| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44477 | |||
| BSA Free | NBP2-36762-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-18565-25ul | |||
| Azide and BSA Free | NBP3-44373 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52464-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37490-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-57349-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-57569-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-49172-25ul | |||
![Western Blot: FRA-1/FOSL1 Antibody (OTI12F9) - Azide and BSA Free [NBP2-71351] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FOSL1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FOSL1.](http://images.novusbio.com/fullsize/FRA-1-FOSL1-Antibody-OTI12F9-Azide-and-BSA-Free-Western-Blot-NBP2-71351-img0001.jpg "Western Blot: FRA-1/FOSL1 Antibody (OTI12F9) - Azide and BSA Free [NBP2-71351] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FOSL1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FOSL1.")
![Immunohistochemistry: FRA-1/FOSL1 Antibody (OTI12F9) - Azide and BSA Free [NBP2-71351] - Staining of paraffin-embedded Ovary tissue using anti-FOSL1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/FRA-1-FOSL1-Antibody-OTI12F9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71351-img0009.jpg)
![Immunohistochemistry: FRA-1/FOSL1 Antibody (OTI12F9) - Azide and BSA Free [NBP2-71351] - Carcinoma of bladder tissue.](http://images.novusbio.com/fullsize/FRA-1-FOSL1-Antibody-OTI12F9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71351-img0002.jpg)
![Immunohistochemistry: FRA-1/FOSL1 Antibody (OTI12F9) - Azide and BSA Free [NBP2-71351] - Staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-FOSL1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/FRA-1-FOSL1-Antibody-OTI12F9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71351-img0003.jpg)
![Immunohistochemistry: FRA-1/FOSL1 Antibody (OTI12F9) - Azide and BSA Free [NBP2-71351] - Staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-FOSL1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/FRA-1-FOSL1-Antibody-OTI12F9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71351-img0004.jpg)
![Immunohistochemistry: FRA-1/FOSL1 Antibody (OTI12F9) - Azide and BSA Free [NBP2-71351] - Staining of paraffin-embedded colon tissue using anti-FOSL1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/FRA-1-FOSL1-Antibody-OTI12F9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71351-img0005.jpg)
![Immunohistochemistry: FRA-1/FOSL1 Antibody (OTI12F9) - Azide and BSA Free [NBP2-71351] - Staining of paraffin-embedded endometrium tissue using anti-FOSL1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/FRA-1-FOSL1-Antibody-OTI12F9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71351-img0006.jpg)
![Immunohistochemistry: FRA-1/FOSL1 Antibody (OTI12F9) - Azide and BSA Free [NBP2-71351] - Staining of paraffin-embedded Kidney tissue using anti-FOSL1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/FRA-1-FOSL1-Antibody-OTI12F9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71351-img0007.jpg)
![Immunohistochemistry: FRA-1/FOSL1 Antibody (OTI12F9) - Azide and BSA Free [NBP2-71351] - Staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-FOSL1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/FRA-1-FOSL1-Antibody-OTI12F9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71351-img0008.jpg)
![Immunohistochemistry: FRA-1/FOSL1 Antibody (OTI12F9) - Azide and BSA Free [NBP2-71351] - Staining of paraffin-embedded prostate tissue using anti-FOSL1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/FRA-1-FOSL1-Antibody-OTI12F9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71351-img0010.jpg)
![Western Blot: Ezrin Antibody (CL2378) [NBP2-36762] - Lane 1: Marker [kDa]. Lane 2: Human cell line U-251 MG](http://images.novusbio.com/fullsize/Ezrin-Antibody-CL2378-Western-Blot-NBP2-36762-img0005.jpg "Western Blot: Ezrin Antibody (CL2378) [NBP2-36762] - Lane 1: Marker [kDa]. Lane 2: Human cell line U-251 MG")
![Immunocytochemistry/Immunofluorescence: Ezrin Antibody (CL2378) [NBP2-36762] - Staining of A-431 cells using the Anti-EZR monoclonal antibody, showing specific staining in the plasma membrane in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).](http://images.novusbio.com/fullsize/Ezrin-Antibody-CL2378-Immunocytochemistry-Immunofluorescence-NBP2-36762-img0006.jpg)
![Immunohistochemistry: Ezrin Antibody (CL2378) [NBP2-36762] - Staining of human skeletal muscle shows absence of immunoreactivity.](http://images.novusbio.com/fullsize/Ezrin-Antibody-CL2378-Immunohistochemistry-NBP2-36762-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Ezrin Antibody (CL2378) [NBP2-36762] - Staining of U-251 cells using the Anti-EZR monoclonal antibody, showing specific staining in the plasma membrane in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).](http://images.novusbio.com/fullsize/Ezrin-Antibody-CL2378-Immunocytochemistry-Immunofluorescence-NBP2-36762-img0007.jpg)
![Immunohistochemistry: Ezrin Antibody (CL2378) [NBP2-36762] - Staining of human cervix shows strong membranous immunoreactivity in the epithelial cells.](http://images.novusbio.com/fullsize/Ezrin-Antibody-CL2378-Immunohistochemistry-NBP2-36762-img0001.jpg)
![Immunohistochemistry: Ezrin Antibody (CL2378) [NBP2-36762] - Staining of human placenta shows strong membranous and moderate cytoplasmic positivity in the trophoblast.](http://images.novusbio.com/fullsize/Ezrin-Antibody-CL2378-Immunohistochemistry-NBP2-36762-img0002.jpg)
![Immunohistochemistry: Ezrin Antibody (CL2378) [NBP2-36762] - Staining of human prostate cancer shows strong membranous and moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Ezrin-Antibody-CL2378-Immunohistochemistry-NBP2-36762-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: PELO Antibody [NBP2-19769] - Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PELO protein stained by PELO antibody diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/PELO-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-19769-img0004.jpg "Immunocytochemistry/Immunofluorescence: PELO Antibody [NBP2-19769] - Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PELO protein stained by PELO antibody diluted at 1:500. Blue: Hoechst 33342 staining.")
![Immunohistochemistry-Paraffin: PELO Antibody [NBP2-19769] - Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/PELO-Antibody-Immunohistochemistry-Paraffin-NBP2-19769-img0005.jpg)
![Western Blot: PELO Antibody [NBP2-19769] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PELO antibody (NBP2-19769) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-19769_rabbit-polyclonal-pelo-antibody-910202420122036.jpg)
![Western Blot: GSPT1 Antibody (CL13332) [NBP3-18565] - Analysis in human cell line K562.](http://images.novusbio.com/fullsize/GSPT1-Antibody-CL13332-Western-Blot-NBP3-18565-img0007.jpg "Western Blot: GSPT1 Antibody (CL13332) [NBP3-18565] - Analysis in human cell line K562.")
![Immunohistochemistry-Paraffin: GSPT1 Antibody (CL13332) [NBP3-18565] - Staining of human liver shows no positivity in hepatocytes.](http://images.novusbio.com/fullsize/GSPT1-Antibody-CL13332-Immunohistochemistry-Paraffin-NBP3-18565-img0013.jpg)
![Immunohistochemistry-Paraffin: GSPT1 Antibody (CL13332) [NBP3-18565] - Staining of human breast cancer shows strong cytoplasmic positivity in tumor cells.](http://images.novusbio.com/fullsize/GSPT1-Antibody-CL13332-Immunohistochemistry-Paraffin-NBP3-18565-img0008.jpg)
![Immunohistochemistry-Paraffin: GSPT1 Antibody (CL13332) [NBP3-18565] - Staining of human ovarian cancer (papillary carcinoma) shows strong cytoplasmic positivity in tumor cells.](http://images.novusbio.com/fullsize/GSPT1-Antibody-CL13332-Immunohistochemistry-Paraffin-NBP3-18565-img0009.jpg)
![Immunohistochemistry-Paraffin: GSPT1 Antibody (CL13332) [NBP3-18565] - Staining of human colorectal cancer (metastasis to ovary) shows strong cytoplasmic positivity in tumor cells.](http://images.novusbio.com/fullsize/GSPT1-Antibody-CL13332-Immunohistochemistry-Paraffin-NBP3-18565-img0010.jpg)
![Immunohistochemistry-Paraffin: GSPT1 Antibody (CL13332) [NBP3-18565] - Staining of human colorectal cancer shows strong cytoplasmic positivity in tumor cells.](http://images.novusbio.com/fullsize/GSPT1-Antibody-CL13332-Immunohistochemistry-Paraffin-NBP3-18565-img0011.jpg)
![Immunohistochemistry-Paraffin: GSPT1 Antibody (CL13332) [NBP3-18565] - Staining of human testis shows moderate to strong cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/GSPT1-Antibody-CL13332-Immunohistochemistry-Paraffin-NBP3-18565-img0012.jpg)
![Western Blot: Ezrin Antibody (6F1C3) [NBP2-52464] - Analysis using EZR mouse mAb against Jurkat (1), A431 (2), MCF-7 (3) and Hela (4) cell lysate.](http://images.novusbio.com/fullsize/Ezrin-Antibody-6F1C3-Western-Blot-NBP2-52464-img0005.jpg "Western Blot: Ezrin Antibody (6F1C3) [NBP2-52464] - Analysis using EZR mouse mAb against Jurkat (1), A431 (2), MCF-7 (3) and Hela (4) cell lysate.")
![Immunocytochemistry/Immunofluorescence: Ezrin Antibody (6F1C3) [NBP2-52464] - Immunofluorescence analysis of Hela cells using EZR mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize/Ezrin-Antibody-6F1C3-Immunofluorescence-NBP2-52464-img0002.jpg)
![Flow Cytometry: Ezrin Antibody (6F1C3) [NBP2-52464] - Analysis of A431 cells using EZR mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Ezrin-Antibody-6F1C3-Flow-Cytometry-NBP2-52464-img0001.jpg)
![Western Blot: Ezrin Antibody (6F1C3) [NBP2-52464] - Analysis using EZR mAb against HEK293 (1) and EZR (AA: 292-464)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Ezrin-Antibody-6F1C3-Western-Blot-NBP2-52464-img0004.jpg)
![Western Blot: Ezrin Antibody (6F1C3) [NBP2-52464] - Analysis using EZR mAb against human EZR (AA: 292-464) recombinant protein. (Expected MW is 47.1 kDa)](http://images.novusbio.com/fullsize/Ezrin-Antibody-6F1C3-Western-Blot-NBP2-52464-img0003.jpg)
![ELISA: Ezrin Antibody (6F1C3) [NBP2-52464] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).](http://images.novusbio.com/fullsize/Ezrin-Antibody-6F1C3-ELISA-NBP2-52464-img0006.jpg)
![Western Blot: NCK1 Antibody (5B7) [NBP2-37490] - Western blot analysis using NCK1 mouse mAb against Jurkat (1), HeLa (2), HEK293 (3), A431 (4), K562 (5), and COS7 (6) cell lysate.](http://images.novusbio.com/fullsize/NCK1-Antibody-5B7-Western-Blot-NBP2-37490-img0003.jpg "Western Blot: NCK1 Antibody (5B7) [NBP2-37490] - Western blot analysis using NCK1 mouse mAb against Jurkat (1), HeLa (2), HEK293 (3), A431 (4), K562 (5), and COS7 (6) cell lysate.")
![Immunohistochemistry: NCK1 Antibody (5B7) [NBP2-37490] - Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using NCK1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/NCK1-Antibody-5B7-Immunohistochemistry-NBP2-37490-img0005.jpg)
![Flow Cytometry: NCK1 Antibody (5B7) [NBP2-37490] - Flow cytometric analysis of Jurkat cells using NCK1 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/NCK1-Antibody-5B7-Flow-Cytometry-NBP2-37490-img0004.jpg)
![Western Blot: NCK1 Antibody (5B7) [NBP2-37490] - Western blot analysis using NCK1 mAb against human NCK1 (AA: 203-371) recombinant protein. (Expected MW is 44.9 kDa)](http://images.novusbio.com/fullsize/NCK1-Antibody-5B7-Western-Blot-NBP2-37490-img0002.jpg)
![ELISA: NCK1 Antibody (5B7) [NBP2-37490] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/NCK1-Antibody-5B7-ELISA-NBP2-37490-img0001.jpg)
![Immunohistochemistry-Paraffin: Cyclin F Antibody [NBP2-57349] - Staining of human liver.](http://images.novusbio.com/fullsize/Cyclin-F-Antibody-Immunohistochemistry-Paraffin-NBP2-57349-img0007.jpg "Immunohistochemistry-Paraffin: Cyclin F Antibody [NBP2-57349] - Staining of human liver.")
![Immunohistochemistry-Paraffin: Cyclin F Antibody [NBP2-57349] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/Cyclin-F-Antibody-Immunohistochemistry-Paraffin-NBP2-57349-img0002.jpg)
![Immunohistochemistry-Paraffin: Cyclin F Antibody [NBP2-57349] - Staining of human tonsil shows high expression.](http://images.novusbio.com/fullsize/Cyclin-F-Antibody-Immunohistochemistry-Paraffin-NBP2-57349-img0003.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Cyclin F Antibody [NBP2-57349] - Staining in human tonsil and pancreas tissues using anti-CCNF antibody. Corresponding CCNF RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Cyclin-F-Antibody-Immunohistochemistry-Paraffin-NBP2-57349-img0004.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Cyclin F Antibody [NBP2-57349] - Staining of human liver, lymph node, pancreas and skin using Anti-CCNF antibody NBP2-57349 (A) shows similar protein distribution across tissues to independent antibody NBP2-57569 (B).](http://images.novusbio.com/fullsize/Cyclin-F-Antibody-Immunohistochemistry-Paraffin-NBP2-57349-img0005.jpg)
![Immunohistochemistry-Paraffin: Cyclin F Antibody [NBP2-57349] - Staining of human lymph node.](http://images.novusbio.com/fullsize/Cyclin-F-Antibody-Immunohistochemistry-Paraffin-NBP2-57349-img0006.jpg)
![Immunohistochemistry-Paraffin: Cyclin F Antibody [NBP2-57349] - Staining of human skin.](http://images.novusbio.com/fullsize/Cyclin-F-Antibody-Immunohistochemistry-Paraffin-NBP2-57349-img0008.jpg)
![Immunohistochemistry-Paraffin: Cyclin F Antibody [NBP2-57569] - Staining of human liver.](http://images.novusbio.com/fullsize/Cyclin-F-Antibody-Immunohistochemistry-Paraffin-NBP2-57569-img0008.jpg "Immunohistochemistry-Paraffin: Cyclin F Antibody [NBP2-57569] - Staining of human liver.")
![Immunohistochemistry-Paraffin: Cyclin F Antibody [NBP2-57569] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/Cyclin-F-Antibody-Immunohistochemistry-Paraffin-NBP2-57569-img0002.jpg)
![Immunohistochemistry-Paraffin: Cyclin F Antibody [NBP2-57569] - Staining of human tonsil shows high expression.](http://images.novusbio.com/fullsize/Cyclin-F-Antibody-Immunohistochemistry-Paraffin-NBP2-57569-img0003.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Cyclin F Antibody [NBP2-57569] - Staining in human tonsil and pancreas tissues using anti-CCNF antibody. Corresponding CCNF RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Cyclin-F-Antibody-Immunohistochemistry-Paraffin-NBP2-57569-img0004.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Cyclin F Antibody [NBP2-57569] - Staining of human liver, lymph node, pancreas and skin using Anti-CCNF antibody NBP2-57569 (A) shows similar protein distribution across tissues to independent antibody NBP2-57349 (B).](http://images.novusbio.com/fullsize/Cyclin-F-Antibody-Immunohistochemistry-Paraffin-NBP2-57569-img0005.jpg)
![Immunohistochemistry-Paraffin: Cyclin F Antibody [NBP2-57569] - Staining of human skin.](http://images.novusbio.com/fullsize/Cyclin-F-Antibody-Immunohistochemistry-Paraffin-NBP2-57569-img0006.jpg)
![Immunohistochemistry-Paraffin: Cyclin F Antibody [NBP2-57569] - Staining of human lymph node.](http://images.novusbio.com/fullsize/Cyclin-F-Antibody-Immunohistochemistry-Paraffin-NBP2-57569-img0007.jpg)
![Western Blot: p190RhoGAP Antibody [NBP2-49172] - Analysis in human cell line WM-115.](http://images.novusbio.com/fullsize/p190RhoGAP-Antibody-Western-Blot-NBP2-49172-img0006.jpg "Western Blot: p190RhoGAP Antibody [NBP2-49172] - Analysis in human cell line WM-115.")
![Immunohistochemistry-Paraffin: p190RhoGAP Antibody [NBP2-49172] - Staining of human fallopian tube shows strong membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/p190RhoGAP-Antibody-Immunohistochemistry-Paraffin-NBP2-49172-img0005.jpg)
![Immunohistochemistry-Paraffin: p190RhoGAP Antibody [NBP2-49172] - Staining of human breast shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/p190RhoGAP-Antibody-Immunohistochemistry-NBP2-49172-img0001.jpg)
![Immunohistochemistry-Paraffin: p190RhoGAP Antibody [NBP2-49172] - Staining of human testis shows moderate membranous and cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/p190RhoGAP-Antibody-Immunohistochemistry-Paraffin-NBP2-49172-img0002.jpg)
![Immunohistochemistry-Paraffin: p190RhoGAP Antibody [NBP2-49172] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.](http://images.novusbio.com/fullsize/p190RhoGAP-Antibody-Immunohistochemistry-Paraffin-NBP2-49172-img0003.jpg)
![Immunohistochemistry-Paraffin: p190RhoGAP Antibody [NBP2-49172] - Staining of human heart muscle shows strong cytoplasmic positivity in cardiomyocytes.](http://images.novusbio.com/fullsize/p190RhoGAP-Antibody-Immunohistochemistry-Paraffin-NBP2-49172-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: GSPT1 Antibody (HL1345) - Azide and BSA Free [NBP3-25492] - GSPT1 antibody [HL1345] detects GSPT1 protein at cytoplasm by immunofluorescent analysis. Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GSPT1 stained by GSPT1 antibody [HL1345] (NBP3-25492) diluted at 1:500. Blue: Fluoroshield with DAPI . Scale bar= 10um.](http://images.novusbio.com/fullsize/nbp3-25492_rabbit-gspt1-mab-hl1345-azide-and-bsa-free-21202493917.jpg "Immunocytochemistry/Immunofluorescence: GSPT1 Antibody (HL1345) - Azide and BSA Free [NBP3-25492] - GSPT1 antibody [HL1345] detects GSPT1 protein at cytoplasm by immunofluorescent analysis. Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GSPT1 stained by GSPT1 antibody [HL1345] (NBP3-25492) diluted at 1:500. Blue: Fluoroshield with DAPI . Scale bar= 10um.")
![Western Blot: GSPT1 Antibody (HL1345) - Azide and BSA Free [NBP3-25492] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with GSPT1 antibody [HL1345] (NBP3-25492) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25492_rabbit-gspt1-mab-hl1345-azide-and-bsa-free-212024939192.jpg)
![Immunohistochemistry-Paraffin: GSPT1 Antibody (HL1345) - Azide and BSA Free [NBP3-25492] - GSPT1 antibody [HL1345] detects GSPT1 protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. GSPT1 stained by GSPT1 antibody [HL1345] (NBP3-25492) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25492_rabbit-gspt1-mab-hl1345-azide-and-bsa-free-212024940861.jpg)
![Western Blot: GSPT1 Antibody (HL1345) - Azide and BSA Free [NBP3-25492] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GSPT1 antibody [HL1345] (NBP3-25492) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25492_rabbit-gspt1-mab-hl1345-azide-and-bsa-free-2120249403866.jpg)
![Western Blot: NCK1 Antibody (4R1N9) [NBP3-16759] - Western blot analysis of extracts of various cell lines, using NCK11 Rabbit mAb (NBP3-16759) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/NCK1-Antibody-4R1N9-Western-Blot-NBP3-16759-img0003.jpg "Western Blot: NCK1 Antibody (4R1N9) [NBP3-16759] - Western blot analysis of extracts of various cell lines, using NCK11 Rabbit mAb (NBP3-16759) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunocytochemistry/Immunofluorescence: NCK1 Antibody (4R1N9) [NBP3-16759] - Immunofluorescence analysis of NIH-3T3 cells using NCK11 Rabbit mAb (NBP3-16759) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/NCK1-Antibody-4R1N9-Immunocytochemistry-Immunofluorescence-NBP3-16759-img0002.jpg)
![Immunohistochemistry-Paraffin: NCK1 Antibody (4R1N9) [NBP3-16759] - Immunohistochemistry of paraffin-embedded rat testis using NCK11 Rabbit mAb (NBP3-16759) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/NCK1-Antibody-4R1N9-Immunohistochemistry-Paraffin-NBP3-16759-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: NCK1 Antibody (4R1N9) [NBP3-16759] - Immunofluorescence analysis of NIH-3T3 cells using NCK11 Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-16759_rabbit-nck1-mab-4r1n9-52202511374179.jpg)
![Western Blot: FRA-1/FOSL1 Antibody (JE33-20) [NBP3-32350] - Western blot analysis of FRA-1/FOSL1 on different lysates with Rabbit anti-FRA-1/FOSL1 antibody (NBP3-32350) at 1/1,000 dilution. Lane 1: MDA-MB-231 cell lysate Lane 2: U-87 MG cell lysate Lane 3: A431 cell lysate Lane 4: HeLa cell lysate (negative) Lane 5: HEK-293 cell lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 29 kDa Observed band size: 37 kDa (40 kDa unknown) Exposure time: 35 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32350) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32350_rabbit-fra-1-fosl1-mab-je33-20-278202416331227.jpg "Western Blot: FRA-1/FOSL1 Antibody (JE33-20) [NBP3-32350] - Western blot analysis of FRA-1/FOSL1 on different lysates with Rabbit anti-FRA-1/FOSL1 antibody (NBP3-32350) at 1/1,000 dilution. Lane 1: MDA-MB-231 cell lysate Lane 2: U-87 MG cell lysate Lane 3: A431 cell lysate Lane 4: HeLa cell lysate (negative) Lane 5: HEK-293 cell lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 29 kDa Observed band size: 37 kDa (40 kDa unknown) Exposure time: 35 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32350) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: FRA-1/FOSL1 Antibody (JE33-20) [NBP3-32350] - Immunohistochemical analysis of paraffin-embedded human cervical carcinoma tissue with Rabbit anti-FRA-1/FOSL1 antibody (NBP3-32350) at 1/3,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32350) at 1/3,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32350_rabbit-fra-1-fosl1-mab-je33-20-29820241435263.jpg)
![Immunocytochemistry/ Immunofluorescence: FRA-1/FOSL1 Antibody (JE33-20) [NBP3-32350] - Immunocytochemistry analysis of U-87 MG cells labeling FRA-1/FOSL1 with Rabbit anti-FRA-1/FOSL1 antibody (NBP3-32350) at 1/100 dilution. Cells were fixed in 100% methanol for 10 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 1% BSA for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-FRA-1/FOSL1 antibody (NBP3-32350) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32350_rabbit-fra-1-fosl1-mab-je33-20-29202410484854.jpg)
![Flow Cytometry: FRA-1/FOSL1 Antibody (JE33-20) [NBP3-32350] - Flow cytometric analysis of U-87 MG cells labeling FRA-1/FOSL1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32350, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32350_rabbit-fra-1-fosl1-mab-je33-20-29202416453260.jpg)