| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89621-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-52645-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-27160-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-44779 | |||
![Western Blot: JNK2 Antibody (011) [NBP2-89621] - Lane A: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:48 kDa Observed band size:54 kDa (We are unsure as to the identity of these extra bands.)](http://images.novusbio.com/fullsize/JNK2-Antibody-011-Western-Blot-NBP2-89621-img0004.jpg "Western Blot: JNK2 Antibody (011) [NBP2-89621] - Lane A: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:48 kDa Observed band size:54 kDa (We are unsure as to the identity of these extra bands.)")
![Immunocytochemistry/Immunofluorescence: JNK2 Antibody (011) [NBP2-89621] - Staining of Human MAPK9 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Rabbit anti-Human MAPK9 monoclonal antibody (1:300) at 37c 1 hour. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm and Nucleus.](http://images.novusbio.com/fullsize/JNK2-Antibody-011-Immunocytochemistry-Immunofluorescence-NBP2-89621-img0001.jpg)
![Immunohistochemistry-Paraffin: JNK2 Antibody (011) [NBP2-89621] - Staining of human MAPK9 in human brain with rabbit mnoclonal antibody (1:1000).](http://images.novusbio.com/fullsize/JNK2-Antibody-011-Immunohistochemistry-Paraffin-NBP2-89621-img0003.jpg)
![Immunohistochemistry-Paraffin: JNK2 Antibody (011) [NBP2-89621] - Staining of human MAPK9 in human kidney with rabbit monoclonal antibody (1:1000).](http://images.novusbio.com/fullsize/JNK2-Antibody-011-Immunohistochemistry-Paraffin-NBP2-89621-img0002.jpg)
![Immunoprecipitation: JNK2 Antibody (011) [NBP2-89621] - Lane A:0.5 mg HepG2 Whole Cell Lysate Lane B:0.5 mg Hela Whole Cell LysateLane C:0.5 mg Jurkat Whole Cell Lysate Lane D:0.5 mg MCF-7 Whole Cell Lysate0.5 uL anti-MAPK9 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-MAPK9 rabbit monoclonal antibody,at 1:500 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 44 kDa Observed band size: 44 kDa](http://images.novusbio.com/fullsize/JNK2-Antibody-011-Immunoprecipitation-NBP2-89621-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: JNK2 Antibody (011) [NBP2-89621] - Staining of Human MAPK9 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-89621 antibody (1:300) at 37c 1 hour. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm and Nucleus.](http://images.novusbio.com/fullsize/nbp2-89621_rabbit-monoclonal-jnk2-antibody-011-23520251544322.png)
![Western Blot: Alkaline Phosphatase/ALPP Antibody (JM22-53) [NBP2-67136] - Analysis of Placental alkaline phosphatase/ALPP on human placenta tissue lysates with Rabbit anti-Placental alkaline phosphatase/ALPP antibody at 1/500 dilution. Lysates/proteins at 20 ug/Lane. Predicted band size: 58 kDa Observed band size: 70 kDa Exposure time: 30 seconds; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-ALPP-Antibody-JM22-53-Western-Blot-NBP2-67136-img0005.jpg "Western Blot: Alkaline Phosphatase/ALPP Antibody (JM22-53) [NBP2-67136] - Analysis of Placental alkaline phosphatase/ALPP on human placenta tissue lysates with Rabbit anti-Placental alkaline phosphatase/ALPP antibody at 1/500 dilution. Lysates/proteins at 20 ug/Lane. Predicted band size: 58 kDa Observed band size: 70 kDa Exposure time: 30 seconds; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: Alkaline Phosphatase/ALPP Antibody (JM22-53) [NBP2-67136] - Staining PLAP in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-ALPP-Antibody-JM22-53-Immunocytochemistry-Immunofluorescence-NBP2-67136-img0002.jpg)
![Immunohistochemistry-Paraffin: Alkaline Phosphatase/ALPP Antibody (JM22-53) [NBP2-67136] - Analysis of paraffin-embedded human seminoma tissue with Rabbit anti-Placental alkaline phosphatase/ALPP antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-ALPP-Antibody-JM22-53-Immunohistochemistry-Paraffin-NBP2-67136-img0007.jpg)
![Western Blot: Alkaline Phosphatase/ALPP Antibody (JM22-53) [NBP2-67136] - Analysis of PLAP on human placenta tissue (1) and Hela cell (2) lysate using anti-PLAP antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-ALPP-Antibody-JM22-53-Western-Blot-NBP2-67136-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Alkaline Phosphatase/ALPP Antibody (JM22-53) [NBP2-67136] - Staining PLAP in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-ALPP-Antibody-JM22-53-Immunocytochemistry-Immunofluorescence-NBP2-67136-img0001.jpg)
![Immunohistochemistry-Paraffin: Alkaline Phosphatase/ALPP Antibody (JM22-53) [NBP2-67136] - Analysis of paraffin-embedded human placenta tissue using anti-PLAP antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-ALPP-Antibody-JM22-53-Immunohistochemistry-Paraffin-NBP2-67136-img0003.jpg)
![Immunohistochemistry-Paraffin: Alkaline Phosphatase/ALPP Antibody (JM22-53) [NBP2-67136] - Analysis of paraffin-embedded human placenta tissue with Rabbit anti-Placental alkaline phosphatase/ALPP antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-ALPP-Antibody-JM22-53-Immunohistochemistry-Paraffin-NBP2-67136-img0006.jpg)
![Western Blot: MDH1 Antibody (JE35-03) [NBP3-32582] - Western blot analysis of MDH1 on different lysates with Rabbit anti-MDH1 antibody (NBP3-32582) at 1/1,000 dilution. Lane 1: Jurkat cell lysate (20 ug/Lane) Lane 2: HepG2 cell lysate (20 ug/Lane) Lane 3: U-937 cell lysate (20 ug/Lane) Lane 4: HL-60 cell lysate (20 ug/Lane) Lane 5: NIH/3T3 cell lysate (20 ug/Lane) Lane 6: Rat kidney tissue lysate (40 ug/Lane) Predicted band size: 36 kDa Observed band size: 35 kDa Exposure time: 6 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32582) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32582_rabbit-mdh1-mab-je35-03-278202416331296.jpg)
![Immunocytochemistry/ Immunofluorescence: MDH1 Antibody (JE35-03) [NBP3-32582] - Immunocytochemistry analysis of U-937 cells labeling MDH1 with Rabbit anti-MDH1 antibody (NBP3-32582) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-MDH1 antibody (NBP3-32582) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32582_rabbit-mdh1-mab-je35-03-2920241114837.jpg)
![Flow Cytometry: MDH1 Antibody (JE35-03) [NBP3-32582] - Flow cytometric analysis of U-937 cells labeling MDH1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32582, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32582_rabbit-mdh1-mab-je35-03-392024884643.jpg)
![Western Blot: Vimentin Antibody (PDH0-01) [NBP3-33093] - Western blot analysis of Vimentin on different lysates with Rabbit anti-Vimentin antibody (NBP3-33093) at 1/5,000 dilution. Lane 1: L6 cell lysate Lane 2: C2C12 cell lysate Lane 3: HeLa cell lysate Lane 4: A549 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 54 kDa Observed band size: 57 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33093) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33093_rabbit-vimentin-mab-pdh0-01-288202414191539.jpg "Western Blot: Vimentin Antibody (PDH0-01) [NBP3-33093] - Western blot analysis of Vimentin on different lysates with Rabbit anti-Vimentin antibody (NBP3-33093) at 1/5,000 dilution. Lane 1: L6 cell lysate Lane 2: C2C12 cell lysate Lane 3: HeLa cell lysate Lane 4: A549 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 54 kDa Observed band size: 57 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33093) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Vimentin Antibody (PDH0-01) [NBP3-33093] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Vimentin antibody (NBP3-33093) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33093) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33093_rabbit-vimentin-mab-pdh0-01-29820241433367.jpg)
![Immunohistochemistry: Vimentin Antibody (PDH0-01) [NBP3-33093] - Immunofluorescence analysis of paraffin-embedded human kidney tissue labeling Vimentin with Rabbit anti-Vimentin antibody (NBP3-33093) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-33093, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-33093_rabbit-vimentin-mab-pdh0-01-298202414304144.jpg)
![Immunocytochemistry/ Immunofluorescence: Vimentin Antibody (PDH0-01) [NBP3-33093] - Immunocytochemistry analysis of HeLa cells labeling Vimentin with Rabbit anti-Vimentin antibody (NBP3-33093) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Vimentin antibody (NBP3-33093) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-33093_rabbit-vimentin-mab-pdh0-01-2920241114843.jpg)
![Flow Cytometry: Vimentin Antibody (PDH0-01) [NBP3-33093] - Flow cytometric analysis of Hela cells labeling Vimentin. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-33093, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-33093_rabbit-vimentin-mab-pdh0-01-392024884612.jpg)
![Dual RNAscope ISH-IHC: CD52 Antibody (Campath-1H) [NBP2-52645] - Formalin-fixed paraffin-embedded tissue sections of human tonsil were probed for CD52 mRNA (ACD RNAScope probe, catalog # 519381; Fast Red chromogen, ACD catalog # 322500) followed by immunohistochemistry using rabbit monoclonal (Novus catalog # NBP2-52645) at 5ug/mL for 1 hour at room temperature and detection with anti-mouse IgG VisUCyte HRP Polymer Antibody (R&D Systems Catalog # VC003) and green HRP chromogen (ACD Catalog # 322500).](http://images.novusbio.com/fullsize/CD52-Antibody-Campath-1H-Dual-RNAscope-ISH-IHC-NBP2-52645-img0010.jpg "<b>Orthogonal Strategies Validation. </b>Dual RNAscope ISH-IHC: CD52 Antibody (Campath-1H) [NBP2-52645] - Formalin-fixed paraffin-embedded tissue sections of human tonsil were probed for CD52 mRNA (ACD RNAScope probe, catalog # 519381; Fast Red chromogen, ACD catalog # 322500) followed by immunohistochemistry using rabbit monoclonal (Novus catalog # NBP2-52645) at 5ug/mL for 1 hour at room temperature and detection with anti-mouse IgG VisUCyte HRP Polymer Antibody (R&D Systems Catalog # VC003) and green HRP chromogen (ACD Catalog # 322500).")
![<b>Orthogonal Strategies Validation. </b>Flow Cytometry: CD52 Antibody (Campath-1H) [NBP2-52645] - Flow-cytometry using anti-CD52 antibody Campath-1H. Cynomolgus monkey lymphocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of Campath-1H (panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/CD52-Antibody-Campath-1H-NBP2-52645-img0005.jpg)
![Flow Cytometry: CD52 Antibody (Campath-1H) [NBP2-52645] - Flow-cytometry using anti-CD52 antibody Campath-1H. Rhesus monkey lymphocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of Campath-1H (panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/CD52-Antibody-Campath-1H-NBP2-52645-img0004.jpg)
![Flow Cytometry: SP17 Antibody (3G9F3) [NBP3-27160] - Analysis of SK-OV-3 cells using SP17 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/nbp3-27160_mouse-sp17-mab-3g9f3-bsa-free-10420249141928.jpg%3Fv=1666704829 "Flow Cytometry: SP17 Antibody (3G9F3) [NBP3-27160] - Analysis of SK-OV-3 cells using SP17 mouse mAb (green) and negative control (red).")
![Western Blot: SP17 Antibody (3G9F3) [NBP3-27160] - Analysis using SP17 mAb against human SP17 (AA: 1-152) recombinant protein. (Expected MW is 20.3 kDa)](http://images.novusbio.com/fullsize/nbp3-27160_mouse-sp17-mab-3g9f3-bsa-free-1042024915845.jpg%3Fv=1666704829)
![Immunohistochemistry: SP17 Antibody (3G9F3) [NBP3-27160] - Analysis of paraffin-embedded rectal cancer tissues using SP17 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/nbp3-27160_mouse-sp17-mab-3g9f3-bsa-free-104202491622.jpg%3Fv=1666704829)
![ELISA: SP17 Antibody (3G9F3) [NBP3-27160] - Black line: Control Antigen (100 ng); Purple line: Antigen (10 ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)](http://images.novusbio.com/fullsize/nbp3-27160_mouse-sp17-mab-3g9f3-bsa-free-1042024915840.jpg%3Fv=1666704829)
![Flow Cytometry: SP17 Antibody (3G9F3) [NBP3-27160] - Analysis of Hela cells using SP17 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/nbp3-27160_mouse-sp17-mab-3g9f3-bsa-free-104202491620.jpg%3Fv=1666704829)
![Immunohistochemistry: SP17 Antibody (3G9F3) [NBP3-27160] - Analysis of paraffin-embedded testis tissues using SP17 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/nbp3-27160_mouse-sp17-mab-3g9f3-bsa-free-1042024916228.jpg%3Fv=1666704830)
![Western Blot: SP17 Antibody (3G9F3) [NBP3-27160] - Analysis using SP17 mAb against HEK293-6e (1) and SP17 (AA: 1-152)-hIgGFc transfected HEK293-6e (2) cell lysate.](http://images.novusbio.com/fullsize/nbp3-27160_mouse-sp17-mab-3g9f3-bsa-free-1042024918197.jpg%3Fv=1666704829)
![Western Blot: Bcl-2 Antibody (8C8) - Azide and BSA Free [NBP2-33313] - Western blot of Bcl-2 in human A) HeLa B) A549 and C) MCF7 Lysate using Bcl-2 Monoclonal Antibody (8C8)](http://images.novusbio.com/fullsize/Bcl-2-Antibody-8C8-Azide-and-BSA-Free-Western-Blot-NBP2-33313-img0003.jpg "Western Blot: Bcl-2 Antibody (8C8) - Azide and BSA Free [NBP2-33313] - Western blot of Bcl-2 in human A) HeLa B) A549 and C) MCF7 Lysate using Bcl-2 Monoclonal Antibody (8C8)")
![Immunohistochemistry-Paraffin: Bcl-2 Antibody (8C8) - Azide and BSA Free [NBP2-33313] - Formalin-fixed paraffin-embedded human tonsil stained with Bcl-2 Monoclonal Antibody (8C8) Note cell membrane and cytoplasmic staining](http://images.novusbio.com/fullsize/Bcl-2-Antibody-8C8-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-33313-img0002.jpg)
![Western Blot: Bcl-2 Antibody (8C8) - Azide and BSA Free [NBP2-33313] - Analysis of Bcl-2 in 293T cell lsyate (30ug) using Bcl-2 HRP conjugated antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/Bcl-2-Antibody-8C8-Azide-and-BSA-Free-Western-Blot-NBP2-33313-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: EpCAM/TROP1 Antibody (EGP40/1384) - (ECD) - Azide and BSA Free [NBP2-54333] - Confocal Immunofluorescence of MCF-7 cells. EpCAM/TROP1 Antibody (EGP40/1384). labeled with CF488 (Green); Red Dot is used to label the nuclei.](http://images.novusbio.com/fullsize/EpCAM-TROP1-Antibody-EGP40-1384-ECD-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-54333-img0003.jpg "Immunocytochemistry/Immunofluorescence: EpCAM/TROP1 Antibody (EGP40/1384) - (ECD) - Azide and BSA Free [NBP2-54333] - Confocal Immunofluorescence of MCF-7 cells. EpCAM/TROP1 Antibody (EGP40/1384). labeled with CF488 (Green); Red Dot is used to label the nuclei.")
![Immunohistochemistry-Paraffin: EpCAM/TROP1 Antibody (EGP40/1384) - (ECD) - Azide and BSA Free [NBP2-54333] - Formalin-fixed, paraffin-embedded feline (cat) small intestine stained with EpCAM/TROP1 Antibody (EGP40/1384) - (ECD).](http://images.novusbio.com/fullsize/EpCAM-TROP1-Antibody-EGP40-1384-ECD-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-54333-img0004.jpg)
![Immunohistochemistry-Paraffin: EpCAM/TROP1 Antibody (EGP40/1384) - (ECD) - Azide and BSA Free [NBP2-54333] - Formalin-fixed, paraffin-embedded Human Ovarian Carcinoma stained with EpCAM Monoclonal Antibody (EGP40/1384).](http://images.novusbio.com/fullsize/EpCAM-TROP1-Antibody-EGP40-1384-ECD-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-54333-img0001.jpg)
![Protein Array: EpCAM/TROP1 Antibody (EGP40/1384) - (ECD) - Azide and BSA Free [NBP2-54333] - Analysis of Protein Array containing >19,000 full-length human proteins using EpCAM/TROP1 Antibody (EGP40/1384) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SDs) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SDs) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/EpCAM-TROP1-Antibody-EGP40-1384-ECD-Azide-and-BSA-Free-Protein-Array-NBP2-54333-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: ErbB2/Her2 Antibody (HRB2/451) - Azide and BSA Free [NBP2-33064] - Immunofluorescent staining of PFA-fixed MCF-7 cells with ErbB2/Her2 Monospecific Mouse Monoclonal Antibody (HRB2/451) followed by goat anti-Mouse IgG-CF488 (Green). Nuclei are stained with Reddot (Red).](http://images.novusbio.com/fullsize/ErbB2-Her2-Antibody-HRB2-451-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-33064-img0007.jpg "Immunocytochemistry/Immunofluorescence: ErbB2/Her2 Antibody (HRB2/451) - Azide and BSA Free [NBP2-33064] - Immunofluorescent staining of PFA-fixed MCF-7 cells with ErbB2/Her2 Monospecific Mouse Monoclonal Antibody (HRB2/451) followed by goat anti-Mouse IgG-CF488 (Green). Nuclei are stained with Reddot (Red).")
![Immunocytochemistry/Immunofluorescence: ErbB2/Her2 Antibody (HRB2/451) - Azide and BSA Free [NBP2-33064] - Immunofluorescent staining of MCF-7 cells with ErbB2/Her2 Antibody (HRB2/451) followed by goat anti-Mouse IgG-CF488 (Green). Nuclei are stained with Red Dot (Red).](http://images.novusbio.com/fullsize/ErbB2-Her2-Antibody-HRB2-451-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-33064-img0004.jpg)
![Flow Cytometry: ErbB2/Her2 Antibody (HRB2/451) - Azide and BSA Free [NBP2-33064] - Flow Cytometric Analysis of human trypsinized SK-BR3 cells. ErbB2/Her2 Antibody (HRB2/451) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).](http://images.novusbio.com/fullsize/ErbB2-Her2-Antibody-HRB2-451-Azide-and-BSA-Free-Flow-Cytometry-NBP2-33064-img0002.jpg)
![Flow Cytometry: ErbB2/Her2 Antibody (HRB2/451) - Azide and BSA Free [NBP2-33064] - Flow Cytometric Analysis of human trypsinized MCF-7 cells. ErbB2/Her2 Monospecific Mouse Monoclonal Antibody (HRB2/451) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).](http://images.novusbio.com/fullsize/ErbB2-Her2-Antibody-HRB2-451-Azide-and-BSA-Free-Flow-Cytometry-NBP2-33064-img0008.jpg)
![Protein Array: ErbB2/Her2 Antibody (HRB2/451) - Azide and BSA Free [NBP2-33064] - Analysis of Protein Array containing more than 19,000 full-length human proteins using ErbB2/Her2 Antibody (HRB2/451) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SDs) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SDs) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/ErbB2-Her2-Antibody-HRB2-451-Azide-and-BSA-Free-Protein-Array-NBP2-33064-img0003.jpg)
![Western Blot: HeLa Chloroquine Treated / Untreated Cell Lysate [NBP2-49689] - HeLa cells were treated with (+) or without 50 uM (-) of Chloriquine (CQ) for 24 hours. Total cell lysates were prepared and separated on a 12% gel by SDS-PAGE. Protein was transferred to PVDF membrane and blocked in 5% non-fat milk. The membrane was then probed with 2 ug/ml of p62/SQSTM1 antibody (NBP1-42821) in 1% milk and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. Note the upregulation of p62 (arrowhead) in response to chloroquine treatment and the blockage of autophagy.](http://images.novusbio.com/fullsize/HeLa-Chloroquine-Treated---Untreated-Cell-Lysate-Western-Blot-NBP2-49689-img0005.jpg "Western Blot: HeLa Chloroquine Treated / Untreated Cell Lysate [NBP2-49689] - HeLa cells were treated with (+) or without 50 uM (-) of Chloriquine (CQ) for 24 hours. Total cell lysates were prepared and separated on a 12% gel by SDS-PAGE. Protein was transferred to PVDF membrane and blocked in 5% non-fat milk. The membrane was then probed with 2 ug/ml of p62/SQSTM1 antibody (NBP1-42821) in 1% milk and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. Note the upregulation of p62 (arrowhead) in response to chloroquine treatment and the blockage of autophagy.")
![<b>Biological Strategies Validation. </b>Western Blot: HeLa Chloroquine Treated / Untreated Cell Lysate [NBP2-49689] - 10 ug of Chloroquine treated (+) and untreated (-) HeLa lysates in 1x Laemmli sample buffer (NBP2-49689) were separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1 ug/ml monoclonal anti-LC3 (NBP2--46892) and detected with an anti-rabbit secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/HeLa-Chloroquine-Treated---Untreated-Cell-Lysate-Western-Blot-NBP2-49689-img0002.jpg)
![Western Blot: HeLa Chloroquine Treated / Untreated Cell Lysate [NBP2-49689] - Human Cervical carcinoma cells (HeLa) were treated with (+) and without (-) 50 uM Chloroquine overnight. Whole cell protein lysates were prepared in 1x Laemmli sample buffer and approximately 10 ug of each lysate (NBP2-49689) was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1 ug/ml anti-LC3 (NB100-2220) and 1 ug/ml anti-alpha tubulin (NB100-690) as a loading control, and detected with the appropriate secondary antibodies using chemiluminescence.](http://images.novusbio.com/fullsize/HeLa-Chloroquine-Treated---Untreated-Cell-Lysate-Western-Blot-NBP2-49689-img0003.jpg)
![Western Blot: HeLa Chloroquine Treated / Untreated Cell Lysate [NBP2-49689] - HeLa cells were treated with vehicle (-) or with 50 uM chloriquine (+) for 24 hours and the total cell lysates were prepared. The lysates were separated on a 12% gel by SDS-PAGE. Protein was transferred to PVDF mebbrane and blocked in 5% non-fat milk. The membrane was then probed with 1 ug/ml of p62/SQSTM1 antibody (NBP1-48320) in 1% milk and dectected with an anti-rabbit HRP secondary antibody using chemiluminescence. Note the upregulation of p62 (arrowhead) in response to chloroquine treatment and the blockage of autophagy.](http://images.novusbio.com/fullsize/HeLa-Chloroquine-Treated---Untreated-Cell-Lysate-Western-Blot-NBP2-49689-img0004.jpg)
![Western Blot: Matriptase/ST14 Antibody [NB100-2308] - Detection of Human Matriptase/ST14 by Western Blot. Samples: Whole cell lysate (50 ug) from MCF7, OVCAR4 [OV], SW620 [SW], RPMI8266 [RPMI], and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Matriptase/ST14 antibody NB100-2308 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/Matriptase-ST14-Antibody-Western-Blot-NB100-2308-img0003.jpg "Western Blot: Matriptase/ST14 Antibody [NB100-2308] - Detection of Human Matriptase/ST14 by Western Blot. Samples: Whole cell lysate (50 ug) from MCF7, OVCAR4 [OV], SW620 [SW], RPMI8266 [RPMI], and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Matriptase/ST14 antibody NB100-2308 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: Matriptase/ST14 Antibody [NB100-2308] - Detection of human Matriptase/ST14 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from MCF-7 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Matriptase/ST14 antibody NB100-2308 used for IP at 6 ug per reaction. Matriptase/ST14 was also immunoprecipitated by Rabbit anti-Matriptase/ST14 antibodies A, B and C. For blotting immunoprecipitated Matriptase/ST14, NB100-2308 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/Matriptase-ST14-Antibody-Immunoprecipitation-NB100-2308-img0004.jpg)