| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-94822-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-94900-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61853-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-00002-50ul | |||
![Western Blot: c-Myc Overexpression Lysate (Adult Normal) [NBL1-13414] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for c-Myc.](http://images.novusbio.com/fullsize/c-Myc-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-13414-img0002.jpg "Western Blot: c-Myc Overexpression Lysate (Adult Normal) [NBL1-13414] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for c-Myc.")
![Western Blot: TSG101 Antibody - Azide and BSA Free [TSG101] - Western blot analysis of lysates from K-562 cells using TSG101 Rabbit pAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 120s.](http://images.novusbio.com/fullsize/nbp2-94822_rabbit-polyclonal-tsg101-antibody-52202516321425.jpg "Western Blot: TSG101 Antibody - Azide and BSA Free [TSG101] - Western blot analysis of lysates from K-562 cells using TSG101 Rabbit pAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 120s.")
![<b>Genetic Strategies Validation. </b>Western Blot: TSG101 Antibody - Azide and BSA Free [TSG101] - Western blot analysis of lysates from wild type (WT) and TSG101 knockdown (KD) SH-SY5Y cells using TSG101 Rabbit pAb at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 120s.](http://images.novusbio.com/fullsize/nbp2-94822_rabbit-polyclonal-tsg101-antibody-52202516283277.jpg)
![Immunocytochemistry/ Immunofluorescence: TSG101 Antibody - Azide and BSA Free [TSG101] - Immunofluorescence analysis of PC-12 cells using TSG101 Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-94822_rabbit-polyclonal-tsg101-antibody-5220251638068.jpg)
![Immunocytochemistry/ Immunofluorescence: TSG101 Antibody - Azide and BSA Free [TSG101] - Immunofluorescence analysis of Jurkat cells using TSG101 Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-94822_rabbit-polyclonal-tsg101-antibody-5220251626465.jpg)
![Western Blot: TNFAIP1 Antibody [NBP2-82039] - Western blot analysis of TNFAIP1 in mouse brain tissue lysate with TNFAIP1 antibody at 1 ug/ml.](http://images.novusbio.com/fullsize/TNFAIP1-Antibody-Western-Blot-NBP2-82039-img0001.jpg "Western Blot: TNFAIP1 Antibody [NBP2-82039] - Western blot analysis of TNFAIP1 in mouse brain tissue lysate with TNFAIP1 antibody at 1 ug/ml.")
![Immunohistochemistry: TNFAIP1 Antibody - BSA Free [NBP2-82039] - Immunohistochemistry of TNFAIP1 in mouse brain tissue with TNFAIP1 antibody at 10 ug/ml.](http://images.novusbio.com/fullsize/nbp2-82039_rabbit-polyclonal-tnfaip1-antibody-271120241935386.jpg)
![Immunocytochemistry/ Immunofluorescence: TNFAIP1 Antibody - BSA Free [NBP2-82039] - Immunofluorescence of TNFAIP1 in mouse brain tissue with TNFAIP1 antibody at 20 ug/ml.Red: TNFAIP1 Antibody Blue: DAPI staining](http://images.novusbio.com/fullsize/nbp2-82039_rabbit-polyclonal-tnfaip1-antibody-2711202420285833.gif)
![Western Blot: ATG4C Antibody [NBP2-81954] - Analysis of ATG4C in A549 cell lysate with ATG4C antibody at 1 ug/ml.](http://images.novusbio.com/fullsize/ATG4C-Antibody-Western-Blot-NBP2-81954-img0001.jpg "Western Blot: ATG4C Antibody [NBP2-81954] - Analysis of ATG4C in A549 cell lysate with ATG4C antibody at 1 ug/ml.")
![Immunohistochemistry: ATG4C Antibody [NBP2-81954] - Analysis of ATG4C in human lung tissue with ATG4C antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/ATG4C-Antibody-Immunohistochemistry-NBP2-81954-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: ATG4C Antibody - BSA Free [NBP2-81954] - Immunofluorescence of ATG4C in human lung tissue with ATG4C antibody at 20 ug/ml.](http://images.novusbio.com/fullsize/nbp2-81954_rabbit-polyclonal-atg4c-antibody-271120242041457.jpg)
![Western Blot: TSG101 Antibody - Azide and BSA Free [NBP2-94900] - Western blot analysis of extracts from normal (control) and TSG101 knockdown (KD) SH-SY5Y(KD) cells, using TSG101 Rabbit pAb (A2216) at 1:800 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp2-94900_rabbit-polyclonal-tsg101-antibody-52202519294535.jpg "<b>Genetic Strategies Validation. </b>Western Blot: TSG101 Antibody - Azide and BSA Free [NBP2-94900] - Western blot analysis of extracts from normal (control) and TSG101 knockdown (KD) SH-SY5Y(KD) cells, using TSG101 Rabbit pAb (A2216) at 1:800 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 30s.")
![<b>Genetic Strategies Validation. </b>Western Blot: TSG101 Antibody - Azide and BSA Free [NBP2-94900] - Western blot analysis of K-562, using TSG101 Rabbit pAb (A2216) at 1:800 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp2-94900_rabbit-polyclonal-tsg101-antibody-52202519302972.jpg)
![Immunocytochemistry/ Immunofluorescence: TSG101 Antibody - Azide and BSA Free [NBP2-94900] - Immunofluorescence analysis of Jurkat cells using TSG101 Rabbit pAb (A2216) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-94900_rabbit-polyclonal-tsg101-antibody-52202519313476.jpg)
![Immunocytochemistry/ Immunofluorescence: TSG101 Antibody - Azide and BSA Free [NBP2-94900] - Immunofluorescence analysis of PC-12 cells using TSG101 Rabbit pAb (A2216) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-94900_rabbit-polyclonal-tsg101-antibody-522025193131.jpg)
![Western Blot: ATG101 Antibody [NBP2-81705] - Analysis of ATG101 in human liver tissue lysate with ATG101 antibody at (A) 1 and (B) 2 ug/ml.](http://images.novusbio.com/fullsize/ATG101-Antibody-Western-Blot-NBP2-81705-img0001.jpg "Western Blot: ATG101 Antibody [NBP2-81705] - Analysis of ATG101 in human liver tissue lysate with ATG101 antibody at (A) 1 and (B) 2 ug/ml.")
![Immunohistochemistry: ATG101 Antibody [NBP2-81705] - Immunohistochemistry of ATG101 in mouse liver with ATG101 antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/ATG101-Antibody-Immunohistochemistry-NBP2-81705-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: ATG101 Antibody - BSA Free [NBP2-81705] - Immunofluorescence of ATG101 in mouse liver tissue with ATG101 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp2-81705_rabbit-polyclonal-atg101-antibody-271120242074689.jpg)
![Western Blot: Beclin 1 Antibody (2A4) [NBP2-22176] - Analysis using Beclin 1 mouse mAb against Hela (1), A431 (2), MCF-7 (3), RAJI (4), Jurkat (5) and SKBR-3 (6) cell lysate.](http://images.novusbio.com/fullsize/Beclin-1-Antibody-2A4-Western-Blot-NBP2-22176-img0004.jpg "Western Blot: Beclin 1 Antibody (2A4) [NBP2-22176] - Analysis using Beclin 1 mouse mAb against Hela (1), A431 (2), MCF-7 (3), RAJI (4), Jurkat (5) and SKBR-3 (6) cell lysate.")
![Immunohistochemistry-Paraffin: Beclin 1 Antibody (2A4) [NBP2-22176] - Analysis of breast cancer tissues (left) and liver cancer tissues (right) using Beclin 1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Beclin-1-Antibody-2A4-Immunohistochemistry-Paraffin-NBP2-22176-img0002.jpg)
![Flow Cytometry: Beclin 1 Antibody (2A4) [NBP2-22176] - Analysis of RAJI cells using Beclin 1 mouse mAb (green) and negative control (purple).](http://images.novusbio.com/fullsize/Beclin-1-Antibody-2A4-Flow-Cytometry-NBP2-22176-img0001.jpg)
![ELISA: Beclin 1 Antibody (2A4) [NBP2-22176] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng)](http://images.novusbio.com/fullsize/Beclin-1-Antibody-2A4-ELISA-NBP2-22176-img0003.jpg)
![Western Blot: AMPK alpha 2 Antibody (5B4G1) [NBP2-61853] - Analysis using PRKAA2 mAb against human PRKAA2 (AA: 453-552) recombinant protein. (Expected MW is 36.7 kDa)](http://images.novusbio.com/fullsize/AMPK-alpha-2-Antibody-5B4G1-Western-Blot-NBP2-61853-img0004.jpg "Western Blot: AMPK alpha 2 Antibody (5B4G1) [NBP2-61853] - Analysis using PRKAA2 mAb against human PRKAA2 (AA: 453-552) recombinant protein. (Expected MW is 36.7 kDa)")
![Immunohistochemistry: AMPK alpha 2 Antibody (5B4G1) [NBP2-61853] - Analysis of paraffin-embedded rectum cancer tissues using PRKAA2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/AMPK-alpha-2-Antibody-5B4G1-Immunohistochemistry-NBP2-61853-img0003.jpg)
![Western Blot: AMPK alpha 2 Antibody (5B4G1) [NBP2-61853] - Analysis using PRKAA2 mAb against HEK293 (1) and PRKAA2 (AA: 453-552)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/AMPK-alpha-2-Antibody-5B4G1-Western-Blot-NBP2-61853-img0005.jpg)
![Immunohistochemistry: AMPK alpha 2 Antibody (5B4G1) [NBP2-61853] - Analysis of paraffin-embedded esophageal cancer tissues using PRKAA2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/AMPK-alpha-2-Antibody-5B4G1-Immunohistochemistry-NBP2-61853-img0002.jpg)
![ELISA: AMPK alpha 2 Antibody (5B4G1) [NBP2-61853] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/AMPK-alpha-2-Antibody-5B4G1-ELISA-NBP2-61853-img0001.jpg)
![Western Blot: PTEN Antibody (JE51-96) [NBP3-32873] - Western blot analysis of PTEN on different lysates with Rabbit anti-PTEN antibody (NBP3-32873) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: MCF7 cell lysate Lane 3: MDA-MB-468 cell lysate (negative) Lane 4: A431 cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: C6 cell lysate Lane 7: PC-12 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 47 kDa Observed band size: 55 kDa Exposure time: 2 minutes 18 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32873) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32873_rabbit-pten-mab-je51-96-288202412461629.jpg "Western Blot: PTEN Antibody (JE51-96) [NBP3-32873] - Western blot analysis of PTEN on different lysates with Rabbit anti-PTEN antibody (NBP3-32873) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: MCF7 cell lysate Lane 3: MDA-MB-468 cell lysate (negative) Lane 4: A431 cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: C6 cell lysate Lane 7: PC-12 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 47 kDa Observed band size: 55 kDa Exposure time: 2 minutes 18 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32873) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: PTEN Antibody (JE51-96) [NBP3-32873] - Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-PTEN antibody (NBP3-32873) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32873) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32873_rabbit-pten-mab-je51-96-29820241492644.jpg)
![Flow Cytometry: PTEN Antibody (JE51-96) [NBP3-32873] - Flow cytometric analysis of A431 cells labeling PTEN. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32873, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32873_rabbit-pten-mab-je51-96-29202416372137.jpg)
![Western Blot: APE Antibody (12H2-R) [NBP3-32043] - Western blot analysis of APE on different lysates with Mouse anti-APE antibody (NBP3-32043) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: LNCaP cell lysate (20 ug/Lane) Lane 3: HEK-293 cell lysate (20 ug/Lane) Lane 4: HL-60 cell lysate (20 ug/Lane) Lane 5: A431 cell lysate (20 ug/Lane) Lane 6: NIH/3T3 cell lysate (20 ug/Lane) Lane 7: C6 cell lysate (20 ug/Lane) Lane 8: Rat testis tissue lysate (40 ug/Lane) Predicted band size: 36 kDa Observed band size: 36 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32043) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32043_mouse-ape-mab-12h2-r-278202415212451.jpg "Western Blot: APE Antibody (12H2-R) [NBP3-32043] - Western blot analysis of APE on different lysates with Mouse anti-APE antibody (NBP3-32043) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: LNCaP cell lysate (20 ug/Lane) Lane 3: HEK-293 cell lysate (20 ug/Lane) Lane 4: HL-60 cell lysate (20 ug/Lane) Lane 5: A431 cell lysate (20 ug/Lane) Lane 6: NIH/3T3 cell lysate (20 ug/Lane) Lane 7: C6 cell lysate (20 ug/Lane) Lane 8: Rat testis tissue lysate (40 ug/Lane) Predicted band size: 36 kDa Observed band size: 36 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32043) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: APE Antibody (12H2-R) [NBP3-32043] - Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Mouse anti-APE antibody (NBP3-32043) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32043) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32043_mouse-ape-mab-12h2-r-298202414333696.jpg)
![Immunocytochemistry/ Immunofluorescence: APE Antibody (12H2-R) [NBP3-32043] - Immunocytochemistry analysis of A549 cells labeling APE with Mouse anti-APE antibody (NBP3-32043) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-APE antibody (NBP3-32043) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32043_mouse-ape-mab-12h2-r-2920241192367.jpg)
![Western Blot: HIF-1 alpha Antibody (JE75-33) [NBP3-32424] - Western blot analysis of HIF-1 alpha on different lysates with Rabbit anti-HIF-1 alpha antibody (NBP3-32424) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 250uM CoCl2 for 6 hours cell lysate Lane 3: HepG2 cell lysate Lane 4: HepG2 treated with 250uM CoCl2 for 6 hours cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 93 kDa Observed band size: 120 kDa Exposure time: 1minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32424) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32424_rabbit-hif-1-alpha-mab-je75-33-278202415212466.jpg "Western Blot: HIF-1 alpha Antibody (JE75-33) [NBP3-32424] - Western blot analysis of HIF-1 alpha on different lysates with Rabbit anti-HIF-1 alpha antibody (NBP3-32424) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 250uM CoCl2 for 6 hours cell lysate Lane 3: HepG2 cell lysate Lane 4: HepG2 treated with 250uM CoCl2 for 6 hours cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 93 kDa Observed band size: 120 kDa Exposure time: 1minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32424) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: HIF-1 alpha Antibody (JE75-33) [NBP3-32424] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-HIF-1 alpha antibody (NBP3-32424) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32424) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32424_rabbit-hif-1-alpha-mab-je75-33-2982024149260.jpg)
![Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody (JE75-33) [NBP3-32424] - Immunocytochemistry analysis of HeLa cells treated with or without 500μM CoCl2 for 24 hours labeling HIF-1 alpha with Rabbit anti-HIF-1 alpha antibody (NBP3-32424) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-HIF-1 alpha antibody (NBP3-32424) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32424_rabbit-hif-1-alpha-mab-je75-33-29202410334071.jpg)
![Western Blot: BNIP3L Antibody [NBP3-00002] - Anti-BNIP3L rabbit polyclonal antibody at 1:200 dilution. Lane A: K562 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 24 kDa. Observed band size: 37 kDa](http://images.novusbio.com/fullsize/BNIP3L-Antibody-Western-Blot-NBP3-00002-img0006.jpg "Western Blot: BNIP3L Antibody [NBP3-00002] - Anti-BNIP3L rabbit polyclonal antibody at 1:200 dilution. Lane A: K562 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 24 kDa. Observed band size: 37 kDa")
![Immunohistochemistry-Paraffin: BNIP3L Antibody [NBP3-00002] - Immunochemical staining of human BNIP3L in human kidney with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/BNIP3L-Antibody-Immunohistochemistry-Paraffin-NBP3-00002-img0004.jpg)
![Immunohistochemistry-Paraffin: BNIP3L Antibody [NBP3-00002] - Immunochemical staining of human BNIP3L in human breast carcinoma with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/BNIP3L-Antibody-Immunohistochemistry-Paraffin-NBP3-00002-img0003.jpg)
![Immunoprecipitation: BNIP3L Antibody [NBP3-00002] - GP-BNIP3L was immunoprecipitated using: Lane A: 0.5 mg K562 Whole Cell Lysate 4 ul anti-GP-BNIP3L rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-GP-BNIP3L rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 36 kDa. Observed band size: 36 kDa](http://images.novusbio.com/fullsize/BNIP3L-Antibody-Immunoprecipitation-NBP3-00002-img0005.jpg)