpg "Western Blot: CCDC5 Antibody [NBP3-06419] - CCDC5 Antibody was immunoprecipitated using: Lane A:0.5 mg U937 Whole Cell Lysate. 4 uLCCDC5 Antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: CCDC5 Antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Observed band size :32 kDa")
![Western Blot: CCDC5 Antibody [NBP3-06419] - CCDC5 antibody at 1:500 dilution. Lane A: HL60 Whole Cell Lysate Lane B: U937 Whole Cell Lysate Lane C: MOLT4 Whole Cell Lysate Lane D: Daudi Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Observed band size:32 kDa](http://images.novusbio.com/fullsize/CCDC5-Antibody-Western-Blot-NBP3-06419-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: CCDC5 Antibody [NBP3-06419] - Immunofluorescence staining of CCDC5 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with NBP3-06419 antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.](http://images.novusbio.com/fullsize/nbp3-06419_rabbit-polyclonal-ccdc5-antibody-23520252135125.png)
![Western Blot: EGFR [p Tyr1045] Antibody (PSH03-28) [NBP3-32760] - Western blot analysis of EGFR on different lysates with Rabbit anti-EGFR antibody (NBP3-32760) at 1/1,000 dilution. Lane 1: A431 cell lysate Lane 2: A431 treated with 100ng/mL EGF for 30 minutes cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 134 kDa Observed band size: 170 kDa Exposure time: 3 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32760) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32760_rabbit-egfr-p-tyr1045-mab-psh03-28-288202412461681.jpg "Western Blot: EGFR [p Tyr1045] Antibody (PSH03-28) [NBP3-32760] - Western blot analysis of EGFR on different lysates with Rabbit anti-EGFR antibody (NBP3-32760) at 1/1,000 dilution. Lane 1: A431 cell lysate Lane 2: A431 treated with 100ng/mL EGF for 30 minutes cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 134 kDa Observed band size: 170 kDa Exposure time: 3 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32760) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: EGFR [p Tyr1045] Antibody (PSH03-28) [NBP3-32760] - Immunocytochemistry analysis of A431 treated with or without 100ng/mL EGF for 60 minutes cells labeling EGFR with Rabbit anti-EGFR antibody (NBP3-32760) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-EGFR antibody (NBP3-32760) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32760_rabbit-egfr-p-tyr1045-mab-psh03-28-29202410484836.jpg)
![Flow Cytometry: EGFR [p Tyr1045] Antibody (PSH03-28) [NBP3-32760] - Flow cytometric analysis of A431 treated with or without 100ng/mL EGF for 60 minutes cells labeling EGFR. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32760, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32760_rabbit-egfr-p-tyr1045-mab-psh03-28-29202416453235.jpg)
![Western Blot: VE-Cadherin Antibody (PSH03-15) [NBP3-33088] - Western blot analysis of VE-Cadherin on different lysates with Rabbit anti-VE-Cadherin antibody (NBP3-33088) at 1/2,000 dilution. Lane 1: EA.hy926-si NT cell lysate Lane 2: EA.hy926-si VE Cadherin cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 88 kDa Observed band size: 140 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33088) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33088_rabbit-ve-cadherin-mab-psh03-15-288202414191513.jpg "Western Blot: VE-Cadherin Antibody (PSH03-15) [NBP3-33088] - Western blot analysis of VE-Cadherin on different lysates with Rabbit anti-VE-Cadherin antibody (NBP3-33088) at 1/2,000 dilution. Lane 1: EA.hy926-si NT cell lysate Lane 2: EA.hy926-si VE Cadherin cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 88 kDa Observed band size: 140 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33088) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: VE-Cadherin Antibody (PSH03-15) [NBP3-33088] - Immunocytochemistry analysis of HeLa cells labeling VE-Cadherin with Rabbit anti-VE-Cadherin antibody (NBP3-33088) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-VE-Cadherin antibody (NBP3-33088) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-33088_rabbit-ve-cadherin-mab-psh03-15-29202410234272.jpg)
![Flow Cytometry: VE-Cadherin Antibody (PSH03-15) [NBP3-33088] - Flow cytometric analysis of EA.hy926 cells labeling VE-Cadherin. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (NBP3-33088, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-33088_rabbit-ve-cadherin-mab-psh03-15-2920241645321.jpg)
![Western Blot: EGF R/ErbB1 [p Tyr992] Antibody (EM-12) [NBP1-44894] - Analysis of EGFR (phospho-Tyr992) by mouse monoclonal antibody EM-12 in EGF-treated A431 (A), CALU-3 (B), MCF-7 (C), Jurkat (D) and Ramos (E) cell lines (reduced conditions).](http://images.novusbio.com/fullsize/EGF-R-ErbB1-p-Tyr992-Antibody-EM-12-Western-Blot-NBP1-44894-img0001.jpg "Western Blot: EGF R/ErbB1 [p Tyr992] Antibody (EM-12) [NBP1-44894] - Analysis of EGFR (phospho-Tyr992) by mouse monoclonal antibody EM-12 in EGF-treated A431 (A), CALU-3 (B), MCF-7 (C), Jurkat (D) and Ramos (E) cell lines (reduced conditions).")
![Immunoprecipitation: EGF R/ErbB1 [p Tyr992] Antibody (EM-12) [NBP1-44894] - EGFR from EGF-treated A431 cells by monoclonal antibodies EM-12 (A), EM-13 (B), a commercial anti-EGFR polyclonal antibody (C) and anti-EGFR monoclonal (D). The precipitates were immunoblotted with EM-12 or EM-13 antibody, and goat anti-mouse-HRP.](http://images.novusbio.com/fullsize/EGF-R-ErbB1-p-Tyr992-Antibody-EM-12-Immunoprecipitation-NBP1-44894-img0002.jpg)
![Immunoprecipitation: EGF R/ErbB1 [p Tyr992] Antibody (EM-12) [NBP1-44894] - EGFR from EGF-treated A431 cells by phosphospecific monoclonal antibodies EM-12 (A), EM-13 (B), a commercial anti-EGFR polyclonal antibody (C) and anti-EGFR monoclonal (D). The precipitates were immunoblotted with a commercial anti-EGFR polyclonal and goat anti-rabbit-HRP.](http://images.novusbio.com/fullsize/EGF-R-ErbB1-p-Tyr992-Antibody-EM-12-Immunoprecipitation-NBP1-44894-img0003.jpg)
![Western Blot: ERK1/2 Antibody (B20-U) [NBP1-30128] - Left Lane: Marker, Right Lane: Rat brain crude lysate (100ug of protein loaded).](http://images.novusbio.com/fullsize/ERK1-2-Antibody-B20-U-Western-Blot-NBP1-30128-img0001.jpg "Western Blot: ERK1/2 Antibody (B20-U) [NBP1-30128] - Left Lane: Marker, Right Lane: Rat brain crude lysate (100ug of protein loaded).")
![Immunocytochemistry/Immunofluorescence: ERK1/2 Antibody (B20-U) [NBP1-30128] - Expression in HEK293 cells.](http://images.novusbio.com/fullsize/ERK1-2-Antibody-B20-U-Immunocytochemistry-Immunofluorescence-NBP1-30128-img0005.jpg)
![Western Blot: EB2 Antibody (KT52) [NBP3-23289] - Western blot of EB proteins in mouse brain (lanes 1-5). The blot was probed with rat monoclonals anti-EB1 (lane 1), anti-EB1/2/3 (lane 2), NBP3-23289 anti-EB2 (lane 3), NBP3-23293 anti-EB3 (lane 4), and NBP3-23294 anti-EB3 (lane 5). Then the antibodies were detected using goat anti-Rat IgG Light Chain specific: HRP.](http://images.novusbio.com/fullsize/nbp3-23289_rat-eb2-mab-kt52-199202312523645.jpg "Western Blot: EB2 Antibody (KT52) [NBP3-23289] - Western blot of EB proteins in mouse brain (lanes 1-5). The blot was probed with rat monoclonals anti-EB1 (lane 1), anti-EB1/2/3 (lane 2), NBP3-23289 anti-EB2 (lane 3), NBP3-23293 anti-EB3 (lane 4), and NBP3-23294 anti-EB3 (lane 5). Then the antibodies were detected using goat anti-Rat IgG Light Chain specific: HRP.")
![Immunocytochemistry/Immunofluorescence: EB2 Antibody (KT52) [NBP3-23289] - Immunocytochemical labeling of EB2 in paraformaldehyde-fixed and NP40-permeabilized A431 cells. The cells were dual labeled with mouse monoclonal anti-alpha Tubulin (NBP3-23258) (left) and rat monoclonal anti-EB2 (NBP3-23289) (right). The antibodies were detected using either goat anti-mouse: DyLight(R) 488 or goat anti-Rat: DyLight(R) 594.](http://images.novusbio.com/fullsize/nbp3-23289_rat-eb2-mab-kt52-19920231856947.jpg)
![Western Blot: EB3 Antibody (KT36) [NBP3-23294] - Western blot of EB proteins in mouse brain (lanes 1-5). The blot was probed with rat monoclonals anti-EB1 (lane 1), anti-EB1/2/3 (lane 2), NBP3-23289 anti-EB2 (lane 3), NBP3-23293 anti-EB3 (lane 4), and NBP3-23294 anti-EB3 (lane 5). Then the antibodies were detected using goat anti-Rat IgG Light Chain specific: HRP.](http://images.novusbio.com/fullsize/nbp3-23294_rat-eb3-mab-kt36-11920231633169.jpg "Western Blot: EB3 Antibody (KT36) [NBP3-23294] - Western blot of EB proteins in mouse brain (lanes 1-5). The blot was probed with rat monoclonals anti-EB1 (lane 1), anti-EB1/2/3 (lane 2), NBP3-23289 anti-EB2 (lane 3), NBP3-23293 anti-EB3 (lane 4), and NBP3-23294 anti-EB3 (lane 5). Then the antibodies were detected using goat anti-Rat IgG Light Chain specific: HRP.")
![Immunocytochemistry/Immunofluorescence: EB3 Antibody (KT36) [NBP3-23294] - Immunocytochemical labeling of EB3 in paraformaldehyde-fixed and NP40-permeabilized A431 cells. The cells were dual labeled with mouse monoclonal anti-alpha Tubulin (NBP3-23258) (green) and rat monoclonal anti-EB3 (NBP3-23294) (red). The antibodies were detected using either goat anti-mouse: DyLight(R) 488 or goat anti-Rat:DyLight(R) 594.](http://images.novusbio.com/fullsize/nbp3-23294_rat-eb3-mab-kt36-19920231856937.jpg)
![Immunocytochemistry/Immunofluorescence: CD81 Antibody (1.3.3.22) - Chimeric [NBP3-12080] - Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of 1.3.3.22 (NBP3-12080) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-12080, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/CD81-Antibody-1-3-3-22-Chimeric-Immunocytochemistry-Immunofluorescence-NBP3-12080-img0001.jpg "Immunocytochemistry/Immunofluorescence: CD81 Antibody (1.3.3.22) - Chimeric [NBP3-12080] - Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of 1.3.3.22 (NBP3-12080) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-12080, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.")
![Flow Cytometry: CD81 Antibody (1.3.3.22) - Chimeric - Azide and BSA Free [NBP3-12080] - Paraformaldehyde fixed Daudi cells were stained with anti-unknown specificity antibody (isotype control, black line) or NBP3-12080 (blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/nbp3-12080_rabbit-monoclonal-cd81-antibody-1-3-3-22-chimeric-74202522141241.png)
![Western Blot: Septin-5 Antibody (SP18) [NBP2-81058] - Western Blot using [NBP2-81058]. Rat brain (lane 1), mouse spinal cord (lane 2), rat testis (lane 3), human lung cancer (lane 4) and human breast cancer (lane 5) samples were resolved on a 12% SDS PAGE gel and blots probed with NBP2-81058 at 2 ug/ml before being detected by a secondary antibody. Bands correspondig to the expected 43 kDa CDCrel-1 protein were detected in rat brain and mouse spinal chord. In this experiment no CDCrel-1 was dected in the other samples.](http://images.novusbio.com/fullsize/Septin-5-Antibody-SP18-Western-Blot-NBP2-81058-img0001.jpg "Western Blot: Septin-5 Antibody (SP18) [NBP2-81058] - Western Blot using [NBP2-81058]. Rat brain (lane 1), mouse spinal cord (lane 2), rat testis (lane 3), human lung cancer (lane 4) and human breast cancer (lane 5) samples were resolved on a 12% SDS PAGE gel and blots probed with NBP2-81058 at 2 ug/ml before being detected by a secondary antibody. Bands correspondig to the expected 43 kDa CDCrel-1 protein were detected in rat brain and mouse spinal chord. In this experiment no CDCrel-1 was dected in the other samples.")
![Immunohistochemistry: Septin-5 Antibody (SP18) [NBP2-81058] - Immunohistochemical staining of rabbit brain using [NBP2-81058]. Formalin fixed rabbit brain slices were were stained with NBP2-81058 at 3 ug/ml.](http://images.novusbio.com/fullsize/Septin-5-Antibody-SP18-Immunohistochemistry-NBP2-81058-img0002.jpg)
![Flow Cytometry: PRMT5 Antibody (RAB-C136) - Chimeric - Azide and BSA Free [NBP3-20131] - HeLa cells were fixed using 2% PFA and stained with anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG1 version of RAB-C136 (NBP3-20131, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/nbp3-20131_rabbit-prmt5-mab-rab-c136-chimeric-azide-and-bsa-free-2742023924283.png "Flow Cytometry: PRMT5 Antibody (RAB-C136) - Chimeric - Azide and BSA Free [NBP3-20131] - HeLa cells were fixed using 2% PFA and stained with anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG1 version of RAB-C136 (NBP3-20131, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.")
![Immunocytochemistry/Immunofluorescence: PRMT5 Antibody (RAB-C136) - Chimeric - Azide and BSA Free [NBP3-20131] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of RAB-C136 (NBP3-20131) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-20131, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp3-20131_rabbit-prmt5-mab-rab-c136-chimeric-azide-and-bsa-free-2742023924232.png)
![Western Blot: CLASP2 Antibody (KT68) [NBP3-23220] - Western blot of CLASP2 in mouse brain (lanes 1 & 3) and rat PC12 cells (lanes 2 & 4). The blots were probed with rat monoclonals NBP3-23220 anti-CLASP2 (clone KT68) (lanes 1 & 2) and anti-CLASP2 (clone KT69) (lanes 3 & 4). The antibodies were used at a dilution of 1:500.](http://images.novusbio.com/fullsize/nbp3-23220_rat-clasp2-mab-kt68-18920231735526.jpg "Western Blot: CLASP2 Antibody (KT68) [NBP3-23220] - Western blot of CLASP2 in mouse brain (lanes 1 & 3) and rat PC12 cells (lanes 2 & 4). The blots were probed with rat monoclonals NBP3-23220 anti-CLASP2 (clone KT68) (lanes 1 & 2) and anti-CLASP2 (clone KT69) (lanes 3 & 4). The antibodies were used at a dilution of 1:500.")
![Immunocytochemistry/Immunofluorescence: CLASP2 Antibody (KT68) [NBP3-23220] - Immunocytochemical labeling of CLASP2 in paraformaldehyde-fixed and NP40-permeabilized A431 cells. The cells were dual labeled with mouse monoclonal anti-alpha Tubulin (NBP3-23258) (left) and rat monoclonal anti-CLASP2 (NBP3-23220) (middle). The antibodies were detected using either goat anti-mouse: DyLight(R) 488 or goat anti-Rat: DyLight(R) 594.](http://images.novusbio.com/fullsize/nbp3-23220_rat-clasp2-mab-kt68-199202312523627.jpg)