| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-03767-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-92954-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-94856-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-03522-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-03406-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35830-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-15485-20ul | |||
![Western Blot: ELF3/ESE-1 Antibody (7O2V4) [NBP3-16429] - Western blot analysis of extracts of various cell lines, using ELF3/ESE-1 Rabbit mAb (NBP3-16429) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/ELF3-ESE-1-Antibody-7O2V4-Western-Blot-NBP3-16429-img0001.jpg "Western Blot: ELF3/ESE-1 Antibody (7O2V4) [NBP3-16429] - Western blot analysis of extracts of various cell lines, using ELF3/ESE-1 Rabbit mAb (NBP3-16429) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Chromatin Immunoprecipitation: ELF3/ESE-1 Antibody (7O2V4) [NBP3-16429] - Analysis of extracts of HepG2 cells, using ESE1/ELF3 antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.](http://images.novusbio.com/fullsize/nbp3-16429_rabbit-elf3-ese-1-mab-7o2v4-chromatin-immunoprecipitation-chip--2492024124532..jpg)
![Chip IP Sequencing: ELF3/ESE-1 Antibody (7O2V4) [NBP3-16429] -CUT&Tag was performed using the CUT&Tag Assay Kit (pAG-Tn5) for Illumina from 10⁵ HepG5 with 1μg ESE1 along with a Goat Anti-Rabbit IgG(H+L). The CUT&Tag results indicate the enrichment pattern of ESE1 in representative gene loci (MARK4), as shown in figure.](http://images.novusbio.com/fullsize/nbp3-16429_rabbit-elf3-ese-1-mab-7o2v4-chromatin-immunoprecipitation-sequencing-2492024131643..jpg)
![Chromatin Immunoprecipitation: ELF3/ESE-1 Antibody (7O2V4) [NBP3-16429] - Chromatin immunoprecipitation analysis of extracts of HepG2 cells, using ELF3/ESE-1 antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.](http://images.novusbio.com/fullsize/nbp3-16429_rabbit-elf3-ese-1-mab-7o2v4-52202511374128.jpg)
![Chromatin Immunoprecipitation: ELF3/ESE-1 Antibody (7O2V4) [ELF3/ESE-1] - Chromatin immunoprecipitation analysis of extracts of HepG2 cells, using ELF3/ESE-1 antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.](http://images.novusbio.com/fullsize/nbp3-16429_rabbit-elf3-ese-1-mab-7o2v4-52202517213584.jpg)
![Western Blot: ATG4C Antibody [NBP2-81954] - Analysis of ATG4C in A549 cell lysate with ATG4C antibody at 1 ug/ml.](http://images.novusbio.com/fullsize/ATG4C-Antibody-Western-Blot-NBP2-81954-img0001.jpg "Western Blot: ATG4C Antibody [NBP2-81954] - Analysis of ATG4C in A549 cell lysate with ATG4C antibody at 1 ug/ml.")
![Immunohistochemistry: ATG4C Antibody [NBP2-81954] - Analysis of ATG4C in human lung tissue with ATG4C antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/ATG4C-Antibody-Immunohistochemistry-NBP2-81954-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: ATG4C Antibody - BSA Free [NBP2-81954] - Immunofluorescence of ATG4C in human lung tissue with ATG4C antibody at 20 ug/ml.](http://images.novusbio.com/fullsize/nbp2-81954_rabbit-polyclonal-atg4c-antibody-271120242041457.jpg)
![Western Blot: FAM48A Antibody [NBP3-03767] - Analysis of extracts of various cell lines, using FAM48A antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit](http://images.novusbio.com/fullsize/FAM48A-Antibody-Western-Blot-NBP3-03767-img0005.jpg "Western Blot: FAM48A Antibody [NBP3-03767] - Analysis of extracts of various cell lines, using FAM48A antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit")
![Immunohistochemistry-Paraffin: FAM48A Antibody [NBP3-03767] - Paraffin-embedded mouse liver using FAM48A antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/FAM48A-Antibody-Immunohistochemistry-Paraffin-NBP3-03767-img0004.jpg)
![Immunohistochemistry-Paraffin: FAM48A Antibody [NBP3-03767] - Paraffin-embedded human colon using FAM48A antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/FAM48A-Antibody-Immunohistochemistry-Paraffin-NBP3-03767-img0002.jpg)
![Immunohistochemistry-Paraffin: FAM48A Antibody [NBP3-03767] - Paraffin-embedded human kidney using FAM48A antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/FAM48A-Antibody-Immunohistochemistry-Paraffin-NBP3-03767-img0003.jpg)
![Western Blot: AK2 Antibody [NBP2-92954] - Analysis of extracts of various cell lines, using AK2 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.](http://images.novusbio.com/fullsize/AK2-Antibody-Western-Blot-NBP2-92954-img0003.jpg "Western Blot: AK2 Antibody [NBP2-92954] - Analysis of extracts of various cell lines, using AK2 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.")
![Immunohistochemistry-Paraffin: AK2 Antibody [NBP2-92954] - Paraffin-embedded human gastric cancer using AK2 antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/AK2-Antibody-Immunohistochemistry-Paraffin-NBP2-92954-img0004.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: AK2 Antibody [NBP2-92954] - Analysis of extracts from normal (control) and AK2 knockout (KO) 293T cells, using AK2 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:Basic ECL Kit](http://images.novusbio.com/fullsize/AK2-Antibody-Knockout-Validated-NBP2-92954-img0005.jpg)
![Immunohistochemistry-Paraffin: SLC39A4/ZIP4 Antibody [NBP2-94856] - Rat liver using SLC39A4 antibody t dilution of 1:100 (40x lens). .](http://images.novusbio.com/fullsize/SLC39A4-ZIP4-Antibody-Immunohistochemistry-Paraffin-NBP2-94856-img0003.jpg "Immunohistochemistry-Paraffin: SLC39A4/ZIP4 Antibody [NBP2-94856] - Rat liver using SLC39A4 antibody t dilution of 1:100 (40x lens). .")
![Immunohistochemistry-Paraffin: SLC39A4/ZIP4 Antibody [NBP2-94856] - Human colon carcinoma using SLC39A4 antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/SLC39A4-ZIP4-Antibody-Immunohistochemistry-Paraffin-NBP2-94856-img0001.jpg)
![Immunohistochemistry-Paraffin: SLC39A4/ZIP4 Antibody [NBP2-94856] - Mouse lung using SLC39A4 antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/SLC39A4-ZIP4-Antibody-Immunohistochemistry-Paraffin-NBP2-94856-img0002.jpg)
![Western Blot: SLC39A4/ZIP4 Antibody - BSA Free [SLC39A4/ZIP4] - Western blot analysis of lysates from wild type (WT) and 293T cells transfected with SLC39A4/ZIP4 using SLC39A4/ZIP4 Rabbit pAb at 1:2000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp2-94856_rabbit-polyclonal-slc39a4-zip4-antibody-5220251638033.jpg)
![Western Blot: GAPDH-2 Antibody [NBP3-03522] - Analysis of extracts of various cell lines, using GAPDH-2 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.](http://images.novusbio.com/fullsize/GAPDH-2-Antibody-Western-Blot-NBP3-03522-img0001.jpg "Western Blot: GAPDH-2 Antibody [NBP3-03522] - Analysis of extracts of various cell lines, using GAPDH-2 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.")
![Immunohistochemistry: GAPDH-2 Antibody - BSA Free [GAPDH-2] - Immunohistochemistry analysis of paraffin-embedded Rat heart using GAPDH-2 Rabbit pAb at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol](http://images.novusbio.com/fullsize/nbp3-03522_rabbit-polyclonal-gapdh-2-antibody-52202516334192.jpg)
![Immunohistochemistry: GAPDH-2 Antibody - BSA Free [GAPDH-2] - Immunohistochemistry analysis of paraffin-embedded human breast using GAPDH-2 Rabbit pAb at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol](http://images.novusbio.com/fullsize/nbp3-03522_rabbit-polyclonal-gapdh-2-antibody-52202516293948.jpg)
![Immunohistochemistry: GAPDH-2 Antibody - BSA Free [GAPDH-2] - Immunohistochemistry analysis of paraffin-embedded Mouse testis using GAPDH-2 Rabbit pAb at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol](http://images.novusbio.com/fullsize/nbp3-03522_rabbit-polyclonal-gapdh-2-antibody-5220251628067.jpg)
![Immunohistochemistry-Paraffin: Nicotinamide N-Methyltransferase/NNMT Antibody [NBP3-03406] - Immunohistochemistry of paraffin-embedded human liver using [KO Validated] Nicotinamide N-Methyltransferase/NNMT Rabbit pAb (NBP3-03406) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Nicotinamide-N-Methyltransferase-NNMT-Antibody-Immunohistochemistry-Paraffin-NBP3-03406-img0003.jpg "Immunohistochemistry-Paraffin: Nicotinamide N-Methyltransferase/NNMT Antibody [NBP3-03406] - Immunohistochemistry of paraffin-embedded human liver using [KO Validated] Nicotinamide N-Methyltransferase/NNMT Rabbit pAb (NBP3-03406) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.")
![Western Blot: Nicotinamide N-Methyltransferase/NNMT Antibody [NBP3-03406] -analysis of A-549, using NNMT antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 60s.](http://images.novusbio.com/fullsize/antibody/nbp3-03406_rabbit-polyclonal-nicotinamide-n-methyltransferase-nnmt-antibody-western-blot-273202410843..jpg)
![Immunocytochemistry/ Immunofluorescence: Nicotinamide N-Methyltransferase/NNMT Antibody - Azide and BSA Free [Nicotinamide N-Methyltransferase/NNMT] - Immunofluorescence analysis of paraffin-embedded human liver cancer using Nicotinamide N-Methyltransferase/NNMT Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-03406_rabbit-polyclonal-nicotinamide-n-methyltransferase-nnmt-antibody-5220251628013.jpg)
![Immunohistochemistry: GPR109B/HM74 Antibody [NBP3-35830] - Immunohistochemistry analysis of paraffin-embedded Mouse spleen using GPR109B/HM74 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35830_rabbit-gpr109b-hm74-pab-101220241224170.jpg "Immunohistochemistry: GPR109B/HM74 Antibody [NBP3-35830] - Immunohistochemistry analysis of paraffin-embedded Mouse spleen using GPR109B/HM74 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.")
![Immunohistochemistry: GPR109B/HM74 Antibody [NBP3-35830] - Immunohistochemistry analysis of paraffin-embedded Rat spleen using GPR109B/HM74 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35830_rabbit-gpr109b-hm74-pab-1012202411592871.jpg)
![Western Blot: GPR109B/HM74 Antibody [NBP3-35830] - Western blot analysis of lysates from Rat pancreas, using GPR109B/HM74 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 120s.](http://images.novusbio.com/fullsize/nbp3-35830_rabbit-gpr109b-hm74-pab-1012202411535883.jpg)
![Immunohistochemistry: GPR109B/HM74 Antibody [NBP3-35830] - Immunohistochemistry analysis of paraffin-embedded Human spleen using GPR109B/HM74 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35830_rabbit-gpr109b-hm74-pab-1012202411531027.jpg)
![Western Blot: SIPA1L3 Antibody [NBP2-81902] - Analysis of SIPA1L3 in SK-N-SH lysate with SIPA1L3 antibody at 1 ug/ml.](http://images.novusbio.com/fullsize/SIPA1L3-Antibody-Western-Blot-NBP2-81902-img0001.jpg "Western Blot: SIPA1L3 Antibody [NBP2-81902] - Analysis of SIPA1L3 in SK-N-SH lysate with SIPA1L3 antibody at 1 ug/ml.")
![Immunocytochemistry/ Immunofluorescence: SIPA1L3 Antibody - BSA Free [NBP2-81902] - Immunofluorescence of SIPA1L3 in Human Brain cells with SIPA1L3 antibody at 20 u/mL.](http://images.novusbio.com/fullsize/nbp2-81902_rabbit-polyclonal-sipa1l3-antibody-271120241923626.jpg)
![Immunohistochemistry: SIPA1L3 Antibody - BSA Free [NBP2-81902] - Immunohistochemistry of SIPA1L3 in human brain tissue with SIPA1L3 antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/nbp2-81902_rabbit-polyclonal-sipa1l3-antibody-2711202419241332.jpg)
![Western Blot: HSP90 alpha Antibody (0R3S6) [NBP3-16528] - Western blot analysis of extracts of various cell lines, using HSP90 alpha Rabbit mAb (NBP3-16528) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.](http://images.novusbio.com/fullsize/HSP90-alpha-Antibody-0R3S6-Western-Blot-NBP3-16528-img0001.jpg "Western Blot: HSP90 alpha Antibody (0R3S6) [NBP3-16528] - Western blot analysis of extracts of various cell lines, using HSP90 alpha Rabbit mAb (NBP3-16528) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.")
![Immunoprecipitation: HSP90 alpha Antibody (0R3S6) [NBP3-16528] - Immunoprecipitation analysis of 300ug extracts of 293T cells using 3ug HSP90 alpha antibody (NBP3-16528). Western blot was performed from the immunoprecipitate using HSP90 alpha antibody (NBP3-16528) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/HSP90-alpha-Antibody-0R3S6-Immunoprecipitation-NBP3-16528-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: HSP90 alpha Antibody (0R3S6) [NBP3-16528] - Confocal imaging of paraffin-embedded Rat brain tissue using HSP90 alpha Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Microwave antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.](http://images.novusbio.com/fullsize/nbp3-16528_rabbit-hsp90-alpha-mab-0r3s6-52202511411441.jpg)
![Immunocytochemistry/ Immunofluorescence: HSP90 alpha Antibody (0R3S6) [NBP3-16528] - Confocal imaging of paraffin-embedded Mouse brain tissue using HSP90 alpha Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Microwave antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.](http://images.novusbio.com/fullsize/nbp3-16528_rabbit-hsp90-alpha-mab-0r3s6-52202511402778.jpg)
![Immunocytochemistry/ Immunofluorescence: HSP90 alpha Antibody (0R3S6) [NBP3-16528] - Confocal imaging of paraffin-embedded Human colon cancer tissue using HSP90 alpha Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.](http://images.novusbio.com/fullsize/nbp3-16528_rabbit-hsp90-alpha-mab-0r3s6-5220251255590.jpg)
![Western Blot: FAM59A Antibody [NBP2-81933] - Analysis of FAM59A in rat liver tissue lysate with FAM59A antibody at 1 ug/ml in the (A) absence and (B) presence of blocking peptide.](http://images.novusbio.com/fullsize/FAM59A-Antibody-Western-Blot-NBP2-81933-img0001.jpg "Western Blot: FAM59A Antibody [NBP2-81933] - Analysis of FAM59A in rat liver tissue lysate with FAM59A antibody at 1 ug/ml in the (A) absence and (B) presence of blocking peptide.")
![Immunohistochemistry: FAM59A Antibody [NBP2-81933] - Analysis of FAM59A in human brain tissue with FAM59A antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/FAM59A-Antibody-Immunohistochemistry-NBP2-81933-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: FAM59A Antibody - BSA Free [NBP2-81933] - Immunofluorescence of FAM59A in Human Liver cells with FAM59A antibody at 20 u/mL.](http://images.novusbio.com/fullsize/nbp2-81933_rabbit-polyclonal-fam59a-antibody-2711202419241337.jpg)
![Immunocytochemistry/Immunofluorescence: RhoA Antibody [NBP3-15485] - Immunofluorescence analysis of U2OS cells using RhoA antibody (NBP3-15485) at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/RhoA-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-15485-img0003.jpg "Immunocytochemistry/Immunofluorescence: RhoA Antibody [NBP3-15485] - Immunofluorescence analysis of U2OS cells using RhoA antibody (NBP3-15485) at dilution of 1:100. Blue: DAPI for nuclear staining.")
![Immunohistochemistry-Paraffin: RhoA Antibody [NBP3-15485] - Human colon carcinoma using [KO Validated] RhoA Rabbit pAb (NBP3-15485) at dilution of 1:350 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/RhoA-Antibody-Immunohistochemistry-Paraffin-NBP3-15485-img0002.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: RhoA Antibody [NBP3-15485] - Western blot analysis of extracts from normal (control) and RhoA knockout (KO) HeLa cells, using RhoA antibody (NBP3-15485) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/RhoA-Antibody-Knockout-Validated-NBP3-15485-img0001.jpg)