| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-03736-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-04398-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35749-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52580-0.025mg | |||
![Western Blot: UbcH10/UBE2C Antibody [NB100-1084] - (2ug/ml) staining of HEK293 (A) and HeLa (B) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/UbcH10-UBE2C-Antibody-Western-Blot-NB100-1084-img0004.jpg "Western Blot: UbcH10/UBE2C Antibody [NB100-1084] - (2ug/ml) staining of HEK293 (A) and HeLa (B) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: UbcH10/UBE2C Antibody [NB100-1084] - Analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing cytoplasmic /Plasma Membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml).](http://images.novusbio.com/fullsize/UbcH10-UBE2C-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1084-img0003.jpg)
![Flow Cytometry: UbcH10/UBE2C Antibody [NB100-1084] - Analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/UbcH10-UBE2C-Antibody-Flow-Cytometry-NB100-1084-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: UbcH10/UBE2C Antibody [NB100-1084] - Analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml).](http://images.novusbio.com/fullsize/UbcH10-UBE2C-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1084-img0002.jpg)
![Western Blot: NDC80 Antibody [NB100-1100] - Staining (0.5 ug/ml) of Hela lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/NDC80-Antibody-Western-Blot-NB100-1100-img0003.jpg "Western Blot: NDC80 Antibody [NB100-1100] - Staining (0.5 ug/ml) of Hela lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: NDC80 Antibody [NB100-1100] - Staining of paraffin embedded Human Adrenal Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/NDC80-Antibody-Immunohistochemistry-Paraffin-NB100-1100-img0002.jpg)
![Immunohistochemistry-Paraffin: NDC80 Antibody [NB100-1100] - Staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/NDC80-Antibody-Immunohistochemistry-Paraffin-NB100-1100-img0001.jpg)
![Western Blot: M-CSF Antibody [NBP1-59425] - Sample Tissue: Human Fetal Liver Antibody Dilution: 1.0 ug/ml](http://images.novusbio.com/fullsize/M-CSF-Antibody-Western-Blot-NBP1-59425-img0008.jpg "Western Blot: M-CSF Antibody [NBP1-59425] - Sample Tissue: Human Fetal Liver Antibody Dilution: 1.0 ug/ml")
![Immunohistochemistry-Paraffin: M-CSF Antibody [NBP1-59425] - Human Placenta Tissue, 10 ug/ml](http://images.novusbio.com/fullsize/M-CSF-Antibody-Immunohistochemistry-Paraffin-NBP1-59425-img0004.jpg)
![Western Blot: M-CSF Antibody [NBP1-59425] - WB Suggested Anti-CSF1 Antibody Titration: 0.2-1 ug/ml ELISA Titer: 1:62500 Positive Control: COLO205 cell lysate](http://images.novusbio.com/fullsize/M-CSF-Antibody-Western-Blot-NBP1-59425-img0005.jpg)
![Western Blot: M-CSF Antibody [NBP1-59425] - Expressed in Human 721_B cells.](http://images.novusbio.com/fullsize/M-CSF-Antibody-Western-Blot-NBP1-59425-img0006.jpg)
![Western Blot: M-CSF Antibody [NBP1-59425] - Antibody Titration: 1 ug/ml Human heart.](http://images.novusbio.com/fullsize/M-CSF-Antibody-Western-Blot-NBP1-59425-img0007.jpg)
![Western Blot: NAP1L1 Antibody (4W8B6) [NBP3-16633] - Western blot analysis of extracts of various cell lines, using NAP1L1 Rabbit mAb (NBP3-16633) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/NAP1L1-Antibody-4W8B6-Western-Blot-NBP3-16633-img0004.jpg "Western Blot: NAP1L1 Antibody (4W8B6) [NBP3-16633] - Western blot analysis of extracts of various cell lines, using NAP1L1 Rabbit mAb (NBP3-16633) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Western Blot: NAP1L1 Antibody (4W8B6) [NBP3-16633] - Western blot analysis of extracts of various cell lines, using NAP1L1 Rabbit mAb (NBP3-16633) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.](http://images.novusbio.com/fullsize/NAP1L1-Antibody-4W8B6-Western-Blot-NBP3-16633-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: NAP1L1 Antibody (4W8B6) [NBP3-16633] - Immunofluorescence analysis of U-2 OS cells using NAP1L1 Rabbit mAb (NBP3-16633) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/NAP1L1-Antibody-4W8B6-Immunocytochemistry-Immunofluorescence-NBP3-16633-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: NAP1L1 Antibody (4W8B6) [NBP3-16633] - Immunofluorescence analysis of C6 cells using NAP1L1 Rabbit mAb (NBP3-16633) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/NAP1L1-Antibody-4W8B6-Immunocytochemistry-Immunofluorescence-NBP3-16633-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: NAP1L1 Antibody (4W8B6) [NBP3-16633] - Immunofluorescence analysis of NIH-3T3 cells using NAP1L1 Rabbit mAb (NBP3-16633) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/NAP1L1-Antibody-4W8B6-Immunocytochemistry-Immunofluorescence-NBP3-16633-img0002.jpg)
![Western Blot: Exostosin 1 Antibody [NBP3-03736] - Analysis of extracts of various cell lines, using Exostosin 1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.](http://images.novusbio.com/fullsize/Exostosin-1-Antibody-Western-Blot-NBP3-03736-img0002.jpg "Western Blot: Exostosin 1 Antibody [NBP3-03736] - Analysis of extracts of various cell lines, using Exostosin 1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.")
![Immunocytochemistry/Immunofluorescence: Exostosin 1 Antibody [NBP3-03736] -Analysis of NIH/3T3 cells using EXT1 Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/antibody/nbp3-03736_rabbit-polyclonal-exostosin-1-antibody-immunocytochemistry-immunofluorescence-1620238270..jpg)
![Immunocytochemistry/Immunofluorescence: Exostosin 1 Antibody [NBP3-03736] -Analysis of PC-12 cells using EXT1 Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/antibody/nbp3-03736_rabbit-polyclonal-exostosin-1-antibody-immunocytochemistry-immunofluorescence-16202382850..jpg)
![Immunocytochemistry/Immunofluorescence: Exostosin 1 Antibody [NBP3-03736] -Analysis of U2OS cells using EXT1 Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/antibody/nbp3-03736_rabbit-polyclonal-exostosin-1-antibody-immunocytochemistry-immunofluorescence-16202383040..jpg)
![Immunocytochemistry/ Immunofluorescence: Exostosin 1 Antibody - Azide and BSA Free [NBP3-03736] - Immunofluorescence analysis of HeLa cells using Exostosin 1 Rabbit pAb (A2030) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-03736_rabbit-polyclonal-exostosin-1-antibody-52202519313480.jpg)
![Western Blot: KLHL9 Antibody [NBP3-04398] - Analysis of extracts of 293T cells, using KLHL9 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit .](http://images.novusbio.com/fullsize/KLHL9-Antibody-Western-Blot-NBP3-04398-img0005.jpg "Western Blot: KLHL9 Antibody [NBP3-04398] - Analysis of extracts of 293T cells, using KLHL9 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit .")
![Immunohistochemistry-Paraffin: KLHL9 Antibody [NBP3-04398] - Paraffin-embedded mouse testis using KLHL9 antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/KLHL9-Antibody-Immunohistochemistry-Paraffin-NBP3-04398-img0004.jpg)
![Immunohistochemistry-Paraffin: KLHL9 Antibody [NBP3-04398] - Paraffin-embedded rat ovary using KLHL9 antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/KLHL9-Antibody-Immunohistochemistry-Paraffin-NBP3-04398-img0001.jpg)
![Immunohistochemistry-Paraffin: KLHL9 Antibody [NBP3-04398] - Paraffin-embedded rat heart using KLHL9 antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/KLHL9-Antibody-Immunohistochemistry-Paraffin-NBP3-04398-img0002.jpg)
![Immunohistochemistry-Paraffin: KLHL9 Antibody [NBP3-04398] - Paraffin-embedded human breast cancer using KLHL9 antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/KLHL9-Antibody-Immunohistochemistry-Paraffin-NBP3-04398-img0003.jpg)
![Western Blot: Cyclin B2 Antibody (1L9B9) [NBP3-16663] - Western blot analysis of extracts of Rat testis cells, using Cyclin B2 antibody (NBP3-16663) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/Cyclin-B2-Antibody-1L9B9-Western-Blot-NBP3-16663-img0003.jpg "Western Blot: Cyclin B2 Antibody (1L9B9) [NBP3-16663] - Western blot analysis of extracts of Rat testis cells, using Cyclin B2 antibody (NBP3-16663) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Immunocytochemistry/Immunofluorescence: Cyclin B2 Antibody (1L9B9) [NBP3-16663] - Immunofluorescence analysis of NIH-3T3 cells using Cyclin B2 Rabbit mAb (NBP3-16663) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Cyclin-B2-Antibody-1L9B9-Immunocytochemistry-Immunofluorescence-NBP3-16663-img0002.jpg)
![Immunohistochemistry-Paraffin: Cyclin B2 Antibody (1L9B9) [NBP3-16663] - Immunohistochemistry of paraffin-embedded human liver cancer using Cyclin B2 Rabbit mAb (NBP3-16663) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Cyclin-B2-Antibody-1L9B9-Immunohistochemistry-Paraffin-NBP3-16663-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: Cyclin B2 Antibody (1L9B9) [NBP3-16663] - Confocal imaging of NIH/3T3 cells using Cyclin B2 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo(R) 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16663_rabbit-cyclin-b2-mab-1l9b9-52202511392244.jpg)
![Western Blot: Septin-1 Antibody [NBP3-35749] - Western blot analysis of various lysates, using Septin-1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 20s.](http://images.novusbio.com/fullsize/nbp3-35749_rabbit-septin-1-pab-1012202411343345.jpg "Western Blot: Septin-1 Antibody [NBP3-35749] - Western blot analysis of various lysates, using Septin-1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 20s.")
![Western Blot: Septin-1 Antibody [NBP3-35749] - Western blot analysis of lysates from Rat spleen, using Septin-1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.](http://images.novusbio.com/fullsize/nbp3-35749_rabbit-septin-1-pab-101220241136056.jpg)
![Immunohistochemistry: Septin-1 Antibody [NBP3-35749] - Immunohistochemistry analysis of paraffin-embedded Rat spleen using Septin-1 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35749_rabbit-septin-1-pab-1012202411334077.jpg)
![Immunohistochemistry: Septin-1 Antibody [NBP3-35749] - Immunohistochemistry analysis of paraffin-embedded Mouse spleen using Septin-1 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35749_rabbit-septin-1-pab-1012202411343372.jpg)
![Immunohistochemistry: Septin-1 Antibody [NBP3-35749] - Immunohistochemistry analysis of paraffin-embedded Human appendix using Septin-1 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35749_rabbit-septin-1-pab-1012202411353057.jpg)
![Western Blot: SMC3 Antibody (8W9R7) [NBP3-15798] - Western blot analysis of extracts of various cell lines, using SMC3 Rabbit mAb (NBP3-15798) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/SMC3-Antibody-8W9R7-Western-Blot-NBP3-15798-img0004.jpg "Western Blot: SMC3 Antibody (8W9R7) [NBP3-15798] - Western blot analysis of extracts of various cell lines, using SMC3 Rabbit mAb (NBP3-15798) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunocytochemistry/Immunofluorescence: SMC3 Antibody (8W9R7) [NBP3-15798] - Immunofluorescence analysis of C6 using SMC3 antibody (NBP3-15798) at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/SMC3-Antibody-8W9R7-Immunocytochemistry-Immunofluorescence-NBP3-15798-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: SMC3 Antibody (8W9R7) [NBP3-15798] - Immunofluorescence analysis of U2OS using SMC3 antibody (NBP3-15798) at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/SMC3-Antibody-8W9R7-Immunocytochemistry-Immunofluorescence-NBP3-15798-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: SMC3 Antibody (8W9R7) [NBP3-15798] - Immunofluorescence analysis of NIH/3T3 using SMC3 antibody (NBP3-15798) at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/SMC3-Antibody-8W9R7-Immunocytochemistry-Immunofluorescence-NBP3-15798-img0002.jpg)
![Immunoprecipitation: SMC3 Antibody (8W9R7) [NBP3-15798] - Immunoprecipitation analysis of 300ug extracts of U-87MG cells using 3ug SMC3 antibody (NBP3-15798). Western blot was performed from the immunoprecipitate using SMC3 antibody (NBP3-15798) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/SMC3-Antibody-8W9R7-Immunoprecipitation-NBP3-15798-img0005.jpg)
![Western Blot: Cyclin D1 Antibody (7C8Z4) [NBP3-15627] - Western blot analysis of extracts of various cell lines, using Cyclin D1 antibody (NBP3-15627) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.](http://images.novusbio.com/fullsize/Cyclin-D1-Antibody-7C8Z4-Western-Blot-NBP3-15627-img0009.jpg "Western Blot: Cyclin D1 Antibody (7C8Z4) [NBP3-15627] - Western blot analysis of extracts of various cell lines, using Cyclin D1 antibody (NBP3-15627) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.")
![<b>Genetic Strategies Validation. </b>Western Blot: Cyclin D1 Antibody (7C8Z4) [NBP3-15627] - Western blot analysis of extracts from normal (control) and Cyclin D1 knockout (KO) HeLa cells, using Cyclin D1 antibody (NBP3-15627) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.](http://images.novusbio.com/fullsize/Cyclin-D1-Antibody-7C8Z4-Knockout-Validated-NBP3-15627-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Cyclin D1 Antibody (7C8Z4) [NBP3-15627] - Confocal imaging of NIH/3T3 cells using [KO Validated] Cyclin D1 Rabbit mAb (dilution 1:100) (Red). The cells were counterstained with a-Tubulin Mouse mAb (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100 x.](http://images.novusbio.com/fullsize/nbp3-15627_rabbit-cyclin-d1-mab-7c8z4-immunocytochemistry-immunofluorescence-2092023105125.jpg)
![Western Blot: UbcH10/UBE2C Antibody (1F5D3) [NBP2-52580] - Analysis using UBE2C mouse mAb against Hela (1), and Raji (2) cell lysate.](http://images.novusbio.com/fullsize/UbcH10-UBE2C-Antibody-1F5D3-Western-Blot-NBP2-52580-img0006.jpg "Western Blot: UbcH10/UBE2C Antibody (1F5D3) [NBP2-52580] - Analysis using UBE2C mouse mAb against Hela (1), and Raji (2) cell lysate.")
![Immunohistochemistry-Paraffin: UbcH10/UBE2C Antibody (1F5D3) [NBP2-52580] - Analysis of paraffin-embedded esophageal cancer tissues using UBE2C mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/UbcH10-UBE2C-Antibody-1F5D3-Immunohistochemistry-Paraffin-NBP2-52580-img0002.jpg)
![Western Blot: UbcH10/UBE2C Antibody (1F5D3) [NBP2-52580] - Analysis using UBE2C mAb against human UBE2C (AA: FULL(1-179)) recombinant protein. (Expected MW is 45.6 kDa)](http://images.novusbio.com/fullsize/UbcH10-UBE2C-Antibody-1F5D3-Western-Blot-NBP2-52580-img0003.jpg)
![Western Blot: UbcH10/UBE2C Antibody (1F5D3) [NBP2-52580] - Analysis using UBE2C mAb against HEK293 (1) and UBE2C (AA: FULL(1-179))-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/UbcH10-UBE2C-Antibody-1F5D3-Western-Blot-NBP2-52580-img0004.jpg)
![Immunohistochemistry-Paraffin: UbcH10/UBE2C Antibody (1F5D3) [NBP2-52580] - Analysis of paraffin-embedded liver cancer tissues using UBE2C mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/UbcH10-UBE2C-Antibody-1F5D3-Immunohistochemistry-Paraffin-NBP2-52580-img0001.jpg)
![ELISA: UbcH10/UBE2C Antibody (1F5D3) [NBP2-52580] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).](http://images.novusbio.com/fullsize/UbcH10-UBE2C-Antibody-1F5D3-ELISA-NBP2-52580-img0005.jpg)
![Western Blot: CDC25C Antibody (JE59-53) [NBP3-32173] - Western blot analysis of CDC25C on different lysates with Rabbit anti-CDC25C antibody (NBP3-32173) at 1/1,000 dilution. Lane 1: 293T cell lysate Lane 2: Caco-2 cell lysate Lane 3: HUVEC cell lysate Lane 4: HT-29 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 53 kDa Observed band size: 58 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32173) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32173_rabbit-cdc25c-mab-je59-53-27820241631628.jpg "Western Blot: CDC25C Antibody (JE59-53) [NBP3-32173] - Western blot analysis of CDC25C on different lysates with Rabbit anti-CDC25C antibody (NBP3-32173) at 1/1,000 dilution. Lane 1: 293T cell lysate Lane 2: Caco-2 cell lysate Lane 3: HUVEC cell lysate Lane 4: HT-29 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 53 kDa Observed band size: 58 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32173) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: CDC25C Antibody (JE59-53) [NBP3-32173] - Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-CDC25C antibody (NBP3-32173) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32173) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32173_rabbit-cdc25c-mab-je59-53-29820241420958.jpg)
![Immunocytochemistry/ Immunofluorescence: CDC25C Antibody (JE59-53) [NBP3-32173] - Immunocytochemistry analysis of HeLa cells labeling CDC25C with Rabbit anti-CDC25C antibody (NBP3-32173) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CDC25C antibody (NBP3-32173) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32173_rabbit-cdc25c-mab-je59-53-2920241023428.jpg)
![Flow Cytometry: CDC25C Antibody (JE59-53) [NBP3-32173] - Flow cytometric analysis of HeLa cells labeling CDC25C. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32173, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32173_rabbit-cdc25c-mab-je59-53-29202416453230.jpg)