| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-24592-100ul | |||
| BSA Free | NBP3-24592-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-86860-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-73184 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-92908-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-93864-0.02ml | |||
![Immunohistochemistry-Paraffin: LATS1 Antibody [NBP1-86860] - Staining of human rectum shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/LATS1-Antibody-Immunohistochemistry-Paraffin-NBP1-86860-img0011.jpg "Immunohistochemistry-Paraffin: LATS1 Antibody [NBP1-86860] - Staining of human rectum shows moderate cytoplasmic positivity in glandular cells.")
![Immunohistochemistry-Paraffin: LATS1 Antibody [NBP1-86860] - Staining of human fallopian tube shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/LATS1-Antibody-Immunohistochemistry-Paraffin-NBP1-86860-img0008.jpg)
![Immunohistochemistry-Paraffin: LATS1 Antibody [NBP1-86860] - Staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/LATS1-Antibody-Immunohistochemistry-Paraffin-NBP1-86860-img0009.jpg)
![Immunohistochemistry-Paraffin: LATS1 Antibody [NBP1-86860] - Staining of human prostate shows strong cytoplasmic positivity in smooth muscles and glandular cells.](http://images.novusbio.com/fullsize/LATS1-Antibody-Immunohistochemistry-Paraffin-NBP1-86860-img0010.jpg)
![Western Blot: TOR/mTOR [p Ser2448] Antibody [NBP3-13144] - Untreated (-) and treated (+) 293T whole cell extracts (60 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with TOR/mTOR (phospho Ser2448) antibody (NBP3-13144) diluted at 1:1000.](http://images.novusbio.com/fullsize/TOR-mTOR-[p-Ser2448]-Antibody-Western-Blot-NBP3-13144-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: TOR/mTOR [p Ser2448] Antibody [NBP3-13144] - Untreated (-) and treated (+) 293T whole cell extracts (60 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with TOR/mTOR (phospho Ser2448) antibody (NBP3-13144) diluted at 1:1000.")
![<b>Biological Strategies Validation. </b>Western Blot: TOR/mTOR [p Ser2448] Antibody [NBP3-13144] - Untreated (-) and treated (+) NIH-3T3 whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with TOR/mTOR (phospho Ser2448) antibody (NBP3-13144) diluted at 1:1000. The signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/TOR-mTOR-[p-Ser2448]-Antibody-Western-Blot-NBP3-13144-img0002.jpg)
![ICC/IF: TOR/mTOR [p Ser2448] Antibody [NBP3-13144] -HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: mTOR (phospho Ser2448) stained by mTOR (phospho Ser2448) antibody diluted at 1:500.Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/antibody/nbp3-13144_rabbit-tor-mtor-p-ser-2448-pab-immunocytochemistry-immunofluorescence-126202482926..jpg)
![Western Blot: p15INK4b/CDKN2B Antibody (OTI3B6) [NBP2-45603] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDKN2B.](http://images.novusbio.com/fullsize/p15INK4b-CDKN2B-Antibody-OTI3B6-Western-Blot-NBP2-45603-img0005.jpg "Western Blot: p15INK4b/CDKN2B Antibody (OTI3B6) [NBP2-45603] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDKN2B.")
![Immunohistochemistry: p15INK4b/CDKN2B Antibody (OTI3B6) [NBP2-45603] - Analysis of Adenocarcinoma of Human endometrium tissue.](http://images.novusbio.com/fullsize/p15INK4b-CDKN2B-Antibody-OTI3B6-Immunohistochemistry-NBP2-45603-img0004.jpg)
![Flow Cytometry: p15INK4b/CDKN2B Antibody (OTI3B6) [NBP2-45603] - Analysis of HEK293T cells transfected with either overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by CDKN2B antibody.](http://images.novusbio.com/fullsize/p15INK4b-CDKN2B-Antibody-OTI3B6-Flow-Cytometry-NBP2-45603-img0001.jpg)
![Immunohistochemistry: p15INK4b/CDKN2B Antibody (OTI3B6) [NBP2-45603] - Analysis of Human pancreas tissue.](http://images.novusbio.com/fullsize/p15INK4b-CDKN2B-Antibody-OTI3B6-Immunohistochemistry-NBP2-45603-img0002.jpg)
![Immunohistochemistry: p15INK4b/CDKN2B Antibody (OTI3B6) [NBP2-45603] - Analysis of Carcinoma of Human thyroid tissue.](http://images.novusbio.com/fullsize/p15INK4b-CDKN2B-Antibody-OTI3B6-Immunohistochemistry-NBP2-45603-img0003.jpg)
![Western Blot: p27/Kip1 Antibody [NB100-2816] - Human Prostate lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/p27-Kip1-Antibody-Western-Blot-NB100-2816-img0005.jpg "Western Blot: p27/Kip1 Antibody [NB100-2816] - Human Prostate lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: p27/Kip1 Antibody [NB100-2816] - Staining of paraffin embedded Human Spleen. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/p27-Kip1-Antibody-Immunohistochemistry-Paraffin-NB100-2816-img0006.jpg)
![Immunohistochemistry-Paraffin: p27/Kip1 Antibody [NB100-2816] - Staining of paraffin embedded Human Prostate. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/p27-Kip1-Antibody-Immunohistochemistry-Paraffin-NB100-2816-img0004.jpg)
![Western Blot: CIRBP Antibody (3V9S7) [NBP3-15745] - Analysis of extracts of various cell lines, using CIRBP antibody (NBP3-15745) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/CIRBP-Antibody-3V9S7-Western-Blot-NBP3-15745-img0005.jpg "Western Blot: CIRBP Antibody (3V9S7) [NBP3-15745] - Analysis of extracts of various cell lines, using CIRBP antibody (NBP3-15745) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.")
![Immunohistochemistry: CIRBP Antibody (3V9S7) [NBP3-15745] - Immunohistochemistry analysis of CIRBP in paraffin-embedded human lung tissue using CIRBP Rabbit mAb at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15745_rabbit-cirbp-mab-3v9s7-8220252010458.jpg)
![Immunocytochemistry/ Immunofluorescence: CIRBP Antibody (3V9S7) [NBP3-15745] - Confocal imaging of paraffin-embedded Human breast cancer tissue using CIRBP Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-15745_rabbit-cirbp-mab-3v9s7-822025208454.jpg)
![Immunohistochemistry: CIRBP Antibody (3V9S7) [NBP3-15745] - Immunohistochemistry analysis of CIRBP in paraffin-embedded human thyroid tissue using CIRBP Rabbit mAb at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15745_rabbit-cirbp-mab-3v9s7-822025209899.jpg)
![Immunohistochemistry: CIRBP Antibody (3V9S7) [NBP3-15745] - Immunohistochemistry analysis of CIRBP in paraffin-embedded human pancreas tissue using CIRBP Rabbit mAb at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15745_rabbit-cirbp-mab-3v9s7-822025208483.jpg)
![Western Blot: CLIC4 Antibody [NBP2-92908] - Analysis of extracts of various cell lines, using CLIC4 antibody at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/CLIC4-Antibody-Western-Blot-NBP2-92908-img0003.jpg "Western Blot: CLIC4 Antibody [NBP2-92908] - Analysis of extracts of various cell lines, using CLIC4 antibody at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 180s.")
![Immunocytochemistry/Immunofluorescence: CLIC4 Antibody [NBP2-92908] - Analysis of A-549 cells using CLIC4 . Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/CLIC4-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-92908-img0001.jpg)
![Immunohistochemistry-Paraffin: CLIC4 Antibody [NBP2-92908] - Rat kidney using CLIC4 Rabbit pAb at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/CLIC4-Antibody-Immunohistochemistry-Paraffin-NBP2-92908-img0006.jpg)
![Immunohistochemistry-Paraffin: CLIC4 Antibody [NBP2-92908] - Human esophageal cancer using CLIC4 Rabbit pAb at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/CLIC4-Antibody-Immunohistochemistry-Paraffin-NBP2-92908-img0004.jpg)
![Immunohistochemistry-Paraffin: CLIC4 Antibody [NBP2-92908] - Mouse kidney using CLIC4 Rabbit pAb dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/CLIC4-Antibody-Immunohistochemistry-Paraffin-NBP2-92908-img0005.jpg)
![Western Blot: S100A6 Antibody (0J1Q5) [NBP3-16191] - Analysis of extracts of various cell lines, using S100A6 Rabbit mAb (NBP3-16191) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/S100A6-Antibody-0J1Q5-Western-Blot-NBP3-16191-img0004.jpg "Western Blot: S100A6 Antibody (0J1Q5) [NBP3-16191] - Analysis of extracts of various cell lines, using S100A6 Rabbit mAb (NBP3-16191) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Western Blot: S100A6 Antibody (0J1Q5) [NBP3-16191] - Analysis of extracts of various cell lines, using S100A6 Rabbit mAb (NBP3-16191) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/S100A6-Antibody-0J1Q5-Western-Blot-NBP3-16191-img0005.jpg)
![Immunohistochemistry-Paraffin: S100A6 Antibody (0J1Q5) [NBP3-16191] - Human liver using S100A6 Rabbit mAb (NBP3-16191) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/S100A6-Antibody-0J1Q5-Immunohistochemistry-Paraffin-NBP3-16191-img0002.jpg)
![Immunohistochemistry-Paraffin: S100A6 Antibody (0J1Q5) [NBP3-16191] - Rat spleen using S100A6 Rabbit mAb (NBP3-16191) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/S100A6-Antibody-0J1Q5-Immunohistochemistry-Paraffin-NBP3-16191-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: S100A6 Antibody (0J1Q5) [NBP3-16191] - Confocal imaging of C2C12 cells using S100A6 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo(R) 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16191_rabbit-s100a6-mab-0j1q5-52202511374161.jpg)
![Immunocytochemistry/ Immunofluorescence: p16INK4a/CDKN2A Antibody - BSA Free [NBP3-06986] - Validation of p16INK4a/CDKN2A In HeLa Cells. Immunofluorescent analysis of methanol-fixed HeLa cells labeling p16INK4a/CDKN2A with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/1000 dilution (red) and Hoechst staining (blue). Alpha tubulin was stained with anti-alpha tubulin antibody following by goat anti-mouse IgG secondary antibody (green). Images were captured with confocal microscopy.](http://images.novusbio.com/fullsize/nbp3-06986_rabbit-p16ink4a-cdkn2a-pab-bsa-free-271120242074659.jpg "Immunocytochemistry/ Immunofluorescence: p16INK4a/CDKN2A Antibody - BSA Free [NBP3-06986] - Validation of p16INK4a/CDKN2A In HeLa Cells. Immunofluorescent analysis of methanol-fixed HeLa cells labeling p16INK4a/CDKN2A with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/1000 dilution (red) and Hoechst staining (blue). Alpha tubulin was stained with anti-alpha tubulin antibody following by goat anti-mouse IgG secondary antibody (green). Images were captured with confocal microscopy.")
![<b>Genetic Strategies Validation. </b>Western Blot: p16INK4a/CDKN2A Antibody [NBP3-06986] - Loading: 10 ug of 293 WT cell lysates or CDKN2A KO cell lysates. Antibodies: CDKN2A, NBP3-06986 (2 ug/mL) and beta-actin (1 ug/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat](http://images.novusbio.com/fullsize/p16INK4a-CDKN2A-Antibody-Western-Blot-NBP3-06986-img0001.jpg)
![Western Blot: p16INK4a/CDKN2A Antibody [NBP3-06986] - Loading: 15 ug of lysates per lane. Antibodies: NBP3-06986 (2 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/p16INK4a-CDKN2A-Antibody-Western-Blot-NBP3-06986-img0003.jpg)
![Western Blot: p16INK4a/CDKN2A Antibody [NBP3-06986] - Loading: 15 ug of lysates per lane. Antibodies: CDKN2A (2 ug/mL), NBP3-06986 (2 ug/mL) and beta-actin (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/p16INK4a-CDKN2A-Antibody-Western-Blot-NBP3-06986-img0002.jpg)
![Immunohistochemistry: p16INK4a/CDKN2A Antibody - BSA Free [NBP3-06986] - Validation of p16INK4a/CDKN2A in Human Colon Tissue. Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-p16INK4a/CDKN2A antibody at 5 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp3-06986_rabbit-p16ink4a-cdkn2a-pab-bsa-free-2711202420115536.gif)
![Western Blot: SIAH1 Antibody [NBP2-93864] - Analysis of extracts of mouse kidney, using SIAH1 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.](http://images.novusbio.com/fullsize/SIAH1-Antibody-Western-Blot-NBP2-93864-img0005.jpg "Western Blot: SIAH1 Antibody [NBP2-93864] - Analysis of extracts of mouse kidney, using SIAH1 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.")
![Immunocytochemistry/Immunofluorescence: SIAH1 Antibody [NBP2-93864] - Immunofluorescence analysis of L929 cells using SIAH1 Rabbit pAb (NBP2-93864) at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/SIAH1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-93864-img0006.jpg)
![Immunohistochemistry-Paraffin: SIAH1 Antibody [NBP2-93864] - Rat brain using SIAH1 antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/SIAH1-Antibody-Immunohistochemistry-Paraffin-NBP2-93864-img0004.jpg)
![Immunohistochemistry-Paraffin: SIAH1 Antibody [NBP2-93864] - Human colon carcinoma using SIAH1 antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/SIAH1-Antibody-Immunohistochemistry-Paraffin-NBP2-93864-img0002.jpg)
![Immunohistochemistry-Paraffin: SIAH1 Antibody [NBP2-93864] - Mouse spleen using SIAH1 antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/SIAH1-Antibody-Immunohistochemistry-Paraffin-NBP2-93864-img0003.jpg)
![Western Blot: TSC2 Antibody (SC05-59) [NBP2-67552] - Western blot analysis of TSC2 on MCF-7 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rab](http://images.novusbio.com/fullsize/TSC2-Antibody-SC05-59-Western-Blot-NBP2-67552-img0005.jpg "Western Blot: TSC2 Antibody (SC05-59) [NBP2-67552] - Western blot analysis of TSC2 on MCF-7 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rab")
![Immunohistochemistry-Paraffin: TSC2 Antibody (SC05-59) [NBP2-67552] - Analysis of paraffin-embedded mouse placenta tissue using anti-Tuberin antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/TSC2-Antibody-SC05-59-Immunohistochemistry-Paraffin-NBP2-67552-img0002.jpg)
![Flow Cytometry: TSC2 Antibody (SC05-59) [NBP2-67552] - Flow cytometric analysis of TSC2 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes. Unlabeled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/TSC2-Antibody-SC05-59-Flow-Cytometry-NBP2-67552-img0004.jpg)
![Western Blot: TSC2 Antibody (SC05-59) [NBP2-67552] - Analysis of Tuberin on Jurkat cell lysates using anti-Tuberin antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/TSC2-Antibody-SC05-59-Western-Blot-NBP2-67552-img0003.jpg)
![Flow Cytometry: TSC2 Antibody (SC05-59) [NBP2-67552] - Analysis of Hela cells with Tuberin antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/TSC2-Antibody-SC05-59-Flow-Cytometry-NBP2-67552-img0001.jpg)
![Western Blot: PPP3CB Antibody (PSH0-21) [NBP3-32848] - Western blot analysis of PPP3CB on different lysates with Rabbit anti-PPP3CB antibody (NBP3-32848) at 1/1,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Jurkat cell lysate Lane 3: Rat brain tissue lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 59 kDa Observed band size: 59 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM for 1 hour at room temperature. The primary antibody (NBP3-32848) at 1/1,000 dilution was used in 5% NFDM at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32848_rabbit-ppp3cb-mab-psh0-21-288202412442938.png "Western Blot: PPP3CB Antibody (PSH0-21) [NBP3-32848] - Western blot analysis of PPP3CB on different lysates with Rabbit anti-PPP3CB antibody (NBP3-32848) at 1/1,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Jurkat cell lysate Lane 3: Rat brain tissue lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 59 kDa Observed band size: 59 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM for 1 hour at room temperature. The primary antibody (NBP3-32848) at 1/1,000 dilution was used in 5% NFDM at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: PPP3CB Antibody (PSH0-21) [NBP3-32848] - Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue with Rabbit anti-PPP3CB antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32848_rabbit-ppp3cb-mab-psh0-21-29820241426262.png)
![Immunocytochemistry/ Immunofluorescence: PPP3CB Antibody (PSH0-21) [NBP3-32848] - Immunocytochemistry analysis of MCF-7 cells labeling PPP3CB with Rabbit anti-PPP3CB antibody (NBP3-32848) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-PPP3CB antibody (NBP3-32848) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32848_rabbit-ppp3cb-mab-psh0-21-2920241114810.png)
![Flow Cytometry: PPP3CB Antibody (PSH0-21) [NBP3-32848] - Flow cytometric analysis of MCF-7 cells labeling PPP3CB. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32848, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32848_rabbit-ppp3cb-mab-psh0-21-29202416372124.png)