| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-93798-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-30536-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-49354-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-92963-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | MAB33541-SP | |||
![Western Blot: ARHGEF1 Antibody (4C4) [H00009138-M03] - Analysis of ARHGEF1 expression in K-562 (Cat # L009V1).](http://images.novusbio.com/fullsize/ARHGEF1-Antibody-4C4-Western-Blot-H00009138-M03-img0005.jpg "Western Blot: ARHGEF1 Antibody (4C4) [H00009138-M03] - Analysis of ARHGEF1 expression in K-562 (Cat # L009V1).")
![Immunocytochemistry/Immunofluorescence: ARHGEF1 Antibody (4C4) [H00009138-M03] - Analysis of monoclonal antibody to ARHGEF1 on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/ARHGEF1-Antibody-4C4-Immunocytochemistry-Immunofluorescence-H00009138-M03-img0004.jpg)
![ELISA: ARHGEF1 Antibody (4C4) [H00009138-M03] - Detection limit for recombinant GST tagged ARHGEF1 is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/ARHGEF1-Antibody-4C4-ELISA-H00009138-M03-img0003.jpg)
![Western Blot: B7-H4 Antibody [NBP1-06096] - Staining of HEK293 iso 1 + iso 2 cell lysate (A) + peptide (B). Human Adrenal Gland iso 1 (C), Human Adrenal Gland iso 2 (D) with antibody at 1 ug/mL and Human pancreas iso 1 (E) lysate with antibody at 0.1 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/B7-H4-Antibody-Western-Blot-NBP1-06096-img0001.jpg "Western Blot: B7-H4 Antibody [NBP1-06096] - Staining of HEK293 iso 1 + iso 2 cell lysate (A) + peptide (B). Human Adrenal Gland iso 1 (C), Human Adrenal Gland iso 2 (D) with antibody at 1 ug/mL and Human pancreas iso 1 (E) lysate with antibody at 0.1 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: B7-H4 Antibody [NBP1-06096] - Analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing membrane/cell junction and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/B7-H4-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-06096-img0002.jpg)
![Flow Cytometry: B7-H4 Antibody [NBP1-06096] - Analysis of paraformaldehyde fixed MCF7 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/B7-H4-Antibody-Flow-Cytometry-NBP1-06096-img0003.jpg)
![Western Blot: PPP2R2A Antibody [NBP2-93798] - Analysis of extracts of various cell lines, using PPP2R2A at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.](http://images.novusbio.com/fullsize/PPP2R2A-Antibody-Western-Blot-NBP2-93798-img0004.jpg "Western Blot: PPP2R2A Antibody [NBP2-93798] - Analysis of extracts of various cell lines, using PPP2R2A at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.")
![Immunohistochemistry-Paraffin: PPP2R2A Antibody [NBP2-93798] - Rat kidney using PPP2R2A antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/PPP2R2A-Antibody-Immunohistochemistry-Paraffin-NBP2-93798-img0003.jpg)
![Immunohistochemistry-Paraffin: PPP2R2A Antibody [NBP2-93798] - Human esophagus using PPP2R2A antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/PPP2R2A-Antibody-Immunohistochemistry-Paraffin-NBP2-93798-img0001.jpg)
![Immunohistochemistry-Paraffin: PPP2R2A Antibody [NBP2-93798] - Mouse liver using /PPP2R2A antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/PPP2R2A-Antibody-Immunohistochemistry-Paraffin-NBP2-93798-img0002.jpg)
![Western Blot: PA2G4/EBP1 Antibody (JE35-48) [NBP3-32288] - Western blot analysis of PA2G4/EBP1 on different lysates with Rabbit anti-PA2G4/EBP1 antibody (NBP3-32288) at 1/500 dilution. Lane 1: K562 cell lysate Lane 2: HEK-293 cell lysate Lane 3: PC-12 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 44 kDa Observed band size: 44 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32288) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32288_rabbit-pa2g4-ebp1-mab-je35-48-27820241633125.png "Western Blot: PA2G4/EBP1 Antibody (JE35-48) [NBP3-32288] - Western blot analysis of PA2G4/EBP1 on different lysates with Rabbit anti-PA2G4/EBP1 antibody (NBP3-32288) at 1/500 dilution. Lane 1: K562 cell lysate Lane 2: HEK-293 cell lysate Lane 3: PC-12 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 44 kDa Observed band size: 44 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32288) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: PA2G4/EBP1 Antibody (JE35-48) [NBP3-32288] - Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-PA2G4/EBP1 antibody (NBP3-32288) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32288) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32288_rabbit-pa2g4-ebp1-mab-je35-48-29820241435277.jpg)
![Immunocytochemistry/ Immunofluorescence: PA2G4/EBP1 Antibody (JE35-48) [NBP3-32288] - Immunocytochemistry analysis of 293 cells labeling PA2G4/EBP1 with Rabbit anti-PA2G4/EBP1 antibody (NBP3-32288) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-PA2G4/EBP1 antibody (NBP3-32288) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32288_rabbit-pa2g4-ebp1-mab-je35-48-2920241192382.jpg)
![Immunocytochemistry/Immunofluorescence: B7-H4 Antibody [NBP2-30536] - Immunofluorescent staining of human cell line MCF7 shows localization to plasma membrane & cell junctions.](http://images.novusbio.com/fullsize/B7-H4-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-30536-img0002.jpg "Immunocytochemistry/Immunofluorescence: B7-H4 Antibody [NBP2-30536] - Immunofluorescent staining of human cell line MCF7 shows localization to plasma membrane & cell junctions.")
![Immunohistochemistry-Paraffin: B7-H4 Antibody [NBP2-30536] - Staining of human breast shows moderate membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/B7-H4-Antibody-Immunohistochemistry-Paraffin-NBP2-30536-img0003.jpg)
![Immunohistochemistry-Paraffin: BTLA/CD272 Antibody [NBP2-49354] - Staining of human tonsil shows moderate positivity in lymphoid cells.](http://images.novusbio.com/fullsize/BTLA-CD272-Antibody-Immunohistochemistry-Paraffin-NBP2-49354-img0009.jpg "Immunohistochemistry-Paraffin: BTLA/CD272 Antibody [NBP2-49354] - Staining of human tonsil shows moderate positivity in lymphoid cells.")
![Immunohistochemistry-Paraffin: BTLA/CD272 Antibody [NBP2-49354] - Staining of human lung shows moderate cytoplasmic positivity in macrophages.](http://images.novusbio.com/fullsize/BTLA-CD272-Antibody-Immunohistochemistry-Paraffin-NBP2-49354-img0006.jpg)
![Immunohistochemistry-Paraffin: BTLA/CD272 Antibody [NBP2-49354] - Staining of human pancreas shows no cytoplasmic positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/BTLA-CD272-Antibody-Immunohistochemistry-Paraffin-NBP2-49354-img0007.jpg)
![Immunohistochemistry-Paraffin: BTLA/CD272 Antibody [NBP2-49354] - Staining of human small intestine shows moderate positivity in lymphoid cells.](http://images.novusbio.com/fullsize/BTLA-CD272-Antibody-Immunohistochemistry-Paraffin-NBP2-49354-img0008.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: BTLA/CD272 Antibody [NBP2-49354] - Staining in human tonsil and pancreas tissues . Corresponding BTLA RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/BTLA-CD272-Antibody-Immunohistochemistry-Paraffin-NBP2-49354-img0010.jpg)
![Western Blot: ARHGEF1 Antibody [NBP2-92963] - Analysis of extracts of various cell lines, using ARHGEF1 at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.](http://images.novusbio.com/fullsize/ARHGEF1-Antibody-Western-Blot-NBP2-92963-img0003.jpg "Western Blot: ARHGEF1 Antibody [NBP2-92963] - Analysis of extracts of various cell lines, using ARHGEF1 at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.")
![Immunohistochemistry-Paraffin: ARHGEF1 Antibody [NBP2-92963] - Human tonsil using ARHGEF1 antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/ARHGEF1-Antibody-Immunohistochemistry-Paraffin-NBP2-92963-img0001.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: ARHGEF1 Antibody [NBP2-92963] - Analysis of extracts from normal (control) and ARHGEF1 knockout (KO) 293T cells, using ARHGEF1 at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in](http://images.novusbio.com/fullsize/ARHGEF1-Antibody-Knockout-Validated-NBP2-92963-img0002.jpg)
![Western Blot: ILF3 Antibody [NBP1-71876] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. Affinity purified rabbit anti-NF90/NF110 antibody used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. NF90 and/or NF110 were also immunoprecipitated by rabbit anti-NF90/NF110 antibodies NBP1-71874 and NBP1-71875 as well as anti-NF110 antibody NBP1-71877, each of which recognizes a different epitope.](http://images.novusbio.com/fullsize/ILF3-Antibody-Western-Blot-NBP1-71876-img0001.jpg "Western Blot: ILF3 Antibody [NBP1-71876] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. Affinity purified rabbit anti-NF90/NF110 antibody used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. NF90 and/or NF110 were also immunoprecipitated by rabbit anti-NF90/NF110 antibodies NBP1-71874 and NBP1-71875 as well as anti-NF110 antibody NBP1-71877, each of which recognizes a different epitope.")
![Immunohistochemistry-Paraffin: ILF3 Antibody [NBP1-71876] - Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti- NF90/NF110 used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/ILF3-Antibody-Immunohistochemistry-Paraffin-NBP1-71876-img0002.jpg)
![Immunohistochemistry-Paraffin: PA2G4/EBP1 Antibody [NBP1-71839] - FFPE serial sections of human prostate carcinoma. Antibody: Affinity purified rabbit anti-EBP1, left image and , right image used at a dilution of 1:200 (1 ug/ml). Detection: DAB. Counterstain: hematoxylin (blue).](http://images.novusbio.com/fullsize/PA2G4-EBP1-Antibody-Immunohistochemistry-Paraffin-NBP1-71839-img0004.jpg "Immunohistochemistry-Paraffin: PA2G4/EBP1 Antibody [NBP1-71839] - FFPE serial sections of human prostate carcinoma. Antibody: Affinity purified rabbit anti-EBP1, left image and , right image used at a dilution of 1:200 (1 ug/ml). Detection: DAB. Counterstain: hematoxylin (blue).")
![PA2G4/EBP1 Antibody [NBP1-71839] - Whole cell lysate (10 ug) from HEK293T, HeLa, MCF-7, Jurkat, and Hep-G2 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-EBP1 antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/antibody/nbp1-71839_rabbit-polyclonal-pa2g4-ebp1-antibody-western-blot-115202394115..jpg)
![PA2G4/EBP1 Antibody [NBP1-71839] - Whole cell lysate (10 ug) from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-EBP1 antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/antibody/nbp1-71839_rabbit-polyclonal-pa2g4-ebp1-antibody-western-blot-115202394246..jpg)
![Western Blot: PA2G4/EBP1 Antibody [NBP1-71840] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T, 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. Affinity purified rabbit anti-EBP1 antibody used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. EBP1 was also immunoprecipitated by rabbit anti-EBP1 antibody NBP1-71839 which recognizes an upstream epitope.](http://images.novusbio.com/fullsize/PA2G4-EBP1-Antibody-Western-Blot-NBP1-71840-img0007.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: PA2G4/EBP1 Antibody [NBP1-71840] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T, 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. Affinity purified rabbit anti-EBP1 antibody used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. EBP1 was also immunoprecipitated by rabbit anti-EBP1 antibody NBP1-71839 which recognizes an upstream epitope.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunohistochemistry-Paraffin: PA2G4/EBP1 Antibody [NBP1-71840] - Human prostate carcinoma. Antibody: Affinity purified rabbit anti- EBP1 used at a dilution of 1:200 (1ug/ml). Detection: DAB. Counterstain: hematoxylin (blue).](http://images.novusbio.com/fullsize/PA2G4-EBP1-Antibody-Immunohistochemistry-NBP1-71840-img0008.jpg)
![Western Blot: ILF3 Antibody [NBP1-58226] - Titration: 0.2-1 ug/ml, Positive Control: 293T cell lysate.](http://images.novusbio.com/fullsize/ILF3-Antibody-Western-Blot-NBP1-58226-img0004.jpg "Western Blot: ILF3 Antibody [NBP1-58226] - Titration: 0.2-1 ug/ml, Positive Control: 293T cell lysate.")
![Immunohistochemistry: ILF3 Antibody [NBP1-58226] - Paraffin Embedded Tissue: Human Heart Cellular Data: Myocardial cells Antibody Concentration: 4.0 - 8.0 ug/ml Magnification: 400X](http://images.novusbio.com/fullsize/ILF3-Antibody-Immunohistochemistry-NBP1-58226-img0007.jpg)
![Immunohistochemistry-Paraffin: ILF3 Antibody [NBP1-58226] - Human Liver Tissue, antibody concentration 4-8ug/ml. Cells with positive label: Hepatocytes (indicated with arrows) 400X magnification.](http://images.novusbio.com/fullsize/ILF3-Antibody-Immunohistochemistry-Paraffin-NBP1-58226-img0005.jpg)
![Immunohistochemistry: ILF3 Antibody [NBP1-58226] - Paraffin Embedded Tissue: Human Heart cell Cellular Data: cardiac cell of renal tubule Antibody Concentration: 4.0 - 8.0 ug/ml Magnification: 400X](http://images.novusbio.com/fullsize/ILF3-Antibody-Immunohistochemistry-NBP1-58226-img0006.jpg)