| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-27149-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier-Free | NBP2-34897-10ug | |||
| Carrier-Free | NBP2-34897-1mg | |||
| Carrier-Free | NBP2-34897-500ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier-Free | NBP2-35153-1mg | |||
| Carrier-Free | NBP2-35153-100ug | |||
| Carrier-Free | NBP2-35153-500ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-52682-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-20107-0.025mg | |||
![Immunohistochemistry: CD30/TNFRSF8 Antibody (PSH04-12) [NBP3-32144] - Immunohistochemical analysis of paraffin-embedded human anaplastic large cell lymphoma tissue with Rabbit anti-CD30/TNFRSF8 antibody (NBP3-32144) at 1/1,500 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32144) at 1/1,500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32144_rabbit-cd30-tnfrsf8-mab-psh04-12-29820241492678.jpg "Immunohistochemistry: CD30/TNFRSF8 Antibody (PSH04-12) [NBP3-32144] - Immunohistochemical analysis of paraffin-embedded human anaplastic large cell lymphoma tissue with Rabbit anti-CD30/TNFRSF8 antibody (NBP3-32144) at 1/1,500 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32144) at 1/1,500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunocytochemistry/ Immunofluorescence: CD30/TNFRSF8 Antibody (PSH04-12) [NBP3-32144] - Immunocytochemistry analysis of HDLM-2 (positive) and HeLa (negative) labeling CD30/TNFRSF8 with Rabbit anti-CD30/TNFRSF8 antibody (NBP3-32144) at 1/100 dilution.<br/><br/> Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CD30/TNFRSF8 antibody (NBP3-32144) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br/><br/> Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32144_rabbit-cd30-tnfrsf8-mab-psh04-12-2920241192361.jpg)
![Western blot analysis on different lysates with NBP3-32144 at 1/1,000 dilution. Lane 1: HDLM-2 cell lysate Lane 2: HUT 102 cell lysate Lane 3: U-937 cell lysate (negative) Lane 4: HL-60 cell lysate (negative) Lane 5: Daudi cell lysate (negative) Lane 6: HeLa cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 64 kDa Observed band size: 75-120 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32144 at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32144_-western-blot-639204597485294942.jpg)
![Western Blot: Flightless I Antibody [NBP3-32341] - Western blot analysis of Flightless I on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32341, 1/500) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.<br/> Positive control: <br/> Lane 1: Mouse lung tissue lysate<br/> Lane 2: MCF-7 cell lysate](http://images.novusbio.com/fullsize/nbp3-32341_rabbit-flightless-i-pab-2782024163164.jpg "Western Blot: Flightless I Antibody [NBP3-32341] - Western blot analysis of Flightless I on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32341, 1/500) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.<br/> Positive control: <br/> Lane 1: Mouse lung tissue lysate<br/> Lane 2: MCF-7 cell lysate")
![Immunohistochemistry: Flightless I Antibody [NBP3-32341] - Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-Flightless I antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32341, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32341_rabbit-flightless-i-pab-298202414115421.jpg)
![Western blot analysis on different lysates with NBP3-32341 at 1/10,000 dilution. Lane 1: HeLa (Human cervical adenocarcinoma cell) cell lysate Lane 2: A431 (Human epidermoid carcinoma skin squamous cell) cell lysate Lane 3: A549 (Human lung adenocarcinoma cell) cell lysate Lane 4: MCF7 (Human breast cancer cell) cell lysate Lysates/proteins at 15 µg/Lane. Exposure time: 20 seconds Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: NBP3-32341, 1/10,000 in primary antibody dilution buffer, overnight at 4 ℃ Secondary antibody: Goat Anti-Rabbit IgG [HRP] Secondary Antibody, 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 145 kDa Observed band size: 145 kDa](http://images.novusbio.com/fullsize/nbp3-32341_-western-blot-639204590071891109.jpg)
![SDS-Page: Recombinant Human Lamin A Protein [H00004000-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00004000-P01-1.jpg "SDS-Page: Recombinant Human Lamin A Protein [H00004000-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Western Blot: TOB1 Antibody [H00010140-B01P] - Analysis of TOB1 expression in transfected 293T cell line by TOB1 polyclonal antibody. Lane 1: TOB1 transfected lysate(37.95 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/TOB1-Antibody-Western-Blot-H00010140-B01P-img0006.jpg "Western Blot: TOB1 Antibody [H00010140-B01P] - Analysis of TOB1 expression in transfected 293T cell line by TOB1 polyclonal antibody. Lane 1: TOB1 transfected lysate(37.95 KDa). Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: TOB1 Antibody [H00010140-B01P] - Analysis of purified antibody to TOB1 on HeLa cell. (antibody concentration 10 ug/ml)](http://images.novusbio.com/fullsize/TOB1-Antibody-Immunocytochemistry-Immunofluorescence-H00010140-B01P-img0004.jpg)
![Immunohistochemistry-Paraffin: TOB1 Antibody [H00010140-B01P] - Analysis of purified antibody to TOB1 on formalin-fixed paraffin-embedded human endometrium. (antibody concentration 3 ug/ml)](http://images.novusbio.com/fullsize/TOB1-Antibody-Immunohistochemistry-Paraffin-H00010140-B01P-img0005.jpg)
![Western Blot: SGTA Antibody [H00006449-B01P] - Analysis of SGTA expression in human liver.](http://images.novusbio.com/fullsize/SGTA-Antibody-Western-Blot-H00006449-B01P-img0006.jpg "Western Blot: SGTA Antibody [H00006449-B01P] - Analysis of SGTA expression in human liver.")
![Immunocytochemistry/Immunofluorescence: SGTA Antibody [H00006449-B01P] - Analysis of purified antibody to SGTA on HeLa cell. (antibody concentration 10 ug/ml)](http://images.novusbio.com/fullsize/SGTA-Antibody-Immunocytochemistry-Immunofluorescence-H00006449-B01P-img0004.jpg)
![Western Blot: SGTA Antibody [H00006449-B01P] - Analysis of SGTA expression in Jurkat.](http://images.novusbio.com/fullsize/SGTA-Antibody-Western-Blot-H00006449-B01P-img0005.jpg)
![Western Blot: SGTA Antibody [H00006449-B01P] - Analysis of SGTA expression in transfected 293T cell line by SGTA polyclonal antibody. Lane 1: SGTA transfected lysate(34.43 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/SGTA-Antibody-Western-Blot-H00006449-B01P-img0007.jpg)
![Western Blot: Septin-3 Antibody (4D8) [H00055964-M03] - Analysis of SEPT3 expression in Raw 264.7 (Cat # L024V1).](http://images.novusbio.com/fullsize/Septin-3-Antibody-4D8-Western-Blot-H00055964-M03-img0006.jpg "Western Blot: Septin-3 Antibody (4D8) [H00055964-M03] - Analysis of SEPT3 expression in Raw 264.7 (Cat # L024V1).")
![Western Blot: Septin-3 Antibody (4D8) [H00055964-M03] - Analysis of SEPT3 expression in PC-12 (Cat # L012V1).](http://images.novusbio.com/fullsize/Septin-3-Antibody-4D8-Western-Blot-H00055964-M03-img0005.jpg)
![Western Blot: Septin-3 Antibody (4D8) [H00055964-M03] - Analysis of SEPT3 expression in Jurkat (Cat # L017V1).](http://images.novusbio.com/fullsize/Septin-3-Antibody-4D8-Western-Blot-H00055964-M03-img0007.jpg)
![ELISA: Septin-3 Antibody (4D8) [H00055964-M03] - Detection limit for recombinant GST tagged 3-Sep is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Septin-3-Antibody-4D8-ELISA-H00055964-M03-img0004.jpg)
![Western Blot: HBO1 Antibody (4E12H12) [NBP3-27149] - Analysis using HBO1 mAb against HEK293 (1) and HBO1 (AA: 1-200)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/nbp3-27149_mouse-hbo1-mab-4e12h12-bsa-free-10420249141932.jpg%3Fv=1666704272 "Western Blot: HBO1 Antibody (4E12H12) [NBP3-27149] - Analysis using HBO1 mAb against HEK293 (1) and HBO1 (AA: 1-200)-hIgGFc transfected HEK293 (2) cell lysate.")
![ELISA: HBO1 Antibody (4E12H12) [NBP3-27149] - Black line: Control Antigen (100 ng); Purple line: Antigen (10 ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)](http://images.novusbio.com/fullsize/nbp3-27149_mouse-hbo1-mab-4e12h12-bsa-free-1042024915839.jpg%3Fv=1666704272)
![Western Blot: HBO1 Antibody (4E12H12) [NBP3-27149] - Analysis using HBO1 mAb against human HBO1 (AA: 1-200) recombinant protein. (Expected MW is 48.1 kDa)](http://images.novusbio.com/fullsize/nbp3-27149_mouse-hbo1-mab-4e12h12-bsa-free-1042024915849.jpg%3Fv=1666704272)
![Western Blot: HBO1 Antibody (4E12H12) [NBP3-27149] - Analysis using HBO1 mouse mAb against MOLT4 (1), COS7 (2), F9 (3), HepG2 (4), PC-2 (5), U251 (6), MCF-7 (7), and NIH/3T3 (8) cell lysate.](http://images.novusbio.com/fullsize/nbp3-27149_mouse-hbo1-mab-4e12h12-bsa-free-1042024920235.jpg%3Fv=1666704272)
![SDS-Page: Human IL-5 Protein [NBP2-34897]](http://images.novusbio.com/fullsize/Human-IL-5-Protein-SDS-Page-NBP2-34897-img0002.jpg "SDS-Page: Human IL-5 Protein [NBP2-34897]")
![SDS-Page: Mouse LIF Protein [NBP2-35153]](http://images.novusbio.com/fullsize/Mouse-LIF-Protein-SDS-Page-NBP2-35153-img0001.jpg "SDS-Page: Mouse LIF Protein [NBP2-35153]")
![Immunohistochemistry: CD30/TNFRSF8 Antibody (Ki-3) - Chimeric [NBP2-52682] - Immunohistochemical staining of human tonsil tissue using anti-CD30 antibody. Ki-3 Anti-CD30 staining of paraffin embedded human tonsil tissue using the rabbit-chimeric version of Ki-3 (NBP2-52682). Antigen retreival was acheived by microwaving in citrate buffer, followed by blocking with protein block serum-free buffer. Primary antibody incubation with NBP2-52682 was carried out at 4 ug/ml for 30 minutes. Samples were then incubated with an anti-rabbit IgG HRP secondary antibody for 20 mins followed by DAB (3,3'-diaminobenzidine), and counter-staining with haemotoxylin. Staining of the cellular membrane of dividing cells may be observed. Recommended concentration, 2-4 ug/ml.](http://images.novusbio.com/fullsize/CD30-TNFRSF8-Antibody-Ki-3-Chimeric-Immunohistochemistry-NBP2-52682-img0001.jpg "Immunohistochemistry: CD30/TNFRSF8 Antibody (Ki-3) - Chimeric [NBP2-52682] - Immunohistochemical staining of human tonsil tissue using anti-CD30 antibody. Ki-3 Anti-CD30 staining of paraffin embedded human tonsil tissue using the rabbit-chimeric version of Ki-3 (NBP2-52682). Antigen retreival was acheived by microwaving in citrate buffer, followed by blocking with protein block serum-free buffer. Primary antibody incubation with NBP2-52682 was carried out at 4 ug/ml for 30 minutes. Samples were then incubated with an anti-rabbit IgG HRP secondary antibody for 20 mins followed by DAB (3,3'-diaminobenzidine), and counter-staining with haemotoxylin. Staining of the cellular membrane of dividing cells may be observed. Recommended concentration, 2-4 ug/ml.")
![Flow Cytometry: CD30/TNFRSF8 Antibody (Ki-3) - Chimeric [NBP2-52682] - Flow-cytometry using the anti-CD30 (Tumor necrosis factor receptor superfamily member 8) antibody Ki-3. Jurkat cells were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of Ki-3 (NBP2-52682, blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using an anti-rabbit IgG JK (FITC-conjugate) antibody at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/CD30-TNFRSF8-Antibody-Ki-3-Chimeric-Flow-Cytometry-NBP2-52682-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: p38 alpha Antibody (M138) [NBP3-23233] - Immunocytochemical labeling of activated p38 MAPK in pervanadate-treated mouse C2C12. The cells were labeled with mouse monoclonal p38 alpha MAPK and p38 MAPK (T180/Y182) antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3.](http://images.novusbio.com/fullsize/nbp3-23233_mouse-p38-alpha-mab-m138-19920231410114.jpg "<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: p38 alpha Antibody (M138) [NBP3-23233] - Immunocytochemical labeling of activated p38 MAPK in pervanadate-treated mouse C2C12. The cells were labeled with mouse monoclonal p38 alpha MAPK and p38 MAPK (T180/Y182) antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3.")
![<b>Biological Strategies Validation. </b>Western Blot: p38 alpha Antibody (M138) [NBP3-23233] - Western blot analysis of A431 cells serum starved overnight (lanes 1 & 3) or treated with pervanadate (1 mM) for 30 minutes (lanes 2 & 4). The blot was probed with anti-p38 alpha (lanes 1 & 2) or anti-p38 (T180/Y182) (lanes 3-4). Lanes 5-7 shows a blot of A431 cells treated with pervanadate and probed with anti-p38 (T180/Y182) in the presence of no peptide (lane 5), phospho-ERK1 (T202/Y204) peptide (lane 6) or phospho-p38 (T180/Y182) peptide (lane 7).](http://images.novusbio.com/fullsize/nbp3-23233_mouse-p38-alpha-mab-m138-19920231252360.jpg)
![Western Blot: UbcH10/UBE2C Antibody (SAIC-41A-1) - Azide and BSA Free [NBP3-20107] - Caco-2(A) (0.3ug/ml), MCF7(B) (1ug/ml) and HeLa(C) (1ug/ml) cell lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of SAIC-41A-1 (NBP3-20107) before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.](http://images.novusbio.com/fullsize/nbp3-20107_rabbit-ubch10-ube2c-mab-saic-41a-1-azide-and-bsa-free-2742023924254.png "Western Blot: UbcH10/UBE2C Antibody (SAIC-41A-1) - Azide and BSA Free [NBP3-20107] - Caco-2(A) (0.3ug/ml), MCF7(B) (1ug/ml) and HeLa(C) (1ug/ml) cell lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of SAIC-41A-1 (NBP3-20107) before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.")
![Flow Cytometry: UbcH10/UBE2C Antibody (SAIC-41A-1) - Azide and BSA Free [NBP3-20107] - Paraformaldehyde fixed MCF7 cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG version of SAIC-41A-1 (NBP3-20107, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/nbp3-20107_rabbit-ubch10-ube2c-mab-saic-41a-1-azide-and-bsa-free-2742023964610.png)
![Immunocytochemistry/Immunofluorescence: UbcH10/UBE2C Antibody (SAIC-41A-1) - Azide and BSA Free [NBP3-20107] - Immunofluorescence analysis of paraformaldehyde fixed Caco-2 cells permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of SAIC-41A-1 (NBP3-20107) (1:100 dilution) for 1h followed by Alexa Fluor(R) 488 secondary antibody (1:1000 dilution), showing NUCLEAR staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-20107, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp3-20107_rabbit-ubch10-ube2c-mab-saic-41a-1-azide-and-bsa-free-2742023924231.png)