| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Allophycocyanin | NB7171APC | |||
| Biotin | NB7171B | |||
| DyLight 350 | NB7171UV | |||
| DyLight 405 | NB7171V | |||
| DyLight 488 | NB7171G | |||
| DyLight 550 | NB7171R | |||
| DyLight 594 | NB7171DL594 | |||
| DyLight 650 | NB7171C | |||
| DyLight 680 | NB7171FR | |||
| DyLight 755 | NB7171IR | |||
| FITC | NB7173 | |||
| HRP | NB7174 | |||
| Janelia Fluor 525 | NB7171JF525 | |||
| Janelia Fluor 669 | NB7171JF669 | |||
| PE | NB7171PE | |||
| PerCP | NB7171PCP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-43461-0.5mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35805-20ul | |||
![Flow Cytometry: CD1d Antibody (1B1) [NBP1-43461] - Analysis using the Biotin conjugate of NBP1-43461. Staining of BALB/c splenocytes with Anti-Human/Mouse CD45R (B220) FITC and 0.5 ug of Rat IgG2b kappa Isotype Control Biotin (left) or 0.5 ug of Anti-Mouse CD1d Biotin (right) followed by Streptavidin PE.](http://images.novusbio.com/fullsize/CD1d-Antibody-1B1-Flow-Cytometry-NBP1-43461-img0003.jpg "Flow Cytometry: CD1d Antibody (1B1) [NBP1-43461] - Analysis using the Biotin conjugate of NBP1-43461. Staining of BALB/c splenocytes with Anti-Human/Mouse CD45R (B220) FITC and 0.5 ug of Rat IgG2b kappa Isotype Control Biotin (left) or 0.5 ug of Anti-Mouse CD1d Biotin (right) followed by Streptavidin PE.")
![Flow Cytometry: CD1d Antibody (1B1) [NBP1-43461] - Staining of BALB/c splenocytes with 1.0 ug of Rat IgG2b Isotype Control Purified (open histogram) or 1.0 ug of Anti-Mouse CD1d Purified (filled histogram) followed by Anti-Rat IgG FITC . Total viable cells were used for analysis.](http://images.novusbio.com/fullsize/CD1d-Antibody-1B1-Flow-Cytometry-NBP1-43461-img0001.jpg)
![Immunoprecipitation: CD25/IL-2R alpha Antibody [NBP3-14733] - Whole cell lysate (0.5 mgper IP reaction; 5% of IP loaded) from MJ cells preparedusing NETN lysis buffer. Antibodies: Rabbit antiCD25/IL-2R alpha recombinant monoclonal antibody used for IP at 20 ul/mg lysate.CD25/IL-2R alpha was also immunoprecipitated by anotherantibody against a different epitope of CD25/IL-2R alpha. For blotting immunoprecipitatedCD25/IL-2R alpha, was used at 1:1000.Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/CD25-IL-2R-alpha-Antibody-Immunoprecipitation-NBP3-14733-img0001.jpg "Immunoprecipitation: CD25/IL-2R alpha Antibody [NBP3-14733] - Whole cell lysate (0.5 mgper IP reaction; 5% of IP loaded) from MJ cells preparedusing NETN lysis buffer. Antibodies: Rabbit antiCD25/IL-2R alpha recombinant monoclonal antibody used for IP at 20 ul/mg lysate.CD25/IL-2R alpha was also immunoprecipitated by anotherantibody against a different epitope of CD25/IL-2R alpha. For blotting immunoprecipitatedCD25/IL-2R alpha, was used at 1:1000.Chemiluminescence with an exposure time of 30 seconds.")
![Immunocytochemistry/ Immunofluorescence: TBC1D4 Antibody [NBP3-35805] - Immunofluorescence analysis of L929 cells using TBC1D4 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35805_rabbit-tbc1d4-pab-912202414213796.jpg "Immunocytochemistry/ Immunofluorescence: TBC1D4 Antibody [NBP3-35805] - Immunofluorescence analysis of L929 cells using TBC1D4 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Western Blot: TBC1D4 Antibody [NBP3-35805] - Western blot analysis of lysates from Rat skeletal muscle, using TBC1D4 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-35805_rabbit-tbc1d4-pab-912202415133469.jpg)
![Western Blot: Goat anti-Monkey IgM Heavy Chain Secondary Antibody [Biotin] [NBP1-73556] - Lane 1: Monkey IgM. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Biotin goat secondary antibody at 1:1,000 for 60 min at RT. Block: incubated with blocking buffer for 30 min t RT. Predicted/Observed size: 72 kDa, 72 kDa for Monkey IgM. Other band(s): None.](http://images.novusbio.com/fullsize/Goat-anti-Monkey-IgM-Heavy-Chain-Secondary-Antibody-[Biotin]-Western-Blot-NBP1-73556-img0003.jpg "Western Blot: Goat anti-Monkey IgM Heavy Chain Secondary Antibody [Biotin] [NBP1-73556] - Lane 1: Monkey IgM. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Biotin goat secondary antibody at 1:1,000 for 60 min at RT. Block: incubated with blocking buffer for 30 min t RT. Predicted/Observed size: 72 kDa, 72 kDa for Monkey IgM. Other band(s): None.")
![Western Blot: Rabbit anti-Rat IgM Heavy Chain Secondary Antibody [Rhodamine] [NBP1-73379] - Lane 1: Rat IgG whole molecule. Lane 2: Rat IgG F(c) Fragment. Lane 3: Rat IgG Fab Fragment. Lane 4: Rat IgM Whole Molecule. Lane 5: Rat Serum. All samples were reduced. Load: 50 ng per lane. Block: incubated with blocking buffer for 30 min at RT. Primary Antibody: Anti-Rat IgM (mu chain) (RABBIT) Antibody 1:2,000 for 60 min at RT. Secondary Antibody: Anti-Rabbit IgG (GOAT) Peroxidase Conjugated Antibody 1:40,000 in incubated with blocking buffer for 30 min at RT. Predicted/Observed Size: 25 and 55 kDa for Rat IgG and Serum, 25 kDa for F(c) and Fab, 78 and 25 kDa for IgM. Rat F(c) migrates slightly higher.](http://images.novusbio.com/fullsize/Rabbit-anti-Rat-IgM-Secondary-Antibody-[Rhodamine]-Western-Blot-NBP1-73379-img0004.jpg "Western Blot: Rabbit anti-Rat IgM Heavy Chain Secondary Antibody [Rhodamine] [NBP1-73379] - Lane 1: Rat IgG whole molecule. Lane 2: Rat IgG F(c) Fragment. Lane 3: Rat IgG Fab Fragment. Lane 4: Rat IgM Whole Molecule. Lane 5: Rat Serum. All samples were reduced. Load: 50 ng per lane. Block: incubated with blocking buffer for 30 min at RT. Primary Antibody: Anti-Rat IgM (mu chain) (RABBIT) Antibody 1:2,000 for 60 min at RT. Secondary Antibody: Anti-Rabbit IgG (GOAT) Peroxidase Conjugated Antibody 1:40,000 in incubated with blocking buffer for 30 min at RT. Predicted/Observed Size: 25 and 55 kDa for Rat IgG and Serum, 25 kDa for F(c) and Fab, 78 and 25 kDa for IgM. Rat F(c) migrates slightly higher.")