| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-27152-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-52655-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-75898SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56657SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-97416SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-44725 | |||
| Azide and BSA Free | NBP2-34427-0.1mg | |||
![ELISA: Human CD133 ELISA Kit (Colorimetric) [NBP3-38977] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-38977_human-cd133-elisa-kit-colorimetric-2110202415355096.png "ELISA: Human CD133 ELISA Kit (Colorimetric) [NBP3-38977] - Standard Curve Reference")
![Immunoprecipitation: PCNA Antibody (A6-G11-R) [NBP3-32725] - PCNA was immunoprecipitated from 0.2 mg HeLa cell lysate with NBP3-32725 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-32725 at 1/10,000 dilution. Anti-Mouse IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.<br/><br/> Lane 1: HeLa cell lysate (input)<br/> Lane 2: NBP3-32725 IP in HeLa cell lysate<br/> Lane 3: Mouse IgG instead of NBP3-32725 in HeLa cell lysate<br/><br/> Blocking/Dilution buffer: 5% NFDM/TBST<br/> Exposure time: 24 seconds; ECL](http://images.novusbio.com/fullsize/nbp3-32725_mouse-pcna-mab-a6-g11-r-2682024158269.jpg "Immunoprecipitation: PCNA Antibody (A6-G11-R) [NBP3-32725] - PCNA was immunoprecipitated from 0.2 mg HeLa cell lysate with NBP3-32725 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-32725 at 1/10,000 dilution. Anti-Mouse IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.<br/><br/> Lane 1: HeLa cell lysate (input)<br/> Lane 2: NBP3-32725 IP in HeLa cell lysate<br/> Lane 3: Mouse IgG instead of NBP3-32725 in HeLa cell lysate<br/><br/> Blocking/Dilution buffer: 5% NFDM/TBST<br/> Exposure time: 24 seconds; ECL")
![Immunohistochemistry: PCNA Antibody (A6-G11-R) [NBP3-32725] - Immunohistochemical analysis of paraffin-embedded human lymph node tissue with Mouse anti-PCNA antibody (NBP3-32725) at 1/2,000 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32725) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32725_mouse-pcna-mab-a6-g11-r-2982024149267.jpg)
![Immunocytochemistry/ Immunofluorescence: PCNA Antibody (A6-G11-R) [NBP3-32725] - Immunocytochemistry analysis of HeLa cells labeling PCNA with Mouse anti-PCNA antibody (NBP3-32725) at 1/100 dilution.<br/><br/> Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-PCNA antibody (NBP3-32725) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br/><br/> beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32725_mouse-pcna-mab-a6-g11-r-29202410484872.jpg)
![Immunohistochemistry: PRAS40 Antibody (JE30-60) [NBP3-32850] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-PRAS40 antibody (NBP3-32850) at 1/200 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32850) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32850_rabbit-pras40-mab-je30-60-298202413585526.jpg "Immunohistochemistry: PRAS40 Antibody (JE30-60) [NBP3-32850] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-PRAS40 antibody (NBP3-32850) at 1/200 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32850) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunocytochemistry/ Immunofluorescence: PRAS40 Antibody (JE30-60) [NBP3-32850] - Immunocytochemistry analysis of HeLa cells labeling PRAS40 with Rabbit anti-PRAS40 antibody (NBP3-32850) at 1/100 dilution.<br/><br/> Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-PRAS40 antibody (NBP3-32850) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br/><br/> Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32850_rabbit-pras40-mab-je30-60-2920241192362.jpg)
![Flow Cytometry: PRAS40 Antibody (JE30-60) [NBP3-32850] - Flow cytometric analysis of HeLa cells labeling PRAS40.<br/><br/> Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32850, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32850_rabbit-pras40-mab-je30-60-2920241637211.jpg)
![Western Blot: NF-L Antibody (PSH0-77) [NBP3-32649] - Western blot analysis of NF-L on different lysates with Rabbit anti-NF-L antibody (NBP3-32649) at 1/1,000 dilution.<br/><br/> Lane 1: Mouse brain tissue lysate<br/> Lane 2: Rat brain tissue lysate<br/> Lane 3: Mouse hippocampus tissue lysate<br/> Lane 4: Rat hippocampus tissue lysate<br/> Lane 5: Human liver tissue lysate (negative)<br/><br/> Lysates/proteins at 20 ug/Lane.<br/><br/> Predicted band size: 62 kDa<br/> Observed band size: 68 kDa<br/><br/> Exposure time: 1 minute 2 seconds;<br/><br/> 4-20% SDS-PAGE gel.<br/><br/> Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32649) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32649_rabbit-nf-l-mab-psh0-77-278202415212422.jpg "Western Blot: NF-L Antibody (PSH0-77) [NBP3-32649] - Western blot analysis of NF-L on different lysates with Rabbit anti-NF-L antibody (NBP3-32649) at 1/1,000 dilution.<br/><br/> Lane 1: Mouse brain tissue lysate<br/> Lane 2: Rat brain tissue lysate<br/> Lane 3: Mouse hippocampus tissue lysate<br/> Lane 4: Rat hippocampus tissue lysate<br/> Lane 5: Human liver tissue lysate (negative)<br/><br/> Lysates/proteins at 20 ug/Lane.<br/><br/> Predicted band size: 62 kDa<br/> Observed band size: 68 kDa<br/><br/> Exposure time: 1 minute 2 seconds;<br/><br/> 4-20% SDS-PAGE gel.<br/><br/> Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32649) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: NF-L Antibody (PSH0-77) [NBP3-32649] - Immunofluorescence analysis of paraffin-embedded mouse brain tissue labeling NF-L with Rabbit anti-NF-L antibody (NBP3-32649) at 1/200 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32649, green) at 1/200 dilution overnight at 4 ℃, washed with PBS.<br/><br/> Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32649_rabbit-nf-l-mab-psh0-77-29820241420992.jpg)
![Immunohistochemistry: NF-L Antibody (PSH0-77) [NBP3-32649] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-NF-L antibody (NBP3-32649) at 1/2,000 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32649) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32649_rabbit-nf-l-mab-psh0-77-298202414333659.jpg)
![Immunocytochemistry/ Immunofluorescence: NF-L Antibody (PSH0-77) [NBP3-32649] - Immunocytochemistry analysis of SH-SY5Y cells labeling NF-L with Rabbit anti-NF-L antibody (NBP3-32649) at 1/200 dilution.<br/><br/> Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-NF-L antibody (NBP3-32649) at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br/><br/> Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32649_rabbit-nf-l-mab-psh0-77-29202410234263.jpg)
![Immunohistochemistry: p70 S6 Kinase/S6K [p Thr389] Antibody (PSH02-23) [NBP3-32783] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-p70 S6 Kinase/S6K antibody (NBP3-32783) at 1/1,000 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32783) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32783_rabbit-p70-s6-kinase-s6k-p-thr389-mab-psh02-23-29820241492679.jpg "Immunohistochemistry: p70 S6 Kinase/S6K [p Thr389] Antibody (PSH02-23) [NBP3-32783] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-p70 S6 Kinase/S6K antibody (NBP3-32783) at 1/1,000 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32783) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunocytochemistry/ Immunofluorescence: p70 S6 Kinase/S6K [p Thr389] Antibody (PSH02-23) [NBP3-32783] - Immunocytochemistry analysis of MCF7 cells treated with or without 20% FBS overnight then add 100nM Calyculin A for 30 minutes labeling p70 S6 Kinase/S6K with Rabbit anti-p70 S6 Kinase/S6K antibody (NBP3-32783) at 1/100 dilution.<br/><br/> Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-p70 S6 Kinase/S6K antibody (NBP3-32783) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br/><br/> Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32783_rabbit-p70-s6-kinase-s6k-p-thr389-mab-psh02-23-292024111483.jpg)
![Western Blot: ATG9A Antibody (Atg9 14F2 8B1) [NBP2-50109] - Analysis of Hek293 lysates, non-transfected (nt) or transfected (trans) with HA-tagged human Atg9. Arrowhead indicates position of human Atg9 at 105 KDa.](http://images.novusbio.com/fullsize/ATG9A-Antibody-Atg9-14F2-8B1-Western-Blot-NBP2-50109-img0002.jpg "Western Blot: ATG9A Antibody (Atg9 14F2 8B1) [NBP2-50109] - Analysis of Hek293 lysates, non-transfected (nt) or transfected (trans) with HA-tagged human Atg9. Arrowhead indicates position of human Atg9 at 105 KDa.")
![Immunocytochemistry/Immunofluorescence: ATG9A Antibody (Atg9 14F2 8B1) [NBP2-50109] - Staining of PFA fixed HEK293 cells labelled with hamster anti-Atg9 (Left) and rabbit anti-Atg9.](http://images.novusbio.com/fullsize/ATG9A-Antibody-Atg9-14F2-8B1-Immunofluorescence-NBP2-50109-img0001.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: ATG9A Antibody (Atg9 14F2 8B1) [NBP2-50109] - Analysis of siRNA knockdown of human Atg9 (si) in Hela and Hek293 cells compared to siControl siRNA (siC) reveals specicity of hamster anti-Atg9 antibody.](http://images.novusbio.com/fullsize/ATG9A-Antibody-Atg9-14F2-8B1-Western-Blot-NBP2-50109-img0003.jpg)
![Western Blot: WDFY3 Antibody (2F7C2) [NBP3-27152] - Analysis using WDFY3 mAb against HEK293 (1) and WDFY3 (AA: 3277-3526)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/nbp3-27152_mouse-wdfy3-mab-2f7c2-bsa-free-10420249181942.jpg%3Fv=1666704387 "Western Blot: WDFY3 Antibody (2F7C2) [NBP3-27152] - Analysis using WDFY3 mAb against HEK293 (1) and WDFY3 (AA: 3277-3526)-hIgGFc transfected HEK293 (2) cell lysate.")
![Flow Cytometry: WDFY3 Antibody (2F7C2) [NBP3-27152] - Analysis of Hela cells using WDFY3 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/nbp3-27152_mouse-wdfy3-mab-2f7c2-bsa-free-104202491626.jpg%3Fv=1666704387)
![Immunohistochemistry: WDFY3 Antibody (2F7C2) [NBP3-27152] - Analysis of paraffin-embedded bladder cancer tissues using WDFY3 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/nbp3-27152_mouse-wdfy3-mab-2f7c2-bsa-free-1042024916227.jpg%3Fv=1666704387)
![ELISA: WDFY3 Antibody (2F7C2) [NBP3-27152] - Black line: Control Antigen (100 ng); Purple line: Antigen (10 ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)](http://images.novusbio.com/fullsize/nbp3-27152_mouse-wdfy3-mab-2f7c2-bsa-free-1042024920241.jpg%3Fv=1666704387)
![Western Blot: WDFY3 Antibody (2F7C2) [NBP3-27152] - Analysis using WDFY3 mAb against human WDFY3 (AA: 3277-3526) recombinant protein. (Expected MW is 54.2 kDa)](http://images.novusbio.com/fullsize/nbp3-27152_mouse-wdfy3-mab-2f7c2-bsa-free-104202491707.jpg%3Fv=1666704387)
![Western Blot: TNF-alpha Antibody (cA2 (Infliximab)) - Chimeric [NBP2-75898] - Mouse liver (lane 1), mouse spinal cord (lane 2), mouse testis (lane 3), mouse colon (lane 4) and human thyroid tumour (lane 5) samples were resolved on a 10% SDS PAGE gel and blots probed with NBP2-75898 at 1.5 ug/ml before being detected by a secondary antibody. The expected band size (269 kDa) is seen in the human-derived samples, as well as a band corresponding to dimers.](http://images.novusbio.com/fullsize/TNF-alpha-Antibody-cA2-Infliximab-Chimeric-Western-Blot-NBP2-75898-img0002.jpg "Western Blot: TNF-alpha Antibody (cA2 (Infliximab)) - Chimeric [NBP2-75898] - Mouse liver (lane 1), mouse spinal cord (lane 2), mouse testis (lane 3), mouse colon (lane 4) and human thyroid tumour (lane 5) samples were resolved on a 10% SDS PAGE gel and blots probed with NBP2-75898 at 1.5 ug/ml before being detected by a secondary antibody. The expected band size (269 kDa) is seen in the human-derived samples, as well as a band corresponding to dimers.")
![Western Blot: MDC1 Antibody [NB100-56657] - Analysis of MDC1 in 30 ugs of Jurkat cell lysate using this antibody at 1:1000 dilution.](http://images.novusbio.com/fullsize/MDC1-Antibody-Western-Blot-NB100-56657-img0002.jpg "Western Blot: MDC1 Antibody [NB100-56657] - Analysis of MDC1 in 30 ugs of Jurkat cell lysate using this antibody at 1:1000 dilution.")
![Immunohistochemistry: GABARAP Antibody [NBP1-97416] - IHC analysis of GABARAP in mouse liver using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/GABARAP-Antibody-Immunohistochemistry-NBP1-97416-img0001.jpg "Immunohistochemistry: GABARAP Antibody [NBP1-97416] - IHC analysis of GABARAP in mouse liver using DAB with hematoxylin counterstain.")
![Western Blot: NF-H Antibody (SPM563) [NBP2-34380] - Western Blot Analysis of human brain tissue lysate using NF-H MAb (SPM563).](http://images.novusbio.com/fullsize/NF-H-Antibody-SPM563-Western-Blot-NBP2-34380-img0002.jpg "Western Blot: NF-H Antibody (SPM563) [NBP2-34380] - Western Blot Analysis of human brain tissue lysate using NF-H MAb (SPM563).")
![Immunohistochemistry-Paraffin: NF-H Antibody (SPM563) [NBP2-34380] - Formalin-fixed, paraffin-embedded human cerebellum stained with Neurofilament Monoclonal Antibody (SPM563)](http://images.novusbio.com/fullsize/NF-H-Antibody-SPM563-Immunohistochemistry-Paraffin-NBP2-34380-img0001.jpg)