| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-97502-0.2mg | |||
| BSA Free | NBP1-97502SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-36567SS | |||
| Azide and BSA Free | NBP2-80653 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-71049 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-71309 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-72647 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier-Free | NBP2-34889-5ug | |||
| Carrier-Free | NBP2-34889-1000ug | |||
| Carrier-Free | NBP2-34889-500ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56100SS | |||
![Western Blot: Calreticulin Antibody (FMC 75) [NBP1-97502] - Analysis of Calreticulin: Lane 1: HeLa (Heat Shocked), Lane 2: Vero.](http://images.novusbio.com/fullsize/Calreticulin-Antibody-FMC-75-Western-Blot-NBP1-97502-img0002.jpg "Western Blot: Calreticulin Antibody (FMC 75) [NBP1-97502] - Analysis of Calreticulin: Lane 1: HeLa (Heat Shocked), Lane 2: Vero.")
![Immunohistochemistry-Paraffin: Calreticulin Antibody (FMC 75) [NBP1-97502] - Analysis of human uterus tissue stained with Calreticulin, mAb (FMC 75) at 10ug/ml.](http://images.novusbio.com/fullsize/Calreticulin-Antibody-FMC-75-Immunohistochemistry-Paraffin-NBP1-97502-img0001.jpg)
![Western Blot: CD63 Antibody (7G4.2E8) [NBP2-36567] - Western blot analysis of mouse CD63 on recombinant mouse CD63 and mouse kidney lysate.](http://images.novusbio.com/fullsize/CD63-Antibody-7G4-2E8-Western-Blot-NBP2-36567-img0002.jpg "Western Blot: CD63 Antibody (7G4.2E8) [NBP2-36567] - Western blot analysis of mouse CD63 on recombinant mouse CD63 and mouse kidney lysate.")
![Flow Cytometry: CD63 Antibody (7G4.2E8) [NBP2-36567] - Intracellular staining of mouse CD63 in Flow cytometry using 2.0 ug of antibody per 1 million cells. Isotype control was rat IgG2a lambda.](http://images.novusbio.com/fullsize/CD63-Antibody-7G4-2E8-Flow-Cytometry-NBP2-36567-img0003.jpg)
![SDS-Page: Recombinant Human TNF-alpha Protein [NBC1-18460] - 15% SDS-PAGE (3ug)](http://images.novusbio.com/fullsize/Recombinant-Human-TNF-alpha-Protein-SDS-Page-NBC1-18460-img0002.jpg "SDS-Page: Recombinant Human TNF-alpha Protein [NBC1-18460] - 15% SDS-PAGE (3ug)")
![Western Blot: IRS1 Antibody (3G10) [NBP2-45435] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IRS1.](http://images.novusbio.com/fullsize/IRS1-Antibody-3G10-Western-Blot-NBP2-45435-img0018.jpg "Western Blot: IRS1 Antibody (3G10) [NBP2-45435] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IRS1.")
![Immunohistochemistry: IRS1 Antibody (3G10) [NBP2-45435] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/IRS1-Antibody-3G10-Immunohistochemistry-NBP2-45435-img0017.jpg)
![Immunohistochemistry: IRS1 Antibody (3G10) [NBP2-45435] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/IRS1-Antibody-3G10-Immunohistochemistry-NBP2-45435-img0001.jpg)
![Immunohistochemistry: IRS1 Antibody (3G10) [NBP2-45435] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/IRS1-Antibody-3G10-Immunohistochemistry-NBP2-45435-img0002.jpg)
![Immunohistochemistry: IRS1 Antibody (3G10) [NBP2-45435] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/IRS1-Antibody-3G10-Immunohistochemistry-NBP2-45435-img0003.jpg)
![Immunohistochemistry: IRS1 Antibody (3G10) [NBP2-45435] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/IRS1-Antibody-3G10-Immunohistochemistry-NBP2-45435-img0004.jpg)
![Immunohistochemistry: IRS1 Antibody (3G10) [NBP2-45435] - Analysis of Carcinoma of Human kidney tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/IRS1-Antibody-3G10-Immunohistochemistry-NBP2-45435-img0005.jpg)
![Immunohistochemistry: IRS1 Antibody (3G10) [NBP2-45435] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/IRS1-Antibody-3G10-Immunohistochemistry-NBP2-45435-img0006.jpg)
![Immunohistochemistry: IRS1 Antibody (3G10) [NBP2-45435] - Analysis of Carcinoma of Human liver tissue.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/IRS1-Antibody-3G10-Immunohistochemistry-NBP2-45435-img0007.jpg)
![Immunohistochemistry: IRS1 Antibody (3G10) [NBP2-45435] - Analysis of Human lung tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/IRS1-Antibody-3G10-Immunohistochemistry-NBP2-45435-img0008.jpg)
![Immunohistochemistry: IRS1 Antibody (3G10) [NBP2-45435] - Analysis of Human Ovary tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/IRS1-Antibody-3G10-Immunohistochemistry-NBP2-45435-img0009.jpg)
![Immunohistochemistry: IRS1 Antibody (3G10) [NBP2-45435] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/IRS1-Antibody-3G10-Immunohistochemistry-NBP2-45435-img0010.jpg)
![Immunohistochemistry: IRS1 Antibody (3G10) [NBP2-45435] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/IRS1-Antibody-3G10-Immunohistochemistry-NBP2-45435-img0011.jpg)
![Immunohistochemistry: IRS1 Antibody (3G10) [NBP2-45435] - Analysis of Human thyroid tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/IRS1-Antibody-3G10-Immunohistochemistry-NBP2-45435-img0012.jpg)
![Immunohistochemistry: IRS1 Antibody (3G10) [NBP2-45435] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/IRS1-Antibody-3G10-Immunohistochemistry-NBP2-45435-img0013.jpg)
![Immunohistochemistry: IRS1 Antibody (3G10) [NBP2-45435] - Analysis of Human endometrium tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/IRS1-Antibody-3G10-Immunohistochemistry-NBP2-45435-img0014.jpg)
![Immunohistochemistry: IRS1 Antibody (3G10) [NBP2-45435] - Analysis of Human bladder tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/IRS1-Antibody-3G10-Immunohistochemistry-NBP2-45435-img0015.jpg)
![Immunohistochemistry: IRS1 Antibody (3G10) [NBP2-45435] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/IRS1-Antibody-3G10-Immunohistochemistry-NBP2-45435-img0016.jpg)
![Western Blot: Endothelial Lipase Antibody (OTI1D9) [NBP2-02396] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Endothelial Lipase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Endothelial Lipase.](http://images.novusbio.com/fullsize/Endothelial-Lipase-Antibody-OTI1D9-Western-Blot-NBP2-02396-img0001.jpg "Western Blot: Endothelial Lipase Antibody (OTI1D9) [NBP2-02396] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Endothelial Lipase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Endothelial Lipase.")
![Immunocytochemistry/Immunofluorescence: Endothelial Lipase Antibody (OTI1D9) [NBP2-02396] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Endothelial Lipase.](http://images.novusbio.com/fullsize/Endothelial-Lipase-Antibody-OTI1D9-Immunocytochemistry-Immunofluorescence-NBP2-02396-img0008.jpg)
![Immunohistochemistry-Paraffin: Endothelial Lipase Antibody (OTI1D9) [NBP2-02396] - Staining of paraffin-embedded Human liver tissue using anti-Endothelial Lipase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Endothelial-Lipase-Antibody-OTI1D9-Immunohistochemistry-Paraffin-NBP2-02396-img0007.jpg)
![Flow Cytometry: Endothelial Lipase Antibody (OTI1D9) [NBP2-02396] - HEK293T cells transfected with either Endothelial Lipase Human Tagged ORF Clone overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-LIPG antibody , and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/Endothelial-Lipase-Antibody-OTI1D9-Flow-Cytometry-NBP2-02396-img0010.jpg)
![Immunohistochemistry-Paraffin: Endothelial Lipase Antibody (OTI1D9) [NBP2-02396] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-Endothelial Lipase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Endothelial-Lipase-Antibody-OTI1D9-Immunohistochemistry-Paraffin-NBP2-02396-img0002.jpg)
![Immunohistochemistry-Paraffin: Endothelial Lipase Antibody (OTI1D9) [NBP2-02396] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-Endothelial Lipase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Endothelial-Lipase-Antibody-OTI1D9-Immunohistochemistry-Paraffin-NBP2-02396-img0003.jpg)
![Immunohistochemistry-Paraffin: Endothelial Lipase Antibody (OTI1D9) [NBP2-02396] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-Endothelial Lipase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Endothelial-Lipase-Antibody-OTI1D9-Immunohistochemistry-Paraffin-NBP2-02396-img0004.jpg)
![Immunohistochemistry-Paraffin: Endothelial Lipase Antibody (OTI1D9) [NBP2-02396] - Staining of paraffin-embedded Human breast tissue using anti-Endothelial Lipase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Endothelial-Lipase-Antibody-OTI1D9-Immunohistochemistry-Paraffin-NBP2-02396-img0005.jpg)
![Immunohistochemistry-Paraffin: Endothelial Lipase Antibody (OTI1D9) [NBP2-02396] - Staining of paraffin-embedded Human Kidney tissue using anti-Endothelial Lipase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Endothelial-Lipase-Antibody-OTI1D9-Immunohistochemistry-Paraffin-NBP2-02396-img0006.jpg)
![Immunoprecipitation: Endothelial Lipase Antibody (OTI1D9) [NBP2-02396] - Lane 1: Negative Control Lane 2: NBP2-02378 Lane 3: NBP2-02380 Lane 4:NBP2-02381 Lane 5: NBP2-02382 Lane 6: NBP2-02394 Lane 7: NBP2-02395 Lane 8: NBP2-02396 Lane 9: NBP2-02397](http://images.novusbio.com/fullsize/Endothelial-Lipase-Antibody-OTI1D9-Immunoprecipitation-NBP2-02396-img0009.jpg)
![Western Blot: MEK2 Antibody (OTI1A2) [NBP2-45802] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/MEK2-Antibody-OTI1A2-Western-Blot-NBP2-45802-img0008.jpg "Western Blot: MEK2 Antibody (OTI1A2) [NBP2-45802] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).")
![Immunohistochemistry: MEK2 Antibody (OTI1A2) [NBP2-45802] - Analysis of Carcinoma of Human lung tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/MEK2-Antibody-OTI1A2-Immunohistochemistry-NBP2-45802-img0007.jpg)
![Western Blot: MEK2 Antibody (OTI1A2) [NBP2-45802] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MEK2.](http://images.novusbio.com/fullsize/MEK2-Antibody-OTI1A2-Western-Blot-NBP2-45802-img0009.jpg)
![Immunohistochemistry: MEK2 Antibody (OTI1A2) [NBP2-45802] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/MEK2-Antibody-OTI1A2-Immunohistochemistry-NBP2-45802-img0002.jpg)
![Immunohistochemistry: MEK2 Antibody (OTI1A2) [NBP2-45802] - Analysis of Carcinoma of Human liver tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/MEK2-Antibody-OTI1A2-Immunohistochemistry-NBP2-45802-img0003.jpg)
![Immunohistochemistry: MEK2 Antibody (OTI1A2) [NBP2-45802] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/MEK2-Antibody-OTI1A2-Immunohistochemistry-NBP2-45802-img0004.jpg)
![Immunohistochemistry: MEK2 Antibody (OTI1A2) [NBP2-45802] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/MEK2-Antibody-OTI1A2-Immunohistochemistry-NBP2-45802-img0005.jpg)
![Immunohistochemistry: MEK2 Antibody (OTI1A2) [NBP2-45802] - Analysis of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/MEK2-Antibody-OTI1A2-Immunohistochemistry-NBP2-45802-img0006.jpg)
![Immunofluorescence: MEK2 Antibody (OTI1A2) [NBP2-45802] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY MEK2.](http://images.novusbio.com/fullsize/MEK2-Antibody-OTI1A2-Immunofluorescence-NBP2-45802-img0001.jpg)
![SDS-Page: Human Bcl-2 Protein [NBP2-34889]](http://images.novusbio.com/fullsize/Human-Bcl-2-Protein-SDS-Page-NBP2-34889-img0001.jpg "SDS-Page: Human Bcl-2 Protein [NBP2-34889]")
![Western Blot: VPS35 Antibody (7E4) [NBP2-78823] - Western Blot analysis of Human SH-SY5Y lysates showing detection of 91.7 kDa VPS35 protein using Mouse Anti-VPS35 Monoclonal Antibody, Clone 7E4 (NBP2-78823). Lane 1: Molecular Weight Ladder. Lane 2: SH-SY5Y. Load: 10 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody (NBP2-78823) at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Predicted/Observed Size: 91.7 kDa.](http://images.novusbio.com/fullsize/VPS35-Antibody-7E4-Western-Blot-NBP2-78823-img0013.jpg "Western Blot: VPS35 Antibody (7E4) [NBP2-78823] - Western Blot analysis of Human SH-SY5Y lysates showing detection of 91.7 kDa VPS35 protein using Mouse Anti-VPS35 Monoclonal Antibody, Clone 7E4 (NBP2-78823). Lane 1: Molecular Weight Ladder. Lane 2: SH-SY5Y. Load: 10 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody (NBP2-78823) at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Predicted/Observed Size: 91.7 kDa.")
![Western Blot: VPS35 Antibody (7E4) [NBP2-78823] - Western Blot analysis of Human, Mouse A549, MEF showing detection of VPS35 protein using Mouse Anti-VPS35 Monoclonal Antibody, Clone 7E4 (NBP2-78823). Lane 1: Molecular Weight Ladder. Lane 2: VPS35 KO A549 cells. Lane 3: mouse embryonic fibroblast cells.. Load: 8 ug each A549 and MEF. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody (NBP2-78823) at 1:5 (tissue culture supernatant). Secondary Antibody: Donkey anti-mouse IRDye 800CW at 1:25000 in TBS-T.](http://images.novusbio.com/fullsize/VPS35-Antibody-7E4-Western-Blot-NBP2-78823-img0012.jpg)
![Immunoprecipitation: VPS35 Antibody (7E4) [NBP2-78823] - Tissue: embryonic fibroblast. Species: Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody (NBP2-78823). Three amounts of NBP2-78823 (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4 degrees C and next incubated with 250ug of MEF lysate for 2 hours at 4 degrees C.](http://images.novusbio.com/fullsize/VPS35-Antibody-7E4-Immunoprecipitation-NBP2-78823-img0005.jpg)
![Immunoprecipitation: VPS35 Antibody (7E4) [NBP2-78823] - Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 7E4 . Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. 500 uL cell culture supernatants were incubated with 10 uL of Protein A/G resin beads for 1 hour at 4C. Clone 7E4 depletes virtually all of the VPS35 from the A549 cell extract.](http://images.novusbio.com/fullsize/VPS35-Antibody-7E4-Immunoprecipitation-NBP2-78823-img0008.jpg)
![Immunoprecipitation: VPS35 Antibody (7E4) [NBP2-78823] - Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 7E4. Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody.](http://images.novusbio.com/fullsize/VPS35-Antibody-7E4-Immunoprecipitation-NBP2-78823-img0009.jpg)
![Immunoprecipitation: VPS35 Antibody (7E4) [NBP2-78823] - Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 7E4. Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts of NBP2-78823 (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4C and next incubated with 250ug of A549 lysate for 2 hours at 4C.](http://images.novusbio.com/fullsize/VPS35-Antibody-7E4-Immunoprecipitation-NBP2-78823-img0010.jpg)
![Immunoprecipitation: VPS35 Antibody (7E4) [NBP2-78823] - Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 7E4. Tissue: MEF, A549 cells. Species: Human, Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody (SMC-602) at 1:5 (tissue culture supernatant). 10 ug antibody were coupled to 10 uL A/G resin beads either covalently (with DMP) or non-covalently (1 hour at 4 degrees). The antibody immunoprecipitates VPS35 in mouse and human cells effectively when covalently coupled to the beads..](http://images.novusbio.com/fullsize/VPS35-Antibody-7E4-Immunoprecipitation-NBP2-78823-img0011.jpg)
![Western Blot: VPS35 Antibody (8A3) [NBP2-78825] - Western Blot analysis of Human SH-SY5Y showing detection of VPS35 protein using Mouse Anti-VPS35 Monoclonal Antibody, Clone 8A3 (NBP2-78825). Lane 1: Molecular Weight Ladder. Lane 2: SH-SY5Y (10 ug). Load: 10 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody (NBP2-78825) at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT.](http://images.novusbio.com/fullsize/VPS35-Antibody-8A3-Western-Blot-NBP2-78825-img0012.jpg "Western Blot: VPS35 Antibody (8A3) [NBP2-78825] - Western Blot analysis of Human SH-SY5Y showing detection of VPS35 protein using Mouse Anti-VPS35 Monoclonal Antibody, Clone 8A3 (NBP2-78825). Lane 1: Molecular Weight Ladder. Lane 2: SH-SY5Y (10 ug). Load: 10 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody (NBP2-78825) at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT.")
![Immunohistochemistry: VPS35 Antibody (8A3) [NBP2-78825] - Immunohistochemistry analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 8A3 (NBP2-78825). Tissue: Kidney. Species: Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody (NBP2-78825) at 1:100 for Overnight at 4C, then 30 min at 37C. Secondary Antibody: Goat Anti-Mouse IgG (H+L): FITC for 45 min at 37C. Counterstain: DAPI for 3 min at RT. Magnification: 20X.](http://images.novusbio.com/fullsize/VPS35-Antibody-8A3-Immunohistochemistry-NBP2-78825-img0013.jpg)
![Western Blot: VPS35 Antibody (8A3) [NBP2-78825] - Western Blot analysis of Human, Mouse A549, MEF showing detection of VPS35 protein using Mouse Anti-VPS35 Monoclonal Antibody, Clone 8A3 (NBP2-78825). Lane 1: Molecular Weight Ladder. Lane 2: VPS35 KO A549 cells. Lane 3: mouse embryonic fibroblast cells.. Load: 8 ug each A549 and MEF. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody (NBP2-78825) at 1:5 (tissue culture supernatant). Secondary Antibody: Donkey anti-mouse IRDye 800CW at 1:25000 in TBS-T.](http://images.novusbio.com/fullsize/VPS35-Antibody-8A3-Western-Blot-NBP2-78825-img0011.jpg)
![Immunoprecipitation: VPS35 Antibody (8A3) [NBP2-78825] - Tissue: embryonic fibroblast. Species: Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody (NBP2-78825). Three amounts of NBP2-78825 (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4 degrees C and next incubated with 250ug of MEF lysate for 2 hours at 4 degrees C.](http://images.novusbio.com/fullsize/VPS35-Antibody-8A3-Immunoprecipitation-NBP2-78825-img0001.jpg)
![Immunoprecipitation: VPS35 Antibody (8A3) [NBP2-78825] - Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 8A3. Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. 500 uL cell culture supernatants were incubated with 10 uL of Protein A/G resin beads for 1 hour at 4C. clone 8A3 depletes VPS35 from the A549 cell extract.](http://images.novusbio.com/fullsize/VPS35-Antibody-8A3-Immunoprecipitation-NBP2-78825-img0008.jpg)
![Immunoprecipitation: VPS35 Antibody (8A3) [NBP2-78825] - Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 8A3. Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody.](http://images.novusbio.com/fullsize/VPS35-Antibody-8A3-Immunoprecipitation-NBP2-78825-img0009.jpg)
![Immunoprecipitation: VPS35 Antibody (8A3) [NBP2-78825] - Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 8A3. Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts of NBP2-78825 (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4C and next incubated with 250ug of A549 lysate for 2 hours at 4C.](http://images.novusbio.com/fullsize/VPS35-Antibody-8A3-Immunoprecipitation-NBP2-78825-img0010.jpg)
![Immunohistochemistry-Paraffin: Bcl-2 Antibody [NB100-56100] - FFPE section from a secondary follicle of the mouse lymph node stained for Bcl-2 expression using NB100-56100 at 1:2000. A, Abundant staining is seen in the mantle zone, and little staining is seen in the germinal center. (A1) is a higher magnification of (A).](http://images.novusbio.com/fullsize/Bcl-2-Antibody-Immunohistochemistry-Paraffin-NB100-56100-img0002.jpg "Immunohistochemistry-Paraffin: Bcl-2 Antibody [NB100-56100] - FFPE section from a secondary follicle of the mouse lymph node stained for Bcl-2 expression using NB100-56100 at 1:2000. A, Abundant staining is seen in the mantle zone, and little staining is seen in the germinal center. (A1) is a higher magnification of (A).")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: UVRAG Antibody [NBP1-18885] - Section of human colon carcinoma. Antibody: Affinity purified rabbit anti- UVRAG used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/UVRAG-Antibody-Immunohistochemistry-Paraffin-NBP1-18885-img0004.jpg)
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: UVRAG Antibody [NBP1-18885] - Whole cell lysate from HeLa and 293T cells. UVRAG was also immunoprecipitated by rabbit anti-UVRAG antibody NBP1-18884.](http://images.novusbio.com/fullsize/UVRAG-Antibody-Western-Blot-NBP1-18885-img0003.jpg)
![Chromatin Immunoprecipitation: GAPDH primer [NBP1-71650] - 2 ug of NB21-1023 was used to IP DNA from fixed Hela cells alongside a no antibody (No Ab) control. DNA was measured by qRT-PCR and normalized to total input (input=1).](http://images.novusbio.com/fullsize/GAPDH-primer-Chromatin-Immunoprecipitation-NBP1-71650-img0004.jpg "Chromatin Immunoprecipitation: GAPDH primer [NBP1-71650] - 2 ug of NB21-1023 was used to IP DNA from fixed Hela cells alongside a no antibody (No Ab) control. DNA was measured by qRT-PCR and normalized to total input (input=1).")
![Chromatin Immunoprecipitation: GAPDH primer [NBP1-71650] - qRT-PCR standard curve generated from a serial dilution of sheared Hela chromatin.](http://images.novusbio.com/fullsize/GAPDH-primer-Chromatin-Immunoprecipitation-NBP1-71650-img0003.jpg)