| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50538-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90058-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-44309SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-42210SS | |||
| Azide and BSA Free | NBP2-80739 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-33321-0.1mg | |||
![Western Blot: p27/Kip1 Antibody (9R5B4) [NBP3-15662] - Analysis of extracts of various cell lines, using p27/Kip1 antibody (NBP3-15662) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/p27-Kip1-Antibody-9R5B4-Western-Blot-NBP3-15662-img0003.jpg "Western Blot: p27/Kip1 Antibody (9R5B4) [NBP3-15662] - Analysis of extracts of various cell lines, using p27/Kip1 antibody (NBP3-15662) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.")
![Immunohistochemistry-Paraffin: p27/Kip1 Antibody (9R5B4) [NBP3-15662] - Rat ovary using p27/Kip1 Rabbit mAb (NBP3-15662) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/p27-Kip1-Antibody-9R5B4-Immunohistochemistry-Paraffin-NBP3-15662-img0001.jpg)
![Immunohistochemistry: p27/Kip1 Antibody (9R5B4) [p27/Kip1] - Immunohistochemistry analysis of paraffin-embedded Human brain tissue using p27/Kip1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15662_rabbit-p27-kip1-mab-9r5b4-52202517315585.jpg)
![Immunohistochemistry: p27/Kip1 Antibody (9R5B4) [p27/Kip1] - Immunohistochemistry analysis of paraffin-embedded Human lung squamous carcinoma tissue using p27/Kip1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15662_rabbit-p27-kip1-mab-9r5b4-5220251734546.jpg)
![Immunohistochemistry: p27/Kip1 Antibody (9R5B4) [p27/Kip1] - Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using p27/Kip1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15662_rabbit-p27-kip1-mab-9r5b4-5220251734555.jpg)
![Immunohistochemistry: p27/Kip1 Antibody (9R5B4) [p27/Kip1] - Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using p27/Kip1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15662_rabbit-p27-kip1-mab-9r5b4-52202517333867.jpg)
![Immunohistochemistry: p27/Kip1 Antibody (9R5B4) [p27/Kip1] - Immunohistochemistry analysis of paraffin-embedded Human placenta tissue using p27/Kip1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15662_rabbit-p27-kip1-mab-9r5b4-52202517304988.jpg)
![Immunohistochemistry: p27/Kip1 Antibody (9R5B4) [p27/Kip1] - Immunohistochemistry analysis of paraffin-embedded Human spleen tissue using p27/Kip1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15662_rabbit-p27-kip1-mab-9r5b4-5220251731107.jpg)
![Immunohistochemistry: p27/Kip1 Antibody (9R5B4) [p27/Kip1] - Immunohistochemistry analysis of paraffin-embedded Human thyroid tissue using p27/Kip1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15662_rabbit-p27-kip1-mab-9r5b4-52202517363713.jpg)
![Immunohistochemistry: p27/Kip1 Antibody (9R5B4) [p27/Kip1] - Immunohistochemistry analysis of paraffin-embedded Human lung adenocarcinoma tissue using p27/Kip1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15662_rabbit-p27-kip1-mab-9r5b4-52202517311074.jpg)
![Western Blot: CDK4 Antibody (3G7Q7) [NBP3-15344] - Western blot analysis of extracts of MCF7 cells, using CDK4 Rabbit mAb (NBP3-15344) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/CDK4-Antibody-3G7Q7-Western-Blot-NBP3-15344-img0008.jpg "Western Blot: CDK4 Antibody (3G7Q7) [NBP3-15344] - Western blot analysis of extracts of MCF7 cells, using CDK4 Rabbit mAb (NBP3-15344) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Immunocytochemistry/Immunofluorescence: CDK4 Antibody (3G7Q7) [NBP3-15344] - Immunofluorescence analysis of HeLa cells using CDK4 Rabbit mAb (NBP3-15344) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/CDK4-Antibody-3G7Q7-Immunocytochemistry-Immunofluorescence-NBP3-15344-img0007.jpg)
![Immunohistochemistry-Paraffin: CDK4 Antibody (3G7Q7) [NBP3-15344] - Immunohistochemistry of paraffin-embedded rat ovary using [KO Validated] CDK4 Rabbit mAb (NBP3-15344) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/CDK4-Antibody-3G7Q7-Immunohistochemistry-Paraffin-NBP3-15344-img0006.jpg)
![Immunohistochemistry-Paraffin: CDK4 Antibody (3G7Q7) [NBP3-15344] - Immunohistochemistry of paraffin-embedded human breast cancer using [KO Validated] CDK4 Rabbit mAb (NBP3-15344) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/CDK4-Antibody-3G7Q7-Immunohistochemistry-Paraffin-NBP3-15344-img0001.jpg)
![Immunohistochemistry-Paraffin: CDK4 Antibody (3G7Q7) [NBP3-15344] - Immunohistochemistry of paraffin-embedded human colon carcinoma using [KO Validated] CDK4 Rabbit mAb (NBP3-15344) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/CDK4-Antibody-3G7Q7-Immunohistochemistry-Paraffin-NBP3-15344-img0002.jpg)
![Immunohistochemistry-Paraffin: CDK4 Antibody (3G7Q7) [NBP3-15344] - Immunohistochemistry of paraffin-embedded mouse lung using [KO Validated] CDK4 Rabbit mAb (NBP3-15344) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/CDK4-Antibody-3G7Q7-Immunohistochemistry-Paraffin-NBP3-15344-img0003.jpg)
![Immunohistochemistry-Paraffin: CDK4 Antibody (3G7Q7) [NBP3-15344] - Immunohistochemistry of paraffin-embedded mouse spleen using [KO Validated] CDK4 Rabbit mAb (NBP3-15344) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/CDK4-Antibody-3G7Q7-Immunohistochemistry-Paraffin-NBP3-15344-img0004.jpg)
![Immunohistochemistry-Paraffin: CDK4 Antibody (3G7Q7) [NBP3-15344] - Immunohistochemistry of paraffin-embedded rat lung using [KO Validated] CDK4 Rabbit mAb (NBP3-15344) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/CDK4-Antibody-3G7Q7-Immunohistochemistry-Paraffin-NBP3-15344-img0005.jpg)
![Western Blot: CDK4 Antibody (6N3M6) [CDK4] - Western blot analysis of various lysates, using CDK4 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-15344_rabbit-cdk4-mab-6n3m6-52202517162385.jpg)
![Immunohistochemistry: CDK4 Antibody (6N3M6) [CDK4] - Immunohistochemistry analysis of paraffin-embedded Human prostate tissue using [KO Validated] CDK4 Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15344_rabbit-cdk4-mab-6n3m6-52202517284930.jpg)
![Immunohistochemistry: CDK4 Antibody (6N3M6) [NBP3-15344] - Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using [KO Validated] CDK4 Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15344_rabbit-cdk4-mab-6n3m6-72202512435398.jpg)
![Immunohistochemistry: CDK4 Antibody (6N3M6) [NBP3-15344] - Immunohistochemistry analysis of paraffin-embedded Human cervix cancer tissue using [KO Validated] CDK4 Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15344_rabbit-cdk4-mab-6n3m6-7220251245317.jpg)
![Immunohistochemistry: CDK4 Antibody (6N3M6) [NBP3-15344] - Immunohistochemistry analysis of paraffin-embedded Human cervix cancer tissue using [KO Validated] CDK4 Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15344_rabbit-cdk4-mab-6n3m6-7220251245373.jpg)
![Immunohistochemistry: CDK4 Antibody (6N3M6) [NBP3-15344] - Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using [KO Validated] CDK4 Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15344_rabbit-cdk4-mab-6n3m6-7220251245387.jpg)
![Western Blot: Smad2 Antibody (5G7) [NBP2-37580] - Western blot analysis using SMAD2 mAb against HEK293 (1) and SMAD2(AA: 20-254)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Smad2-Antibody-5G7-Western-Blot-NBP2-37580-img0003.jpg "Western Blot: Smad2 Antibody (5G7) [NBP2-37580] - Western blot analysis using SMAD2 mAb against HEK293 (1) and SMAD2(AA: 20-254)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunocytochemistry/Immunofluorescence: Smad2 Antibody (5G7) [NBP2-37580] - Immunofluorescence analysis of U251 cells using SMAD2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/Smad2-Antibody-5G7-Immunocytochemistry-Immunofluorescence-NBP2-37580-img0004.jpg)
![Immunohistochemistry: Smad2 Antibody (5G7) [NBP2-37580] - Immunohistochemical analysis of paraffin-embedded human liver cancer tissues using SMAD2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Smad2-Antibody-5G7-Immunohistochemistry-NBP2-37580-img0007.jpg)
![Flow Cytometry: Smad2 Antibody (5G7) [NBP2-37580] - Flow cytometric analysis of NIH/3T3 cells using SMAD2 mouse mAb (blue) and negative control (red).](http://images.novusbio.com/fullsize/Smad2-Antibody-5G7-Flow-Cytometry-NBP2-37580-img0005.jpg)
![Western Blot: Smad2 Antibody (5G7) [NBP2-37580] - Western blot analysis using SMAD2 mAb against human SMAD2 (AA: 20-254) recombinant protein. (Expected MW is 52.2 kDa)](http://images.novusbio.com/fullsize/Smad2-Antibody-5G7-Western-Blot-NBP2-37580-img0002.jpg)
![Immunohistochemistry: Smad2 Antibody (5G7) [NBP2-37580] - Immunohistochemical analysis of paraffin-embedded human cerebellum tissues using SMAD2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Smad2-Antibody-5G7-Immunohistochemistry-NBP2-37580-img0006.jpg)
![ELISA: Smad2 Antibody (5G7) [NBP2-37580] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/Smad2-Antibody-5G7-ELISA-NBP2-37580-img0001.jpg)
![Western Blot: p53 Antibody (Pab DO-1) [NBP2-50538] - B) Western Blot analyses of RPMI-8402 and NALM-6 cell lines treated for 24 h with AZD-1775 (185 nM) and PF-00477736 (25 and 250 nM respectively). B-actin was used for loading normalization. For relative quantification of each protein see Figure S3A and for whole western blot images see Figure S6. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/2072-6694/11/11/1654) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/p53-Antibody-Pab-DO-1-Western-Blot-NBP2-50538-img0001.jpg "Western Blot: p53 Antibody (Pab DO-1) [NBP2-50538] - B) Western Blot analyses of RPMI-8402 and NALM-6 cell lines treated for 24 h with AZD-1775 (185 nM) and PF-00477736 (25 and 250 nM respectively). B-actin was used for loading normalization. For relative quantification of each protein see Figure S3A and for whole western blot images see Figure S6. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/2072-6694/11/11/1654) licensed under a CC-BY license.")
![Western Blot: p53 Antibody (Pab DO-1) - BSA Free [NBP2-50538] - DCQ reduces HIF-1 alpha through different mechanisms in MCF-7 & MDA-MB-231. (A) MCF-7 cells were transfected with siRNA against p53 & ctrl siRNA (scrambled sequence) using lipofectamine 2000. After 24 hours, cells were treated with DCQ (5 μM) for 6 hours under hypoxia. Whole cell lysates were prepared, & blots were probed against indicated antibodies. (B) MCF-7 cells were transfected with siRNA against p53 & ctrl siRNA (scrambled sequence) using lipofectamine 2000. Transfected cells were treated with DCQ (5 μM) for 6 hours under hypoxia. The extent of DNA fragmentation was determined by TUNEL assay 24 hours later using flow cytometry. One-way ANOVA was used to compare DCQ-treated versus control & statistical significance of p < 0.05 is indicated by *. (C) Whole cell lysates were prepared after pretreating MCF-7 & MDA-MB-231 with the proteasome inhibitor MG132 (3 μM) then treated with DCQ, & blots were probed for HIF-1 alpha . Results are from three independent experiments. (D) Whole cell lysates of MCF-7 were prepared after 6 hours of exposure to DCQ (5 μM) under normoxia or hypoxia, & blots were probed for p-AKT, mTOR, p-mTOR & GAPDH. Image collected & cropped by CiteAb from the following publication (//molecular-cancer.biomedcentral.com/articles/10.1186/1476-4598-13-12), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-50538_mouse-p53-mab-pab-do-1-bsa-free-310202416114753.jpg)
![Western Blot: p53 Antibody (Pab DO-1) - BSA Free [NBP2-50538] - DCQ induces DNA damage & apoptosis via reactive oxygen species. (A) Whole cell lysates of MCF-7 & MDA-MB-231 were prepared after 6 hours of exposure to DCQ (5 μM) under normoxia or hypoxia, & blots were probed for p-H2AX, p53, p-p53, p21, HIF-1 alpha & GAPDH. Results are representative of three independent experiments. (B) MDA-MB-231 & MCF-7 cells were pretreated with 1 mM Vitamin E or DTT for 2 hours followed by 25 min incubation with 10 μM CM-H2DCFDA dye. Cells were washed with PBS & treated with DCQ for 1 hour under normoxia or hypoxia, after which cells were harvested & the amount of DCF fluorescence was analyzed by flow cytometry. Each percentage is the average ± SE of three independent experiments. (C) MDA-MB-231 & MCF-7 cells were pretreated with 1 mM Vitamin E or DTT for 2 hours followed by 6 hours treatment with DCQ under normoxia or hypoxia. After 24 hours, the extent of DNA fragmentation was determined by TUNEL assay & measured by flow cytometry. One-way ANOVA was used to compare DCQ-treated versus control & statistical significance of p < 0.05 is indicated by *. (D) Whole cell lysates of MCF-7 & MDA-MB-231 were prepared after 6 hours of exposure to DCQ (5 μM) under normoxia or hypoxia, & blots were probed for HIF-1 alpha & GAPDH. Results are representative of three independent experiments. Image collected & cropped by CiteAb from the following publication (//molecular-cancer.biomedcentral.com/articles/10.1186/1476-4598-13-12), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-50538_mouse-p53-mab-pab-do-1-bsa-free-31020241612246.jpg)
![Flow Cytometry: Mouse Monoclonal p53 Antibody (Pab DO-1) [NBP2-50538] - P53 expression in monocyte subset of Human PBMCs post IFN gamma treatment. Image from a verified customer review. The customer used the Alexa Fluor® 647-conjugated version of NBP2-50538, which is listed under NBP2-50538AF647.](http://images.novusbio.com/fullsize/antibody/nbp2-50538_mouse-p53-mab-pab-do-1-bsa-free-flow-cytometry-152025141858..png)
![Western Blot: Galectin-3BP/MAC-2BP/LGALS3BP Antibody (OTI6B7) - Azide and BSA Free [NBP2-71125] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LGALS3BP (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LGALS3BP.](http://images.novusbio.com/fullsize/Galectin-3BP-MAC-2BP-LGALS3BP-Antibody-OTI6B7-Azide-and-BSA-Free-Western-Blot-NBP2-71125-img0002.jpg "Western Blot: Galectin-3BP/MAC-2BP/LGALS3BP Antibody (OTI6B7) - Azide and BSA Free [NBP2-71125] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LGALS3BP (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LGALS3BP.")
![Immunohistochemistry: Galectin-3BP/MAC-2BP/LGALS3BP Antibody (OTI6B7) - Azide and BSA Free [NBP2-71125] - Staining of paraffin-embedded Human pancreas tissue using anti-LGALS3BP mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Galectin-3BP-MAC-2BP-LGALS3BP-Antibody-OTI6B7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71125-img0007.jpg)
![Flow Cytometry: Galectin-3BP/MAC-2BP/LGALS3BP Antibody (OTI6B7) - Azide and BSA Free [NBP2-71125] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-LGALS3BP antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/Galectin-3BP-MAC-2BP-LGALS3BP-Antibody-OTI6B7-Azide-and-BSA-Free-Flow-Cytometry-NBP2-71125-img0003.jpg)
![Western Blot: Galectin-3BP/MAC-2BP/LGALS3BP Antibody (OTI6B7) - Azide and BSA Free [NBP2-71125] - Analysis of extracts (35ug) from 9 different cell lines by using anti-LGALS3BP monoclonal antibody.](http://images.novusbio.com/fullsize/Galectin-3BP-MAC-2BP-LGALS3BP-Antibody-OTI6B7-Azide-and-BSA-Free-Western-Blot-NBP2-71125-img0001.jpg)
![Immunohistochemistry: Galectin-3BP/MAC-2BP/LGALS3BP Antibody (OTI6B7) - Azide and BSA Free [NBP2-71125] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-LGALS3BP mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Galectin-3BP-MAC-2BP-LGALS3BP-Antibody-OTI6B7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71125-img0004.jpg)
![Immunohistochemistry: Galectin-3BP/MAC-2BP/LGALS3BP Antibody (OTI6B7) - Azide and BSA Free [NBP2-71125] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-LGALS3BP mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Galectin-3BP-MAC-2BP-LGALS3BP-Antibody-OTI6B7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71125-img0005.jpg)
![Immunohistochemistry: Galectin-3BP/MAC-2BP/LGALS3BP Antibody (OTI6B7) - Azide and BSA Free [NBP2-71125] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-LGALS3BP mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Galectin-3BP-MAC-2BP-LGALS3BP-Antibody-OTI6B7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71125-img0006.jpg)
![Immunohistochemistry: Galectin-3BP/MAC-2BP/LGALS3BP Antibody (OTI6B7) - Azide and BSA Free [NBP2-71125] - Staining of paraffin-embedded Human prostate tissue using anti-LGALS3BP mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Galectin-3BP-MAC-2BP-LGALS3BP-Antibody-OTI6B7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71125-img0008.jpg)
![Western Blot: FRA-1/FOSL1 Antibody (OTI12F9) - Azide and BSA Free [NBP2-71351] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FOSL1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FOSL1.](http://images.novusbio.com/fullsize/FRA-1-FOSL1-Antibody-OTI12F9-Azide-and-BSA-Free-Western-Blot-NBP2-71351-img0001.jpg "Western Blot: FRA-1/FOSL1 Antibody (OTI12F9) - Azide and BSA Free [NBP2-71351] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FOSL1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FOSL1.")
![Immunohistochemistry: FRA-1/FOSL1 Antibody (OTI12F9) - Azide and BSA Free [NBP2-71351] - Staining of paraffin-embedded Ovary tissue using anti-FOSL1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/FRA-1-FOSL1-Antibody-OTI12F9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71351-img0009.jpg)
![Immunohistochemistry: FRA-1/FOSL1 Antibody (OTI12F9) - Azide and BSA Free [NBP2-71351] - Carcinoma of bladder tissue.](http://images.novusbio.com/fullsize/FRA-1-FOSL1-Antibody-OTI12F9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71351-img0002.jpg)
![Immunohistochemistry: FRA-1/FOSL1 Antibody (OTI12F9) - Azide and BSA Free [NBP2-71351] - Staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-FOSL1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/FRA-1-FOSL1-Antibody-OTI12F9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71351-img0003.jpg)
![Immunohistochemistry: FRA-1/FOSL1 Antibody (OTI12F9) - Azide and BSA Free [NBP2-71351] - Staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-FOSL1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/FRA-1-FOSL1-Antibody-OTI12F9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71351-img0004.jpg)
![Immunohistochemistry: FRA-1/FOSL1 Antibody (OTI12F9) - Azide and BSA Free [NBP2-71351] - Staining of paraffin-embedded colon tissue using anti-FOSL1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/FRA-1-FOSL1-Antibody-OTI12F9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71351-img0005.jpg)
![Immunohistochemistry: FRA-1/FOSL1 Antibody (OTI12F9) - Azide and BSA Free [NBP2-71351] - Staining of paraffin-embedded endometrium tissue using anti-FOSL1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/FRA-1-FOSL1-Antibody-OTI12F9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71351-img0006.jpg)
![Immunohistochemistry: FRA-1/FOSL1 Antibody (OTI12F9) - Azide and BSA Free [NBP2-71351] - Staining of paraffin-embedded Kidney tissue using anti-FOSL1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/FRA-1-FOSL1-Antibody-OTI12F9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71351-img0007.jpg)
![Immunohistochemistry: FRA-1/FOSL1 Antibody (OTI12F9) - Azide and BSA Free [NBP2-71351] - Staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-FOSL1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/FRA-1-FOSL1-Antibody-OTI12F9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71351-img0008.jpg)
![Immunohistochemistry: FRA-1/FOSL1 Antibody (OTI12F9) - Azide and BSA Free [NBP2-71351] - Staining of paraffin-embedded prostate tissue using anti-FOSL1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/FRA-1-FOSL1-Antibody-OTI12F9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71351-img0010.jpg)
![Western Blot: ER alpha/NR3A1 Antibody (OTI1B1) - Azide and BSA Free [NBP2-70687] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Estrogen Receptor alpha (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with ant](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-OTI1B1-Azide-and-BSA-Free-Western-Blot-NBP2-70687-img0001.jpg "Western Blot: ER alpha/NR3A1 Antibody (OTI1B1) - Azide and BSA Free [NBP2-70687] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Estrogen Receptor alpha (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with ant")
![Immunohistochemistry: ER alpha/NR3A1 Antibody (OTI1B1) - Azide and BSA Free [NBP2-70687] - Staining of paraffin-embedded Human prostate tissue using anti-Estrogen Receptor alpha mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-OTI1B1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70687-img0008.jpg)
![Immunohistochemistry: ER alpha/NR3A1 Antibody (OTI1B1) - Azide and BSA Free [NBP2-70687] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-Estrogen Receptor alpha mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-OTI1B1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70687-img0002.jpg)
![Immunohistochemistry: ER alpha/NR3A1 Antibody (OTI1B1) - Azide and BSA Free [NBP2-70687] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-Estrogen Receptor alpha mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-OTI1B1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70687-img0003.jpg)
![Immunohistochemistry: ER alpha/NR3A1 Antibody (OTI1B1) - Azide and BSA Free [NBP2-70687] - Staining of paraffin-embedded Human breast tissue using anti-Estrogen Receptor alpha mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-OTI1B1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70687-img0004.jpg)
![Immunohistochemistry: ER alpha/NR3A1 Antibody (OTI1B1) - Azide and BSA Free [NBP2-70687] - Staining of paraffin-embedded Human Kidney tissue using anti-Estrogen Receptor alpha mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-OTI1B1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70687-img0005.jpg)
![Immunohistochemistry: ER alpha/NR3A1 Antibody (OTI1B1) - Azide and BSA Free [NBP2-70687] - Staining of paraffin-embedded Human lymphoma tissue using anti-Estrogen Receptor alpha mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-OTI1B1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70687-img0006.jpg)
![Immunohistochemistry: ER alpha/NR3A1 Antibody (OTI1B1) - Azide and BSA Free [NBP2-70687] - Staining of paraffin-embedded Human pancreas tissue using anti-Estrogen Receptor alpha mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-OTI1B1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70687-img0007.jpg)
![Immunohistochemistry: ER alpha/NR3A1 Antibody (OTI1B1) - Azide and BSA Free [NBP2-70687] - Staining of paraffin-embedded Human tonsil using anti-Estrogen Receptor alpha mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-OTI1B1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70687-img0009.jpg)
![Western Blot: MSPR/Ron Antibody (SD2006) [NBP2-67814] - Analysis of RON on AGS cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/MSPR-Ron-Antibody-SD2006-Western-Blot-NBP2-67814-img0007.jpg "Western Blot: MSPR/Ron Antibody (SD2006) [NBP2-67814] - Analysis of RON on AGS cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: MSPR/Ron Antibody (SD2006) [NBP2-67814] - Staining RON in AGS cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/MSPR-Ron-Antibody-SD2006-Immunocytochemistry-Immunofluorescence-NBP2-67814-img0006.jpg)
![Immunohistochemistry-Paraffin: MSPR/Ron Antibody (SD2006) [NBP2-67814] - Analysis of paraffin-embedded human lung cancer tissue using anti-RON antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/MSPR-Ron-Antibody-SD2006-Immunohistochemistry-Paraffin-NBP2-67814-img0005.jpg)
![Flow Cytometry: MSPR/Ron Antibody (SD2006) [NBP2-67814] - Analysis of THP-1 cells with RON antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody](http://images.novusbio.com/fullsize/MSPR-Ron-Antibody-SD2006-Flow-Cytometry-NBP2-67814-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: MSPR/Ron Antibody (SD2006) [NBP2-67814] - Staining RON in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/MSPR-Ron-Antibody-SD2006-Immunocytochemistry-Immunofluorescence-NBP2-67814-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: MSPR/Ron Antibody (SD2006) [NBP2-67814] - Staining RON in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/MSPR-Ron-Antibody-SD2006-Immunocytochemistry-Immunofluorescence-NBP2-67814-img0003.jpg)
![Immunohistochemistry-Paraffin: MSPR/Ron Antibody (SD2006) [NBP2-67814] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-RON antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/MSPR-Ron-Antibody-SD2006-Immunohistochemistry-Paraffin-NBP2-67814-img0004.jpg)
![Immunohistochemistry-Paraffin: PCNA Antibody (144) [NBP2-90058] - Staining of human PCNA in human lymph node with rabbit monoclonal antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/PCNA-Antibody-144-Immunohistochemistry-Paraffin-NBP2-90058-img0004.jpg "Immunohistochemistry-Paraffin: PCNA Antibody (144) [NBP2-90058] - Staining of human PCNA in human lymph node with rabbit monoclonal antibody at 1:200 dilution.")
![Immunohistochemistry-Paraffin: PCNA Antibody (144) [NBP2-90058] - Staining of human PCNA in human breast carcinoma with rabbit monoclonal antibody at 1:200).](http://images.novusbio.com/fullsize/PCNA-Antibody-144-Immunohistochemistry-Paraffin-NBP2-90058-img0001.jpg)
![Immunohistochemistry-Paraffin: PCNA Antibody (144) [NBP2-90058] - Staining of human PCNA in human liver with rabbit monoclonal antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/PCNA-Antibody-144-Immunohistochemistry-Paraffin-NBP2-90058-img0002.jpg)
![Immunohistochemistry-Paraffin: PCNA Antibody (144) [NBP2-90058] - Staining of human PCNA in human colon carcinoma with rabbit monoclonal antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/PCNA-Antibody-144-Immunohistochemistry-Paraffin-NBP2-90058-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: HGF R/c-MET Antibody (1G7NB) - Azide Free [NBP2-44309] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with c-MET (1G7NB) at a 1:100 dilution overnight at 4 degrees Celsius and detected with Dylight 488 (Green). Actin was detected using Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were detected with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/HGF-R-c-MET-Antibody-1G7NB-Azide-Free-Immunocytochemistry-Immunofluorescence-NBP2-44309-img0003.jpg "Immunocytochemistry/Immunofluorescence: HGF R/c-MET Antibody (1G7NB) - Azide Free [NBP2-44309] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with c-MET (1G7NB) at a 1:100 dilution overnight at 4 degrees Celsius and detected with Dylight 488 (Green). Actin was detected using Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were detected with DAPI (Blue). Cells were imaged using a 40X objective.")
![Immunohistochemistry-Paraffin: HGF R/c-MET Antibody (1G7NB) - Azide Free [NBP2-44309] - IHC analysis of HGF R/c-Met (1G7NB) in human liver cancer.](http://images.novusbio.com/fullsize/HGF-R-c-MET-Antibody-1G7NB-Azide-Free-Immunohistochemistry-Paraffin-NBP2-44309-img0004.jpg)
![Flow (Intracellular): HGF R/c-MET Antibody (1G7NB) [Azide Free] [NBP2-44309] - c-MET antibody was tested in HeLa cells (1 x 10^6 cells/ml). After fixation and permeabilization, cells were stained using the anti-c-MET antibody (clone 1G7NB) at a 1:1000 dilution. Signal was detected using a Goat anti-Mouse Dylight 488 secondary (blue peak). Shown with secondary control (orange peak). Data was acquired on BD FACSCalibur.](http://images.novusbio.com/fullsize/HGF-R-c-MET-Antibody-1G7NB-Azide-Free-Flow-Intracellular-NBP2-44309-img0002.jpg)
![Western Blot: Flt-3/Flk-2/CD135 Antibody (7E8.2C8) [NBP2-42210] - WB analysis of lysates from (A) Human Embryonic Kidney 293 / HEK293 and (B) Human Embryonic carcinoma NCCIT cell lines using Flt-3/Flk-2/CD135 antibody (clone 7E8.2C8) at 2ug/ml concentration. The antibody detected single specific bands in both samples at the expected molecular weight position.](http://images.novusbio.com/fullsize/Flt-3-Flk-2-CD135-Antibody-7E8-2C8-Western-Blot-NBP2-42210-img0001.jpg "Western Blot: Flt-3/Flk-2/CD135 Antibody (7E8.2C8) [NBP2-42210] - WB analysis of lysates from (A) Human Embryonic Kidney 293 / HEK293 and (B) Human Embryonic carcinoma NCCIT cell lines using Flt-3/Flk-2/CD135 antibody (clone 7E8.2C8) at 2ug/ml concentration. The antibody detected single specific bands in both samples at the expected molecular weight position.")
![Immunohistochemistry-Paraffin: Flt-3/Flk-2/CD135 Antibody (7E8.2C8) [NBP2-42210] - IHC analysis of a formalin fixed paraffin embedded tissue section of human liver cancer using Flt-3/Flk-2/CD135 antibody (clone 7E2.2C8) at 15 ug/ml concentration. The antibody generated specific cytoplasmic immunostaining of FLT3 in the cancerous cells while the tumor stroma was largely negative for the staining.](http://images.novusbio.com/fullsize/Flt-3-Flk-2-CD135-Antibody-7E8-2C8-Immunohistochemistry-Paraffin-NBP2-42210-img0003.jpg)
![Flow Cytometry: Flt-3/Flk-2/CD135 Antibody (7E8.2C8) [NBP2-42210] - HEK293 cells were stained (surface) with Flt-3/Flk-2/CD135 antibody (clone: 7E8.2C8; red) or isotype control (mouse IgG2 lambda; blue) and positive staining observed using PE conjugated mouse anti-IgG(H+L) secondary antibody. Live cells (PPI negative) were gated for analysis](http://images.novusbio.com/fullsize/Flt-3-Flk-2-CD135-Antibody-7E8-2C8-Flow-Cytometry-NBP2-42210-img0002.jpg)
![Western Blot: ER alpha/NR3A1 Antibody (ER506) [NBP2-33320] - Western Blot Analysis of human MCF-7 cell lysate using ER alpha/NR3A1 Antibody (ER506).](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-ER506-Western-Blot-NBP2-33320-img0017.jpg "Western Blot: ER alpha/NR3A1 Antibody (ER506) [NBP2-33320] - Western Blot Analysis of human MCF-7 cell lysate using ER alpha/NR3A1 Antibody (ER506).")
![Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody (ER506) [NBP2-33320] - Formalin-fixed, paraffin-embedded human Endometrium stained with ER alpha/NR3A1 Antibody (ER506).](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-ER506-Immunohistochemistry-Paraffin-NBP2-33320-img0015.jpg)
![Flow Cytometry: ER alpha/NR3A1 Antibody (ER506) [NBP2-33320] - Flow Cytometry for human ER-alpha on MCF-7 cells. Black: cells alone; Green: Isotype Control; Red: PE-labeled ER alpha/NR3A1 antibody (ER506).](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-ER506-Flow-Cytometry-NBP2-33320-img0018.jpg)
![Simple Western: ER alpha/NR3A1 Antibody (ER506) [NBP2-33320] - Simple Western lane view shows a specific band for Estrogen Receptor in 0.2 mg/ml of MCF-7 (left) and T47D (right) lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-ER506-Simple-Western-NBP2-33320-img0007.jpg)
![Simple Western: ER alpha/NR3A1 Antibody (ER506) [NBP2-33320] - Electropherogram image of the corresponding Simple Western lane. Estrogen Receptor antibody was used at 10 ug/ml dilution of MCF-7 and T47D lysates(s) respectively.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-ER506-Simple-Western-NBP2-33320-img0009.jpg)
![Protein Array: ER alpha/NR3A1 Antibody (ER506) [NBP2-33320] - Analysis of Protein Array containing more than 19,000 full-length human proteins using ER alpha/NR3A1 Antibody (ER506). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD?s) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD?s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-ER506-Protein-Array-NBP2-33320-img0016.jpg)