| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-44513 | |||
| Azide and BSA Free | NBP2-34569-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89517-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-72427 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76843-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47778-0.1mg | |||
![Western Blot: B7-2/CD86 Antibody (BU63) [NBP2-25208] - Western blot shows lysates of Ramos human Burkitt's lymphoma parental cell line and B7-2 knockout (KO) Ramos cell line. PVDF membrane was probed with 1.0 ug/mL of Mouse Anti-Human B7-2 Monoclonal Antibody (Catalog # NBP2-25208) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for B7-2 at approximately 90 kDa (as indicated) in the parental Ramos cell line, but is not detectable in the knockout Ramos cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/B7-2-CD86-Antibody-BU63-Knockout-Validated-NBP2-25208-img0005.jpg "<b>Genetic Strategies Validation. </b>Western Blot: B7-2/CD86 Antibody (BU63) [NBP2-25208] - Western blot shows lysates of Ramos human Burkitt's lymphoma parental cell line and B7-2 knockout (KO) Ramos cell line. PVDF membrane was probed with 1.0 ug/mL of Mouse Anti-Human B7-2 Monoclonal Antibody (Catalog # NBP2-25208) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for B7-2 at approximately 90 kDa (as indicated) in the parental Ramos cell line, but is not detectable in the knockout Ramos cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry: B7-2/CD86 Antibody (BU63) [NBP2-25208] - Macrophage polarization states. Distribution and proportion of CD86+ macrophages (brown) were identified using IHC procedures. Intensity for the color response to antibodies was visible in lung tissues from all groups. Population proportions for the stained cells as shown by red arrows were calculated as a fold change of the control. (d). The results were expressed as Mean +/- SD (n = 3). **: a p-value of < 0.01 vs NS, OVA/BUD and OVA/PCI except the number of CD86+ cells in OVA/PCI group. #: P < 0.05 vs NS and ##; P < 0.01 vs either NS or OVA/BUD group. Image collected and cropped by CiteAb from the following publication (//respiratory-research.biomedcentral.com/articles/10.1186/s12931-020-1322-5) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/B7-2-CD86-Antibody-BU63-Immunohistochemistry-NBP2-25208-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: B7-2/CD86 Antibody (BU63) [NBP2-25208] - Immunofluorescence staining of PFA-fixed Ramos cells using followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Red Dot.](http://images.novusbio.com/fullsize/B7-2-CD86-Antibody-BU63-Immunocytochemistry-Immunofluorescence-NBP2-25208-img0006.jpg)
![Immunohistochemistry: B7-2/CD86 Antibody (BU63) [NBP2-25208] - Effects of 20(S)-Rg3 on macrophage polarization in atherosclerotic lesions of diabetic ApoE-/- mice. Co-immunofluorescence staining for macrophage (anti-Moma-2 antibody, green), M1 marker CD86 (red) and DAPI (blue) (n = 5, respectively). Scale bar: 20 um. Image collected and cropped by CiteAb from the following publication (//www.frontiersin.org/articles/10.3389/fphar.2018.00464/full) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/B7-2-CD86-Antibody-BU63-Immunohistochemistry-NBP2-25208-img0010.jpg)
![Flow Cytometry: B7-2/CD86 Antibody (BU63) [NBP2-25208] - Flow Cytometric Analysis of PFA-fixed Ramos cells. B7-2/CD86 Antibody (BU63) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/B7-2-CD86-Antibody-BU63-Flow-Cytometry-NBP2-25208-img0008.jpg)
![Immunohistochemistry-Paraffin: B7-2/CD86 Antibody (BU63) [NBP2-25208] - Formalin-fixed, paraffin-embedded esophagus tumor tissue stained with CD86 antibody (5 ug/ml), peroxidase-conjugate and DAB chromogen. Note strong staining of differentiated squamous cells. TMA was used for this test.](http://images.novusbio.com/fullsize/B7-2-CD86-Antibody-BU63-Immunohistochemistry-Paraffin-NBP2-25208-img0004.jpg)
![Flow Cytometry: B7-2/CD86 Antibody (BU63) [NBP2-25208] - Flow Cytometric Analysis of human PBMCs using B7-2/CD86 Antibody (BU63); Goat anti-Mouse IgG-CF488 (red); Isotype Control (green).](http://images.novusbio.com/fullsize/B7-2-CD86-Antibody-BU63-Flow-Cytometry-NBP2-25208-img0007.jpg)
![Immunohistochemistry: B7-2/CD86 Antibody (BU63) [NBP2-25208] - Macrophage polarization states. Distribution & proportion of CD68+ (a), CD86+ (c) & CD163+ (e) macrophages (brown) were identified using IHC procedures. Intensity for the color response to antibodies was visible in lung tissues from all groups. Population proportions for the stained cells as shown by red arrows were calculated as a fold change of the control. (b, d, f). The results were expressed as Mean ± SD (n = 3). **: a p-value of < 0.01 vs NS, OVA/BUD & OVA/PCI except the number of CD86+ cells in OVA/PCI group. #: P < 0.05 vs NS & ##; P < 0.01 vs either NS or OVA/BUD group Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32111211), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-25208_mouse-monoclonal-b7-2-cd86-antibody-bu63-imgenex-img-6882a-25ug-31020241534324.jpg)
![Immunocytochemistry/ Immunofluorescence: B7-2/CD86 Antibody (BU63) [NBP2-25208] - Effects of 20(S)-Rg3 on macrophage polarization in atherosclerotic lesions of diabetic ApoE–/– mice. (A,B) Co-immunofluorescence staining of the aortic root for macrophage (anti-Moma-2 antibody, green) & M1 marker iNos (red), & a bar graph summarizing the results (n = 5, respectively). (C,D) Co-immunofluorescence staining for macrophage (anti-Moma-2 antibody, green) & M1 marker CD86 (red), & a bar graph summarizing the results (n = 5, respectively). Scale bar: 20 μm. Data are mean ± SEM. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29867472), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-25208_mouse-monoclonal-b7-2-cd86-antibody-bu63-imgenex-img-6882a-25ug-31020241535650.jpg)
![M1 macrophages were induced in the in vivo mouse intervertebral disc (mIVD) degeneration model (puncture model). (a) Immunohistological analysis of Iba-1, iNOS, and arginase (brown staining) expression in nucleus pulposus (NP), annulus fibrosis (AF), and cartilage endplate (CEP) [right] at high magnification and in whole mIVDs (left) at low magnification. Images using an IgG isotype control antibody are included to demonstrate the specificity of the IHC staining. (b) Protein was extracted from punctured mIVDs in the puncture model, and western blotting was performed using antibodies against CD86, CD163, and beta -actin. (c) Images of the panel in (b), captured using a ChemiDoc Touch system and quantified using ImageJ. Biological and technical replicates were performed three times. Values represent the mean +/- SD. *p < 0.05, **p < 0.01, relative to the corresponding control. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/41053148), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-25208_mouse-monoclonal-b7-2-cd86-antibody-bu63-western-blot-13220261327102.jpg)
![Immunocytochemistry/Immunofluorescence: NUDC Antibody [NBP1-89517] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.](http://images.novusbio.com/fullsize/NUDC-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89517-img0007.jpg "Immunocytochemistry/Immunofluorescence: NUDC Antibody [NBP1-89517] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.")
![Immunohistochemistry-Paraffin: NUDC Antibody [NBP1-89517] - Staining of human prostate shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/NUDC-Antibody-Immunohistochemistry-Paraffin-NBP1-89517-img0021.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: NUDC Antibody [NBP1-89517] - Staining of human cerebral cortex, fallopian tube, prostate and testis using Anti-NUDC antibody NBP1-89517 (A) shows similar protein distribution across tissues to independent antibody NBP1-89510 (B).](http://images.novusbio.com/fullsize/NUDC-Antibody-Immunohistochemistry-Paraffin-NBP1-89517-img0019.jpg)
![Immunohistochemistry-Paraffin: NUDC Antibody [NBP1-89517] - Staining of human cerebral cortex shows weak to moderate cytoplasmic and nuclear positivity in neurons.](http://images.novusbio.com/fullsize/NUDC-Antibody-Immunohistochemistry-Paraffin-NBP1-89517-img0018.jpg)
![Immunohistochemistry-Paraffin: NUDC Antibody [NBP1-89517] - Staining of human Fallopian tube shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/NUDC-Antibody-Immunohistochemistry-Paraffin-NBP1-89517-img0020.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: NUDC Antibody [NBP1-89517] - Analysis using Anti-NUDC antibody NBP1-89517 (A) shows similar pattern to independent antibody NBP1-89510 (B).](http://images.novusbio.com/fullsize/NUDC-Antibody-Western-Blot-NBP1-89517-img0013.jpg)
![Immunohistochemistry-Paraffin: BAFF/BLyS/TNFSF13B Antibody (Buffy-2) [NBP1-97622] - Human skin with marked lymphocyte infiltration. Lymphocytes and lymphatic vessels were membranous or cytoplasmic positive for BAFF. Nuclei of keratinocytes were also stained. Buffy2 (working dilution 2ug/mL). detected with Leica BOND Refine polymer (DAB) kit. Rabbit ant-rat IgG+M as a linker antibody pretreatment: basic solution (BOND ER2). IHC-P image submitted by a verified customer review.](http://images.novusbio.com/fullsize/BAFF-BLyS-TNFSF13B-Antibody-Buffy-2-Immunohistochemistry-Paraffin-NBP1-97622-img0002.jpg "Immunohistochemistry-Paraffin: BAFF/BLyS/TNFSF13B Antibody (Buffy-2) [NBP1-97622] - Human skin with marked lymphocyte infiltration. Lymphocytes and lymphatic vessels were membranous or cytoplasmic positive for BAFF. Nuclei of keratinocytes were also stained. Buffy2 (working dilution 2ug/mL). detected with Leica BOND Refine polymer (DAB) kit. Rabbit ant-rat IgG+M as a linker antibody pretreatment: basic solution (BOND ER2). IHC-P image submitted by a verified customer review.")
![Immunohistochemistry-Paraffin: TNFSF13B Antibody (Buffy-2) [NBP1-97622] - Staining of paraffin section of synovial tissue from a patient with Rheumatoid Arthritis. Arrows indicate vessels (20x) staining positive for BAFF using Buffy-2. Method: Tissues are in paraffin sections. Sections are de-waxed and rehydrated, then cooked. Sections are stained in wet boxes, rehydrated and finally prepared as coverslip slides using Eukitt solution.](http://images.novusbio.com/fullsize/TNFSF13B-Antibody-Buffy-2-Immunohistochemistry-Paraffin-NBP1-97622-img0001.jpg)
![Western Blot: B7-2/CD86 Antibody (BLR030F) [NBP2-76417] - Whole cell lysate (10 µg) from HEK293T, HDLM-2, Jurkat,MJ, and HeLa cells prepared using NETN lysis buffer.Antibody: Rabbit anti-CD86 recombinant monoclonalantibody [BLR030F] used at 1:1000.Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with anexposure time of 10 seconds.](http://images.novusbio.com/fullsize/nbp2-76417_rabbit-monoclonal-b7-2-cd86-antibody-blr030f-western-blot-1311202411142..png "Western Blot: B7-2/CD86 Antibody (BLR030F) [NBP2-76417] - Whole cell lysate (10 µg) from HEK293T, HDLM-2, Jurkat,MJ, and HeLa cells prepared using NETN lysis buffer.Antibody: Rabbit anti-CD86 recombinant monoclonalantibody [BLR030F] used at 1:1000.Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with anexposure time of 10 seconds.")
![Flow Cytometry: B7-2/CD86 Antibody (BLR030F) [NBP2-76417] - Antibody: Rabbit anti-CD86 recombinantmonoclonal [BLR030F] or isotype control(unshaded). Secondary: DyLight® 488-conjugated goatanti-rabbit IgG.](http://images.novusbio.com/fullsize/nbp2-76417_rabbit-monoclonal-b7-2-cd86-antibody-blr030f-flow-cytometry-1311202411333..png)
![Immunohistochemistry-Paraffin: B7-2/CD86 Antibody (BLR030F) [NBP2-76417] -FFPE section of tonsil. Antibody: Rabbit anti-CD86recombinant monoclonal antibody [BLR030F]. Secondary: HRP-conjugated goat anti-rabbit IgG.](http://images.novusbio.com/fullsize/nbp2-76417_rabbit-monoclonal-b7-2-cd86-antibody-blr030f-immunohistochemistry-paraffin-131120241151..png)
![Immunocytochemistry/Immunofluorescence: B7-2/CD86 Antibody (BLR030F) [NBP2-76417] -Section of HDLM-2 cells. Antibody: Rabbit anti-CD86recombinant monoclonal antibody [BLR030F]. Secondary: HRP-conjugated goat anti-rabbit IgG.](http://images.novusbio.com/fullsize/nbp2-76417_rabbit-monoclonal-b7-2-cd86-antibody-blr030f-immunocytochemistry-immunofluorescence-1311202411712..png)
![Immunoprecipitation: B7-2/CD86 Antibody (BLR030F) [NBP2-76417] - Samples: Whole cell lysate (1 mg per IP; 10% of IP loaded) from HDLM-2 cells prepared using NETN lysis buffer. Antibodies: NBP2-76417 Lot 4 used for immunoprecipitation at 6 ul per reaction. B7-2/CD86 was also immunoprecipitated by a previous lot of this antibody (NBP2-76417 Lot 1) and a second antibody, NBP2-76416, against a different epitope of B7-2/CD86 (BLR029F). For blotting immunoprecipitated CD86, NBP2-76417 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 1 second.](http://images.novusbio.com/fullsize/nbp2-76417_rabbit-monoclonal-b7-2-cd86-antibody-blr030f-immunoprecipitation-1312025145029..jpg)
![Immunoprecipitation: B7-2/CD86 Antibody (BLR030F) [NBP2-76417] - Detection of human CD86 by WB of immunoprecipitates from HDML-2 lysate. Antibodies: Rabbit anti-CD86 recombinant monoclonal [BLR030F] (NBP2-76417) and rabbit anti-CD86 recombinant monoclonal [BLR029F] (NBP2-76416).](http://images.novusbio.com/fullsize/nbp2-76417_rabbit-monoclonal-b7-2-cd86-antibody-blr030f-immunoprecipitation-131202515750..jpg)
![Immunocytochemistry/Immunofluorescence: NUDC Antibody [NBP1-89510] - Immunofluorescent staining of human cell line A-431 shows localization to cytosol.](http://images.novusbio.com/fullsize/NUDC-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89510-img0006.jpg "Immunocytochemistry/Immunofluorescence: NUDC Antibody [NBP1-89510] - Immunofluorescent staining of human cell line A-431 shows localization to cytosol.")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: NUDC Antibody [NBP1-89510] - Staining of human cerebral cortex, fallopian tube, prostate and testis using Anti-NUDC antibody NBP1-89510 (A) shows similar protein distribution across tissues to independent antibody NBP1-89517 (B).](http://images.novusbio.com/fullsize/NUDC-Antibody-Immunohistochemistry-Paraffin-NBP1-89510-img0017.jpg)
![Immunohistochemistry-Paraffin: NUDC Antibody [NBP1-89510] - Staining of human testis shows strong cytoplasmic and nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/NUDC-Antibody-Immunohistochemistry-Paraffin-NBP1-89510-img0020.jpg)
![Immunohistochemistry-Paraffin: NUDC Antibody [NBP1-89510] - Staining of human cerebral cortex shows weak to moderate cytoplasmic and nuclear positivity in neurons.](http://images.novusbio.com/fullsize/NUDC-Antibody-Immunohistochemistry-Paraffin-NBP1-89510-img0015.jpg)
![Immunohistochemistry-Paraffin: NUDC Antibody [NBP1-89510] - Staining of human Fallopian tube shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/NUDC-Antibody-Immunohistochemistry-Paraffin-NBP1-89510-img0018.jpg)
![Immunohistochemistry-Paraffin: NUDC Antibody [NBP1-89510] - Staining of human prostate shows weak cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/NUDC-Antibody-Immunohistochemistry-Paraffin-NBP1-89510-img0019.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: NUDC Antibody [NBP1-89510] - Analysis using Anti-NUDC antibody NBP1-89510 (A) shows similar pattern to independent antibody NBP1-89517 (B).](http://images.novusbio.com/fullsize/NUDC-Antibody-Western-Blot-NBP1-89510-img0011.jpg)
![Western Blot: BTLA/CD272 Antibody (2E4) [NBP2-45549] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BTLA.](http://images.novusbio.com/fullsize/BTLA-CD272-Antibody-2E4-Western-Blot-NBP2-45549-img0015.jpg "Western Blot: BTLA/CD272 Antibody (2E4) [NBP2-45549] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BTLA.")
![Immunohistochemistry: BTLA/CD272 Antibody (2E4) [NBP2-45549] - Analysis of Carcinoma of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/BTLA-CD272-Antibody-2E4-Immunohistochemistry-NBP2-45549-img0014.jpg)
![Flow Cytometry: BTLA/CD272 Antibody (2E4) [NBP2-45549] - Analysis of live Sup-B15 cells, using BTLA antibody , compared to IgG1 isotype control (Black).](http://images.novusbio.com/fullsize/BTLA-CD272-Antibody-2E4-Flow-Cytometry-NBP2-45549-img0001.jpg)
![Immunohistochemistry: BTLA/CD272 Antibody (2E4) [NBP2-45549] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/BTLA-CD272-Antibody-2E4-Immunohistochemistry-NBP2-45549-img0002.jpg)
![Immunohistochemistry: BTLA/CD272 Antibody (2E4) [NBP2-45549] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/BTLA-CD272-Antibody-2E4-Immunohistochemistry-NBP2-45549-img0003.jpg)
![Immunohistochemistry: BTLA/CD272 Antibody (2E4) [NBP2-45549] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/BTLA-CD272-Antibody-2E4-Immunohistochemistry-NBP2-45549-img0004.jpg)
![Immunohistochemistry: BTLA/CD272 Antibody (2E4) [NBP2-45549] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/BTLA-CD272-Antibody-2E4-Immunohistochemistry-NBP2-45549-img0005.jpg)
![Immunohistochemistry: BTLA/CD272 Antibody (2E4) [NBP2-45549] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/BTLA-CD272-Antibody-2E4-Immunohistochemistry-NBP2-45549-img0006.jpg)
![Immunohistochemistry: BTLA/CD272 Antibody (2E4) [NBP2-45549] - Analysis of Carcinoma of Human kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/BTLA-CD272-Antibody-2E4-Immunohistochemistry-NBP2-45549-img0007.jpg)
![Immunohistochemistry: BTLA/CD272 Antibody (2E4) [NBP2-45549] - Analysis of Carcinoma of Human liver tissue.(Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/BTLA-CD272-Antibody-2E4-Immunohistochemistry-NBP2-45549-img0008.jpg)
![Immunohistochemistry: BTLA/CD272 Antibody (2E4) [NBP2-45549] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/BTLA-CD272-Antibody-2E4-Immunohistochemistry-NBP2-45549-img0009.jpg)
![Immunohistochemistry: BTLA/CD272 Antibody (2E4) [NBP2-45549] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/BTLA-CD272-Antibody-2E4-Immunohistochemistry-NBP2-45549-img0010.jpg)
![Immunohistochemistry: BTLA/CD272 Antibody (2E4) [NBP2-45549] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/BTLA-CD272-Antibody-2E4-Immunohistochemistry-NBP2-45549-img0011.jpg)
![Immunohistochemistry: BTLA/CD272 Antibody (2E4) [NBP2-45549] - Analysis of Carcinoma of Human lung tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/BTLA-CD272-Antibody-2E4-Immunohistochemistry-NBP2-45549-img0012.jpg)
![Immunohistochemistry: BTLA/CD272 Antibody (2E4) [NBP2-45549] - Analysis of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/BTLA-CD272-Antibody-2E4-Immunohistochemistry-NBP2-45549-img0013.jpg)
![Western Blot: BTLA/CD272 Antibody (OTI2E4) - Azide and BSA Free [NBP2-72427] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BTLA.](http://images.novusbio.com/fullsize/BTLA-CD272-Antibody-OTI2E4-Azide-and-BSA-Free-Western-Blot-NBP2-72427-img0001.jpg "Western Blot: BTLA/CD272 Antibody (OTI2E4) - Azide and BSA Free [NBP2-72427] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BTLA.")
![Immunohistochemistry: BTLA/CD272 Antibody (OTI2E4) - Azide and BSA Free [NBP2-72427] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/BTLA-CD272-Antibody-OTI2E4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72427-img0014.jpg)
![Flow Cytometry: BTLA/CD272 Antibody (OTI2E4) - Azide and BSA Free [NBP2-72427] - Analysis of live Sup-B15 cells, using BTLA antibody , compared to IgG1 isotype control (Black).](http://images.novusbio.com/fullsize/BTLA-CD272-Antibody-OTI2E4-Azide-and-BSA-Free-Flow-Cytometry-NBP2-72427-img0002.jpg)
![Immunohistochemistry: BTLA/CD272 Antibody (OTI2E4) - Azide and BSA Free [NBP2-72427] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/BTLA-CD272-Antibody-OTI2E4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72427-img0003.jpg)
![Immunohistochemistry: BTLA/CD272 Antibody (OTI2E4) - Azide and BSA Free [NBP2-72427] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/BTLA-CD272-Antibody-OTI2E4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72427-img0004.jpg)
![Immunohistochemistry: BTLA/CD272 Antibody (OTI2E4) - Azide and BSA Free [NBP2-72427] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/BTLA-CD272-Antibody-OTI2E4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72427-img0005.jpg)
![Immunohistochemistry: BTLA/CD272 Antibody (OTI2E4) - Azide and BSA Free [NBP2-72427] - Analysis of Carcinoma of Human kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/BTLA-CD272-Antibody-OTI2E4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72427-img0006.jpg)
![Immunohistochemistry: BTLA/CD272 Antibody (OTI2E4) - Azide and BSA Free [NBP2-72427] - Analysis of Carcinoma of Human liver tissue.(Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/BTLA-CD272-Antibody-OTI2E4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72427-img0007.jpg)
![Immunohistochemistry: BTLA/CD272 Antibody (OTI2E4) - Azide and BSA Free [NBP2-72427] - Analysis of Carcinoma of Human lung tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/BTLA-CD272-Antibody-OTI2E4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72427-img0008.jpg)
![Immunohistochemistry: BTLA/CD272 Antibody (OTI2E4) - Azide and BSA Free [NBP2-72427] - Analysis of Carcinoma of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/BTLA-CD272-Antibody-OTI2E4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72427-img0009.jpg)
![Immunohistochemistry: BTLA/CD272 Antibody (OTI2E4) - Azide and BSA Free [NBP2-72427] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/BTLA-CD272-Antibody-OTI2E4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72427-img0010.jpg)
![Immunohistochemistry: BTLA/CD272 Antibody (OTI2E4) - Azide and BSA Free [NBP2-72427] - Analysis of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/BTLA-CD272-Antibody-OTI2E4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72427-img0011.jpg)
![Immunohistochemistry: BTLA/CD272 Antibody (OTI2E4) - Azide and BSA Free [NBP2-72427] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/BTLA-CD272-Antibody-OTI2E4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72427-img0012.jpg)
![Immunohistochemistry: BTLA/CD272 Antibody (OTI2E4) - Azide and BSA Free [NBP2-72427] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/BTLA-CD272-Antibody-OTI2E4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72427-img0013.jpg)
![Immunohistochemistry: BTLA/CD272 Antibody (OTI2E4) - Azide and BSA Free [NBP2-72427] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/BTLA-CD272-Antibody-OTI2E4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72427-img0015.jpg)
![Western Blot: 14-3-3 zeta Antibody [NBP2-24593] - analysis of 14-3-3 zeta in human brain lysate in the 1) absence, 2) presence of immunizing peptide, 3) mouse brain lysate and 4) rat brain lysate in the absence of immunizing peptide using this antibody. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/14-3-3-zeta-Antibody-Western-Blot-NBP2-24593-img0001.jpg "Western Blot: 14-3-3 zeta Antibody [NBP2-24593] - analysis of 14-3-3 zeta in human brain lysate in the 1) absence, 2) presence of immunizing peptide, 3) mouse brain lysate and 4) rat brain lysate in the absence of immunizing peptide using this antibody. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Immunohistochemistry-Paraffin: 14-3-3 zeta Antibody [NBP2-24593] - Formalin-fixed, paraffin-embedded human spleen stained with 14-3-3 zeta antibody at 10 ug/ml. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.](http://images.novusbio.com/fullsize/14-3-3-zeta-Antibody-Immunohistochemistry-Paraffin-NBP2-24593-img0002.jpg)
![Western Blot: 14-3-3 zeta Antibody [NBP1-31325] - Sample(30 ug of whole cell lysate) A:Hep G2 B:MOLT4 12% SDS PAGE, antibody diluted at 1:2000.](http://images.novusbio.com/fullsize/14-3-3-zeta-Antibody-Western-Blot-NBP1-31325-img0005.jpg "Western Blot: 14-3-3 zeta Antibody [NBP1-31325] - Sample(30 ug of whole cell lysate) A:Hep G2 B:MOLT4 12% SDS PAGE, antibody diluted at 1:2000.")
![Immunohistochemistry-Paraffin: 14-3-3 zeta Antibody [NBP1-31325] - Mouse colon. 14-3-3 zeta antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/14-3-3-zeta-Antibody-Immunohistochemistry-Paraffin-NBP1-31325-img0006.jpg)
![Immunohistochemistry-Paraffin: 14-3-3 zeta Antibody [NBP1-31325] - Cal27 xenograft, using 14-3-3 zeta antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/14-3-3-zeta-Antibody-Immunohistochemistry-Paraffin-NBP1-31325-img0003.jpg)
![Western Blot: 14-3-3 zeta Antibody [NBP1-31325] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with 14-3-3 zeta antibody (NBP1-31325) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31325_rabbit-polyclonal-14-3-3-zeta-antibody-91020242013121.jpg)
![Western Blot: 14-3-3 zeta Antibody [NBP1-31325] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with 14-3-3 zeta antibody (NBP1-31325) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31325_rabbit-polyclonal-14-3-3-zeta-antibody-91020242075967.jpg)
![Western Blot: BAFF/BLyS/TNFSF13B Antibody [NBP1-76843] - Western Blot Validation with Recombinant Protein. Loading: 30 ng of human BAFF recombinant protein per lane. Antibodies: BAFF/BLyS/TNFSF13B NBP1-76843 (Lane 1: 0.25 ug/mL; Lane 2: 0.5 ug/mL and Lane 3: 1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Observed at around 18kDa.](http://images.novusbio.com/fullsize/BAFF-BLyS-TNFSF13B-Antibody-Western-Blot-NBP1-76843-img0005.jpg "<b>Expression of Tagged Proteins Validation. </b>Western Blot: BAFF/BLyS/TNFSF13B Antibody [NBP1-76843] - Western Blot Validation with Recombinant Protein. Loading: 30 ng of human BAFF recombinant protein per lane. Antibodies: BAFF/BLyS/TNFSF13B NBP1-76843 (Lane 1: 0.25 ug/mL; Lane 2: 0.5 ug/mL and Lane 3: 1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Observed at around 18kDa.")
![<b>Expression of Tagged Proteins Validation. </b>Western Blot: BAFF/BLyS/TNFSF13B Antibody [NBP1-76843] - Western Blot Validation in Human, Mouse and Rat Cell Lines. Loading: 15 ug of lysates per lane. Antibodies: BAFF/BLyS/TNFSF13B NBP1-76843 (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/BAFF-BLyS-TNFSF13B-Antibody-Western-Blot-NBP1-76843-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: BAFF/BLyS/TNFSF13B Antibody - BSA Free [NBP1-76843] - Validation of BAFF/BLyS/TNFSF13B in HL60 Cells. Immunocytochemical analysis of HL60 cells using anti-BAFF/BLyS/TNFSF13B antibody at 1 ug/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-76843_rabbit-polyclonal-baff-blys-tnfsf13b-antibody-271120242010580.jpg)
![Western Blot: BAFF/BLyS/TNFSF13B Antibody - BSA Free [NBP1-76843] - Validation in Human HL60 Cell Lysate (H) and Mouse Spleen Lysate (M). Loading: 15 ug of lysates per lane.Antibodies: BAFF/BLyS/TNFSF13B (1 u/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/nbp1-76843_rabbit-polyclonal-baff-blys-tnfsf13b-antibody-2711202420285892.jpg)
![Immunocytochemistry/ Immunofluorescence: BAFF/BLyS/TNFSF13B Antibody - BSA Free [NBP1-76843] - Validation of BAFF/BLyS/TNFSF13B in Human Spleen Tissue. Immunofluorescent analysis of 4% paraformaldehyde-fixed human spleen tissue labeling BAFF/BLyS/TNFSF13B with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).](http://images.novusbio.com/fullsize/nbp1-76843_rabbit-polyclonal-baff-blys-tnfsf13b-antibody-2711202420285848.gif)
![Western Blot: B7-2/CD86 Antibody (C86/1146) [NBP2-44514] - Western blot shows lysates of Ramos human Burkitt's lymphoma parental cell line and B7-2 knockout (KO) Ramos cell line. PVDF membrane was probed with 1.0 ug/mL of Mouse Anti-Human B7-2 Monoclonal Antibody (Catalog # NBP2-44514) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for B7-2 at approximately 90 kDa (as indicated) in the parental Ramos cell line, but is not detectable in the knockout Ramos cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/B7-2-CD86-Antibody-C86-1146-Knockout-Validated-NBP2-44514-img0006.jpg "<b>Genetic Strategies Validation. </b>Western Blot: B7-2/CD86 Antibody (C86/1146) [NBP2-44514] - Western blot shows lysates of Ramos human Burkitt's lymphoma parental cell line and B7-2 knockout (KO) Ramos cell line. PVDF membrane was probed with 1.0 ug/mL of Mouse Anti-Human B7-2 Monoclonal Antibody (Catalog # NBP2-44514) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for B7-2 at approximately 90 kDa (as indicated) in the parental Ramos cell line, but is not detectable in the knockout Ramos cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: B7-2/CD86 Antibody (C86/1146) [NBP2-44514] - Analysis of Daudi Cell Lysate using CD86 Monoclonal Antibody (C86/1146).](http://images.novusbio.com/fullsize/B7-2-CD86-Antibody-C86-1146-Western-Blot-NBP2-44514-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: B7-2/CD86 Antibody (C86/1146) [NBP2-44514] - Confocal Immunofluorescence image of Raji cells using B7-2/CD86 Antibody (C86/1146) followed by goat anti-Mouse IgG conjugated with CF488 (green). Red Dot is used to label the Nuclei (red).](http://images.novusbio.com/fullsize/B7-2-CD86-Antibody-C86-1146-Immunocytochemistry-Immunofluorescence-NBP2-44514-img0007.jpg)
![Immunohistochemistry-Paraffin: B7-2/CD86 Antibody (C86/1146) [NBP2-44514] - Human Melanoma stained with CD86 Monoclonal Antibody (C86/1146).](http://images.novusbio.com/fullsize/B7-2-CD86-Antibody-C86-1146-Immunohistochemistry-Paraffin-NBP2-44514-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: RNF7 Antibody [NBP1-85594] - Staining of human cell line A-431 shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/RNF7-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85594-img0007.jpg "Immunocytochemistry/Immunofluorescence: RNF7 Antibody [NBP1-85594] - Staining of human cell line A-431 shows localization to nucleoplasm. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: RNF7 Antibody [NBP1-85594] -Staining of human kidney shows moderate cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/antibody/nbp1-85594_rabbit-polyclonal-rnf7-antibody-immunohistochemistry-paraffin-3052024122956..jpg)
![Immunohistochemistry-Paraffin: RNF7 Antibody [NBP1-85594] -Staining of human pancreas shows weak cytoplasmic positivity in exocrine glandular cells.](http://images.novusbio.com/fullsize/antibody/nbp1-85594_rabbit-polyclonal-rnf7-antibody-immunohistochemistry-paraffin-3052024123055..jpg)
![Immunohistochemistry-Paraffin: RNF7 Antibody [NBP1-85594] - Staining of human lymph node shows very weak positivity in germinal center cells as expected.](http://images.novusbio.com/fullsize/antibody/nbp1-85594_rabbit-polyclonal-rnf7-antibody-immunohistochemistry-paraffin-3052024123157..jpg)
