| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-68281SS | |||
| Azide and BSA Free | NBP2-80993 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87498-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38231-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-1701-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-37979-20ul | |||
![Western Blot: TRF-1 Antibody (57-6) [NB110-68281] - MicroRNA-214-3p (miR-214) mimic in rat vascular smooth muscle cells (A7r5) suppressed angiogenesis and proliferation but promoted senescence. Representative western blots depicting TERF1, TERF2, and quaking expression in mimic NC or miR-214 mimic transfected cells. Image collected and cropped by CiteAb from the following publication (//molmed.biomedcentral.com/articles/10.1186/s10020-020-00167-1), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TRF-1-Antibody-57-6-Western-Blot-NB110-68281-img0012.jpg "Western Blot: TRF-1 Antibody (57-6) [NB110-68281] - MicroRNA-214-3p (miR-214) mimic in rat vascular smooth muscle cells (A7r5) suppressed angiogenesis and proliferation but promoted senescence. Representative western blots depicting TERF1, TERF2, and quaking expression in mimic NC or miR-214 mimic transfected cells. Image collected and cropped by CiteAb from the following publication (//molmed.biomedcentral.com/articles/10.1186/s10020-020-00167-1), licensed under a CC-BY license.")
![Flow Cytometry: TRF-1 Antibody (57-6) [NB110-68281] - An intracellular stain was performed on HeLa with NB110-68281 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0101B, R&D Systems).](http://images.novusbio.com/fullsize/TRF-1-Antibody-57-6-Flow-Cytometry-NB110-68281-img0011.jpg)
![Western Blot: TRF-1 Antibody (57-6) [NB110-68281] - Detection of TRF1 in HeLa whole cell extracts.](http://images.novusbio.com/fullsize/TRF-1-Antibody-57-6-Western-Blot-NB110-68281-img0003.jpg)
![Western Blot: TRF-1 Antibody (57-6) [NB110-68281] - Detection of TRF1. 50 ug of total lysate each lane.](http://images.novusbio.com/fullsize/TRF-1-Antibody-57-6-Western-Blot-NB110-68281-img0005.jpg)
![Flow Cytometry: TRF-1 Antibody (57-6) [NB110-68281] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using TRF1 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.](http://images.novusbio.com/fullsize/TRF-1-Antibody-57-6-Flow-Cytometry-NB110-68281-img0009.jpg)
![Simple Western: TRF-1 Antibody (57-6) [NB110-68281] - Image shows a specific band for TRF1 in 0.5 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/TRF-1-Antibody-57-6-Simple-Western-NB110-68281-img0010.jpg)
![Western Blot: TRF-1 Antibody (57-6) [NB110-68281] - MicroRNA-214-3p (miR-214) antagomiR in rat vascular smooth muscle cells (A7r5) induced angiogenesis & proliferation but suppressed senescence. a Schematic of the protocol for transfection of A7r5 VSMCs with a miRNA antagomiR control (antagomiR NC) or a miR-214 antagomiR & processed at the indicated times. b The effects of miR-214 antagomiR on VSMC cell proliferation were tested by CCK-8 assay. c Angiogenetic mRNA expression levels of NOS3, VEGFA, CXCL12 & CXCR4 compared in cells transfected with antagomiR NC or miR-214 antagomiRs (n = 3). d Senescence-associated mRNA expression of TERT, TERF1 & TERF2 compared in cells transfected with antagomiR NC or miR-214 antagomiRs (n = 3). e, G Representative western blots depicting TERF1, TERF2, p16INK4, p21CIP1, pRB, & quaking expression in antagomiR NC or miR-214 antagomiRs transfected cells. f, h Normalized expression of TERF1, TERF2, p16INK4, p21CIP1, pRB, & quaking (n = 3). i Senescence-associated beta -galactosidase staining demonstrating senescence in SCR or miR-214 transfected cells. j Bar graphs show quantification of relative of SA-beta -gal positive cells (n = 3). Data are presented as the means ± SEM. * P < 0.05; ** P < 0.01; ns, non-significance (Two-tailed Student’s t-test) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32410577), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-68281_mouse-monoclonal-trf-1-antibody-57-6-310202416212346.jpg)
![Immunohistochemistry-Paraffin: Flotillin-1 Antibody [NBP1-87498] -Analysis in human adrenal gland and skin tissues using NBP1-87498 antibody. Corresponding FLOT1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/antibody/nbp1-87498_rabbit-polyclonal-flotillin-1-antibody-immunohistochemistry-paraffin-2472024125914..jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Flotillin-1 Antibody [NBP1-87498] -Analysis in human adrenal gland and skin tissues using NBP1-87498 antibody. Corresponding FLOT1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: Flotillin-1 Antibody [NBP1-87498] - Analysis in human cell lines SK-MEL-30 and MCF-7 using Anti-FLOT1 antibody. Corresponding FLOT1 RNA-seq data are presented for the same cell lines. Loading control: Anti-PFN1.](http://images.novusbio.com/fullsize/Flotillin-1-Antibody-Western-Blot-NBP1-87498-img0012.jpg)
![Western Blot: Flotillin-1 Antibody [NBP1-87498] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/Flotillin-1-Antibody-Western-Blot-NBP1-87498-img0009.jpg)
![Immunohistochemistry-Paraffin: Flotillin-1 Antibody [NBP1-87498] - Staining of human adrenal gland shows high expression.](http://images.novusbio.com/fullsize/Flotillin-1-Antibody-Immunohistochemistry-NBP1-87498-img0013.jpg)
![Immunohistochemistry-Paraffin: Flotillin-1 Antibody [NBP1-87498] -Staining of human Adrenal gland shows strong membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/antibody/nbp1-87498_rabbit-polyclonal-flotillin-1-antibody-immunohistochemistry-paraffin-247202413032..jpg)
![Immunohistochemistry-Paraffin: Flotillin-1 Antibody [NBP1-87498] - Staining of human Fallopian tube shows strong membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/antibody/nbp1-87498_rabbit-polyclonal-flotillin-1-antibody-immunohistochemistry-paraffin-247202413122..jpg)
![Immunohistochemistry-Paraffin: Flotillin-1 Antibody [NBP1-87498] - Staining of human Stomach shows strong membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/antibody/nbp1-87498_rabbit-polyclonal-flotillin-1-antibody-immunohistochemistry-paraffin-247202413216..jpg)
![Immunohistochemistry-Paraffin: Flotillin-1 Antibody [NBP1-87498] -Staining of human Skin shows very weak membranous positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/antibody/nbp1-87498_rabbit-polyclonal-flotillin-1-antibody-immunohistochemistry-paraffin-24720241338..jpg)
![Western Blot: Flotillin-1 Antibody [NBP3-38231] - Western blot analysis of various lysates, using [KO Validated] Flotillin-1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.](http://images.novusbio.com/fullsize/nbp3-38231_rabbit-flotillin-1-pab-101220241252844.jpg "Western Blot: Flotillin-1 Antibody [NBP3-38231] - Western blot analysis of various lysates, using [KO Validated] Flotillin-1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.")
![Immunoprecipitation: Flotillin-1 Antibody [NBP3-38231] - Immunoprecipitation analysis of 300 ug extracts of HeLa cells using 3 ug Flotillin-1 antibody. Western blot was performed from the immunoprecipitate using Flotillin-1 antibody at a dilution of 1:1000.](http://images.novusbio.com/fullsize/nbp3-38231_rabbit-flotillin-1-pab-1012202411545540.jpg)
![Immunohistochemistry: Flotillin-1 Antibody [NBP3-38231] - Immunohistochemistry analysis of paraffin-embedded Mouse kidney using [KO Validated] Flotillin-1 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38231_rabbit-flotillin-1-pab-10122024121097.jpg)
![Immunohistochemistry: Flotillin-1 Antibody [NBP3-38231] - Immunohistochemistry analysis of paraffin-embedded Rat heart using [KO Validated] Flotillin-1 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38231_rabbit-flotillin-1-pab-101220241252843.jpg)
![Immunocytochemistry/ Immunofluorescence: Flotillin-1 Antibody [NBP3-38231] - Immunofluorescence analysis of NIH/3T3 cells using Flotillin-1 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38231_rabbit-flotillin-1-pab-10122024121062.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Flotillin-1 Antibody [NBP3-38231] - Western Blot analysis of lysates from wild type (WT) and Flotillin-1 knockout (KO) 293T cells, using [KO Validated] Flotillin-1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.](http://images.novusbio.com/fullsize/nbp3-38231_rabbit-flotillin-1-pab-311202516593618.jpg)
![Western Blot: Flotillin-1 Antibody [NB100-1043] - Staining of K562 lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Detected by Chemiluminescence.](http://images.novusbio.com/fullsize/Flotillin-1-Antibody-Western-Blot-NB100-1043-img0001.jpg "Western Blot: Flotillin-1 Antibody [NB100-1043] - Staining of K562 lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Detected by Chemiluminescence.")
![Immunohistochemistry-Paraffin: Flotillin-1 Antibody [NB100-1043] - Negative Control showing staining of paraffin embedded Human Kidney, with no primary antibody.](http://images.novusbio.com/fullsize/Flotillin-1-Antibody-Immunohistochemistry-Paraffin-NB100-1043-img0003.jpg)
![Immunohistochemistry-Paraffin: Flotillin-1 Antibody [NB100-1043] - Staining of paraffin embedded Human Kidney. Antibody at 5 ug/mL. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.](http://images.novusbio.com/fullsize/Flotillin-1-Antibody-Immunohistochemistry-Paraffin-NB100-1043-img0002.jpg)
![Western Blot: ERF Antibody [NBP2-16366] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with ERF antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/ERF-Antibody-Western-Blot-NBP2-16366-img0008.jpg "Western Blot: ERF Antibody [NBP2-16366] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with ERF antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Western Blot: ERF Antibody [NBP2-16366] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ERF antibody diluted at 1:4000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/ERF-Antibody-Western-Blot-NBP2-16366-img0006.jpg)
![Immunohistochemistry-Paraffin: ERF Antibody [NBP2-16366] - U373 xenograft, using ERF antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/ERF-Antibody-Immunohistochemistry-Paraffin-NBP2-16366-img0002.jpg)
![Western Blot: Flotillin-1 Antibody [NBP1-79022] - Whole cell lysate from HeLa (15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-Flotillin-1 antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. Flotillin-1 was also immunoprecipitated by rabbit anti-Flotillin-1 antibody NBP1-79021 which recognizes an upstream epitope.](http://images.novusbio.com/fullsize/Flotillin-1-Antibody-Western-Blot-NBP1-79022-img0002.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: Flotillin-1 Antibody [NBP1-79022] - Whole cell lysate from HeLa (15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-Flotillin-1 antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. Flotillin-1 was also immunoprecipitated by rabbit anti-Flotillin-1 antibody NBP1-79021 which recognizes an upstream epitope.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Simple Western: Flotillin-1 Antibody [NBP1-79022] - Simple Western lane view shows lysates of Exosome Standards (A549 cell line) (NBP3-11645), exosome standards (HT29 cell line) (NBP3-11685), and COLO 205 human colorectal adenocarcinoma cell line whole cell lysate (WCL), loaded at 0.2 mg/mL. A specific band was detected for Flotillin-1 at approximately 51 kDa (as indicated) using 1:250 ug/mL of Flotillin-1 antibody (NBP1-79022). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Flotillin-1-Antibody-Simple-Western-NBP1-79022-img0003.jpg)
![Western Blot: Flotillin-1 Antibody (8I2S7) [NBP3-16152] - Analysis of extracts of various cell lines, using Flotillin-1 Rabbit mAb (NBP3-16152) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/Flotillin-1-Antibody-8I2S7-Western-Blot-NBP3-16152-img0004.jpg "Western Blot: Flotillin-1 Antibody (8I2S7) [NBP3-16152] - Analysis of extracts of various cell lines, using Flotillin-1 Rabbit mAb (NBP3-16152) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Western Blot: Flotillin-1 Antibody (8I2S7) [NBP3-16152] - Analysis of extracts of various cell lines, using Flotillin-1 Rabbit mAb (NBP3-16152) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/Flotillin-1-Antibody-8I2S7-Western-Blot-NBP3-16152-img0005.jpg)
![Immunohistochemistry: Flotillin-1 Antibody (8I2S7) [NBP3-16152] - Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using Flotillin-1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16152_rabbit-flotillin-1-mab-8i2s7-5220251215429.jpg)
![Immunohistochemistry: Flotillin-1 Antibody (8I2S7) [NBP3-16152] - Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using Flotillin-1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16152_rabbit-flotillin-1-mab-8i2s7-52202511365156.jpg)
![Immunohistochemistry: Flotillin-1 Antibody (8I2S7) [NBP3-16152] - Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer tissue using Flotillin-1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16152_rabbit-flotillin-1-mab-8i2s7-52202511365175.jpg)
![HCT-116 whole cell extract A. Control with 4 ug of preimmune Rabbit IgG B. Immunoprecipitation of Flotillin 1 protein by 4 ug Flotillin 1 antibody [C3], C-term 10 % SDS-PAGE The immunoprecipitated Flotillin 1 protein was detected by Flotillin 1 antibody [C3], C-term diluted at 1:1000. [EasyBlot anti-rabbit IgG was used as a secondary reagent]](http://images.novusbio.com/fullsize/Flotillin-1-Antibody-Immunoprecipitation-NBP2-16508-img0003.jpg "HCT-116 whole cell extract A. Control with 4 ug of preimmune Rabbit IgG B. Immunoprecipitation of Flotillin 1 protein by 4 ug Flotillin 1 antibody [C3], C-term 10 % SDS-PAGE The immunoprecipitated Flotillin 1 protein was detected by Flotillin 1 antibody [C3], C-term diluted at 1:1000. [EasyBlot anti-rabbit IgG was used as a secondary reagent]")
![Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Flotillin 1 antibody [C3], C-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/antibody/nbp2-16508_rabbit-polyclonal-flotillin-1-antibody-western-blot-8620239419.jpg)
![Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Flotillin 1 antibody [C3], C-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/antibody/nbp2-16508_rabbit-polyclonal-flotillin-1-antibody-western-blot-305202593537.png)
![Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Flotillin 1 antibody [C3], C-term (NBP2-16508) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-16508_rabbit-polyclonal-flotillin-1-antibody-western-blot-261120251953523.jpg)
![Western Blot: TRF-1 Antibody (TRF-78) [NB100-1701] - Cell line lysates were separated on SDS-PAGE and probed with 4 ug/mL Monoclonal Anti-TRF1 Clone: TRF-78. Lane 1: HeLa Nuclear, Lane 2: HeLa, Lane 3: HEK-293T, Lane 4: U2OS, Lane 5: HepG2, Lane 6: P19, Lane 7: PC12.](http://images.novusbio.com/fullsize/TRF-1-Antibody-TRF-78-Western-Blot-NB100-1701-img0007.jpg "Western Blot: TRF-1 Antibody (TRF-78) [NB100-1701] - Cell line lysates were separated on SDS-PAGE and probed with 4 ug/mL Monoclonal Anti-TRF1 Clone: TRF-78. Lane 1: HeLa Nuclear, Lane 2: HeLa, Lane 3: HEK-293T, Lane 4: U2OS, Lane 5: HepG2, Lane 6: P19, Lane 7: PC12.")
![Immunocytochemistry/Immunofluorescence: TRF-1 Antibody (TRF-78) - BSA Free [NB100-1701] - HeLa cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton X-100. Fixed cells were stained with 10 ug/mL Monoclonal Anti-TRF1 Clone: TRF-78. The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.](http://images.novusbio.com/fullsize/TRF-1-Antibody-TRF-78-BSA-Free-Immunocytochemistry-Immunofluorescence-NB100-1701-img0008.jpg)
![TRF1 antibody [4E4] detects TRF1 protein at telomere by immunofluorescent analysis.Sample: U2OS cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: TRF1 protein stained by TRF1 antibody [4E4] (NBP1-41217) diluted at 1:500.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp1-41217_mouse-monoclonal-trf-1-antibody-4e4-immunocytochemistry-immunofluorescence-261120251838405.jpg "TRF1 antibody [4E4] detects TRF1 protein at telomere by immunofluorescent analysis.Sample: U2OS cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: TRF1 protein stained by TRF1 antibody [4E4] (NBP1-41217) diluted at 1:500.Blue: Hoechst 33342 staining.")
![Western Blot: Flotillin-1 Antibody [NBP3-37979] - Western blot analysis of various lysates using Flotillin-1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-37979_rabbit-flotillin-1-pab-1012202411353061.jpg "Western Blot: Flotillin-1 Antibody [NBP3-37979] - Western blot analysis of various lysates using Flotillin-1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.")
![Immunohistochemistry: Flotillin-1 Antibody [NBP3-37979] - Immunohistochemistry analysis of paraffin-embedded Mouse pancreas using Flotillin-1 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-37979_rabbit-flotillin-1-pab-1012202411343364.jpg)
![Immunohistochemistry: Flotillin-1 Antibody [NBP3-37979] - Immunohistochemistry analysis of paraffin-embedded Rat heart using Flotillin-1 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-37979_rabbit-flotillin-1-pab-1012202411392922.jpg)
![Immunohistochemistry-Paraffin: ERF Antibody [NB100-88120] - FFPE section of human ovarian carcinoma (left) and mouse renal cell carcinoma (right). Antibody: Affinity purified rabbit anti-ERF (NB100-88120). Detection: DAB](http://images.novusbio.com/fullsize/ERF-Antibody-Immunohistochemistry-NB100-88120-img0004.jpg "Immunohistochemistry-Paraffin: ERF Antibody [NB100-88120] - FFPE section of human ovarian carcinoma (left) and mouse renal cell carcinoma (right). Antibody: Affinity purified rabbit anti-ERF (NB100-88120). Detection: DAB")
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: ERF Antibody [NB100-88120] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. NB100-88120 used for IP at 3 mcg/mg lysate. ERF was also immunoprecipitated by NB100-88119, which recognizes an upstream epitope.](http://images.novusbio.com/fullsize/ERF-Antibody-Western-Blot-NB100-88120-img0003.jpg)