| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-06407-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-97836-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-06301-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-97009-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-97772-50ul | |||
![Western Blot: KIFC1 Antibody [NBP3-06407] - KIFC1 Antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Observed band size:74 kDa.](http://images.novusbio.com/fullsize/KIFC1-Antibody-Western-Blot-NBP3-06407-img0001.jpg "Western Blot: KIFC1 Antibody [NBP3-06407] - KIFC1 Antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Observed band size:74 kDa.")
![Immunohistochemistry-Paraffin: KIFC1 Antibody [NBP3-06407] - Immunochemical staining of human KIFC1 in human tonsil with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/KIFC1-Antibody-Immunohistochemistry-Paraffin-NBP3-06407-img0003.jpg)
![Immunohistochemistry-Paraffin: KIFC1 Antibody [NBP3-06407] - Immunochemical staining of human KIFC1 in human lymph node with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/KIFC1-Antibody-Immunohistochemistry-Paraffin-NBP3-06407-img0002.jpg)
![Western Blot: NUP62 Antibody (JE55-09) [NBP3-32670] - Western blot analysis of NUP62 on different lysates with Rabbit anti-NUP62 antibody (NBP3-32670) at 1/1,000 dilution. Lane 1: Jurkat cell lysate Lane 2: A431 cell lysate Lane 3: HeLa cell lysate Lane 4: Raji cell lysate Lane 5: HepG2 cell lysate Lane 6: C2C12 cell lysate Lane 7: RAW264.7 cell lysate Lane 8: PC-12 cell lysate Lane 9: NIH/3T3 cell lysate Lane 10: Neuro-2a cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 53 kDa Observed band size: 61 kDa Exposure time: 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32670) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32670_rabbit-nup62-mab-je55-09-278202415242615.jpg "Western Blot: NUP62 Antibody (JE55-09) [NBP3-32670] - Western blot analysis of NUP62 on different lysates with Rabbit anti-NUP62 antibody (NBP3-32670) at 1/1,000 dilution. Lane 1: Jurkat cell lysate Lane 2: A431 cell lysate Lane 3: HeLa cell lysate Lane 4: Raji cell lysate Lane 5: HepG2 cell lysate Lane 6: C2C12 cell lysate Lane 7: RAW264.7 cell lysate Lane 8: PC-12 cell lysate Lane 9: NIH/3T3 cell lysate Lane 10: Neuro-2a cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 53 kDa Observed band size: 61 kDa Exposure time: 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32670) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: NUP62 Antibody (JE55-09) [NBP3-32670] - Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-NUP62 antibody (NBP3-32670) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32670) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32670_rabbit-nup62-mab-je55-09-298202414262684.jpg)
![Immunohistochemistry-Paraffin: RBM7 Antibody [NBP2-97836] - Immunochemical staining of human RBM7 in human stomach with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/RBM7-Antibody-Immunohistochemistry-Paraffin-NBP2-97836-img0003.jpg "Immunohistochemistry-Paraffin: RBM7 Antibody [NBP2-97836] - Immunochemical staining of human RBM7 in human stomach with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.")
![Immunohistochemistry-Paraffin: RBM7 Antibody [NBP2-97836] - Immunochemical staining of human RBM7 in human corpus uteri with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/RBM7-Antibody-Immunohistochemistry-Paraffin-NBP2-97836-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: RBM7 Antibody [NBP2-97836] - Immunofluorescence staining of RBM7 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-97836 antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.](http://images.novusbio.com/fullsize/nbp2-97836_rabbit-polyclonal-rbm7-antibody-23520251129399.png)
![Immunohistochemistry: Cytokeratin 5/6 Antibody (PSH0-67) [NBP3-32251] - Immunofluorescence analysis of paraffin-embedded human lung squamous cell carcinoma tissue labeling Cytokeratin 5/6 with Rabbit anti-Cytokeratin 5/6 antibody (NBP3-32251) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32251, green) at 1/1,000 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32251_rabbit-cytokeratin-5-6-mab-psh0-67-29820241492674.jpg "Immunohistochemistry: Cytokeratin 5/6 Antibody (PSH0-67) [NBP3-32251] - Immunofluorescence analysis of paraffin-embedded human lung squamous cell carcinoma tissue labeling Cytokeratin 5/6 with Rabbit anti-Cytokeratin 5/6 antibody (NBP3-32251) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32251, green) at 1/1,000 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).")
![Immunohistochemistry: Cytokeratin 5/6 Antibody (PSH0-67) [NBP3-32251] - Immunohistochemical analysis of paraffin-embedded human breast tissue with Rabbit anti-Cytokeratin 5/6 antibody (NBP3-32251) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32251) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32251_rabbit-cytokeratin-5-6-mab-psh0-67-29820241420915.jpg)
![Western Blot: TEAD3 Antibody [NBP2-85896] - WB Suggested Anti-TEAD3 Antibody Titration: 0.2-1 ug/ml. Positive Control: HepG2 cell lysateTEAD3 is supported by BioGPS gene expression data to be expressed in HepG2](http://images.novusbio.com/fullsize/TEAD3-Antibody-Western-Blot-NBP2-85896-img0002.jpg "Western Blot: TEAD3 Antibody [NBP2-85896] - WB Suggested Anti-TEAD3 Antibody Titration: 0.2-1 ug/ml. Positive Control: HepG2 cell lysateTEAD3 is supported by BioGPS gene expression data to be expressed in HepG2")
![Immunohistochemistry: TEAD3 Antibody [NBP2-85896] - Human Intestine](http://images.novusbio.com/fullsize/TEAD3-Antibody-Immunohistochemistry-NBP2-85896-img0001.jpg)
![Western Blot: TEAD3 Antibody [NBP2-85896] - Host: Rabbit. Target Name: TEAD3. Sample Tissue: Human A549 Whole Cell. Antibody Dilution: 1ug/ml](http://images.novusbio.com/fullsize/TEAD3-Antibody-Western-Blot-NBP2-85896-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: USP6 Antibody [NBP2-76350] - Analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/USP6-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-76350-img0002.jpg "Immunocytochemistry/Immunofluorescence: USP6 Antibody [NBP2-76350] - Analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).")
![Flow Cytometry: USP6 Antibody [NBP2-76350] - Analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation overnight (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/USP6-Antibody-Flow-Cytometry-NBP2-76350-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: USP6 Antibody [NBP2-76350] - Analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic/vesicle staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/USP6-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-76350-img0001.jpg)
![Western Blot: Cytokeratin 18 Antibody (A4B3-R) [NBP3-32245] - Western blot analysis of Cytokeratin 18 on different lysates with Mouse anti-Cytokeratin 18 antibody (NBP3-32245) at 1/1,000 dilution. Lane 1: SK-Br-3 cell lysate (20 ug/Lane) Lane 2: A549 cell lysate (20 ug/Lane) Lane 3: A431 cell lysate (20 ug/Lane) Lane 4: MCF7 cell lysate (20 ug/Lane) Lane 5: Mouse liver tissue lysate (40 ug/Lane) Lane 6: Rat liver tissue lysate (40 ug/Lane) Predicted band size: 48 kDa Observed band size: 40-50 kDa Exposure time: Lane 1-4: 11 seconds; Lane 5-6: 2 minutes 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32245) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32245_mouse-cytokeratin-18-mab-a4b3-r-278202415242634.jpg "Western Blot: Cytokeratin 18 Antibody (A4B3-R) [NBP3-32245] - Western blot analysis of Cytokeratin 18 on different lysates with Mouse anti-Cytokeratin 18 antibody (NBP3-32245) at 1/1,000 dilution. Lane 1: SK-Br-3 cell lysate (20 ug/Lane) Lane 2: A549 cell lysate (20 ug/Lane) Lane 3: A431 cell lysate (20 ug/Lane) Lane 4: MCF7 cell lysate (20 ug/Lane) Lane 5: Mouse liver tissue lysate (40 ug/Lane) Lane 6: Rat liver tissue lysate (40 ug/Lane) Predicted band size: 48 kDa Observed band size: 40-50 kDa Exposure time: Lane 1-4: 11 seconds; Lane 5-6: 2 minutes 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32245) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Cytokeratin 18 Antibody (A4B3-R) [NBP3-32245] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-Cytokeratin 18 antibody (NBP3-32245) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32245) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32245_mouse-cytokeratin-18-mab-a4b3-r-29820241433369.jpg)
![Western Blot: TEF1 Antibody (JE45-37) [NBP3-33004] - Western blot analysis of TEF1 on different lysates with Rabbit anti-TEF1 antibody (NBP3-33004) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: A549 cell lysate Lane 3: 293T cell lysate Lane 4: SiHa cell lysate Lane 5: C2C12 cell lysate Lane 6: L6 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 48 kDa Observed band size: 48/50 kDa Exposure time: 3 minutes; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33004) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33004_rabbit-tef1-mab-je45-37-288202412493481.jpg "Western Blot: TEF1 Antibody (JE45-37) [NBP3-33004] - Western blot analysis of TEF1 on different lysates with Rabbit anti-TEF1 antibody (NBP3-33004) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: A549 cell lysate Lane 3: 293T cell lysate Lane 4: SiHa cell lysate Lane 5: C2C12 cell lysate Lane 6: L6 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 48 kDa Observed band size: 48/50 kDa Exposure time: 3 minutes; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33004) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: TEAD1 Antibody (JE45-37) [NBP3-33004] - Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue with Rabbit anti-TEAD1 antibody (NBP3-33004) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33004) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33004_rabbit-tead1-mab-je45-37-298202413585589.jpg)
![Western Blot: ZC3H7A Antibody [NBP3-06301] - Anti-ZC3H7A rabbit polyclonal antibody at 1:500 dilutionLane A: Hela Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L) HRP at 110000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:110 kDa](http://images.novusbio.com/fullsize/ZC3H7A-Antibody-Western-Blot-NBP3-06301-img0002.jpg "Western Blot: ZC3H7A Antibody [NBP3-06301] - Anti-ZC3H7A rabbit polyclonal antibody at 1:500 dilutionLane A: Hela Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L) HRP at 110000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:110 kDa")
![Immunoprecipitation: ZC3H7A Antibody [NBP3-06301] - ZC3H7A was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell Lysate4 uL anti-ZC3H7A rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein AG.Primary antibody:Anti-ZC3H7A rabbit polyclonal antibody, at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L) HRP at 110000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 110 kDaObserved band size :110 kDa](http://images.novusbio.com/fullsize/ZC3H7A-Antibody-Immunoprecipitation-NBP3-06301-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: ZC3H7A Antibody [NBP3-06301] - Immunofluorescence staining of ZC3H7A in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with NBP3-06301 antibody (dilution ratio 1:200) at 4 degrees celsius overnight. Then cells were stained with the Alexa Fluor (TM) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.](http://images.novusbio.com/fullsize/nbp3-06301_rabbit-polyclonal-zc3h7a-antibody-235202521525027.png)
![Western Blot: MAP2 Antibody (MT-01) [NB500-415] - Fig. 1. Western Blotting analysis (reducing conditions) of microtubules partially purified from porcine brain lysate. Lane 1: immunostaining with anti-MAP2ab (MT-01) Lane 2: immunostaining with anti-MAP2ab (MT-07) Lane 3: immunostaining with anti-MAP2ab (MT-08). Observed molecular weight is approximately 270 kDA.](http://images.novusbio.com/fullsize/MAP2-Antibody-MT-01-Western-Blot-NB500-415-img0001.jpg "<b>Independent Antibodies Validation. </b>Western Blot: MAP2 Antibody (MT-01) [NB500-415] - Fig. 1. Western Blotting analysis (reducing conditions) of microtubules partially purified from porcine brain lysate. Lane 1: immunostaining with anti-MAP2ab (MT-01) Lane 2: immunostaining with anti-MAP2ab (MT-07) Lane 3: immunostaining with anti-MAP2ab (MT-08). Observed molecular weight is approximately 270 kDA.")
![Western Blot: ACTR3 Antibody [NBP2-97009] - Anti-ACTR3 rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate. Lane B: Jurkat Whole Cell Lysate. Lane C: A549 Whole Cell Lysate. Lane D: K562 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 47 kDa. Observed band size: 45 kDa (We are unsure as to the identity of these extra bands).](http://images.novusbio.com/fullsize/ACTR3-Antibody-Western-Blot-NBP2-97009-img0005.jpg "Western Blot: ACTR3 Antibody [NBP2-97009] - Anti-ACTR3 rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate. Lane B: Jurkat Whole Cell Lysate. Lane C: A549 Whole Cell Lysate. Lane D: K562 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 47 kDa. Observed band size: 45 kDa (We are unsure as to the identity of these extra bands).")
![Immunoprecipitation: ACTR3 Antibody [NBP2-97009] - ACTR3 was immunoprecipitated using: Lane A: 0.5 mg K562 Whole Cell Lysate. Lane B: 0.5 mg Hela Whole Cell Lysate. 4 ul anti-ACTR3 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-ACTR3 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Clean-Bloto IP Detection Reagent (HRP) at 1:500 dilution. Developed using the DAB staining technique. Performed under reducing conditions. Predicted band size: 47 kDa. Observed band size: 47 kDa](http://images.novusbio.com/fullsize/ACTR3-Antibody-Immunoprecipitation-NBP2-97009-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: ACTR3 Antibody [NBP2-97009] - Immunofluorescence staining of ACTR3 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-97009 antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.](http://images.novusbio.com/fullsize/nbp2-97009_rabbit-polyclonal-actr3-antibody-235202521525017.png)
![Western Blot: ELAC2 Antibody [NBP2-97772] - Anti-ELAC2 rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 92 kDa. Observed band size: 95 kDa](http://images.novusbio.com/fullsize/ELAC2-Antibody-Western-Blot-NBP2-97772-img0004.jpg "Western Blot: ELAC2 Antibody [NBP2-97772] - Anti-ELAC2 rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 92 kDa. Observed band size: 95 kDa")
![Immunoprecipitation: ELAC2 Antibody [NBP2-97772] - ELAC2 was immunoprecipitated using: Lane A: 0.5 mg Jurkat Whole Cell Lysate 4 ul anti-ELAC2 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-ELAC2 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 92 kDa. Observed band size: 95 kDa](http://images.novusbio.com/fullsize/ELAC2-Antibody-Immunoprecipitation-NBP2-97772-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: ELAC2 Antibody [NBP2-97772] - Immunofluorescence staining of ELAC2 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-97772 antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.](http://images.novusbio.com/fullsize/nbp2-97772_rabbit-polyclonal-elac2-antibody-235202521451778.png)