| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-23558-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-04967-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB200-204SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-00249-50ul | |||
![Western Blot: Mcl-1 Antibody (OTI10F6) - Azide and BSA Free [NBP2-72598] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MCL-1.](http://images.novusbio.com/fullsize/Mcl-1-Antibody-OTI10F6-Azide-and-BSA-Free-Western-Blot-NBP2-72598-img0002.jpg "Western Blot: Mcl-1 Antibody (OTI10F6) - Azide and BSA Free [NBP2-72598] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MCL-1.")
![Immunocytochemistry/Immunofluorescence: Mcl-1 Antibody (OTI10F6) - Azide and BSA Free [NBP2-72598] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY MCL-1.](http://images.novusbio.com/fullsize/Mcl-1-Antibody-OTI10F6-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-72598-img0004.jpg)
![Flow Cytometry: Mcl-1 Antibody (OTI10F6) - Azide and BSA Free [NBP2-72598] - HEK293T cells transfected with either pCMV6-ENTRY MCL1 (Red) or empty vector control plasmid (Blue) were immunostained with anti-MCL1 mouse monoclonal and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/Mcl-1-Antibody-OTI10F6-Azide-and-BSA-Free-Flow-Cytometry-NBP2-72598-img0003.jpg)
![Western Blot: Mcl-1 Antibody (OTI10F6) - Azide and BSA Free [NBP2-72598] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MCL1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MCL1](http://images.novusbio.com/fullsize/Mcl-1-Antibody-OTI10F6-Azide-and-BSA-Free-Western-Blot-NBP2-72598-img0001.jpg)
![Western Blot: MEK2 Antibody (OTI1A2) - Azide and BSA Free [NBP2-72647] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MEK2.](http://images.novusbio.com/fullsize/MEK2-Antibody-OTI1A2-Azide-and-BSA-Free-Western-Blot-NBP2-72647-img0002.jpg "Western Blot: MEK2 Antibody (OTI1A2) - Azide and BSA Free [NBP2-72647] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MEK2.")
![Immunocytochemistry/Immunofluorescence: MEK2 Antibody (OTI1A2) - Azide and BSA Free [NBP2-72647] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY MEK2.](http://images.novusbio.com/fullsize/MEK2-Antibody-OTI1A2-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-72647-img0003.jpg)
![Western Blot: MEK2 Antibody (OTI1A2) - Azide and BSA Free [NBP2-72647] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/MEK2-Antibody-OTI1A2-Azide-and-BSA-Free-Western-Blot-NBP2-72647-img0001.jpg)
![Immunohistochemistry: Glucagon Antibody (4) - BSA Free [NBP2-23558] - Endocrine alpha cells from diabetic mice stained with (1:500). Courtesy of Dr. Annette Plesner, University of British Columbia](http://images.novusbio.com/fullsize/GLP1-Antibody-4-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-23558-img0001.jpg "Immunohistochemistry: Glucagon Antibody (4) - BSA Free [NBP2-23558] - Endocrine alpha cells from diabetic mice stained with (1:500). Courtesy of Dr. Annette Plesner, University of British Columbia")
![Glucagon Antibody (4) - BSA Free [NBP2-23558] - BSA Free [NBP2-23558]](http://images.novusbio.com/fullsize/GLP1-Antibody-4-BSA-Free-NBP2-23558-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: HDAC4+5+9 Antibody [NBP1-04967] - Staining of methanol-fixed Hela cells.](http://images.novusbio.com/fullsize/HDAC4+5+9-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-04967-img0001.jpg "Immunocytochemistry/Immunofluorescence: HDAC4+5+9 Antibody [NBP1-04967] - Staining of methanol-fixed Hela cells.")
![Western Blot: HDAC4+5+9 Antibody [NBP1-04967] - Western blot analysis of extracts from 293 and Hela cells using NBP1-04967](http://images.novusbio.com/fullsize/nbp1-04967_rabbit-polyclonal-hdac4-5-9-antibody-23420241523251.jpg)
![Immunohistochemistry-Paraffin: HDAC4+5+9 Antibody [NBP1-04967] - Paraffin-embedded human lung carcinoma tissue using NBP1-04967(left) or the same antibody preincubated with blocking peptide (right).](http://images.novusbio.com/fullsize/nbp1-04967_rabbit-polyclonal-hdac4-5-9-antibody-103202523464514.jpg)
![Western Blot: RELM beta Antibody [NB200-204] - WB detection of secreted mouse RELM-beta from the media of transfected HEK 293T cells with NB200-204 antibody at a dilution of 1:500.](http://images.novusbio.com/fullsize/RELM-beta-Antibody-Western-Blot-NB200-204-img0001.jpg "Western Blot: RELM beta Antibody [NB200-204] - WB detection of secreted mouse RELM-beta from the media of transfected HEK 293T cells with NB200-204 antibody at a dilution of 1:500.")
![Immunocytochemistry/Immunofluorescence: CTGF/CCN2 Antibody [NBP2-69990] - Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic/membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/CTGF-CCN2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-69990-img0004.jpg "Immunocytochemistry/Immunofluorescence: CTGF/CCN2 Antibody [NBP2-69990] - Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic/membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).")
![Immunohistochemistry: CTGF/CCN2 Antibody [NBP2-69990] - (6ug/ml) staining of paraffin embedded Rat Liver. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.](http://images.novusbio.com/fullsize/CTGF-CCN2-Antibody-Immunohistochemistry-NBP2-69990-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: CTGF/CCN2 Antibody [NBP2-69990] - Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic/membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/CTGF-CCN2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-69990-img0003.jpg)
![Control: CTGF/CCN2 Antibody [NBP2-69990] - Negative Control showing staining of paraffin embedded Rat Liver, with no primary antibody.](http://images.novusbio.com/fullsize/CTGF-CCN2-Antibody-Control-NBP2-69990-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: TRAIL R1/TNFRSF10A Antibody (DR-4-02) - Azide Free [NBP2-31343] - Staining (confocal microscopy) of HeLa human cervix carcinoma cell line transfected with TRAIL-R1 expression plasmid using anti-human TRAIL-R1 (DR-4-02).](http://images.novusbio.com/fullsize/TRAIL-R1-TNFRSF10A-Antibody-DR-4-02-Azide-Free-Immunofluorescence-NBP2-31343-img0001.jpg "Immunocytochemistry/Immunofluorescence: TRAIL R1/TNFRSF10A Antibody (DR-4-02) - Azide Free [NBP2-31343] - Staining (confocal microscopy) of HeLa human cervix carcinoma cell line transfected with TRAIL-R1 expression plasmid using anti-human TRAIL-R1 (DR-4-02).")
![Flow Cytometry: TRAIL R1/TNFRSF10A Antibody (DR-4-02) - Azide Free [NBP2-31343] - Using the PE direct conjugate Surface staining of CD261-transfected HEK-293 cells with anti-CD261/TRAIL-R1 (DR-4-02) PE.](http://images.novusbio.com/fullsize/TRAIL-R1-TNFRSF10A-Antibody-DR-4-02-Azide-Free-Flow-Cytometry-NBP2-31343-img0002.jpg)
![Immunoprecipitation: LSM1 Antibody [NBP3-00249] - LSM1 was immunoprecipitated using: Lane A:0.5 mg K562 Whole Cell Lysate 2 uL NBP3-00249 and 15 ul of 50 % Protein G agarose. Primary antibody: NBP3-00249, at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 15 kDa Observed band size: 15 kDa](http://images.novusbio.com/fullsize/nbp3-00249_rabbit-polyclonal-lsm1-antibody-235202521525043.png "Immunoprecipitation: LSM1 Antibody [NBP3-00249] - LSM1 was immunoprecipitated using: Lane A:0.5 mg K562 Whole Cell Lysate 2 uL NBP3-00249 and 15 ul of 50 % Protein G agarose. Primary antibody: NBP3-00249, at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 15 kDa Observed band size: 15 kDa")
![Immunohistochemistry-Paraffin: LSM1 Antibody [NBP3-00249] - Immunochemical staining of human LSM1 in human prostatic carcinoma with NBP3-00249 (1:10000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/nbp3-00249_rabbit-polyclonal-lsm1-antibody-235202511254418.png)
![Immunohistochemistry-Paraffin: LSM1 Antibody [NBP3-00249] - Immunochemical staining of human LSM1 in human lung carcinoma with NBP3-00249 (1:10000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/nbp3-00249_rabbit-polyclonal-lsm1-antibody-235202511545655.png)
![Western Blot: LSM1 Antibody [NBP3-00249] - NBP3-00249 at 1:500 dilution Lane A: K562 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:15 kDa Observed band size:15 kDa](http://images.novusbio.com/fullsize/nbp3-00249_rabbit-polyclonal-lsm1-antibody-235202511545611.png)
![Immunohistochemistry-Paraffin: LSM1 Antibody [NBP3-00249] - Immunochemical staining of human LSM1 in human breast carcinoma with NBP3-00249 (1:10000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/nbp3-00249_rabbit-polyclonal-lsm1-antibody-235202521525060.png)
![Western Blot: GATA-3 Antibody (A3G3-R) [NBP3-32375] - Western blot analysis of GATA-3 on different lysates with Mouse anti-GATA-3 antibody (NBP3-32375) at 1/1,000 dilution. Lane 1: SH-SY5Y cell lysate Lane 2: MCF7 cell lysate Lane 3: Jurkat cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 48 kDa Observed band size: 48/37/25 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32375) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32375_mouse-gata-3-mab-a3g3-r-27820241631676.jpg "Western Blot: GATA-3 Antibody (A3G3-R) [NBP3-32375] - Western blot analysis of GATA-3 on different lysates with Mouse anti-GATA-3 antibody (NBP3-32375) at 1/1,000 dilution. Lane 1: SH-SY5Y cell lysate Lane 2: MCF7 cell lysate Lane 3: Jurkat cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 48 kDa Observed band size: 48/37/25 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32375) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: GATA-3 Antibody (A3G3-R) [NBP3-32375] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Mouse anti-GATA-3 antibody (NBP3-32375) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32375) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32375_mouse-gata-3-mab-a3g3-r-298202414161838.jpg)
![Immunocytochemistry/ Immunofluorescence: GATA-3 Antibody (A3G3-R) [NBP3-32375] - Immunocytochemistry analysis of SH-SY5Y cells labeling GATA-3 with Mouse anti-GATA-3 antibody (NBP3-32375) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-GATA-3 antibody (NBP3-32375) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32375_mouse-gata-3-mab-a3g3-r-29202410234242.jpg)
![Flow Cytometry: GATA-3 Antibody (A3G3-R) [NBP3-32375] - Flow cytometric analysis of SH-SY5Y cells labeling GATA-3. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32375, 1μg/mL) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32375_mouse-gata-3-mab-a3g3-r-29202416372173.jpg)
![Western Blot: HLA G Antibody (5A6G7) [NBP1-44924] - Analysis of whole cell lysate of HLA-G stable transfectants (various splice variants) using anti-human HLA-G (5A6G7). Lane 1: M8 cell line transfected with empty vector Lane 2: M8 cell line transfected with HLA-G1 Lane 3: M8 cell line transfected with HLA-G5 Lane 4: M8 cell line transfected with HLA-G6](http://images.novusbio.com/fullsize/HLA-G-Antibody-5A6G7-Western-Blot-NBP1-44924-img0001.jpg "Western Blot: HLA G Antibody (5A6G7) [NBP1-44924] - Analysis of whole cell lysate of HLA-G stable transfectants (various splice variants) using anti-human HLA-G (5A6G7). Lane 1: M8 cell line transfected with empty vector Lane 2: M8 cell line transfected with HLA-G1 Lane 3: M8 cell line transfected with HLA-G5 Lane 4: M8 cell line transfected with HLA-G6")
![Flow Cytometry: HLA G Antibody (5A6G7) [NBP1-44924] - Staining of HLA-G in HLA-G1 transfectants with anti-HLA-G (5A6G7) biotin / streptavidin-APC.](http://images.novusbio.com/fullsize/HLA-G-Antibody-5A6G7-Flow-Cytometry-NBP1-44924-img0003.jpg)
![Flow Cytometry: HLA G Antibody (5A6G7) [NBP1-44924] - Staining of HLA-G in HLA-G6 transfectants with anti-HLA-G (5A6G7) PE.](http://images.novusbio.com/fullsize/HLA-G-Antibody-5A6G7-Flow-Cytometry-NBP1-44924-img0002.jpg)
![Western Blot: HDAC6 Antibody (236) [NBP3-05295] - Western blotting analysis of human HDAC6 by mouse monoclonal antibody NBP3-05295 in HEK-293T cell line under reducing conditions. PVDF membrane was blocked in 5% milk, and incubated with primary antibody (1 ug/ml), followed by HRP-conjugated anti-mouse secondary antibody.](http://images.novusbio.com/fullsize/HDAC6-Antibody-236-Western-Blot-NBP3-05295-img0004.jpg "Western Blot: HDAC6 Antibody (236) [NBP3-05295] - Western blotting analysis of human HDAC6 by mouse monoclonal antibody NBP3-05295 in HEK-293T cell line under reducing conditions. PVDF membrane was blocked in 5% milk, and incubated with primary antibody (1 ug/ml), followed by HRP-conjugated anti-mouse secondary antibody.")
![Immunocytochemistry/Immunofluorescence: HDAC6 Antibody (236) [NBP3-05295] - Immunocytochemistry staining of HDAC6 in formaldehyde-fixed and Triton-permeabilized HEK-293T cells (A) and SH-SY5Y cells (B) by mouse monoclonal antibody NBP3-05295, followed by anti-mouse Alexa Fluor 488 (green), DNA indicated by DAPI (blue). In SH-SY5Y cells tubulin was stained by a rabbit polyclonal antibody, followed by anti-rabbit Alexa Fluor 647 (red, colocalization yellow).](http://images.novusbio.com/fullsize/HDAC6-Antibody-236-Immunocytochemistry-Immunofluorescence-NBP3-05295-img0001.jpg)
![ELISA: HDAC6 Antibody (236) [NBP3-05295] - ELISA analysis of human HDAC6 by mouse monoclonal antibody NBP3-05295. Plate was coated with recombinant human HDAC6, then blocked with BSA, and exposed to dilution series of anti-HDAC6 primary antibody, followed by HRP-conjugated anti-mouse secondary antibody, and colorimetric signal of processed OPD substrate was measured at 492 nm.](http://images.novusbio.com/fullsize/HDAC6-Antibody-236-ELISA-NBP3-05295-img0002.jpg)
![Immunoprecipitation: HDAC6 Antibody (236) [NBP3-05295] - Immunoprecipitation of recombinant human HDAC6 by mouse monoclonal antibodies 178 and NBP3-05295 using protein G-coated Dynabeads. Eluted proteins were processed by SDS PAAGE under reducing conditions and visualized by Coomassie Brilliant Blue.](http://images.novusbio.com/fullsize/HDAC6-Antibody-236-Immunoprecipitation-NBP3-05295-img0003.jpg)
![Flow Cytometry: Mouse Monoclonal HDAC6 Antibody (236) [NBP3-05295] - Separation of K562 cells stained using anti-human HDAC6 (236) purified antibody (concentration in sample 5.33 ug/ml, GAM APC, red-filled) from K562 cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (intracellular staining).](http://images.novusbio.com/fullsize/antibody/nbp3-05295_mouse-monoclonal-hdac6-antibody-236-flow-cytometry-1112202412584..jpg)