| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35288-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-2648-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26692-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21697-50ul | |||
![Western Blot: CDC25C Antibody (6R0I6) [NBP3-15437] - Western blot analysis of extracts of various cell lines, using CDC25C antibody (NBP3-15437) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/CDC25C-Antibody-6R0I6-Western-Blot-NBP3-15437-img0002.jpg "Western Blot: CDC25C Antibody (6R0I6) [NBP3-15437] - Western blot analysis of extracts of various cell lines, using CDC25C antibody (NBP3-15437) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.")
![Immunocytochemistry/ Immunofluorescence: CDC25C Antibody (6R0I6) [NBP3-15437] - Immunofluorescence analysis of HeLa cells using CDC25C Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-15437_rabbit-cdc25c-mab-6r0i6-72202512435382.jpg)
![Western Blot: Cdk7 Antibody [NBP3-35288] - Western blot analysis of various lysates using [KO Validated] Cdk7 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 15s.](http://images.novusbio.com/fullsize/nbp3-35288_rabbit-cdk7-pab-10122024120667.jpg "Western Blot: Cdk7 Antibody [NBP3-35288] - Western blot analysis of various lysates using [KO Validated] Cdk7 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 15s.")
![<b>Genetic Strategies Validation. </b>Western Blot: Cdk7 Antibody [NBP3-35288] - Western Blot analysis of lysates from wild type (WT) and Cdk7 knockout (KO) 293T cells, using [KO Validated] Cdk7 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.](http://images.novusbio.com/fullsize/nbp3-35288_rabbit-cdk7-pab-31120251744483.jpg%3Ft=1709093634)
![Western Blot: ZWINT Antibody [NB100-2364] - Detection of Human ZWINT-1 on HeLa whole cell lysate using NB100-2364. ZWINT-1 was also immunoprecipitated by rabbit anti-ZWINT-1 antibody NB100-2363.](http://images.novusbio.com/fullsize/ZWINT-Antibody-Western-Blot-NB100-2364-img0004.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: ZWINT Antibody [NB100-2364] - Detection of Human ZWINT-1 on HeLa whole cell lysate using NB100-2364. ZWINT-1 was also immunoprecipitated by rabbit anti-ZWINT-1 antibody NB100-2363.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunocytochemistry/Immunofluorescence: ZWINT Antibody [NB100-2364] - NBF-fixed asynchronous HeLa cells. Affinity purified rabbit anti-ZWINT-1 used at a dilution of 1:250. Detection: Anti-rabbit IgG-FITC conjugated used at a dilution of 1:100.](http://images.novusbio.com/fullsize/ZWINT-Antibody-Immunocytochemistry-Immunofluorescence-NB100-2364-img0002.jpg)
![Western Blot: E2F8 Antibody [NBP1-52650] - Detection of human E2F8 by western blot. Samples: Whole cell lysate (10 ug) from HEK293T and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-E2F8 antibody NBP1-52650 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/E2F8-Antibody-Western-Blot-NBP1-52650-img0006.jpg "Western Blot: E2F8 Antibody [NBP1-52650] - Detection of human E2F8 by western blot. Samples: Whole cell lysate (10 ug) from HEK293T and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-E2F8 antibody NBP1-52650 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Immunoprecipitation: E2F8 Antibody [NBP1-52650] - Detection of human E2F8 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-E2F8 antibody NBP1-52650(lot NBP1-52650-3) used for IP at 6 ug per reaction. E2F8 was also immunoprecipitated by a previous lot of this antibody (lot NBP1-52650-2). For blotting immunoprecipitated E2F8, NBP1-52650 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/E2F8-Antibody-Immunoprecipitation-NBP1-52650-img0007.jpg)
![Immunohistochemistry: Cyclin E1 Antibody (3A5) [NBP3-26692] - Image of Cyclin E1 Antibody (3A5) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26692_rabbit-cyclin-e1-mab-3a5-262202414471591.jpg "Immunohistochemistry: Cyclin E1 Antibody (3A5) [NBP3-26692] - Image of Cyclin E1 Antibody (3A5) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Immunohistochemistry: Cyclin E1 Antibody (3A5) [NBP3-26692] - Image of Cyclin E1 Antibody (3A5) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26692_rabbit-cyclin-e1-mab-3a5-262202415253332.jpg)
![Immunocytochemistry/Immunofluorescence: Cyclin E1 Antibody (3A5) [NBP3-26692] - Staining of Hela Cells with Cyclin E1 Antibody (3A5) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26692_rabbit-cyclin-e1-mab-3a5-282202412493621.jpg)
![Immunohistochemistry: ANAPC2 Antibody - BSA Free [NBP3-48130] - Immunohistochemistry of paraffin-embedded human gliomas using (ANAPC2 antibody) at dilution of 1:100](http://images.novusbio.com/fullsize/nbp3-48130_rabbit-anapc2-pab-bsa-free-2842025113606.jpg "Immunohistochemistry: ANAPC2 Antibody - BSA Free [NBP3-48130] - Immunohistochemistry of paraffin-embedded human gliomas using (ANAPC2 antibody) at dilution of 1:100")
![Western Blot: ANAPC2 Antibody - BSA Free [NBP3-48130] - MCF7 cells were subjected to SDS PAGE followed by western blot with (ANAPC2 antibody) at dilution of 1:500](http://images.novusbio.com/fullsize/nbp3-48130_rabbit-anapc2-pab-bsa-free-284202511364639.jpg)
![Western Blot: CDC25C Antibody (SR2051) [NBP3-21697] - Western blot analysis using NBP3-21697 in K562 cell lysate.](http://images.novusbio.com/fullsize/nbp3-21697_rabbit-cdc25c-mab-sr2051-57202317161892.jpg "Western Blot: CDC25C Antibody (SR2051) [NBP3-21697] - Western blot analysis using NBP3-21697 in K562 cell lysate.")
![Western Blot: Cyclin B1 Antibody (RM281) [NBP2-61517] - Western Blot of Jurkat cell lysates using NBP2-61517 at a 1:1000 dilution](http://images.novusbio.com/fullsize/Cyclin-B1-Antibody-RM281-Western-Blot-NBP2-61517-img0004.jpg "Western Blot: Cyclin B1 Antibody (RM281) [NBP2-61517] - Western Blot of Jurkat cell lysates using NBP2-61517 at a 1:1000 dilution")
![Immunohistochemistry-Paraffin: Cyclin B1 Antibody (RM281) [NBP2-61517] - Immunohistochemical staining of formalin fixed and paraffin embedded human tonsil tissue sections using NBP2-61517 at a 1:1000 dilution.](http://images.novusbio.com/fullsize/Cyclin-B1-Antibody-RM281-Immunohistochemistry-Paraffin-NBP2-61517-img0005.jpg)
![Immunohistochemistry-Paraffin: Cyclin B1 Antibody (RM281) [NBP2-61517] - Immunohistochemical staining of formalin fixed and paraffin embedded Human breast cancer tissue sections using NBP2-61517 at a 1:2000 dilution](http://images.novusbio.com/fullsize/nbp2-61517_rabbit-monoclonal-cyclin-b1-antibody-rm281-immunohistochemistry-paraffin-189202523374013.png)
![Western Blot: Aurora B Antibody (RM278) [NBP2-61493] - Western Blot of HeLa cells nontreated (-) or treated (+) with Nocodazole (NOC), using NBP2-61493 at a 1:1000 dilution](http://images.novusbio.com/fullsize/Aurora-B-Antibody-RM278-Western-Blot-NBP2-61493-img0004.jpg "Western Blot: Aurora B Antibody (RM278) [NBP2-61493] - Western Blot of HeLa cells nontreated (-) or treated (+) with Nocodazole (NOC), using NBP2-61493 at a 1:1000 dilution")
![Immunohistochemistry-Paraffin: Aurora B Antibody (RM278) [NBP2-61493] - Immunohistochemical staining of formalin fixed and paraffin embedded Human tonsil tissue sections using NBP2-61493 at a 1:500 dilution](http://images.novusbio.com/fullsize/nbp2-61493_rabbit-monoclonal-aurora-b-antibody-rm278-immunohistochemistry-paraffin-189202523403518.png)
![Western Blot: ZWINT Antibody (6D3) [H00011130-M01] - ZWINT monoclonal antibody (M01), clone 6D3 Analysis of ZWINT expression in Hela S3 NE.](http://images.novusbio.com/fullsize/ZWINT-Antibody-6D3-Western-Blot-H00011130-M01-img0007.jpg "Western Blot: ZWINT Antibody (6D3) [H00011130-M01] - ZWINT monoclonal antibody (M01), clone 6D3 Analysis of ZWINT expression in Hela S3 NE.")
![Immunocytochemistry/Immunofluorescence: ZWINT Antibody (6D3) [H00011130-M01] - Analysis of monoclonal antibody to ZWINT on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/ZWINT-Antibody-6D3-Immunocytochemistry-Immunofluorescence-H00011130-M01-img0005.jpg)
![Sandwich ELISA: ZWINT Antibody (6D3) [H00011130-M01] - Detection limit for recombinant GST tagged ZWINT is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/ZWINT-Antibody-6D3-Sandwich-ELISA-H00011130-M01-img0006.jpg)
![Western Blot: NDC80 Antibody (1A10) [H00010403-M01] - KNTC2 monoclonal antibody (M01), clone 1A10 Analysis of KNTC2 expression in Hela S3 NE.](http://images.novusbio.com/fullsize/NDC80-Antibody-1A10-Western-Blot-H00010403-M01-img0004.jpg "Western Blot: NDC80 Antibody (1A10) [H00010403-M01] - KNTC2 monoclonal antibody (M01), clone 1A10 Analysis of KNTC2 expression in Hela S3 NE.")
![Sandwich ELISA: NDC80 Antibody (1A10) [H00010403-M01] - Detection limit for recombinant GST tagged KNTC2 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/NDC80-Antibody-1A10-Sandwich-ELISA-H00010403-M01-img0003.jpg)