| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-03559-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38030-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61734-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21529-50ul | |||
![Western Blot: CSN1 Antibody [NB100-1237] - Human Testis lysate (RIPA buffer, 30 ug total protein). Antibody at 0.5 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/CSN1-Antibody-Western-Blot-NB100-1237-img0002.jpg "Western Blot: CSN1 Antibody [NB100-1237] - Human Testis lysate (RIPA buffer, 30 ug total protein). Antibody at 0.5 ug/mL. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: CSN1 Antibody [NB100-1237] - Staining of paraffin embedded Human Tonsil. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/CSN1-Antibody-Immunohistochemistry-Paraffin-NB100-1237-img0005.jpg)
![Immunohistochemistry-Paraffin: CSN1 Antibody [NB100-1237] - Staining of paraffin embedded Human Prostate. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/CSN1-Antibody-Immunohistochemistry-Paraffin-NB100-1237-img0003.jpg)
![Immunohistochemistry-Paraffin: CSN1 Antibody [NB100-1237] - Staining of paraffin embedded Human Testis. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/CSN1-Antibody-Immunohistochemistry-Paraffin-NB100-1237-img0004.jpg)
![Western Blot: MLL5 Antibody [NBP3-03559] - Western blot analysis of extracts of C2C12 cells, using (NBP3-03559) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/MLL5 Antibody-Western Blot-NBP3-03559-img0005.jpg "Western Blot: MLL5 Antibody [NBP3-03559] - Western blot analysis of extracts of C2C12 cells, using (NBP3-03559) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.")
![Immunohistochemistry: MLL5 Antibody [NBP3-03559] - Analysis of mouse brain using MLL5 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/MLL5-Antibody-Immunohistochemistry-NBP3-03559-img0001.jpg)
![Immunohistochemistry: MLL5 Antibody - Azide and BSA Free [MLL5] - Immunohistochemistry analysis of paraffin-embedded Rat lung using MLL5 Rabbit pAb at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-03559_rabbit-polyclonal-mll5-antibody-52202516321453.jpg)
![Immunohistochemistry: MLL5 Antibody - Azide and BSA Free [MLL5] - Immunohistochemistry analysis of paraffin-embedded Rat spleen using MLL5 Rabbit pAb at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-03559_rabbit-polyclonal-mll5-antibody-5220251639636.jpg)
![Immunocytochemistry/ Immunofluorescence: PAC1R Antibody [NBP3-38030] - Immunofluorescence analysis of SH-SY5Y cells using PAC1R Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38030_rabbit-pac1r-pab-1012202411531046.jpg "Immunocytochemistry/ Immunofluorescence: PAC1R Antibody [NBP3-38030] - Immunofluorescence analysis of SH-SY5Y cells using PAC1R Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Western Blot: PAC1R Antibody [NBP3-38030] - Western blot analysis of lysates from Mouse heart , using PAC1R Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.](http://images.novusbio.com/fullsize/nbp3-38030_rabbit-pac1r-pab-10122024120621.jpg)
![Western Blot: PAC1R Antibody [NBP3-38030] - Western blot analysis of lysates from U-87MG cells, using PAC1R Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-38030_rabbit-pac1r-pab-1012202411535820.jpg)
![Immunocytochemistry/ Immunofluorescence: PAC1R Antibody [NBP3-38030] - Immunofluorescence analysis of NIH/3T3 cells using PAC1R Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38030_rabbit-pac1r-pab-10122024120660.jpg)
![Immunocytochemistry/ Immunofluorescence: PAC1R Antibody [NBP3-38030] - Immunofluorescence analysis of C6 cells using PAC1R Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38030_rabbit-pac1r-pab-1012202411535868.jpg)
![Western Blot: Aromatase Antibody (5C5N7) [NBP3-15438] - Western blot analysis of extracts of U-87MG cells, using Aromatase antibody (NBP3-15438) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/Aromatase-Antibody-5C5N7-Western-Blot-NBP3-15438-img0003.jpg "Western Blot: Aromatase Antibody (5C5N7) [NBP3-15438] - Western blot analysis of extracts of U-87MG cells, using Aromatase antibody (NBP3-15438) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.")
![Immunohistochemistry: Aromatase Antibody (5C5N7) [NBP3-15438] - Immunofluorescence analysis of human placenta using Aromatase Rabbit mAb (NBP3-15438) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Aromatase-Antibody-5C5N7-Immunohistochemistry-NBP3-15438-img0002.jpg)
![Immunohistochemistry-Paraffin: Aromatase Antibody (5C5N7) [NBP3-15438] - Human colon using Aromatase Rabbit mAb (NBP3-15438) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Aromatase-Antibody-5C5N7-Immunohistochemistry-Paraffin-NBP3-15438-img0001.jpg)
![Immunohistochemistry: Aromatase Antibody (5C5N7) [Aromatase] - Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using Aromatase Rabbit mAb at a dilution of 1:4000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15438_rabbit-aromatase-mab-5c5n7-52202517273588.jpg)
![Immunohistochemistry: Aromatase Antibody (5C5N7) [Aromatase] - Immunohistochemistry analysis of paraffin-embedded Human placenta tissue using Aromatase Rabbit mAb at a dilution of 1:4000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15438_rabbit-aromatase-mab-5c5n7-5220251723570.jpg)
![Western Blot: Cyclin B2 Antibody (1L9B9) [NBP3-16663] - Western blot analysis of extracts of Rat testis cells, using Cyclin B2 antibody (NBP3-16663) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/Cyclin-B2-Antibody-1L9B9-Western-Blot-NBP3-16663-img0003.jpg "Western Blot: Cyclin B2 Antibody (1L9B9) [NBP3-16663] - Western blot analysis of extracts of Rat testis cells, using Cyclin B2 antibody (NBP3-16663) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Immunocytochemistry/Immunofluorescence: Cyclin B2 Antibody (1L9B9) [NBP3-16663] - Immunofluorescence analysis of NIH-3T3 cells using Cyclin B2 Rabbit mAb (NBP3-16663) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Cyclin-B2-Antibody-1L9B9-Immunocytochemistry-Immunofluorescence-NBP3-16663-img0002.jpg)
![Immunohistochemistry-Paraffin: Cyclin B2 Antibody (1L9B9) [NBP3-16663] - Immunohistochemistry of paraffin-embedded human liver cancer using Cyclin B2 Rabbit mAb (NBP3-16663) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Cyclin-B2-Antibody-1L9B9-Immunohistochemistry-Paraffin-NBP3-16663-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: Cyclin B2 Antibody (1L9B9) [NBP3-16663] - Confocal imaging of NIH/3T3 cells using Cyclin B2 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo(R) 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16663_rabbit-cyclin-b2-mab-1l9b9-52202511392244.jpg)
![Western Blot: CIRBP Antibody (6E12B3) [NBP2-61734] - Analysis using CIRBP mAb against HEK293 (1) and CIRBP (AA: 1-90)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/CIRBP-Antibody-6E12B3-Western-Blot-NBP2-61734-img0005.jpg "Western Blot: CIRBP Antibody (6E12B3) [NBP2-61734] - Analysis using CIRBP mAb against HEK293 (1) and CIRBP (AA: 1-90)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunohistochemistry: CIRBP Antibody (6E12B3) [NBP2-61734] - Analysis of paraffin-embedded rectum cancer tissues using CIRBP mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/CIRBP-Antibody-6E12B3-Immunohistochemistry-NBP2-61734-img0003.jpg)
![Western Blot: CIRBP Antibody (6E12B3) [NBP2-61734] - Analysis using CIRBP mAb against human CIRBP (AA: 1-90) recombinant protein. (Expected MW is 35.9 kDa)](http://images.novusbio.com/fullsize/CIRBP-Antibody-6E12B3-Western-Blot-NBP2-61734-img0004.jpg)
![Western Blot: CIRBP Antibody (6E12B3) [NBP2-61734] - Analysis using CIRBP mouse mAb against SW480 (1), PC-3 (2), A431 (3), and U251 (4) cell lysate.](http://images.novusbio.com/fullsize/CIRBP-Antibody-6E12B3-Western-Blot-NBP2-61734-img0006.jpg)
![Immunohistochemistry: CIRBP Antibody (6E12B3) [NBP2-61734] - Analysis of paraffin-embedded bladder cancer tissues using CIRBP mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/CIRBP-Antibody-6E12B3-Immunohistochemistry-NBP2-61734-img0002.jpg)
![ELISA: CIRBP Antibody (6E12B3) [NBP2-61734] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/CIRBP-Antibody-6E12B3-ELISA-NBP2-61734-img0001.jpg)
![Western Blot: KIFC1 Antibody (JE00-46) [NBP3-32504] - Western blot analysis of KIFC1 on different lysates with Rabbit anti-KIFC1 antibody (NBP3-32504) at 1/1,000 dilution. Lane 1: Jurkat cell lysate Lane 2: HT-29 cell lysate Lane 3: HeLa cell lysate Lane 4: MCF7 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 74 kDa Observed band size: 70 kDa Exposure time: 43 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32504) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32504_rabbit-kifc1-mab-je00-46-278202415242619.jpg "Western Blot: KIFC1 Antibody (JE00-46) [NBP3-32504] - Western blot analysis of KIFC1 on different lysates with Rabbit anti-KIFC1 antibody (NBP3-32504) at 1/1,000 dilution. Lane 1: Jurkat cell lysate Lane 2: HT-29 cell lysate Lane 3: HeLa cell lysate Lane 4: MCF7 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 74 kDa Observed band size: 70 kDa Exposure time: 43 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32504) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: KIFC1 Antibody (JE00-46) [NBP3-32504] - Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-KIFC1 antibody (NBP3-32504) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32504) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32504_rabbit-kifc1-mab-je00-46-298202414262691.jpg)
![Immunocytochemistry/ Immunofluorescence: KIFC1 Antibody (JE00-46) [NBP3-32504] - Immunocytochemistry analysis of Jurkat cells labeling KIFC1 with Rabbit anti-KIFC1 antibody (NBP3-32504) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-KIFC1 antibody (NBP3-32504) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32504_rabbit-kifc1-mab-je00-46-29202410334073.jpg)
![Flow Cytometry: KIFC1 Antibody (JE00-46) [NBP3-32504] - Flow cytometric analysis of Jurkat cells labeling KIFC1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32504, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32504_rabbit-kifc1-mab-je00-46-29202416453263.jpg)
![Western Blot: CTCF Antibody [NBP2-59228] - Whole cell extracts (40 ug) from HeLa cells transfected with CTCF siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the antibody against CTCF diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (kDa) is shown on the left.](http://images.novusbio.com/fullsize/CTCF-Antibody-Western-Blot-NBP2-59228-img0002.jpg "Western Blot: CTCF Antibody [NBP2-59228] - Whole cell extracts (40 ug) from HeLa cells transfected with CTCF siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the antibody against CTCF diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (kDa) is shown on the left.")
![Chromatin Immunoprecipitation: CTCF Antibody [NBP2-59228] - ChIP was performed with the antibody against CTCF on sheared chromatin from 4,000,000 HeLa cells. A titration consisting of 1, 2, 5 and 10 ug of antibody per ChIP experiment was analyzed. IgG (2 ug/IP) was used as a negative IP control. Quantitative PCR was performed with optimized primers for the H19 imprinting control region, and a specific region in the GAPDH gene, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat region, used as a negative control. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/CTCF-Antibody-Chromatin-Immunoprecipitation-NBP2-59228-img0004.jpg)
![Western Blot: CTCF Antibody [NBP2-59228] - Whole cell extracts (25 ug) from HeLa cells were analysed by Western blot using the antibody against CTCF diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (kDa) is shown on the left.](http://images.novusbio.com/fullsize/CTCF-Antibody-Western-Blot-NBP2-59228-img0001.jpg)
![ELISA: CTCF Antibody [NBP2-59228] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody against CTCF. The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be >1:500,000.](http://images.novusbio.com/fullsize/CTCF-Antibody-ELISA-NBP2-59228-img0003.jpg)
![Immunofluorescence: CTCF Antibody [NBP2-59228] - HeLa cells were stained with the antibody against CTCF and with DAPI. Cells were fixed with 4% formaldehyde for 10 minutes and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the CTCF antibody (left) diluted 1:200 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa Fluor 488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/CTCF-Antibody-Immunofluorescence-NBP2-59228-img0005.jpg)
![Western Blot: Cyclin B2 Antibody [NBP1-33031] - Sample (30 ug of whole cell lysate) A: H1299 10% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Cyclin-B2-Antibody-Western-Blot-NBP1-33031-img0004.jpg "Western Blot: Cyclin B2 Antibody [NBP1-33031] - Sample (30 ug of whole cell lysate) A: H1299 10% SDS PAGE; antibody diluted at 1:1000.")
![Immunohistochemistry-Paraffin: Cyclin B2 Antibody [NBP1-33031] - Cyclin B2 Antibody detects Cyclin B2 protein at cytoplasm in rat testis by immunohistochemical analysis. Sample: Paraffin-embedded rat testis. Cyclin B2 Antibody diluted at 1:500.](http://images.novusbio.com/fullsize/Cyclin-B2-Antibody-Immunohistochemistry-Paraffin-NBP1-33031-img0005.jpg)
![Immunohistochemistry-Paraffin: Cyclin B2 Antibody [NBP1-33031] - Paraffin-embedded H1299 xenograft; antibody dilution 1:500.](http://images.novusbio.com/fullsize/Cyclin-B2-Antibody-Immunohistochemistry-Paraffin-NBP1-33031-img0003.jpg)
![Cyclin B2 antibody [N1C3-2] detects CCNB2 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: CCNB2 protein stained by Cyclin B2 antibody [N1C3-2] (NBP1-33031) diluted at 1:500.Blue: Hoechst 33343 staining.](http://images.novusbio.com/fullsize/nbp1-33031_rabbit-polyclonal-cyclin-b2-antibody-immunocytochemistry-immunofluorescence-2611202518393122.jpg)
![Western Blot: LIS1 Antibody [NB200-326] - Detection of Human, Mouse and Rat LIS-1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, K562, mouse TCMK-1, and rat C6 cells. Antibodies: Affinity purified rabbit anti-LIS-1 antibody NB200-326 used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/LIS1-Antibody-Western-Blot-NB200-326-img0004.jpg "<b>Biological Strategies Validation. </b>Western Blot: LIS1 Antibody [NB200-326] - Detection of Human, Mouse and Rat LIS-1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, K562, mouse TCMK-1, and rat C6 cells. Antibodies: Affinity purified rabbit anti-LIS-1 antibody NB200-326 used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![<b>Biological Strategies Validation. </b>Western Blot: LIS1 Antibody [NB200-326] - Whole cell lysate (50 ug) from TF-1 (erythroleukemia) cells. Antibody used at the indicated concentrations.](http://images.novusbio.com/fullsize/LIS1-Antibody-Western-Blot-NB200-326-img0002.jpg)
![Western Blot: JNK1/2/3 [p Thr 183] Antibody (SR1788) [NBP3-21529] - Western blot analysis using NBP3-21529 in NIH/3T3 cell lysate treated with Anisomycin.](http://images.novusbio.com/fullsize/nbp3-21529_rabbit-jnk1-2-3-p-thr-183-mab-sr1788-572023156479.jpg "Western Blot: JNK1/2/3 [p Thr 183] Antibody (SR1788) [NBP3-21529] - Western blot analysis using NBP3-21529 in NIH/3T3 cell lysate treated with Anisomycin.")
![Immunohistochemistry: JNK1/2/3 [p Thr 183] Antibody (SR1788) [NBP3-21529] - Immunohistochemical analysis of paraffin-embedded human breast, using NBP3-21529.](http://images.novusbio.com/fullsize/nbp3-21529_rabbit-jnk1-2-3-p-thr-183-mab-sr1788-5720231558484.jpg)
![Immunocytochemistry/Immunofluorescence: JNK1/2/3 [p Thr 183] Antibody (SR1788) [NBP3-21529] - Immunofluorescent analysis of NIH/3T3 cells treated with Anisomycin, using NBP3-21529.](http://images.novusbio.com/fullsize/nbp3-21529_rabbit-jnk1-2-3-p-thr-183-mab-sr1788-6720231051264.jpg)
![Western Blot: LIS1 Antibody (5A5) [H00005048-M03] - Analysis of PAFAH1B1 expression in transfected 293T cell line by PAFAH1B1 monoclonal antibody (M03), clone 5A5. Lane 1: PAFAH1B1 transfected lysatE (46.6 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/LIS1-Antibody-5A5-Western-Blot-H00005048-M03-img0004.jpg "Western Blot: LIS1 Antibody (5A5) [H00005048-M03] - Analysis of PAFAH1B1 expression in transfected 293T cell line by PAFAH1B1 monoclonal antibody (M03), clone 5A5. Lane 1: PAFAH1B1 transfected lysatE (46.6 KDa). Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: LIS1 Antibody (5A5) [H00005048-M03] - Analysis of monoclonal antibody to PAFAH1B1 on HeLa cell. Antibody concentration 15 ug/ml](http://images.novusbio.com/fullsize/LIS1-Antibody-5A5-Immunocytochemistry-Immunofluorescence-H00005048-M03-img0002.jpg)
![Sandwich ELISA: LIS1 Antibody (5A5) [H00005048-M03] - Detection limit for recombinant GST tagged PAFAH1B1 is 0.03 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/LIS1-Antibody-5A5-Sandwich-ELISA-H00005048-M03-img0003.jpg)