| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47781-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-44406 | |||
| Azide and BSA Free | NBP2-34552-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47897-0.1mg | |||
| IHC-Prediluted | NBP2-48384 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | MAB18384-SP | |||
![Flow Cytometry: Cyclin B1 Antibody (V152) [NBP3-07017] - Separation of JURKAT cells stained using anti-Cyclin B1 (V152) purified antibody (concentration in sample 5.0 ug/mL, GAM APC, red-filled) from JURKAT cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (intracellular staining).](http://images.novusbio.com/fullsize/Cyclin-B1-Antibody-V152-Flow-Cytometry-NBP3-07017-img0001.jpg "Flow Cytometry: Cyclin B1 Antibody (V152) [NBP3-07017] - Separation of JURKAT cells stained using anti-Cyclin B1 (V152) purified antibody (concentration in sample 5.0 ug/mL, GAM APC, red-filled) from JURKAT cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (intracellular staining).")
![Western Blot: E2F-4 Antibody (JE40-78) [NBP3-32285] - Western blot analysis of E2F-4 on different lysates with Rabbit anti-E2F-4 antibody (NBP3-32285) at 1/1,000 dilution. Lane 1: K-562 cell lysate Lane 2: A549 cell lysate Lane 3: HEK-293 cell lysate Lane 4: Jurkat cell lysate Lane 5: HepG2 cell lysate Lane 6: Raji cell lysate Lane 7: HeLa cell lysate Lysates/proteins at 15 ug/Lane. Predicted band size: 44 kDa Observed band size: 60 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32285) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32285_rabbit-e2f-4-mab-je40-78-278202416331292.jpg "Western Blot: E2F-4 Antibody (JE40-78) [NBP3-32285] - Western blot analysis of E2F-4 on different lysates with Rabbit anti-E2F-4 antibody (NBP3-32285) at 1/1,000 dilution. Lane 1: K-562 cell lysate Lane 2: A549 cell lysate Lane 3: HEK-293 cell lysate Lane 4: Jurkat cell lysate Lane 5: HepG2 cell lysate Lane 6: Raji cell lysate Lane 7: HeLa cell lysate Lysates/proteins at 15 ug/Lane. Predicted band size: 44 kDa Observed band size: 60 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32285) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: E2F-4 Antibody (JE40-78) [NBP3-32285] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-E2F-4 antibody (NBP3-32285) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32285) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32285_rabbit-e2f-4-mab-je40-78-298202414304159.jpg)
![Flow Cytometry: E2F-4 Antibody (JE40-78) [NBP3-32285] - Flow cytometric analysis of K-562 cells labeling E2F-4. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32285, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32285_rabbit-e2f-4-mab-je40-78-29202416372181.jpg)
![Immunohistochemistry-Paraffin: p21/CIP1/CDKN1A Antibody (DCS-60.2) - IHC-Prediluted [NBP2-48351] - Formalin-fixed, paraffin-embedded human Bladder Carcinoma stained with p21 Mouse Monoclonal Antibody (DCS-60.2).](http://images.novusbio.com/fullsize/p21-CIP1-CDKN1A-Antibody-DCS-60-2-IHC-Prediluted-Immunohistochemistry-Paraffin-NBP2-48351-img0003.jpg "Immunohistochemistry-Paraffin: p21/CIP1/CDKN1A Antibody (DCS-60.2) - IHC-Prediluted [NBP2-48351] - Formalin-fixed, paraffin-embedded human Bladder Carcinoma stained with p21 Mouse Monoclonal Antibody (DCS-60.2).")
![Western Blot: MCM3 Antibody [NB100-249] - Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified goat anti-MCM3 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/MCM3-Antibody-Western-Blot-NB100-249-img0059.jpg "<b>Biological Strategies Validation. </b>Western Blot: MCM3 Antibody [NB100-249] - Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified goat anti-MCM3 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry: MCM3 Antibody [NB100-249] - Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified goat anti-MCM3 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/MCM3-Antibody-Immunohistochemistry-NB100-249-img0061.jpg)
![Western Blot: MCM3 Antibody [NB100-249] - Whole cell lysate (60 ug) from HeLa cells. Antibody used at 0.25 ug/ml.](http://images.novusbio.com/fullsize/MCM3-Antibody-Western-Blot-NB100-249-img0002.jpg)
![Western Blot: MCM6 Antibody [NB100-251] - Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified goat anti-MCM6 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/MCM6-Antibody-Western-Blot-NB100-251-img0066.jpg "<b>Biological Strategies Validation. </b>Western Blot: MCM6 Antibody [NB100-251] - Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified goat anti-MCM6 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry: MCM6 Antibody [NB100-251] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified goat anti-MCM6 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/MCM6-Antibody-Immunohistochemistry-NB100-251-img0068.jpg)
![Western Blot: MCM6 Antibody [NB100-251] - Whole cell lysate (60 ug) from HeLa cells. Antibody used at 0.25 ug/ml.](http://images.novusbio.com/fullsize/MCM6-Antibody-Western-Blot-NB100-251-img0002.jpg)
![Immunohistochemistry-Paraffin: Cdc20 Antibody (AR12) [NBP2-32954] - Formalin-fixed, paraffin-embedded human Tonsil stained with Cdc20 Antibody (AR12).](http://images.novusbio.com/fullsize/Cdc20-Antibody-AR12-Immunohistochemistry-Paraffin-NBP2-32954-img0003.jpg "Immunohistochemistry-Paraffin: Cdc20 Antibody (AR12) [NBP2-32954] - Formalin-fixed, paraffin-embedded human Tonsil stained with Cdc20 Antibody (AR12).")
![Immunohistochemistry-Paraffin: Cdc20 Antibody (AR12) [NBP2-32954] - Formalin-fixed, paraffin-embedded human Gastric Carcinoma stained with Cdc20 Antibody (AR12).](http://images.novusbio.com/fullsize/Cdc20-Antibody-AR12-Immunohistochemistry-Paraffin-NBP2-32954-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: UbcH3/Cdc34 Antibody [NBP1-32153] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CDC34 protein stained by CDC34 antibody [N1C3] diluted at 1:200. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] diluted at 1:10000. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/UbcH3-Cdc34-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32153-img0007.jpg "Immunocytochemistry/Immunofluorescence: UbcH3/Cdc34 Antibody [NBP1-32153] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CDC34 protein stained by CDC34 antibody [N1C3] diluted at 1:200. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] diluted at 1:10000. Blue: Hoechst 33342 staining.")
![Immunocytochemistry/Immunofluorescence: UbcH3/Cdc34 Antibody [NBP1-32153] - Paraformaldehyde-fixed A549, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/UbcH3-Cdc34-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32153-img0004.jpg)
![UbcH3/Cdc34 Antibody [NBP1-32153] -Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with CDC34 antibody [N1C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-32153_rabbit-polyclonal-ube2r1-cdc34-antibody-western-blot-152023124356..jpg)
![Western Blot: p21/CIP1/CDKN1A Antibody (CIP1/823 + DCS-60.2) [NBP2-44529] - Analysis of human HeLa cell lysate using p21/CIP1/CDKN1A Antibody (CIP1/823 + DCS-60.2).](http://images.novusbio.com/fullsize/p21-CIP1-CDKN1A-Antibody-CIP1-823-+-DCS-60-2-Western-Blot-NBP2-44529-img0003.jpg "Western Blot: p21/CIP1/CDKN1A Antibody (CIP1/823 + DCS-60.2) [NBP2-44529] - Analysis of human HeLa cell lysate using p21/CIP1/CDKN1A Antibody (CIP1/823 + DCS-60.2).")
![Immunohistochemistry-Paraffin: p21/CIP1/CDKN1A Antibody (CIP1/823 + DCS-60.2) [NBP2-44529] - Human Bladder Carcinoma stained with p21 Monoclonal Antibody (CIP1/823 + DCS-60.2).](http://images.novusbio.com/fullsize/p21-CIP1-CDKN1A-Antibody-CIP1-823-+-DCS-60-2-Immunohistochemistry-Paraffin-NBP2-44529-img0001.jpg)
![Western Blot: CDC45L Antibody [NBP2-15834] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CDC45L antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/CDC45L-Antibody-Western-Blot-NBP2-15834-img0004.jpg "Western Blot: CDC45L Antibody [NBP2-15834] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CDC45L antibody diluted at 1:1000.")
![Western Blot: CDC45L Antibody [NBP2-15834] - Sample (30 ug of whole cell lysate) A: HeLa 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/CDC45L-Antibody-Western-Blot-NBP2-15834-img0001.jpg)
![CDC45L antibody [N3C3] detects CDC45L protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: CDC45L protein stained by CDC45L antibody [N3C3] (NBP2-15834) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] diluted at 1:10000.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp2-15834_rabbit-polyclonal-cdc45l-antibody-immunocytochemistry-immunofluorescence-261120251826136.jpg)
![Western Blot: E2F-1 [p Ser364] Antibody [NB600-900] - Panel A: Control antibody reactive to all forms of E2F (arrowheads). Panel B: Reactivity with phosphorylated E2F-1 (arrows) using E2F-1 [p Ser364] antibody. Lysate cells: CRE/E2F-1 CRE (human E2F-1 transfected mouse NIH3T3); NIH-3T3 (-ve control), & MDA-MB-231. Each lysate prepared from untreated cells & cells with 2 uM Doxorubicin. MDA-MB-231 also treated with genistein. Same membrane probed with E2F-1 [p Ser364] antibody @ 1:250 dilution, then stripped & re-probed with pan E2F antibody (+ve control). +ve control antibody shows an E2F-1 band in all human cell lines, but not mouse cells. Doxorubicin increases expression of E2F-1 (Panel A). Images overlapped to confirm that specific anti-E2F-1 pS364 staining shown in treated human cells in Panel B specifically aligns with E2F-1 staining shown in Panel A. Blots can be processed with HRP conjugated Gt-a-Rabbit IgG MX10 for 45 min at RT for ECL detection.](http://images.novusbio.com/fullsize/E2F-1-[p-Ser364]-Antibody-Western-Blot-NB600-900-img0007.jpg "Western Blot: E2F-1 [p Ser364] Antibody [NB600-900] - Panel A: Control antibody reactive to all forms of E2F (arrowheads). Panel B: Reactivity with phosphorylated E2F-1 (arrows) using E2F-1 [p Ser364] antibody. Lysate cells: CRE/E2F-1 CRE (human E2F-1 transfected mouse NIH3T3); NIH-3T3 (-ve control), & MDA-MB-231. Each lysate prepared from untreated cells & cells with 2 uM Doxorubicin. MDA-MB-231 also treated with genistein. Same membrane probed with E2F-1 [p Ser364] antibody @ 1:250 dilution, then stripped & re-probed with pan E2F antibody (+ve control). +ve control antibody shows an E2F-1 band in all human cell lines, but not mouse cells. Doxorubicin increases expression of E2F-1 (Panel A). Images overlapped to confirm that specific anti-E2F-1 pS364 staining shown in treated human cells in Panel B specifically aligns with E2F-1 staining shown in Panel A. Blots can be processed with HRP conjugated Gt-a-Rabbit IgG MX10 for 45 min at RT for ECL detection.")
![Immunohistochemistry: E2F-1 [p Ser364] Antibody [NB600-900] - Affinity Purified E2F-1 [p Ser364] antibody was used at a 10 ug/ml to detect nuclear and occasionally cytoplasmic signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. Within the multi-tumor block, the antibody showed variable levels of nuclear staining between individual tumors, with some tumors showing strong staining. This image shows E2F-1 pS364 staining of human breast carcinoma. Tissue was formalin-fixed and paraffin embedded.](http://images.novusbio.com/fullsize/E2F-1-[p-Ser364]-Antibody-Immunohistochemistry-NB600-900-img0008.jpg)
![Western Blot: E2F-1 [p Ser364] Antibody [NB600-900] - NB600-900 Western blot - E2F1 (phospho S364) antibody](http://images.novusbio.com/fullsize/E2F-1-[p-Ser364]-Antibody-Western-Blot-NB600-900-img0001.jpg)
![Western Blot: Orc2 Antibody [NBP1-46176] - Detection of human ORC2 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-ORC2 antibody NBP1-46176 used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 3 ug/mg lysate. ORC2 was also immunoprecipitated by rabbit anti-ORC2 antibody NBP1-46175, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).](http://images.novusbio.com/fullsize/Orc2-Antibody-Western-Blot-NBP1-46176-img0005.jpg "<b>Independent Antibodies Validation. </b>Western Blot: Orc2 Antibody [NBP1-46176] - Detection of human ORC2 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-ORC2 antibody NBP1-46176 used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 3 ug/mg lysate. ORC2 was also immunoprecipitated by rabbit anti-ORC2 antibody NBP1-46175, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry: Orc2 Antibody [NBP1-46176] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-ORC2 used at a dilution of 1:200 (1ug/ml) Detection: DAB staining using Immunohistochemistry Accessory Kit.](http://images.novusbio.com/fullsize/Orc2-Antibody-Immunohistochemistry-NBP1-46176-img0003.jpg)