| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89498-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-81111-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-12080-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-52651-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-09006-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26278-50ul | |||
![Flow Cytometry: VE-Cadherin Antibody (048) [NBP2-89498] - Analysis of Human CD144 expression on HUVEC cells. Cells were stained with purified anti-Human CD144, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/VE-Cadherin-Antibody-048-Flow-Cytometry-NBP2-89498-img0001.jpg "Flow Cytometry: VE-Cadherin Antibody (048) [NBP2-89498] - Analysis of Human CD144 expression on HUVEC cells. Cells were stained with purified anti-Human CD144, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.")
![Immunocytochemistry/Immunofluorescence: VE-Cadherin Antibody (048) [NBP2-89498] - Staining of CDH5 in HUVEC cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-89498 antibody (dilution ratio 1:60) at 4c overnight. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp2-89498_rabbit-monoclonal-ve-cadherin-antibody-048-235202521451786.png)
![Immunohistochemistry: Lamin A + C Antibody (A6F5-R) [NBP3-32518] - Immunohistochemical analysis of paraffin-embedded human breast tissue with Mouse anti-Lamin A + C antibody (NBP3-32518) at 1/200 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32518) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32518_mouse-lamin-a-c-mab-a6f5-r-29820241426267.jpg "Immunohistochemistry: Lamin A + C Antibody (A6F5-R) [NBP3-32518] - Immunohistochemical analysis of paraffin-embedded human breast tissue with Mouse anti-Lamin A + C antibody (NBP3-32518) at 1/200 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32518) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunocytochemistry/ Immunofluorescence: Lamin A + C Antibody (A6F5-R) [NBP3-32518] - Immunocytochemistry analysis of HeLa cells labeling Lamin A + C with Mouse anti-Lamin A + C antibody (NBP3-32518) at 1/100 dilution.<br/><br/> Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Lamin A + C antibody (NBP3-32518) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32518_mouse-lamin-a-c-mab-a6f5-r-29202410484838.jpg)
![Western blot analysis of PC-12 cell lysates with NBP3-32518 at 1/2,000 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 74/65 kDa Observed band size: 74/65 kDa Exposure time: 4 minutes 22 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32518 at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat anti-Mouse IgG [HRP] at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32518_-western-blot-639204597791251056.jpg)
![Western Blot: HDAC6 Antibody (236) [NBP3-05295] - Western blotting analysis of human HDAC6 by mouse monoclonal antibody NBP3-05295 in HEK-293T cell line under reducing conditions. PVDF membrane was blocked in 5% milk, and incubated with primary antibody (1 ug/ml), followed by HRP-conjugated anti-mouse secondary antibody.](http://images.novusbio.com/fullsize/HDAC6-Antibody-236-Western-Blot-NBP3-05295-img0004.jpg "Western Blot: HDAC6 Antibody (236) [NBP3-05295] - Western blotting analysis of human HDAC6 by mouse monoclonal antibody NBP3-05295 in HEK-293T cell line under reducing conditions. PVDF membrane was blocked in 5% milk, and incubated with primary antibody (1 ug/ml), followed by HRP-conjugated anti-mouse secondary antibody.")
![Immunocytochemistry/Immunofluorescence: HDAC6 Antibody (236) [NBP3-05295] - Immunocytochemistry staining of HDAC6 in formaldehyde-fixed and Triton-permeabilized HEK-293T cells (A) and SH-SY5Y cells (B) by mouse monoclonal antibody NBP3-05295, followed by anti-mouse Alexa Fluor 488 (green), DNA indicated by DAPI (blue). In SH-SY5Y cells tubulin was stained by a rabbit polyclonal antibody, followed by anti-rabbit Alexa Fluor 647 (red, colocalization yellow).](http://images.novusbio.com/fullsize/HDAC6-Antibody-236-Immunocytochemistry-Immunofluorescence-NBP3-05295-img0001.jpg)
![ELISA: HDAC6 Antibody (236) [NBP3-05295] - ELISA analysis of human HDAC6 by mouse monoclonal antibody NBP3-05295. Plate was coated with recombinant human HDAC6, then blocked with BSA, and exposed to dilution series of anti-HDAC6 primary antibody, followed by HRP-conjugated anti-mouse secondary antibody, and colorimetric signal of processed OPD substrate was measured at 492 nm.](http://images.novusbio.com/fullsize/HDAC6-Antibody-236-ELISA-NBP3-05295-img0002.jpg)
![Immunoprecipitation: HDAC6 Antibody (236) [NBP3-05295] - Immunoprecipitation of recombinant human HDAC6 by mouse monoclonal antibodies 178 and NBP3-05295 using protein G-coated Dynabeads. Eluted proteins were processed by SDS PAAGE under reducing conditions and visualized by Coomassie Brilliant Blue.](http://images.novusbio.com/fullsize/HDAC6-Antibody-236-Immunoprecipitation-NBP3-05295-img0003.jpg)
![Flow Cytometry: Mouse Monoclonal HDAC6 Antibody (236) [NBP3-05295] - Separation of K562 cells stained using anti-human HDAC6 (236) purified antibody (concentration in sample 5.33 ug/ml, GAM APC, red-filled) from K562 cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (intracellular staining).](http://images.novusbio.com/fullsize/antibody/nbp3-05295_mouse-monoclonal-hdac6-antibody-236-flow-cytometry-1112202412584..jpg)
![Immunohistochemistry-Paraffin: CDK2 [p Tyr15] Antibody (SN72-04) [NBP2-67686] - Analysis of paraffin-embedded mouse colon tissue using anti-Phospho-Cdk2(Y15) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CDK2-p-Tyr15-Antibody-SN72-04-Immunohistochemistry-Paraffin-NBP2-67686-img0002.jpg "Immunohistochemistry-Paraffin: CDK2 [p Tyr15] Antibody (SN72-04) [NBP2-67686] - Analysis of paraffin-embedded mouse colon tissue using anti-Phospho-Cdk2(Y15) antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: CDK2 [p Tyr15] Antibody (SN72-04) [NBP2-67686] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-Phospho-Cdk2(Y15) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CDK2-p-Tyr15-Antibody-SN72-04-Immunohistochemistry-Paraffin-NBP2-67686-img0001.jpg)
![Western blot analysis of Hela cell lysates. Lane 1: Hela cells, whole cell lysate, 10ug/lane Lane 2: Hela cells treated with 2.8ug/ul lambda-PP for 30 minutes, whole cell lysates, 10ug/lane All lanes : Anti-Phospho-Cdk2(Y15) antibody (NBP2-67686) at 1/500 dilution. Anti-Cdk2 antibody at 1/500 dilution. Anti-GAPDH antibody at 1/10,000 dilution. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/200,000 dilution. Predicted band size: 34 kDa Observed band size: 34 kDa Blocking and diluting buffer: 5% BSA. Exposure time: 30 seconds](http://images.novusbio.com/fullsize/nbp2-67686_-western-blot-639204586669100018.jpg)
![Western Blot: CTCF Antibody [NBP2-59228] - Whole cell extracts (40 ug) from HeLa cells transfected with CTCF siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the antibody against CTCF diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (kDa) is shown on the left.](http://images.novusbio.com/fullsize/CTCF-Antibody-Western-Blot-NBP2-59228-img0002.jpg "Western Blot: CTCF Antibody [NBP2-59228] - Whole cell extracts (40 ug) from HeLa cells transfected with CTCF siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the antibody against CTCF diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (kDa) is shown on the left.")
![Chromatin Immunoprecipitation: CTCF Antibody [NBP2-59228] - ChIP was performed with the antibody against CTCF on sheared chromatin from 4,000,000 HeLa cells. A titration consisting of 1, 2, 5 and 10 ug of antibody per ChIP experiment was analyzed. IgG (2 ug/IP) was used as a negative IP control. Quantitative PCR was performed with optimized primers for the H19 imprinting control region, and a specific region in the GAPDH gene, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat region, used as a negative control. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/CTCF-Antibody-Chromatin-Immunoprecipitation-NBP2-59228-img0004.jpg)
![Western Blot: CTCF Antibody [NBP2-59228] - Whole cell extracts (25 ug) from HeLa cells were analysed by Western blot using the antibody against CTCF diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (kDa) is shown on the left.](http://images.novusbio.com/fullsize/CTCF-Antibody-Western-Blot-NBP2-59228-img0001.jpg)
![ELISA: CTCF Antibody [NBP2-59228] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody against CTCF. The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be >1:500,000.](http://images.novusbio.com/fullsize/CTCF-Antibody-ELISA-NBP2-59228-img0003.jpg)
![Immunofluorescence: CTCF Antibody [NBP2-59228] - HeLa cells were stained with the antibody against CTCF and with DAPI. Cells were fixed with 4% formaldehyde for 10 minutes and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the CTCF antibody (left) diluted 1:200 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa Fluor 488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/CTCF-Antibody-Immunofluorescence-NBP2-59228-img0005.jpg)
![Western Blot: N-Deacetylase/N-Sulfotransferase 1/NDST1 Antibody (1G10) [H00003340-M01] - NDST1 monoclonal antibody (M01), clone 1G10 Analysis of NDST1 expression in A-549.](http://images.novusbio.com/fullsize/N-Deacetylase-N-Sulfotransferase-1-NDST1-Antibody-1G10-Western-Blot-H00003340-M01-img0007.jpg "Western Blot: N-Deacetylase/N-Sulfotransferase 1/NDST1 Antibody (1G10) [H00003340-M01] - NDST1 monoclonal antibody (M01), clone 1G10 Analysis of NDST1 expression in A-549.")
![Immunohistochemistry-Paraffin: N-Deacetylase/N-Sulfotransferase 1/NDST1 Antibody (1G10) [H00003340-M01] - Analysis of monoclonal antibody to NDST1 on formalin-fixed paraffin-embedded human small Intestine. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/N-Deacetylase-N-Sulfotransferase-1-NDST1-Antibody-1G10-Immunohistochemistry-Paraffin-H00003340-M01-img0005.jpg)
![Western Blot: N-Deacetylase/N-Sulfotransferase 1/NDST1 Antibody (1G10) [H00003340-M01] - Western Blot analysis of NDST1 expression in A-549 ( Cat # L025V1 ).](http://images.novusbio.com/fullsize/N-Deacetylase-N-Sulfotransferase-1-NDST1-Antibody-1G10-Western-Blot-H00003340-M01-img0001.jpg)
![Sandwich ELISA: N-Deacetylase/N-Sulfotransferase 1/NDST1 Antibody (1G10) [H00003340-M01] - Detection limit for recombinant GST tagged NDST1 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/N-Deacetylase-N-Sulfotransferase-1-NDST1-Antibody-1G10-Sandwich-ELISA-H00003340-M01-img0006.jpg)
![Flow Cytometry: Fibrin beta-chain Antibody (8E5) - Chimeric [NBP2-81111] - Flow-cytometry using the Fibrin beta-chain Antibody [NBP2-81111]. HepG2 cells were fixed using 2% PFA, permeabilised using 0.5% Triton and stained with anti-Fluorescein IgG antibody (isotype control, black line) or the rabbit IgG-chimeric version of 8E5 (NBP2-81111, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/Fibrinopeptide-B-Antibody-8E5-Chimeric-Flow-Cytometry-NBP2-81111-img0001.jpg "Flow Cytometry: Fibrin beta-chain Antibody (8E5) - Chimeric [NBP2-81111] - Flow-cytometry using the Fibrin beta-chain Antibody [NBP2-81111]. HepG2 cells were fixed using 2% PFA, permeabilised using 0.5% Triton and stained with anti-Fluorescein IgG antibody (isotype control, black line) or the rabbit IgG-chimeric version of 8E5 (NBP2-81111, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.")
![Immunocytochemistry/Immunofluorescence: CD81 Antibody (1.3.3.22) - Chimeric [NBP3-12080] - Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of 1.3.3.22 (NBP3-12080) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-12080, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/CD81-Antibody-1-3-3-22-Chimeric-Immunocytochemistry-Immunofluorescence-NBP3-12080-img0001.jpg "Immunocytochemistry/Immunofluorescence: CD81 Antibody (1.3.3.22) - Chimeric [NBP3-12080] - Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of 1.3.3.22 (NBP3-12080) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-12080, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.")
![Flow Cytometry: CD81 Antibody (1.3.3.22) - Chimeric - Azide and BSA Free [NBP3-12080] - Paraformaldehyde fixed Daudi cells were stained with anti-unknown specificity antibody (isotype control, black line) or NBP3-12080 (blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/nbp3-12080_rabbit-monoclonal-cd81-antibody-1-3-3-22-chimeric-74202522141241.png)
![Immunocytochemistry /Immunofluorescence: EGFR Antibody (528) - Chimeric - Azide and BSA Free [NBP2-52651] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton and stained with NBP2-52651 at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-52651, DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp2-52651_rabbit-monoclonal-egfr-antibody-528-chimeric-74202522141227.png "Immunocytochemistry /Immunofluorescence: EGFR Antibody (528) - Chimeric - Azide and BSA Free [NBP2-52651] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton and stained with NBP2-52651 at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-52651, DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor 488 secondary antibody.")
![Western Blot: alpha Tubulin Antibody (F2C) - Chimeric [NBP3-09006] - HeLa (A), HEK293 (B), and HepG2 (C) cell lysate samples (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of F2C (NBP3-09006) at 0.01 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for alpha Tubulin is ~55kDa. NBP3-09006 successfully detected human alpha Tubulin in HeLa, HEK293, and HepG2 cell lysate samples.](http://images.novusbio.com/fullsize/alpha-Tubulin-Antibody-F2C-Chimeric-Western-Blot-NBP3-09006-img0002.jpg "Western Blot: alpha Tubulin Antibody (F2C) - Chimeric [NBP3-09006] - HeLa (A), HEK293 (B), and HepG2 (C) cell lysate samples (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of F2C (NBP3-09006) at 0.01 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for alpha Tubulin is ~55kDa. NBP3-09006 successfully detected human alpha Tubulin in HeLa, HEK293, and HepG2 cell lysate samples.")
![Immunocytochemistry/Immunofluorescence: alpha Tubulin Antibody (F2C) - Chimeric [NBP3-09006] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of F2C (NBP3-09006) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (1 ug/ml), showing microtubule staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-09006, DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/alpha-Tubulin-Antibody-F2C-Chimeric-Immunocytochemistry-Immunofluorescence-NBP3-09006-img0004.jpg)
![Immunohistochemistry-Paraffin: alpha Tubulin Antibody (F2C) - Chimeric [NBP3-09006] - Anti-Alpha Tubulin staining of paraffin embedded human cerebral cortex tissue using the rabbit-chimeric version of F2C (NBP3-09006). Antigen retreival was acheived by microwaving in citrate buffer (pH6), followed by blocking with protein block serum-free buffer. Primary antibody incubation with NBP3-09006 was carried out at 4 ug/ml for 30 minutes. Samples were then incubated with an anti-rabbit IgG HRP secondary antibody for 20 mins followed by DAB (3,3'-diaminobenzidine), and counter-staining with haemotoxylin. Staining of axons and a few neuronal cell bodies may be observed. Recommended concentration, 2-4 ug/ml.](http://images.novusbio.com/fullsize/alpha-Tubulin-Antibody-F2C-Chimeric-Immunohistochemistry-Paraffin-NBP3-09006-img0001.jpg)
![Flow Cytometry: alpha Tubulin Antibody (F2C) - Chimeric [NBP3-09006] - HepG2 (A), HeLa (B) and Hek293 (C) cells were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of F2C (NBP3-09006, blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using anti-rabbit IgG JK (FITC-conjugate) antibody (129936) at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/alpha-Tubulin-Antibody-F2C-Chimeric-Flow-Cytometry-NBP3-09006-img0003.jpg)
![Western Blot: c-Abl Antibody (M209) [NBP3-23312] - Western blot analysis of K-562 cells treated with pervanadate (1 mM) for 30 minutes (lanes 1, 3, & 5). Some lanes were treated with alkaline phosphatase to remove phosphorylation on c-Abl (lanes 2, 4, & 6), then the blots were probed with anti-c-Abl (lanes 1 & 2), anti-c-Abl (Tyr-412) (NBP3-23318; lanes 3 & 4), or anti-c-Abl (Tyr-245) (NBP3-23313; lanes 5 & 6).](http://images.novusbio.com/fullsize/nbp3-23312_mouse-c-abl-mab-m209-9820231138390.jpg "<b>Biological Strategies Validation. </b>Western Blot: c-Abl Antibody (M209) [NBP3-23312] - Western blot analysis of K-562 cells treated with pervanadate (1 mM) for 30 minutes (lanes 1, 3, & 5). Some lanes were treated with alkaline phosphatase to remove phosphorylation on c-Abl (lanes 2, 4, & 6), then the blots were probed with anti-c-Abl (lanes 1 & 2), anti-c-Abl (Tyr-412) (NBP3-23318; lanes 3 & 4), or anti-c-Abl (Tyr-245) (NBP3-23313; lanes 5 & 6).")
![<b>Biological Strategies Validation. </b>Western Blot: c-Abl Antibody (M209) [NBP3-23312] - Western blot analysis of K-562 cells treated with pervanadate (1 mM) for 30 minutes (lanes 1, 3, & 5). Some lanes were treated with alkaline phosphatase to remove phosphorylation on c-Abl (lanes 2, 4, & 6), then the blots were probed with anti-c-Abl (lanes 1 & 2), anti-c-Abl (Tyr-412) (NBP3-23318; lanes 3 & 4), or anti-c-Abl (Tyr-245) (NBP3-23313; lanes 5 & 6).](http://images.novusbio.com/fullsize/nbp3-23312_mouse-c-abl-mab-m209-19920231915412.jpg)
![Immunoprecipitation: Topoisomerase I Antibody (6D8) [NBP3-26278] - Immunoprecipitating Topoisomerase I in K562 whole cell lysate. Lane 1: Rabbit control IgG instead of NBP3-26278 in K562 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: NBP3-26278 (2ug) + K562 whole cell lysate (500ug). Lane 3: K562 whole cell lysate (10ug).](http://images.novusbio.com/fullsize/nbp3-26278_rabbit-topoisomerase-i-mab-6d8-2622024132372.jpg "Immunoprecipitation: Topoisomerase I Antibody (6D8) [NBP3-26278] - Immunoprecipitating Topoisomerase I in K562 whole cell lysate. Lane 1: Rabbit control IgG instead of NBP3-26278 in K562 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: NBP3-26278 (2ug) + K562 whole cell lysate (500ug). Lane 3: K562 whole cell lysate (10ug).")
![Immunohistochemistry: Topoisomerase I Antibody (6D8) [NBP3-26278] - Image of Topoisomerase I Antibody (6D8) diluted at 1:100 and staining in paraffin-embedded human small intestine tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26278_rabbit-topoisomerase-i-mab-6d8-26220241447153.jpg)
![Immunohistochemistry: Topoisomerase I Antibody (6D8) [NBP3-26278] - Image of Topoisomerase I Antibody (6D8) diluted at 1:100 and staining in paraffin-embedded human small intestine tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26278_rabbit-topoisomerase-i-mab-6d8-2722024856319.jpg)
![Western Blot: Topoisomerase I Antibody (6D8) [NBP3-26278] - Positive Western Blot detected in: Hela whole cell lysate, MCF-7 whole cell lysate, K562 whole cell lysate, HL60 whole cell lysate, PC-3 whole cell lysate. All lanes: Topoisomerase I Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 91 kDa Observed band size: 91 kDa](http://images.novusbio.com/fullsize/nbp3-26278_rabbit-topoisomerase-i-mab-6d8-28220241144269.jpg)
![Flow Cytometry: Topoisomerase I Antibody (6D8) [NBP3-26278] - Overlay histogram showing HepG2 cells stained with Topoisomerase I Antibody (6D8) (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 1 h at 4C. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4C. Control antibody (green line) was Rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26278_rabbit-topoisomerase-i-mab-6d8-282202414312734.jpg)