| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB500-318-25ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB500-212SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59800SS | |||
| Azide and BSA Free | NBP2-80830 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-54808SS | |||
| Azide and BSA Free | NBP2-80729 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56168SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-24389SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56096SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87702-25ul | |||
![Western Blot: LXR alpha/NR1H3 Antibody (JA20-38) [NBP2-66938] - Analysis of LXR alpha/NR1H3 on rat brain tissue lysates with Rabbit anti-LXR alpha antibody at 1/500 dilution. Lysates/proteins at 20 ug/Lane. Predicted band size: 50 kDa Observed band size: 50 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-JA20-38-Western-Blot-NBP2-66938-img0009.jpg "Western Blot: LXR alpha/NR1H3 Antibody (JA20-38) [NBP2-66938] - Analysis of LXR alpha/NR1H3 on rat brain tissue lysates with Rabbit anti-LXR alpha antibody at 1/500 dilution. Lysates/proteins at 20 ug/Lane. Predicted band size: 50 kDa Observed band size: 50 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: LXR alpha/NR1H3 Antibody (JA20-38) [NBP2-66938] - Staining LXR alpha/NR1H3 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-JA20-38-Immunocytochemistry-Immunofluorescence-NBP2-66938-img0002.jpg)
![Immunohistochemistry-Paraffin: LXR alpha/NR1H3 Antibody (JA20-38) [NBP2-66938] - Analysis of paraffin-embedded mouse testes tissue using anti-LXR alpha/NR1H3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-JA20-38-Immunohistochemistry-Paraffin-NBP2-66938-img0005.jpg)
![Flow Cytometry: LXR alpha/NR1H3 Antibody (JA20-38) [NBP2-66938] - Analysis of HepG2 cells with LXR alpha/NR1H3 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-JA20-38-Flow-Cytometry-NBP2-66938-img0001.jpg)
![Western Blot: LXR alpha/NR1H3 Antibody (JA20-38) [NBP2-66938] - Analysis of LXR alpha on mouse colon tissue lysate using anti-LXR alpha/NR1H3 antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-JA20-38-Western-Blot-NBP2-66938-img0006.jpg)
![Western Blot: LXR alpha/NR1H3 Antibody (JA20-38) [NBP2-66938] - Analysis of LXR alpha/NR1H3 on human liver tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-JA20-38-Western-Blot-NBP2-66938-img0007.jpg)
![Western Blot: LXR alpha/NR1H3 Antibody (JA20-38) [NBP2-66938] - Analysis of LXR alpha/NR1H3 on mouse colon tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-JA20-38-Western-Blot-NBP2-66938-img0008.jpg)
![Immunohistochemistry-Paraffin: LXR alpha/NR1H3 Antibody (JA20-38) [NBP2-66938] - Analysis of paraffin-embedded human placenta tissue using anti-LXR alpha/NR1H3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-JA20-38-Immunohistochemistry-Paraffin-NBP2-66938-img0003.jpg)
![Immunohistochemistry-Paraffin: LXR alpha/NR1H3 Antibody (JA20-38) [NBP2-66938] - Analysis of paraffin-embedded human spleen tissue using anti-LXR alpha/NR1H3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-JA20-38-Immunohistochemistry-Paraffin-NBP2-66938-img0004.jpg)
![Western Blot: TOMM20 Antibody (ST04-72) [NBP2-67501] - Analysis of TOMM20 on different lysates using anti-TOMM20 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: MCF-7 Lane 3: F9 Lane 4: PC12](http://images.novusbio.com/fullsize/TOMM20-Antibody-ST04-72-Western-Blot-NBP2-67501-img0010.jpg "Western Blot: TOMM20 Antibody (ST04-72) [NBP2-67501] - Analysis of TOMM20 on different lysates using anti-TOMM20 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: MCF-7 Lane 3: F9 Lane 4: PC12")
![Immunocytochemistry/Immunofluorescence: TOMM20 Antibody (ST04-72) [NBP2-67501] - Staining TOMM20 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/TOMM20-Antibody-ST04-72-Immunocytochemistry-Immunofluorescence-NBP2-67501-img0004.jpg)
![Immunohistochemistry-Paraffin: TOMM20 Antibody (ST04-72) [NBP2-67501] - Analysis of paraffin-embedded human liver tissue with Rabbit anti-TOMM20 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/TOMM20-Antibody-ST04-72-Immunohistochemistry-Paraffin-NBP2-67501-img0013.jpg)
![Flow Cytometry: TOMM20 Antibody (ST04-72) [NBP2-67501] - Analysis of Hela cells with TOMM20 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody](http://images.novusbio.com/fullsize/TOMM20-Antibody-ST04-72-Flow-Cytometry-NBP2-67501-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: TOMM20 Antibody (ST04-72) [NBP2-67501] - Staining TOMM20 in PC12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/TOMM20-Antibody-ST04-72-Immunocytochemistry-Immunofluorescence-NBP2-67501-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: TOMM20 Antibody (ST04-72) [NBP2-67501] - Staining TOMM20 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/TOMM20-Antibody-ST04-72-Immunocytochemistry-Immunofluorescence-NBP2-67501-img0003.jpg)
![Immunohistochemistry-Paraffin: TOMM20 Antibody (ST04-72) [NBP2-67501] - Analysis of paraffin-embedded human kidney tissue using anti-TOMM20 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/TOMM20-Antibody-ST04-72-Immunohistochemistry-Paraffin-NBP2-67501-img0005.jpg)
![Immunohistochemistry-Paraffin: TOMM20 Antibody (ST04-72) [NBP2-67501] - Analysis of paraffin-embedded human liver cancer tissue using anti-TOMM20 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/TOMM20-Antibody-ST04-72-Immunohistochemistry-Paraffin-NBP2-67501-img0006.jpg)
![Immunohistochemistry-Paraffin: TOMM20 Antibody (ST04-72) [NBP2-67501] - Analysis of paraffin-embedded mouse heart tissue using anti-TOMM20 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/TOMM20-Antibody-ST04-72-Immunohistochemistry-Paraffin-NBP2-67501-img0007.jpg)
![Immunohistochemistry-Paraffin: TOMM20 Antibody (ST04-72) [NBP2-67501] - Analysis of paraffin-embedded mouse kidney tissue using anti-TOMM20 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/TOMM20-Antibody-ST04-72-Immunohistochemistry-Paraffin-NBP2-67501-img0008.jpg)
![Immunohistochemistry-Paraffin: TOMM20 Antibody (ST04-72) [NBP2-67501] - Analysis of paraffin-embedded mouse small intestine tissue using anti-TOMM20 antibody. Counter stained with hematoxylin. The nuclear counter stain is DAPI (blue).](http://images.novusbio.com/fullsize/TOMM20-Antibody-ST04-72-Immunohistochemistry-Paraffin-NBP2-67501-img0009.jpg)
![Immunohistochemistry-Paraffin: TOMM20 Antibody (ST04-72) [NBP2-67501] - Analysis of paraffin-embedded human kidney tissue with Rabbit anti-TOMM20 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/TOMM20-Antibody-ST04-72-Immunohistochemistry-Paraffin-NBP2-67501-img0011.jpg)
![Immunohistochemistry-Paraffin: TOMM20 Antibody (ST04-72) [NBP2-67501] - Analysis of paraffin-embedded rat large intestine tissue with Rabbit anti-TOMM20 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/TOMM20-Antibody-ST04-72-Immunohistochemistry-Paraffin-NBP2-67501-img0012.jpg)
![Western blot: Rabbit Monoclonal TOMM20 Antibody (ST04-72) [NBP2-67501] - TOMM20 identification in different MSC cell lysates. No signal was obtained in extracellular vesicles. A secondary fluorescent antibody anti-rabbit was used. Image from a verified customer review.](http://images.novusbio.com/fullsize/nbp2-67501_rabbit-monoclonal-tomm20-antibody-st04-72-western-blot-69202313508..jpg)
![Western Blot: Rabbit Monoclonal TOMM20 Antibody (ST04-72) [NBP2-67501] - TOMM20 identification in rat astrocytes cell lysate. No signal was obtained in extracellular vesicles. A secondary fluorescent antibody anti-rabbit was used. The membrane was stripped and re-probed again. Image from a verified customer review.](http://images.novusbio.com/fullsize/nbp2-67501_rabbit-monoclonal-tomm20-antibody-st04-72-western-blot-2152025104540..jpg)
![Western Blot: ICAM-1/CD54 Antibody (MEM-111) [NB500-318] - ICAM-1 protein expression levels in rat lung tissue after the different treatments in each group. Immunoblotting showed increase in ICAM-1 after one course of paclitaxel compared with normal lung tissue; p < 0.01. These changes were reversed by using parecoxib in combination with paclitaxel, while the group that received parecoxib treatment alone resembled the Con group. The bar graph under the protein strap represents the corresponding band intensities; the data are presented as x +/- s and corrected by GA. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep12977), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-MEM-111-Western-Blot-NB500-318-img0006.jpg "Western Blot: ICAM-1/CD54 Antibody (MEM-111) [NB500-318] - ICAM-1 protein expression levels in rat lung tissue after the different treatments in each group. Immunoblotting showed increase in ICAM-1 after one course of paclitaxel compared with normal lung tissue; p < 0.01. These changes were reversed by using parecoxib in combination with paclitaxel, while the group that received parecoxib treatment alone resembled the Con group. The bar graph under the protein strap represents the corresponding band intensities; the data are presented as x +/- s and corrected by GA. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep12977), licensed under a CC-BY license.")
![Flow Cytometry: ICAM-1/CD54 Antibody (MEM-111) [NB500-318] - Flow cytometry analysis (surface staining) of CD54 expression in activated human peripheral blood with anti-CD54 (MEM-111) PE](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-MEM-111-Flow-Cytometry-NB500-318-img0005.jpg)
![Western Blot: ICAM-1/CD54 Antibody (MEM-111) [NB500-318] - Of CD54 expression in activated (A) and nonactivated (B) Huvec cells by antibody MEM-111. Lower bands represent tubulin as a loading control.](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-MEM-111-Western-Blot-NB500-318-img0001.jpg)
![Flow Cytometry: ICAM-1/CD54 Antibody (MEM-111) [NB500-318] - Analysis using the FITC conjugate of NB500-318. Staining of Human peripheral blood lymphocytes with MEM-111 FITC.](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-MEM-111-Flow-Cytometry-NB500-318-img0003.jpg)
![Flow Cytometry: ICAM-1/CD54 Antibody (MEM-111) [NB500-318] - Analysis using PE conjugate of NB500-318. A cell surface stain was performed on THP-1 cells with ICAM-1/CD54 antibody (MEM-111) NB500-318 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 1:100 for 20 minutes at room temperature. Both antibodies were conjugated to PE.](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-MEM-111-Flow-Cytometry-NB500-318-img0004.jpg)
![Western Blot: Villin 1 Antibody (JU34-75) [NBP2-75707] - Analysis of Villin1 on different tissue lysates using anti-Villin1 antibody at 1:500 dilution. Positive control:Lane 1: Mouse colon. Lane 2: Human small intestine. Lane 3: Human colon. Lane 4: Rat kidney.](http://images.novusbio.com/fullsize/Villin-1-Antibody-JU34-75-Western-Blot-NBP2-75707-img0008.jpg "Western Blot: Villin 1 Antibody (JU34-75) [NBP2-75707] - Analysis of Villin1 on different tissue lysates using anti-Villin1 antibody at 1:500 dilution. Positive control:Lane 1: Mouse colon. Lane 2: Human small intestine. Lane 3: Human colon. Lane 4: Rat kidney.")
![Immunocytochemistry/Immunofluorescence: Villin 1 Antibody (JU34-75) [NBP2-75707] - Staining Villin1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X-100/PBS.](http://images.novusbio.com/fullsize/Villin-1-Antibody-JU34-75-Immunocytochemistry-Immunofluorescence-NBP2-75707-img0004.jpg)
![Immunohistochemistry-Paraffin: Villin 1 Antibody (JU34-75) [NBP2-75707] - Analysis of paraffin-embedded mouse colon tissue using anti-Villin1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Villin-1-Antibody-JU34-75-Immunohistochemistry-Paraffin-NBP2-75707-img0007.jpg)
![Flow Cytometry: Villin 1 Antibody (JU34-75) [NBP2-75707] - Analysis of HeLa cells with Villin1 antibody at 1:100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/Villin-1-Antibody-JU34-75-Flow-Cytometry-NBP2-75707-img0001.jpg)
![Western Blot: Villin 1 Antibody (JU34-75) [NBP2-75707] - Caco-2 cell line lysate. WB image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Villin-1-Antibody-JU34-75-Western-Blot-NBP2-75707-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: Villin 1 Antibody (JU34-75) [NBP2-75707] - Staining Villin1 in HeLa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X-100/PBS.](http://images.novusbio.com/fullsize/Villin-1-Antibody-JU34-75-Immunocytochemistry-Immunofluorescence-NBP2-75707-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Villin 1 Antibody (JU34-75) [NBP2-75707] - Staining Villin1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X-100/PBS.](http://images.novusbio.com/fullsize/Villin-1-Antibody-JU34-75-Immunocytochemistry-Immunofluorescence-NBP2-75707-img0003.jpg)
![Immunohistochemistry-Paraffin: Villin 1 Antibody (JU34-75) [NBP2-75707] - Analysis of paraffin-embedded human colon cancer tissue using anti-Villin1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Villin-1-Antibody-JU34-75-Immunohistochemistry-Paraffin-NBP2-75707-img0005.jpg)
![Immunohistochemistry-Paraffin: Villin 1 Antibody (JU34-75) [NBP2-75707] - Analysis of paraffin-embedded human kidney tissue using anti-Villin1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Villin-1-Antibody-JU34-75-Immunohistochemistry-Paraffin-NBP2-75707-img0006.jpg)
![Beclin 1 Antibody Pack [NB910-95610] - Lane view shows a specific band for Beclin in 1.0 mg/ml of HeLa lysate using NB110-87318. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Beclin-1-Antibody-Pack-NB910-95610-img0014.jpg "Beclin 1 Antibody Pack [NB910-95610] - Lane view shows a specific band for Beclin in 1.0 mg/ml of HeLa lysate using NB110-87318. This experiment was performed under reducing conditions using the 12-230 kDa separation system.")
![<b>Orthogonal Strategies Validation. </b>Beclin 1 Antibody Pack [NB910-95610] - Expression of Beclin 1 mRNA and protein in cells and tissues. (A) Beclin 1 mRNA expression level up-regulated in gastric cancer cell lines compared with normal gastric epithelial cells by reverse transcription-PCR. (B) Beclin 1 protein expression level up-regulated in gastric cancer cell lines compared with normal gastric epithelial cells by western blotting. (C) Beclin 1 mRNA expression is elevated in primary gastric tumors compared with paired gastric adjacent noncancerous tissues by reverse transcription-PCR. (D) Beclin 1 protein expression is elevated in primary gastric tumors compared with paired gastric adjacent noncancerous tissues by western blotting. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0045968) licensed under a CC-BY license. Anti-Beclin-1 Antibody NB500-249](http://images.novusbio.com/fullsize/Beclin-1-Antibody-Pack-NB910-95610-img0013.jpg)
![Immunohistochemistry: Beclin 1 Antibody Pack [NB910-95610] - Analysis of a FFPE tissue section of human breast cancer using Beclin 1 antibody (NB110-87318) at 1:300 dilution. The primary antibody bound to Beclin 1 antigen in the tissue section was detected using a HRP labeled secondary antibody and DAB reagent. Nuclei of the cells were counterstained with hematoxylin. Cytoplasmic staining can be seen in the cancer as well as the stromal cells. The signal was strongest in a subset of stromal cells which appears to be the cancer associated fibroblasts.](http://images.novusbio.com/fullsize/Beclin-1-Antibody-Pack-Immunohistochemistry-NB910-95610-img0018.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Beclin 1 Antibody Pack [NB910-95610] - siRNA knockdown of becn1 (Beclin 1). Lysates from OKP7 cells treated with non-targeting siRNA (non-target) or an siRNA pool directed against Beclin 1 (becn1) were analyzed by Western blot for Beclin 1 production using NB500-249. GAPDH was used as a loading control. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.ppat.1003394) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Beclin-1-Antibody-Pack-Knockdown-Validated-NB910-95610-img0016.jpg)
![Immunocytochemistry/Immunofluorescence: Beclin 1 Antibody Pack [NB910-95610] - Beclin 1 antibody (NB500-249) was tested in HeLa cells with Dylight 488 (green, left panel). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red), shown in the overlay (right panel)](http://images.novusbio.com/fullsize/Beclin-1-Antibody-Pack-Immunocytochemistry-Immunofluorescence-NB910-95610-img0020.jpg)
![Immunohistochemistry: Beclin 1 Antibody Pack [NB910-95610] - Detecting Beclin 1 using NB500-249 in Pheochromocytes of the Adrenal Medulla at 40x.](http://images.novusbio.com/fullsize/Beclin-1-Antibody-Pack-Immunohistochemistry-NB910-95610-img0011.jpg)
![Western Blot: Beclin 1 Antibody Pack [NB910-95610] - Analysis of Beclin1 using NB500-249. Lane 1: human brain. Lane 2: mouse brain.](http://images.novusbio.com/fullsize/Beclin-1-Antibody-Pack-Western-Blot-NB910-95610-img0015.jpg)
![Western Blot: Beclin 1 Antibody Pack [NB910-95610] - Detection of Beclin 1 in liver lysates using NB 500-266. Lane 1: mouse liver Lane 2: human liver.](http://images.novusbio.com/fullsize/Beclin-1-Antibody-Pack-Western-Blot-NB910-95610-img0012.jpg)
![Western Blot: CHAF1B Antibody (SS 53) [NB500-212] - A. RKO cells with CAF-1 p150, CAF-1 p60 and PCNA antibodies. B. p150 RNAi knockdown. Reference Pubmed 14519857.](http://images.novusbio.com/fullsize/CHAF1B-Antibody-SS-53-Western-Blot-NB500-212-img0002.jpg "<b>Genetic Strategies Validation. </b>Western Blot: CHAF1B Antibody (SS 53) [NB500-212] - A. RKO cells with CAF-1 p150, CAF-1 p60 and PCNA antibodies. B. p150 RNAi knockdown. Reference Pubmed 14519857.")
![Western Blot: LC3B Antibody (1251D) [NBP2-59800] - HeLa human cervical epithelial carcinoma parental cell line and LC3B knockout HeLa cell line (KO) untreated (-) or treated (+) with 50 uM Chloroquine for 18 hrs. PVDF membrane was probed with 0.1 ug/mL of Rabbit Anti-LC3B Monoclonal Antibody (1251D) [Catalog# NBP2-59800] followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog# HAF008). A specific band was detected for LC3B at approximately 15 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/LC3B-Antibody-1251D-Knockout-Validated-NBP2-59800-img0005.jpg "<b>Genetic Strategies Validation. </b>Western Blot: LC3B Antibody (1251D) [NBP2-59800] - HeLa human cervical epithelial carcinoma parental cell line and LC3B knockout HeLa cell line (KO) untreated (-) or treated (+) with 50 uM Chloroquine for 18 hrs. PVDF membrane was probed with 0.1 ug/mL of Rabbit Anti-LC3B Monoclonal Antibody (1251D) [Catalog# NBP2-59800] followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog# HAF008). A specific band was detected for LC3B at approximately 15 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions.")
![<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Western Blot: LC3B Antibody (1251D) [NBP2-59800] - Total protein from HeLa and Neuro2A cells treated with or without 50 uM chloroquine for 24 hrs was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF (Polyvinylidene difluoride) membrane and blocked in 5% non-fat milk in TBST (Tris-buffered saline, 0.1% Tween 20). The membrane was probed with 2.0 ug/ml anti-LC3B Antibody (1251d) [Catalog # NBP2-59800] in blocking buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. Note the detection of LC3I and LC3II at a molecular weight of approximately 15 kDa.](http://images.novusbio.com/fullsize/LC3B-Antibody-1251D-Western-Blot-NBP2-59800-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: LC3B Antibody (1251D) [NBP2-59800] - HeLa cells were treated with 50uM CQ (Chloroquine) overnight, fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-LC3B (1251d) [Catalog # NBP2-59800] at 5 ug/ml overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/LC3B-Antibody-1251D-Immunocytochemistry-Immunofluorescence-NBP2-59800-img0003.jpg)
![Immunohistochemistry-Paraffin: LC3B Antibody (1251D) [NBP2-59800] - Analysis of a FFPE (Formalin-Fixed Paraffin-Embedded) tissue section of mouse brain using anti-LC3B antibody (clone 1251D) [Catalog # NBP2-59800] at 5ug/ml concentration (1:200 dilution). The primary antibody binding to LC3 in cells was detected using HRP conjugated anti-Rabbit secondary antibody with DAB (3,3'Diaminobenzidine) reagent, and the sections were further counterstained with hematoxylin for labeling cellular nuclei. This LC3 antibody generated a diffused staining in all cell types (except the endothelial cells of blood vessels) and the signal was strongest in a subset of neuronal cells.](http://images.novusbio.com/fullsize/LC3B-Antibody-1251D-Immunohistochemistry-Paraffin-NBP2-59800-img0023.jpg)
![<b>Biological Strategies Validation. </b>Flow (Intracellular): LC3B Antibody (1251D) [NBP2-59800] - HeLa cells were either (A) untreated or (B) treated with 50uM chloroquine for 24 hrs. An intracellular stain was performed with anti-LC3B (1251D) antibody [Catalog # NBP2-59800] (blue) and a matched isotype control [Catalog # MAB1050] (orange). Cells were fixed with 4% paraformaldehyde, following fixation, cells were permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by rabbit IgG APC-conjugated secondary antibody (F0111, R&D Systems).](http://images.novusbio.com/fullsize/LC3B-Antibody-1251D-Flow-Intracellular-NBP2-59800-img0022.jpg)
![<b>Biological Strategies Validation. </b>Simple Western: LC3B Antibody (1251D) [NBP2-59800] - Lane view shows lysates of NIH-3T3 mouse embryonic fibroblast cell line untreated (-) or treated (+) with 50 uM Chloroquine for 18 hrs, loaded at 0.2 mg/ml. A specific band was detected for LC3B at approximately 17 kDa (as indicated) using 5 ug/mL of Rabbit Anti-Human LC3B Monoclonal Antibody (1251D) [Catalog# NBP2-59800]. This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/LC3B-Antibody-1251D-Simple-Western-NBP2-59800-img0021.jpg)
![Western Blot: FANCD2 Antibody (1290D) [NBP2-54808] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and FANCD2 knockout (KO) HeLa cell line. PVDF membrane was probed with 0.1 ug/mL of Rabbit Monoclonal (Catalog # NBP2-54808) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for FANCD2 at approximately 150 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/FANCD2-Antibody-1290D-Knockout-Validated-NBP2-54808-img0003.jpg "<b>Genetic Strategies Validation. </b>Western Blot: FANCD2 Antibody (1290D) [NBP2-54808] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and FANCD2 knockout (KO) HeLa cell line. PVDF membrane was probed with 0.1 ug/mL of Rabbit Monoclonal (Catalog # NBP2-54808) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for FANCD2 at approximately 150 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Simple Western: FANCD2 Antibody (1290D) [NBP2-54808] - Detection of Human FANCD2 by Simple Western using FANCD2 Antibody (1290D) in HeLa and K562 cell lysate.](http://images.novusbio.com/fullsize/FANCD2-Antibody-1290D-Simple-Western-NBP2-54808-img0002.jpg)
![Immunohistochemistry: FANCD2 Antibody (1290D) [NBP2-54808] - FANCD2 was detected in immersion fixed paraffin-embedded sections of human tonsil using Rabbit Anti-Human FANCD2 Antibody (1290D) (Catalog # NBP2-54808) at 5 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte(TM) HRP Polymer Antibody (Catalog # VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei.](http://images.novusbio.com/fullsize/FANCD2-Antibody-1290D-Immunohistochemistry-NBP2-54808-img0004.jpg)
![Flow (Intracellular): FANCD2 Antibody (1290D) [NBP2-54808] - An intracellular stain was performed on HeLa Cells with FANCD2 Antibody (1290D) (blue) and a matched isotype control MAB1050 (orange). Cells were fixed with 4% paraformaldehyde, following fixation, cells were permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by rabbit IgG APC-conjugated secondary antibody (F0111, R&D Systems).](http://images.novusbio.com/fullsize/FANCD2-Antibody-1290D-Flow-Intracellular-NBP2-54808-img0005.jpg)
![Western Blot: FANCD2 Antibody (1290D) [NBP2-54808] - Total protein from HeLa and K562 cells (Molecular weight: 164.1 KDa) was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 0.1 ug/ml FANCD2 Antibody (1290D) (catalog number NBP2-54808) in blocking buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/FANCD2-Antibody-1290D-Western-Blot-NBP2-54808-img0006.jpg)
![Western Blot: Survivin Antibody [NB100-56168] - Analysis of Normal Prostate Epithelium (NPE) and Prostate Cancer using [NB100-56168]. 100 ug/lane of each human tissue lysate was loaded per lane. Survivin was detected in 7 out of 8 prostate cancer patient specimens, but was not detected in NPE. Bands detected at predicted molecular weight of 16 kDa.](http://images.novusbio.com/fullsize/Survivin-Antibody-Western-Blot-NB100-56168-img0005.jpg "Western Blot: Survivin Antibody [NB100-56168] - Analysis of Normal Prostate Epithelium (NPE) and Prostate Cancer using [NB100-56168]. 100 ug/lane of each human tissue lysate was loaded per lane. Survivin was detected in 7 out of 8 prostate cancer patient specimens, but was not detected in NPE. Bands detected at predicted molecular weight of 16 kDa.")
![Immunohistochemistry-Paraffin: Survivin Antibody [NB100-56168] - Analysis of Human Esophageal Cancer at 1:2000 using Survivin Antibody [NB100-56168]. A1 is a high magnification of A. Hematoxylin-eosin counterstain.](http://images.novusbio.com/fullsize/Survivin-Antibody-Immunohistochemistry-Paraffin-NB100-56168-img0002.jpg)
![Immunohistochemistry-Paraffin: Survivin Antibody [NB100-56168] - Analysis of Human Prostate Cancer array at 1:2000 using Survivin Antibody [NB100-56168]. A1 is a high magnification of A. Differential expression of Survivin is seen in tissue cores from four different prostate cancer patients. Hematoxylin-eosin counterstain.](http://images.novusbio.com/fullsize/Survivin-Antibody-Immunohistochemistry-Paraffin-NB100-56168-img0004.jpg)
![Western Blot: ATG5 Antibody [NBP2-24389] - Western blot analysis of human APGL5 in T98G cell lysate in 1) absence, 2) presence of immunizing peptide using this antibody at 1 ug/mL.](http://images.novusbio.com/fullsize/ATG5-Antibody-Western-Blot-NBP2-24389-img0001.jpg "Western Blot: ATG5 Antibody [NBP2-24389] - Western blot analysis of human APGL5 in T98G cell lysate in 1) absence, 2) presence of immunizing peptide using this antibody at 1 ug/mL.")
![Immunohistochemistry-Paraffin: ATG5 Antibody [NBP2-24389] - Immunohistochemistry analysis of human kidney using this antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/ATG5-Antibody-Immunohistochemistry-Paraffin-NBP2-24389-img0002.jpg)
![Western Blot: Bax Antibody [NB100-56096] - Analysis of Bax in mouse using this antibody. 1. In vitro translated mouse Bax. 2. Normal cortex. 3. Ischemic hippocampus. 4. Ischemic hippocampus. Ishemic samples were prepared 6 hr post middle cerebral artery occlusion. The results show the Bax expression increased following ischemia.](http://images.novusbio.com/fullsize/Bax-Antibody-Western-Blot-NB100-56096-img0003.jpg "Western Blot: Bax Antibody [NB100-56096] - Analysis of Bax in mouse using this antibody. 1. In vitro translated mouse Bax. 2. Normal cortex. 3. Ischemic hippocampus. 4. Ischemic hippocampus. Ishemic samples were prepared 6 hr post middle cerebral artery occlusion. The results show the Bax expression increased following ischemia.")
![Immunohistochemistry-Paraffin: Bax Antibody [NB100-56096] - Mouse tissue sections stained for Bax using NB100-56096 at 1:2000. A. Pancreas. B. Brain showing expression of Bax in the Purkinge cells.](http://images.novusbio.com/fullsize/Bax-Antibody-Immunohistochemistry-Paraffin-NB100-56096-img0002.jpg)
![Immunohistochemistry-Paraffin: Indoleamine 2,3-dioxygenase/IDO Antibody [NBP1-87702] - Staining in human placenta and cerebral cortex tissues . Corresponding IDO1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Indoleamine-2-3-dioxygenase-IDO-Antibody-Immunohistochemistry-Paraffin-NBP1-87702-img0016.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Indoleamine 2,3-dioxygenase/IDO Antibody [NBP1-87702] - Staining in human placenta and cerebral cortex tissues . Corresponding IDO1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: Indoleamine 2,3-dioxygenase/IDO Antibody [NBP1-87702] - Analysis in human cell line EFO-21.](http://images.novusbio.com/fullsize/Indoleamine-2-3-dioxygenase-IDO-Antibody-Western-Blot-NBP1-87702-img0013.jpg)
![Immunohistochemistry-Paraffin: Indoleamine 2,3-dioxygenase/IDO Antibody [NBP1-87702] - Staining of human lymph node shows strong cytoplasmic and nuclear positivity in non-germinal center cells.](http://images.novusbio.com/fullsize/Indoleamine-2-3-dioxygenase-IDO-Antibody-Immunohistochemistry-Paraffin-NBP1-87702-img0018.jpg)
![Immunohistochemistry-Paraffin: Indoleamine 2,3-dioxygenase/IDO Antibody [NBP1-87702] - Immunohistochemical staining of human placenta shows strong cytoplasmic and nuclear positivity in endothelial cells.](http://images.novusbio.com/fullsize/Indoleamine-2-3-dioxygenase-IDO-Antibody-Immunohistochemistry-Paraffin-NBP1-87702-img0009.jpg)
![Immunohistochemistry-Paraffin: Indoleamine 2,3-dioxygenase/IDO Antibody [NBP1-87702] - Immunohistochemical staining of human cerebral cortex shows no positivity as expected.](http://images.novusbio.com/fullsize/Indoleamine-2-3-dioxygenase-IDO-Antibody-Immunohistochemistry-Paraffin-NBP1-87702-img0010.jpg)
![Immunohistochemistry-Paraffin: Indoleamine 2,3-dioxygenase/IDO Antibody [NBP1-87702] - Immunohistochemical staining of human small intestine shows strong cytoplasmic and nuclear positivity in a subset of leukocytes.](http://images.novusbio.com/fullsize/Indoleamine-2-3-dioxygenase-IDO-Antibody-Immunohistochemistry-Paraffin-NBP1-87702-img0011.jpg)
![Simple Western: Indoleamine 2,3-dioxygenase/IDO Antibody [NBP1-87702] - Simple Western lane view shows a specific band for IDO1 in 0.2 mg/ml of OVCAR-3 (left), h. Tonsil, raji, daudi lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Indoleamine-2-3-dioxygenase-IDO-Antibody-Simple-Western-NBP1-87702-img0005.jpg)
![Simple Western: Indoleamine 2,3-dioxygenase/IDO Antibody [NBP1-87702] - Electropherogram image(s) of corresponding Simple Western lane view. Indoleamine 2,3-dioxygenase/IDO antibody was used at 1:20 dilution on OVCAR-3, h. Tonsil, Raji, and Daudi lysate(s).](http://images.novusbio.com/fullsize/Indoleamine-2-3-dioxygenase-IDO-Antibody-Simple-Western-NBP1-87702-img0008.jpg)