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![Western Blot: Goat anti-Rat IgM Heavy Chain Secondary Antibody [Biotin] [NBP1-73679] - Lane 1: Rat IgG whole molecule. Lane 2: Rat IgG F(c) Fragment. Lane 3: Rat IgG Fab Fragment. Lane 4: Rat IgM Whole Molecule. Lane 5: Rat Serum. All samples were reduced. Load: 50 ng per lane. Block: incubated with blocking buffer for 30 min at RT. Primary Antibody: Anti-Rat IgM (mu chain) (GOAT) Antibody 1:1,000 for 60 min at RT. Secondary Antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody 1:40,000 in incubated with blocking buffer for 30 min at RT. Predicted/Observed Size: 25 and 55 kDa for Rat IgG and Serum, 25 kDa for F(c) and Fab, 78 and 25 kDa for IgM. Rat F(c) migrates slightly higher.](http://images.novusbio.com/fullsize/Goat-anti-Rat-IgM-Secondary-Antibody-[Biotin]-Western-Blot-NBP1-73679-img0004.jpg "Western Blot: Goat anti-Rat IgM Heavy Chain Secondary Antibody [Biotin] [NBP1-73679] - Lane 1: Rat IgG whole molecule. Lane 2: Rat IgG F(c) Fragment. Lane 3: Rat IgG Fab Fragment. Lane 4: Rat IgM Whole Molecule. Lane 5: Rat Serum. All samples were reduced. Load: 50 ng per lane. Block: incubated with blocking buffer for 30 min at RT. Primary Antibody: Anti-Rat IgM (mu chain) (GOAT) Antibody 1:1,000 for 60 min at RT. Secondary Antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody 1:40,000 in incubated with blocking buffer for 30 min at RT. Predicted/Observed Size: 25 and 55 kDa for Rat IgG and Serum, 25 kDa for F(c) and Fab, 78 and 25 kDa for IgM. Rat F(c) migrates slightly higher.")
![Dot Blot: Rabbit anti-Canine IgM Heavy Chain Secondary Antibody [HRP] [NBP1-73847] - Dot Blot results of Rabbit anti-Canine IgM Heavy Chain Secondary antibody [HRP].Dots are Dog IgM at (1) 100ng, (2) 33.3ng, (3) 11.1ng, (4) 3.70ng, (5) 1.23ng. Blocking: 30 min at RT.Primary Antibody: none.Secondary Antibody: Rabbit Anti-Dog IgM -HRP at 1ug/mL for 1hr at RT.](http://images.novusbio.com/fullsize/Rabbit-anti-Canine-IgM-Heavy-Chain-Secondary-Antibody-[HRP]-Dot-Blot-NBP1-73847-img0001.jpg "Dot Blot: Rabbit anti-Canine IgM Heavy Chain Secondary Antibody [HRP] [NBP1-73847] - Dot Blot results of Rabbit anti-Canine IgM Heavy Chain Secondary antibody [HRP].Dots are Dog IgM at (1) 100ng, (2) 33.3ng, (3) 11.1ng, (4) 3.70ng, (5) 1.23ng. Blocking: 30 min at RT.Primary Antibody: none.Secondary Antibody: Rabbit Anti-Dog IgM -HRP at 1ug/mL for 1hr at RT.")
![Western Blot: Goat anti-Chicken IgM Heavy Chain Secondary Antibody [NBP2-60690] - Lane 1: Chicken IgG whole molecule. Lane 2: Chicken IgG F(c) Fragment. Lane 3: Chicken IgG F(ab) Fragment. Lane 4: Chicken IgM Whole Molecule. All samples were reduced. Load: 50 ng per lane. Block: incubated with blocking buffer for 30 min at RT. Primary Antibody: Anti-Chicken IgM (mu chain) (GOAT) Antibody Peroxidase Conjugated 1:30,000 for 60 min at RT. Predicted/Observed Size: 25 and 72 kDa for Chicken IgG, 25 kDa for F(c) and Fab, 75 kDa for IgM. Chicken F(c) migrates slightly higher. Image using the Biotin form of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Chicken-IgM-Heavy-Chain-Secondary-Antibody-Western-Blot-NBP2-60690-img0001.jpg "Western Blot: Goat anti-Chicken IgM Heavy Chain Secondary Antibody [NBP2-60690] - Lane 1: Chicken IgG whole molecule. Lane 2: Chicken IgG F(c) Fragment. Lane 3: Chicken IgG F(ab) Fragment. Lane 4: Chicken IgM Whole Molecule. All samples were reduced. Load: 50 ng per lane. Block: incubated with blocking buffer for 30 min at RT. Primary Antibody: Anti-Chicken IgM (mu chain) (GOAT) Antibody Peroxidase Conjugated 1:30,000 for 60 min at RT. Predicted/Observed Size: 25 and 72 kDa for Chicken IgG, 25 kDa for F(c) and Fab, 75 kDa for IgM. Chicken F(c) migrates slightly higher. Image using the Biotin form of this antibody.")
![SDS-Page: Goat anti-Chicken IgM Heavy Chain Secondary Antibody [NBP2-60690] - Lane 1: Goat anti-Chicken IgM Secondary Antibody - Non-Reduced. Lane 2: Goat anti-Chicken IgM Secondary Antibody - Reduced. Load: 1.0 ug per lane. Predicted/Observed size: 50 kDa, 25 kDa for Reduced - Goat IgG. Other band(s): none.](http://images.novusbio.com/fullsize/Goat-anti-Chicken-IgM-Heavy-Chain-Secondary-Antibody-SDS-Page-NBP2-60690-img0002.jpg)
![SDS-Page: Rat IgM Isotype Control [NBP1-96776] - Lane M: 3 uL Opal Prestained Marker. Lane 1: Reduced Rat IgG Whole Molecule. Lane 2: Reduced Rat IgG F(c) Fragment. Lane 3: Reduced Rat IgG Fab Fragment. Lane 4: Reduced Rat IgM Whole Molecule. Load: 1 ug of IgG, F(c), Fab; 1.5 ug of IgM.](http://images.novusbio.com/fullsize/Rat-IgM-Isotype-Control-SDS-Page-NBP1-96776-img0001.jpg "SDS-Page: Rat IgM Isotype Control [NBP1-96776] - Lane M: 3 uL Opal Prestained Marker. Lane 1: Reduced Rat IgG Whole Molecule. Lane 2: Reduced Rat IgG F(c) Fragment. Lane 3: Reduced Rat IgG Fab Fragment. Lane 4: Reduced Rat IgM Whole Molecule. Load: 1 ug of IgG, F(c), Fab; 1.5 ug of IgM.")
![SDS-Page: Rat IgM Isotype Control [NBP1-96776] - Lane 1: Rat IgM, Non-Reduced. Lane 2: Rat IgM, Reduced. Load: 1.0 ug per lane.](http://images.novusbio.com/fullsize/Rat-IgM-Isotype-Control-SDS-Page-NBP1-96776-img0002.jpg)
![Western Blot: Rabbit anti-Rat IgM Heavy Chain Secondary Antibody [NBP1-72790] - Lane 1: Rat IgG whole molecule. Lane 2: Rat IgG F(c) Fragment. Lane 3: Rat IgG Fab Fragment. Lane 4: Rat IgM Whole Molecule. Lane 5: Rat Serum. All samples were reduced. Load: 50 ng per lane. Block: incubated with blocking buffer for 30 min at RT. Primary Antibody: Anti-Rat IgM (mu chain) (RABBIT) Antibody 1:2,000 for 60 min at RT. Secondary Antibody: Anti-Rabbit IgG (GOAT) Peroxidase Conjugated Antibody 1:40,000 in incubated with blocking buffer for 30 min at RT. Predicted/Observed Size: 25 and 55 kDa for Rat IgG and Serum, 25 kDa for F(c) and Fab, 78 and 25 kDa for IgM. Rat F(c) migrates slightly higher.](http://images.novusbio.com/fullsize/Rabbit-anti-Rat-IgM-Secondary-Antibody-[Unconjugated]-Western-Blot-NBP1-72790-img0004.jpg "Western Blot: Rabbit anti-Rat IgM Heavy Chain Secondary Antibody [NBP1-72790] - Lane 1: Rat IgG whole molecule. Lane 2: Rat IgG F(c) Fragment. Lane 3: Rat IgG Fab Fragment. Lane 4: Rat IgM Whole Molecule. Lane 5: Rat Serum. All samples were reduced. Load: 50 ng per lane. Block: incubated with blocking buffer for 30 min at RT. Primary Antibody: Anti-Rat IgM (mu chain) (RABBIT) Antibody 1:2,000 for 60 min at RT. Secondary Antibody: Anti-Rabbit IgG (GOAT) Peroxidase Conjugated Antibody 1:40,000 in incubated with blocking buffer for 30 min at RT. Predicted/Observed Size: 25 and 55 kDa for Rat IgG and Serum, 25 kDa for F(c) and Fab, 78 and 25 kDa for IgM. Rat F(c) migrates slightly higher.")
![SDS-Page: Porcine IgM Isotype Control [NBP1-96788] - Lane 1: Swine IgM, 1.0 ug, Non-reduced. Lane 2: Swine IgM, 5.0 ug, Non-reduced. Lane 3: Swine IgM, 10.0 ug, Non-reduced. M: Opal Pre-stained Marker. Lane 4: Swine IgM, 1.0 ug, Reduced. Lane 5: Swine IgM, 5.0 ug, Reduced. Lane 6: Swine IgM, 10.0 ug, Reduced. Predicted/Observed size - Non-Reduced: 900 kDa, Reduced - 75, 25 kDa.](http://images.novusbio.com/fullsize/Porcine-IgM-Isotype-Control-SDS-Page-NBP1-96788-img0001.jpg "SDS-Page: Porcine IgM Isotype Control [NBP1-96788] - Lane 1: Swine IgM, 1.0 ug, Non-reduced. Lane 2: Swine IgM, 5.0 ug, Non-reduced. Lane 3: Swine IgM, 10.0 ug, Non-reduced. M: Opal Pre-stained Marker. Lane 4: Swine IgM, 1.0 ug, Reduced. Lane 5: Swine IgM, 5.0 ug, Reduced. Lane 6: Swine IgM, 10.0 ug, Reduced. Predicted/Observed size - Non-Reduced: 900 kDa, Reduced - 75, 25 kDa.")
![SDS-Page: Sheep IgM Isotype Control [NBP1-96774] - Lane 1: Sheep IgM, Non-Reduced. Lane 2: Sheep IgM, Reduced. Load: 1.0 ug per lane.](http://images.novusbio.com/fullsize/Sheep-IgM-Isotype-Control-SDS-Page-NBP1-96774-img0001.jpg "SDS-Page: Sheep IgM Isotype Control [NBP1-96774] - Lane 1: Sheep IgM, Non-Reduced. Lane 2: Sheep IgM, Reduced. Load: 1.0 ug per lane.")
![Flow Cytometry: Siglec-2/CD22 Antibody (RFB4) [NBP2-49890] - Flow Cytometry using Siglec-2/CD22 Antibody (RFB4).](http://images.novusbio.com/fullsize/Siglec-2-CD22-Antibody-RFB4-Flow-Cytometry-NBP2-49890-img0001.jpg "Flow Cytometry: Siglec-2/CD22 Antibody (RFB4) [NBP2-49890] - Flow Cytometry using Siglec-2/CD22 Antibody (RFB4).")
![Western Blot: gp96/HSP90B1/GRP94 Antibody (SR2295) [NBP3-21723] - Western blot analysis using NBP3-21723 in HeLa cell lysate.](http://images.novusbio.com/fullsize/nbp3-21723_rabbit-gp96-hsp90b1-grp94-mab-sr2295-6720239533762.jpg "Western Blot: gp96/HSP90B1/GRP94 Antibody (SR2295) [NBP3-21723] - Western blot analysis using NBP3-21723 in HeLa cell lysate.")