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![Western Blot: CCAR1 Antibody [NBP3-35368] - Western blot analysis of various lysates using CCAR1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.](http://images.novusbio.com/fullsize/nbp3-35368_rabbit-ccar1-pab-912202415133430.jpg "Western Blot: CCAR1 Antibody [NBP3-35368] - Western blot analysis of various lysates using CCAR1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.")
![Immunoprecipitation: CCAR1 Antibody [NBP3-35368] - Immunoprecipitation analysis of 200 ug extracts of HeLa cells using 3 ug CCAR1 antibody. Western blot was performed from the immunoprecipitate using CCAR1 antibody at a dilution of 1:500.](http://images.novusbio.com/fullsize/nbp3-35368_rabbit-ccar1-pab-9122024141819.jpg)
![Western Blot: CCAR1 Antibody [NB500-188] - Detection of Human CCAR1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CCAR1 antibody NB500-188 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/CCAR1-Antibody-Western-Blot-NB500-188-img0003.jpg "<b>Biological Strategies Validation. </b>Western Blot: CCAR1 Antibody [NB500-188] - Detection of Human CCAR1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CCAR1 antibody NB500-188 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: CCAR1 Antibody [NB500-188] - on HeLa whole cell lysate using NB500-188. CCAR1 was also immunoprecipitated with another rabbit anti-CCAR1 antibody, NB500-187, and NB500-188. Immunoprecipitated CCAR1 was blotted using NB500-188.](http://images.novusbio.com/fullsize/CCAR1-Antibody-Western-Blot-NB500-188-img0001.jpg)
![Western Blot: BTG4 Antibody [H00054766-B01P] - Analysis of BTG4 expression in transfected 293T cell line by BTG4 polyclonal antibody. Lane 1: BTG4 transfected lysate(22.66 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/BTG4-Antibody-Western-Blot-H00054766-B01P-img0004.jpg "Western Blot: BTG4 Antibody [H00054766-B01P] - Analysis of BTG4 expression in transfected 293T cell line by BTG4 polyclonal antibody. Lane 1: BTG4 transfected lysate(22.66 KDa). Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: BTG4 Antibody [H00054766-B01P] - Analysis of purified antibody to BTG4 on HeLa cell. (antibody concentration 10 ug/ml)](http://images.novusbio.com/fullsize/BTG4-Antibody-Immunocytochemistry-Immunofluorescence-H00054766-B01P-img0003.jpg)
![Western Blot: BTG4 Antibody (1A6) [H00054766-M07] - Analysis of BTG4 expression in transfected 293T cell line by BTG4 monoclonal antibody (M07), clone 1A6. Lane 1: BTG4 transfected lysatE (24 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/BTG4-Antibody-1A6-Western-Blot-H00054766-M07-img0004.jpg "Western Blot: BTG4 Antibody (1A6) [H00054766-M07] - Analysis of BTG4 expression in transfected 293T cell line by BTG4 monoclonal antibody (M07), clone 1A6. Lane 1: BTG4 transfected lysatE (24 KDa). Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: BTG4 Antibody (1A6) [H00054766-M07] - Analysis of monoclonal antibody to BTG4 on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/BTG4-Antibody-1A6-Immunocytochemistry-Immunofluorescence-H00054766-M07-img0003.jpg)
![ELISA: Rat COMP/Thrombospondin-5 ELISA Kit (Colorimetric) [NBP2-82142] - Samples were spiked with high concentrations of Rat COMP/Thrombospondin-5 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-82142_rat-comp-thrombospondin-5-elisa-kit-colorimetric-13220241626153.jpg "ELISA: Rat COMP/Thrombospondin-5 ELISA Kit (Colorimetric) [NBP2-82142] - Samples were spiked with high concentrations of Rat COMP/Thrombospondin-5 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Rat COMP/Thrombospondin-5 ELISA Kit (Colorimetric) [NBP2-82142] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-82142_rat-comp-thrombospondin-5-elisa-kit-colorimetric-432024924078.jpg)
![Western Blot: CCAR1 Antibody [NBP1-46158] - NBP1-46158 (1ug/ml) staining of Rat Skeletal Muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/CCAR1-Antibody-Western-Blot-NBP1-46158-img0002.jpg "Western Blot: CCAR1 Antibody [NBP1-46158] - NBP1-46158 (1ug/ml) staining of Rat Skeletal Muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: COMP/Thrombospondin-5 Antibody (C1) [NBP3-41565] - DAB staining on IHC-P; Samples: Mouse Testis Tissue; Primary Ab: 30ug/ml NBP3-41565; Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-41565_mouse-comp-thrombospondin-5-mab-c1-6122024205889.jpg "Immunohistochemistry-Paraffin: COMP/Thrombospondin-5 Antibody (C1) [NBP3-41565] - DAB staining on IHC-P; Samples: Mouse Testis Tissue; Primary Ab: 30ug/ml NBP3-41565; Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody")
![Western Blot: MAD4 Antibody [NB100-1117] - (0.5 ug/ml) of human kidney lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence](http://images.novusbio.com/fullsize/MAD4-Antibody-Western-Blot-NB100-1117-img0001.jpg "Western Blot: MAD4 Antibody [NB100-1117] - (0.5 ug/ml) of human kidney lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence")
![Western Blot: TSH R Antibody [NBP1-53167] - Jurkat Lane A: Primary Antibody Lane B: Primary Antibody + Blocking Peptide Primary Antibody Concentration: 2.5 ug/ml Peptide Concentration: 2.0 ug/ml lysate Quantity: 25ug/ Lane Gel Concentration: 12% There is BioGPS gene expression data showing that TSHR is expressed in Jurkat.](http://images.novusbio.com/fullsize/TSH-R-Antibody-Western-Blot-NBP1-53167-img0005.jpg "Western Blot: TSH R Antibody [NBP1-53167] - Jurkat Lane A: Primary Antibody Lane B: Primary Antibody + Blocking Peptide Primary Antibody Concentration: 2.5 ug/ml Peptide Concentration: 2.0 ug/ml lysate Quantity: 25ug/ Lane Gel Concentration: 12% There is BioGPS gene expression data showing that TSHR is expressed in Jurkat.")
![Western Blot: TSH R Antibody [NBP1-53167] - Jurkat cell lysate, concentration 1.25ug/ml.](http://images.novusbio.com/fullsize/TSH-R-Antibody-Western-Blot-NBP1-53167-img0002.jpg)
![Immunohistochemistry-Paraffin: MAD4 Antibody [NBP3-10957] - Immunohistochemical analysis of formalin-fixed paraffin-embedded pneumocyte nuclei in human lung tissue using NBP3-10957 at a dilution of 1:100. Donkey anti-Rabbit-Cy3 used as a secondary antibody at a dilution of 1:200. 20X magnification.](http://images.novusbio.com/fullsize/MAD4-Antibody-Immunohistochemistry-Paraffin-NBP3-10957-img0001.jpg "Immunohistochemistry-Paraffin: MAD4 Antibody [NBP3-10957] - Immunohistochemical analysis of formalin-fixed paraffin-embedded pneumocyte nuclei in human lung tissue using NBP3-10957 at a dilution of 1:100. Donkey anti-Rabbit-Cy3 used as a secondary antibody at a dilution of 1:200. 20X magnification.")
![Immunohistochemistry-Paraffin: BTG4 Antibody [NBP3-05926] - Staining of human BTG4 in human ovarian cancer with rabbit polyclonal antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/BTG4-Antibody-Immunohistochemistry-Paraffin-NBP3-05926-img0002.jpg "Immunohistochemistry-Paraffin: BTG4 Antibody [NBP3-05926] - Staining of human BTG4 in human ovarian cancer with rabbit polyclonal antibody at 1:100 dilution.")
![Immunohistochemistry-Paraffin: BTG4 Antibody [NBP3-05926] - Staining of human BTG4 in human testis with rabbit polyclonal antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/BTG4-Antibody-Immunohistochemistry-Paraffin-NBP3-05926-img0001.jpg)