| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-33954-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-84620-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-91774-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-90196-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-46693-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-14016 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52436-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-25152SS | |||
![Immunohistochemistry-Paraffin: MCM2 Antibody [NBP2-33954] - Staining in human lymph node and kidney tissues using anti-MCM2 antibody. Corresponding MCM2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/MCM2-Antibody-Immunohistochemistry-Paraffin-NBP2-33954-img0010.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: MCM2 Antibody [NBP2-33954] - Staining in human lymph node and kidney tissues using anti-MCM2 antibody. Corresponding MCM2 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: MCM2 Antibody [NBP2-33954] - Analysis in human cell line HEL.](http://images.novusbio.com/fullsize/MCM2-Antibody-Western-Blot-NBP2-33954-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: MCM2 Antibody [NBP2-33954] - Staining of human cell line MCF7 shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/MCM2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-33954-img0011.jpg)
![Immunohistochemistry-Paraffin: MCM2 Antibody [NBP2-33954] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/MCM2-Antibody-Immunohistochemistry-Paraffin-NBP2-33954-img0016.jpg)
![Immunohistochemistry-Paraffin: MCM2 Antibody [NBP2-33954] - Staining of human lymph node shows high expression.](http://images.novusbio.com/fullsize/MCM2-Antibody-Immunohistochemistry-Paraffin-NBP2-33954-img0009.jpg)
![Immunohistochemistry-Paraffin: MCM2 Antibody [NBP2-33954] - Staining of human kidney shows no positivity in cells in tubules as expected.](http://images.novusbio.com/fullsize/MCM2-Antibody-Immunohistochemistry-Paraffin-NBP2-33954-img0014.jpg)
![Immunohistochemistry-Paraffin: MCM2 Antibody [NBP2-33954] - Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/MCM2-Antibody-Immunohistochemistry-Paraffin-NBP2-33954-img0017.jpg)
![Immunocytochemistry/Immunofluorescence: ELAC2 Antibody [NBP1-84620] - Staining of human cell line A-431 shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/ELAC2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-84620-img0009.jpg "Immunocytochemistry/Immunofluorescence: ELAC2 Antibody [NBP1-84620] - Staining of human cell line A-431 shows localization to nucleoplasm. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: ELAC2 Antibody [NBP1-84620] - Staining of human testis shows strong nuclear and nucleolar positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/ELAC2-Antibody-Immunohistochemistry-Paraffin-NBP1-84620-img0013.jpg)
![Immunohistochemistry-Paraffin: ELAC2 Antibody [NBP1-84620] - Staining of human fallopian tube shows weak to moderate nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/ELAC2-Antibody-Immunohistochemistry-Paraffin-NBP1-84620-img0010.jpg)
![Immunohistochemistry-Paraffin: ELAC2 Antibody [NBP1-84620] - Staining of human lymph node shows moderate to strong nuclear positivity in germinal center cells.](http://images.novusbio.com/fullsize/ELAC2-Antibody-Immunohistochemistry-Paraffin-NBP1-84620-img0011.jpg)
![Immunohistochemistry-Paraffin: ELAC2 Antibody [NBP1-84620] - Staining of human skin shows strong positivity in nucleoli in squamous epithelial cells.](http://images.novusbio.com/fullsize/ELAC2-Antibody-Immunohistochemistry-Paraffin-NBP1-84620-img0012.jpg)
![Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/nbp1-84620_rabbit-polyclonal-elac2-antibody-104202515403158.jpg)
![Immunocytochemistry/Immunofluorescence: FZR1/CDH1 Antibody [NBP1-91774] - Staining of human cell line A-431 shows localization to nucleoplasm & nuclear membrane. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/FZR1-CDH1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-91774-img0004.jpg "Immunocytochemistry/Immunofluorescence: FZR1/CDH1 Antibody [NBP1-91774] - Staining of human cell line A-431 shows localization to nucleoplasm & nuclear membrane. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: FZR1/CDH1 Antibody [NBP1-91774] - Staining of human liver shows strong cytoplasmic and nuclear positivity in hepatocytes.](http://images.novusbio.com/fullsize/FZR1-CDH1-Antibody-Immunohistochemistry-Paraffin-NBP1-91774-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: AKAP95/AKAP8 Antibody [NBP1-90196] - Staining of human cell line U-251 MG shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/AKAP95-AKAP8-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-90196-img0006.jpg "Immunocytochemistry/Immunofluorescence: AKAP95/AKAP8 Antibody [NBP1-90196] - Staining of human cell line U-251 MG shows localization to nucleoplasm.")
![Western Blot: AKAP95/AKAP8 Antibody [NBP1-90196] - Lane 1: Marker [kDa] 229, 112, 84, 48, 32, 27, 17. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/AKAP95-AKAP8-Antibody-Western-Blot-NBP1-90196-img0005.jpg)
![Western Blot: MCM2 Antibody (BLR108H) [NBP3-07021] - Detection of human Phospho-MCM2 by WB of immunoprecipitates from HeLa lysate. Antibodies: Rabbit anti-MCM2 and Phospho-MCM2. Lower Panel: Total MCM2.](http://images.novusbio.com/fullsize/MCM2-Antibody-BLR108H-Western-Blot-NBP3-07021-img0003.jpg "Western Blot: MCM2 Antibody (BLR108H) [NBP3-07021] - Detection of human Phospho-MCM2 by WB of immunoprecipitates from HeLa lysate. Antibodies: Rabbit anti-MCM2 and Phospho-MCM2. Lower Panel: Total MCM2.")
![Immunocytochemistry: MCM2 Antibody (BLR108H) [NBP3-07021] - Detection of human Phospho-MCM2 (S53) in FFPE OVCAR-4 cells by ICC. Antibody: Rabbit anti-MCM2 (S53) recombinant monoclonal [BLR108H]. Secondary: HRP-conjugated goat anti-rabbit IgG Substrate: DAB.](http://images.novusbio.com/fullsize/MCM2-Antibody-BLR108H-Immunocytochemistry-NBP3-07021-img0006.jpg)
![Immunohistochemistry: MCM2 Antibody (BLR108H) [NBP3-07021] - Detection of mouse Phospho-MCM2 (S53) in FFPE mouse renal cell carcinoma by IHC. Antibody: Rabbit anti-MCM2 (S53) recombinant monoclonal [BLR108H]. Secondary: HRP-conjugated goat anti-rabbit IgG Substrate: DAB.](http://images.novusbio.com/fullsize/MCM2-Antibody-BLR108H-Immunohistochemistry-NBP3-07021-img0005.jpg)
![Western Blot: MCM2 Antibody (BLR108H) [NBP3-07021] - Detection of human Phospho-MCM2 by WB of HDLM-2, HeLa, Jurkat, MCF-7, MOLT-4, Malme-3M, HEK293T, and Raji lysate. Antibody: Rabbit anti-Phospho-MCM2 recombinant monoclonal [BLR108H]. Secondary: HRP-conjugated goat anti-rabbit IgG. Lower Panel: Rabbit anti-Actin recombinant monoclonal.](http://images.novusbio.com/fullsize/MCM2-Antibody-BLR108H-Western-Blot-NBP3-07021-img0001.jpg)
![Western Blot: MCM2 Antibody (BLR108H) [NBP3-07021] - Detection of mouse Phospho-MCM2 (S53) by WB of BW5147.3, TCMK-1, mIMCD-3, A20, NIH 3T3, and CT26 lysate. Antibody: Rabbit anti-Phospho-MCM2 (S53) recombinant monoclonal [BLR108H]. Secondary: HRP-conjugated goat anti-rabbit IgG. Lower Panel: Rabbit anti-Actin recombinant monoclonal.](http://images.novusbio.com/fullsize/MCM2-Antibody-BLR108H-Western-Blot-NBP3-07021-img0002.jpg)
![Immunohistochemistry: MCM2 Antibody (BLR108H) [NBP3-07021] - Detection of human Phospho-MCM2 (S53) in FFPE lung carcinoma by IHC. Antibody: Rabbit anti-MCM2 (S53) recombinant monoclonal [BLR108H]. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB](http://images.novusbio.com/fullsize/MCM2-Antibody-BLR108H-Immunohistochemistry-NBP3-07021-img0004.jpg)
![Western Blot: PP2A alpha Antibody [NBP2-46693] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/fullsize/PP2A-alpha-Antibody-Western-Blot-NBP2-46693-img0005.jpg "Western Blot: PP2A alpha Antibody [NBP2-46693] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).")
![Immunohistochemistry: PP2A alpha Antibody [NBP2-46693] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/PP2A-alpha-Antibody-Immunohistochemistry-NBP2-46693-img0001.jpg)
![Western Blot: PP2A alpha Antibody [NBP2-46693] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10, Lane 2: Human cell line RT-4, Lane 3: Human cell line U-251 MG](http://images.novusbio.com/fullsize/PP2A-alpha-Antibody-Western-Blot-NBP2-46693-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: NFIA Antibody (PCRP-NFIA-2C6) [NBP3-13982] - Immunofluorescence Analysis of PFA-fixed HeLa cells stained using NFIA Antibody (PCRP-NFIA-2C6) followed by goat anti-mouse IgG-CF488 (green). CF640A phalloidin (red).](http://images.novusbio.com/fullsize/NFIA-Antibody-PCRP-NFIA-2C6-Immunocytochemistry-Immunofluorescence-NBP3-13982-img0002.jpg "Immunocytochemistry/Immunofluorescence: NFIA Antibody (PCRP-NFIA-2C6) [NBP3-13982] - Immunofluorescence Analysis of PFA-fixed HeLa cells stained using NFIA Antibody (PCRP-NFIA-2C6) followed by goat anti-mouse IgG-CF488 (green). CF640A phalloidin (red).")
![Immunohistochemistry-Paraffin: NFIA Antibody (PCRP-NFIA-2C6) [NBP3-13982] - Formalin-fixed, paraffin-embedded human colon carcinoma stained with NFIA antibody (PCRP-NFIA-2C6). HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.](http://images.novusbio.com/fullsize/NFIA-Antibody-PCRP-NFIA-2C6-Immunohistochemistry-Paraffin-NBP3-13982-img0004.jpg)
![Flow Cytometry: NFIA Antibody (PCRP-NFIA-2C6) [NBP3-13982] - Flow cytometric analysis of PFA-fixed HeLa cells. NFIA antibody (PCRP-NFIA-2C6) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).](http://images.novusbio.com/fullsize/NFIA-Antibody-PCRP-NFIA-2C6-Flow-Cytometry-NBP3-13982-img0001.jpg)
![Immunohistochemistry-Paraffin: NFIA Antibody (PCRP-NFIA-2C6) [NBP3-13982] - Formalin-fixed, paraffin-embedded human lung stained with NFIA antibody (PCRP-NFIA-2C6). HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.](http://images.novusbio.com/fullsize/NFIA-Antibody-PCRP-NFIA-2C6-Immunohistochemistry-Paraffin-NBP3-13982-img0003.jpg)
![Protein Array: NFIA Antibody (PCRP-NFIA-2C6) [NBP3-13982] - Analysis of Protein Array containing more than 19,000 full-length human proteins using NFIA Antibody (PCRP-NFIA-2C6).](http://images.novusbio.com/fullsize/NFIA-Antibody-PCRP-NFIA-2C6-Protein-Array-NBP3-13982-img0006.jpg)
![Western Blot: RPA2 Antibody (MA34) [NB100-74526] - Analysis of purified RPA protein](http://images.novusbio.com/fullsize/RPA2-Antibody-MA34-Western-Blot-NB100-74526-img0005.jpg "Western Blot: RPA2 Antibody (MA34) [NB100-74526] - Analysis of purified RPA protein")
![Immunocytochemistry/Immunofluorescence: RPA2 Antibody (MA34) [NB100-74526] - Analysis of RPA34 in A431 cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a RPA34 monoclonal antibody at a dilution of 1:200 overnight at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. RPA34 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.](http://images.novusbio.com/fullsize/RPA2-Antibody-MA34-Immunocytochemistry-Immunofluorescence-NB100-74526-img0013.jpg)
![Immunohistochemistry-Paraffin: RPA2 Antibody (MA34) [NB100-74526] - Normal biopsies of deparaffinized Human kidney tissue at a dilution of 1:20.](http://images.novusbio.com/fullsize/RPA2-Antibody-MA34-Immunohistochemistry-Paraffin-NB100-74526-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: RPA2 Antibody (MA34) [NB100-74526] - Analysis of RPA34 in HeLa cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a RPA34 monoclonal antibody at a dilution of 1:200 overnight at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. RPA34 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.](http://images.novusbio.com/fullsize/RPA2-Antibody-MA34-Immunocytochemistry-Immunofluorescence-NB100-74526-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: RPA2 Antibody (MA34) [NB100-74526] - Analysis of RPA34 in C6 cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a RPA34 monoclonal antibody at a dilution of 1:200 overnight at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. RPA34 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.](http://images.novusbio.com/fullsize/RPA2-Antibody-MA34-Immunocytochemistry-Immunofluorescence-NB100-74526-img0012.jpg)
![Immunohistochemistry-Paraffin: RPA2 Antibody (MA34) [NB100-74526] - Cancer biopsies of deparaffinized Human breast carcinoma tissue.](http://images.novusbio.com/fullsize/RPA2-Antibody-MA34-Immunohistochemistry-Paraffin-NB100-74526-img0010.jpg)
![Immunohistochemistry-Paraffin: RPA2 Antibody (MA34) [NB100-74526] - Normal biopsies of deparaffinized Human tonsil tissue at a dilution of 1:20.](http://images.novusbio.com/fullsize/RPA2-Antibody-MA34-Immunohistochemistry-Paraffin-NB100-74526-img0014.jpg)
![Western Blot: Cyclin A2 Antibody (6B4D11) [NBP2-52436] - Analysis using CCNA2 mouse mAb against Hela (1), HEK293 (2), Jurkat (3), K562 (4), SK-Br-3 (5), NIH/3T3 (6) cell lysate.](http://images.novusbio.com/fullsize/Cyclin-A2-Antibody-6B4D11-Western-Blot-NBP2-52436-img0007.jpg "Western Blot: Cyclin A2 Antibody (6B4D11) [NBP2-52436] - Analysis using CCNA2 mouse mAb against Hela (1), HEK293 (2), Jurkat (3), K562 (4), SK-Br-3 (5), NIH/3T3 (6) cell lysate.")
![Immunohistochemistry: Cyclin A2 Antibody (6B4D11) [NBP2-52436] - Analysis of paraffin-embedded bladder cancer tissues using CCNA2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Cyclin-A2-Antibody-6B4D11-Immunohistochemistry-NBP2-52436-img0003.jpg)
![Flow Cytometry: Cyclin A2 Antibody (6B4D11) [NBP2-52436] - 4: Flow cytometric analysis of A431 cells using CCNA2 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Cyclin-A2-Antibody-6B4D11-Flow-Cytometry-NBP2-52436-img0001.jpg)
![Western Blot: Cyclin A2 Antibody (6B4D11) [NBP2-52436] - Analysis using CCNA2 mAb against HEK293 (1) and CCNA2 (AA: 105-233)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Cyclin-A2-Antibody-6B4D11-Western-Blot-NBP2-52436-img0005.jpg)
![Western Blot: Cyclin A2 Antibody (6B4D11) [NBP2-52436] - Analysis using CCNA2 mAb against human CCNA2 (AA: 105-233) recombinant protein. (Expected MW is 40.8 kDa)](http://images.novusbio.com/fullsize/Cyclin-A2-Antibody-6B4D11-Western-Blot-NBP2-52436-img0004.jpg)
![Immunohistochemistry: Cyclin A2 Antibody (6B4D11) [NBP2-52436] - Analysis of paraffin-embedded cervical cancer tissues using CCNA2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Cyclin-A2-Antibody-6B4D11-Immunohistochemistry-NBP2-52436-img0002.jpg)
![ELISA: Cyclin A2 Antibody (6B4D11) [NBP2-52436] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).](http://images.novusbio.com/fullsize/Cyclin-A2-Antibody-6B4D11-ELISA-NBP2-52436-img0006.jpg)
![Western Blot: Lamin A + C Antibody (131C3) [NBP1-97687] - Immunoblotting result of NBP1-97687(131C3) recognizing nuclear lamins A and C in human fibroblasts.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-131C3-Western-Blot-NBP1-97687-img0003.jpg "Western Blot: Lamin A + C Antibody (131C3) [NBP1-97687] - Immunoblotting result of NBP1-97687(131C3) recognizing nuclear lamins A and C in human fibroblasts.")
![Immunohistochemistry-Frozen: Lamin A + C Antibody (131C3) [NBP1-97687] - Immunohistochemistry on frozen tissue section of human skin showing nuclear lamina staining in epithelial and connective tissue cells.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-131C3-Immunohistochemistry-Frozen-NBP1-97687-img0004.jpg)
![Immunohistochemistry: Lamin A + C Antibody (131C3) [NBP1-97687] - Immunohistochemistry of on frozen sections of swine skin showing strong positive staining in nuclei of the epidermal cells and to a lesser extent in the connective tissue.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-131C3-Immunohistochemistry-NBP1-97687-img0001.jpg)
![Immunohistochemistry-Frozen: Lamin A + C Antibody (131C3) [NBP1-97687] - Immunohistochemistry of NBP1-97687(131C3) on frozen sections of human colon showing strong positive staining in nuclei of the epithelial cells and to a lesser extent in the connective tissue.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-131C3-Immunohistochemistry-Frozen-NBP1-97687-img0002.jpg)
![Western Blot: HDAC1 Antibody [NBP2-54621] - Whole cell extracts (50 ug) from HeLa cells transfected with HDAC1 siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the antibody against HDAC1 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right (expected size: 55 kDa); the marker (in kDa) is shown on the left.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Western-Blot-NBP2-54621-img0004.jpg "Western Blot: HDAC1 Antibody [NBP2-54621] - Whole cell extracts (50 ug) from HeLa cells transfected with HDAC1 siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the antibody against HDAC1 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right (expected size: 55 kDa); the marker (in kDa) is shown on the left.")
![Immunocytochemistry/Immunofluorescence: HDAC1 Antibody [NBP2-54621] - HeLa cells were stained with the antibody against HDAC1 and with DAPI. Cells were fixed with 4% formaldehyde for 10' and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the HDAC1 antibody (left) diluted 1:500 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Immunofluorescence-NBP2-54621-img0006.jpg)
![Chromatin Immunoprecipitation: HDAC1 Antibody [NBP2-54621] - ChIP was performed with the antibody against HDAC1 on sheared chromatin from 4,000,000 HeLa cells. An antibody titration consisting of 1, 2, 5 and 10 ug per ChIP experiment was analysed. IgG (2 ug/IP) was used as negative IP control. QPCR was performed with primers specific for the EIF4A2 and GAPDH promoters, used as positive controls, and for the MYOD1 gene and Sat2 satellite repeat, used as negative controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/HDAC1-Antibody-Chromatin-Immunoprecipitation-ChIP-NBP2-54621-img0001.jpg)
![Western Blot: HDAC1 Antibody [NBP2-54621] - Whole cell extracts (25 ug, lane 1) and nuclear extracts (25 ug, lane 2) from HeLa cells were analysed by Western blot using the antibody against HDAC1 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right (expected size: 55 kDa); the marker (in kDa) is shown on the left.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Western-Blot-NBP2-54621-img0003.jpg)
![ELISA: HDAC1 Antibody [NBP2-54621] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against HDAC1, crude serum and flow through. The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:75,000.](http://images.novusbio.com/fullsize/HDAC1-Antibody-ELISA-NBP2-54621-img0002.jpg)
![Protein Array: HDAC1 Antibody [NBP2-54621] - The specificity of the antibody against HDAC1 was demonstrated using a human protein microarray containing more than 19,000 human proteins. The antibody was used at a dilution of 1:100,000. The figure shows the Z-score of the signal intensity (mean value of the duplicate spots on the array). The names of the proteins with 5 highest Z-scores are indicated at the bottom. This figure clearly shows the high specificity of the antibody for HDAC1.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Protein-Array-NBP2-54621-img0005.jpg)
![Western Blot: Lamin A + C Antibody [NBP2-25152] - Western blot analysis of cytosolic or nuclear enriched fractions of cell lines probed with chicken pAb to lamin A/C, NBP2-25152, dilution 1:1,000 in green: [1] protein standard (red), [2] HeLa cytosol, [3] HeLa nuclear, [4] NIH-3T3 cytosol, and [5] NIH-3T3 nuclear fractions. Two strong bands at 74 and 65kDa correspond to lamin A and lamin C proteins respectively, detected exclusively in the nuclear fractions. The same blot was simultaneously probed with mouse mAb to GAPDH, in red. The single band at 37kDa represents GAPDH protein which is expressed predominantly in the cytosolic fractions.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-Western-Blot-NBP2-25152-img0002.jpg "Western Blot: Lamin A + C Antibody [NBP2-25152] - Western blot analysis of cytosolic or nuclear enriched fractions of cell lines probed with chicken pAb to lamin A/C, NBP2-25152, dilution 1:1,000 in green: [1] protein standard (red), [2] HeLa cytosol, [3] HeLa nuclear, [4] NIH-3T3 cytosol, and [5] NIH-3T3 nuclear fractions. Two strong bands at 74 and 65kDa correspond to lamin A and lamin C proteins respectively, detected exclusively in the nuclear fractions. The same blot was simultaneously probed with mouse mAb to GAPDH, in red. The single band at 37kDa represents GAPDH protein which is expressed predominantly in the cytosolic fractions.")
![Immunocytochemistry/Immunofluorescence: Lamin A + C Antibody [NBP2-25152] - Analysis of HeLa cells stained with chicken pAb to lamin A/C, NBP2-25152, dilution 1:2,000 in red, and costained with mouse mAb to actin, dilution 1:500, in green. The blue is Hoechst staining of nuclear DNA. The CPCA-LaminAC antibody specifically labels the nuclear lamina, while the actin antibody stains the submembranous actin-rich cytoskeleton, stress fibers and bundles of actin associated with cell adhesion sites.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-Immunofluorescence-NBP2-25152-img0001.jpg)