| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-47316-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-91985-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-94707-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-04987-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-05904-50ul | |||
![Western Blot: HERC5 Antibody [NBP1-58102] - hek293 cell lysate at 1:1000.](http://images.novusbio.com/fullsize/HERC5-Antibody-Western-Blot-NBP1-58102-img0002.jpg "Western Blot: HERC5 Antibody [NBP1-58102] - hek293 cell lysate at 1:1000.")
![Immunohistochemistry-Paraffin: HERC5 Antibody [NBP1-58102] - Formalin Fixed Paraffin; Embedded Tissue: Human Pineal Tissue; Observed Staining: Cytoplasmic in cell bodies and processes of pinealocytes; Primary Antibody Concentration: 1:100](http://images.novusbio.com/fullsize/HERC5-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-58102-img0003.jpg)
![Western Blot: HERC5 Antibody [NBP1-58102] - Titration: 0.2-1 ug/ml ELISA Titer: 1:312500 Positive Control: HepG2 cell lysate](http://images.novusbio.com/fullsize/HERC5-Antibody-Western-Blot-NBP1-58102-img0001.jpg)
![Western Blot: RFC1 Antibody [NB100-230] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RFC1 antibody (lot was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/RFC1-Antibody-Western-Blot-NB100-230-img0089.jpg "Western Blot: RFC1 Antibody [NB100-230] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RFC1 antibody (lot was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![<b>Biological Strategies Validation. </b>Western Blot: RFC1 Antibody [NB100-230] - Samples: Whole cell lysate (50, 15 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RFC1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/RFC1-Antibody-Western-Blot-NB100-230-img0088.jpg)
![Western Blot: Topoisomerase I Antibody [NBP1-30481] - Whole cell lysate from HeLa and 293T cells. TOP1 was less efficiently immunoprecipitated by rabbit anti-TOP1 antibody NBP1-30482.](http://images.novusbio.com/fullsize/Topoisomerase-I-Antibody-Western-Blot-NBP1-30481-img0002.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: Topoisomerase I Antibody [NBP1-30481] - Whole cell lysate from HeLa and 293T cells. TOP1 was less efficiently immunoprecipitated by rabbit anti-TOP1 antibody NBP1-30482.")
![Immunocytochemistry/Immunofluorescence: Rad50 Antibody [NBP2-47316] - Staining of human cell line A-431 shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Rad50-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-47316-img0003.jpg "Immunocytochemistry/Immunofluorescence: Rad50 Antibody [NBP2-47316] - Staining of human cell line A-431 shows localization to nucleoplasm. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: Rad50 Antibody [NBP2-47316] - Staining of human rectum shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/Rad50-Antibody-Immunohistochemistry-NBP2-47316-img0002.jpg)
![Western Blot: Rad50 Antibody (5M2K10) [NBP3-16286] - Western blot analysis of extracts of various cell lines, using Rad50 Rabbit mAb (NBP3-16286) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/Rad50-Antibody-5M2K10-Western-Blot-NBP3-16286-img0001.jpg "Western Blot: Rad50 Antibody (5M2K10) [NBP3-16286] - Western blot analysis of extracts of various cell lines, using Rad50 Rabbit mAb (NBP3-16286) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Immunoprecipitation: Rad50 Antibody (5M2K10) [NBP3-16286] - Immunoprecipitation analysis of 300ug extracts of K-562 cells using 3ug Rad50 antibody (NBP3-16286). Western blot was performed from the immunoprecipitate using Rad50 antibody (NBP3-16286) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/Rad50-Antibody-5M2K10-Immunoprecipitation-NBP3-16286-img0002.jpg)
![Immunohistochemistry-Paraffin: HERC5 Antibody [NBP1-91985] Staining of human testis shows strong cytoplasmic positivity in the spermatogonia.](http://images.novusbio.com/fullsize/HERC5-Antibody-Immunohistochemistry-Paraffin-NBP1-91985-img0002.jpg "Immunohistochemistry-Paraffin: HERC5 Antibody [NBP1-91985] Staining of human testis shows strong cytoplasmic positivity in the spermatogonia.")
![Western Blot: MPHOSPH1 Antibody [NBP2-94707] - Analysis of extracts of various cell lines, using MPHOSPH1 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.](http://images.novusbio.com/fullsize/MPHOSPH1-Antibody-Western-Blot-NBP2-94707-img0001.jpg "Western Blot: MPHOSPH1 Antibody [NBP2-94707] - Analysis of extracts of various cell lines, using MPHOSPH1 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.")
![Western Blot: HERC5 Antibody [NBP3-04987] - Analysis of extracts of various cell lines, using HERC5 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit](http://images.novusbio.com/fullsize/HERC5-Antibody-Western-Blot-NBP3-04987-img0001.jpg "Western Blot: HERC5 Antibody [NBP3-04987] - Analysis of extracts of various cell lines, using HERC5 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit")
![Immunohistochemistry-Paraffin: MPHOSPH1 Antibody [NBP3-05904] - Staining of human KIF20B in human small intestine with rabbit polyclonal antibody at 1:100.](http://images.novusbio.com/fullsize/MPHOSPH1-Antibody-Immunohistochemistry-Paraffin-NBP3-05904-img0002.jpg "Immunohistochemistry-Paraffin: MPHOSPH1 Antibody [NBP3-05904] - Staining of human KIF20B in human small intestine with rabbit polyclonal antibody at 1:100.")
![Immunohistochemistry-Paraffin: MPHOSPH1 Antibody [NBP3-05904] - Staining of human KIF20B in human kidney with rabbit polyclonal antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/MPHOSPH1-Antibody-Immunohistochemistry-Paraffin-NBP3-05904-img0001.jpg)
![Western Blot: Rad50 Antibody (JE60-04) [NBP3-32890] - Western blot analysis of Rad50 on different lysates with Rabbit anti-Rad50 antibody (NBP3-32890) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: K-562 cell lysate (20 ug/Lane) Lane 3: U-87 MG cell lysate (20 ug/Lane) Lane 4: U-937 cell lysate (20 ug/Lane) Lane 5: Caco-2 cell lysate (20 ug/Lane) Lane 6: Mouse testis tissue lysate (40 ug/Lane) Lane 7: Rat testis tissue lysate (40 ug/Lane) Predicted band size: 154 kDa Observed band size: 154 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32890) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32890_rabbit-rad50-mab-je60-04-288202412461633.jpg "Western Blot: Rad50 Antibody (JE60-04) [NBP3-32890] - Western blot analysis of Rad50 on different lysates with Rabbit anti-Rad50 antibody (NBP3-32890) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: K-562 cell lysate (20 ug/Lane) Lane 3: U-87 MG cell lysate (20 ug/Lane) Lane 4: U-937 cell lysate (20 ug/Lane) Lane 5: Caco-2 cell lysate (20 ug/Lane) Lane 6: Mouse testis tissue lysate (40 ug/Lane) Lane 7: Rat testis tissue lysate (40 ug/Lane) Predicted band size: 154 kDa Observed band size: 154 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32890) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")