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![SDS-Page: Recombinant Human VPS35 Protein [H00055737-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/Recombinant-Human-VPS35-Protein-SDS-Page-H00055737-P01-img0001.jpg "SDS-Page: Recombinant Human VPS35 Protein [H00055737-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Western Blot: p70 S6 Kinase beta/S6K2 Antibody (4B11) [H00006199-M08] - Analysis of RPS6KB2 expression in transfected 293T cell line by RPS6KB2 monoclonal antibody (M08), clone 4B11. Lane 1: RPS6KB2 transfected lysatE (53.4 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/p70-S6-Kinase-beta-S6K2-Antibody-4B11-Western-Blot-H00006199-M08-img0005.jpg "Western Blot: p70 S6 Kinase beta/S6K2 Antibody (4B11) [H00006199-M08] - Analysis of RPS6KB2 expression in transfected 293T cell line by RPS6KB2 monoclonal antibody (M08), clone 4B11. Lane 1: RPS6KB2 transfected lysatE (53.4 KDa). Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: p70 S6 Kinase beta/S6K2 Antibody (4B11) [H00006199-M08] - Analysis of monoclonal antibody to RPS6KB2 on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/p70-S6-Kinase-beta-S6K2-Antibody-4B11-Immunocytochemistry-Immunofluorescence-H00006199-M08-img0004.jpg)
![ELISA: p70 S6 Kinase beta/S6K2 Antibody (4B11) [H00006199-M08] - Detection limit for recombinant GST tagged RPS6KB2 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/p70-S6-Kinase-beta-S6K2-Antibody-4B11-ELISA-H00006199-M08-img0002.jpg)
![Western Blot: RPTOR Antibody (JE59-39) [NBP3-32894] - Western blot analysis of RPTOR on different lysates with Rabbit anti-RPTOR antibody (NBP3-32894) at 1/2,000 dilution. Lane 1: 293T cell lysate (30 ug/Lane) Lane 2: K-562 cell lysate (30 ug/Lane) Lane 3: Neuro-2a cell lysate (30 ug/Lane) Lane 4: Mouse brain tissue lysate (40 ug/Lane) Lane 5: Rat brain tissue lysate (40 ug/Lane) Predicted band size: 149 kDa Observed band size: 149 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32894) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32894_rabbit-rptor-mab-je59-39-288202412442987.jpg "Western Blot: RPTOR Antibody (JE59-39) [NBP3-32894] - Western blot analysis of RPTOR on different lysates with Rabbit anti-RPTOR antibody (NBP3-32894) at 1/2,000 dilution. Lane 1: 293T cell lysate (30 ug/Lane) Lane 2: K-562 cell lysate (30 ug/Lane) Lane 3: Neuro-2a cell lysate (30 ug/Lane) Lane 4: Mouse brain tissue lysate (40 ug/Lane) Lane 5: Rat brain tissue lysate (40 ug/Lane) Predicted band size: 149 kDa Observed band size: 149 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32894) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: RPTOR Antibody (JE59-39) [NBP3-32894] - Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-RPTOR antibody (NBP3-32894) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32894) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32894_rabbit-rptor-mab-je59-39-29820241435299.jpg)
![Western Blot: ATG12 Antibody (JE30-61) [NBP3-32056] - Western blot analysis of ATG12 on different lysates with Rabbit anti-ATG12 antibody (NBP3-32056) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HCT 116 cell lysate Lane 3: HEK-293 cell lysate Lane 4: SH-SY5Y cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 15 kDa Observed band size: 55/20 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32056) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32056_rabbit-atg12-mab-je30-61-278202416285257.jpg "Western Blot: ATG12 Antibody (JE30-61) [NBP3-32056] - Western blot analysis of ATG12 on different lysates with Rabbit anti-ATG12 antibody (NBP3-32056) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HCT 116 cell lysate Lane 3: HEK-293 cell lysate Lane 4: SH-SY5Y cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 15 kDa Observed band size: 55/20 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32056) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: ATG12 Antibody (JE30-61) [NBP3-32056] - Immunocytochemistry analysis of HCT 116 cells labeling ATG12 with Rabbit anti-ATG12 antibody (NBP3-32056) at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-ATG12 antibody (NBP3-32056) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32056_rabbit-atg12-mab-je30-61-2920241192327.jpg)
![Flow Cytometry: ATG12 Antibody (JE30-61) [NBP3-32056] - Flow cytometric analysis of HCT 116 cells labeling ATG12. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32056, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32056_rabbit-atg12-mab-je30-61-29202416453286.jpg)
![Western Blot: Endothelial Lipase Antibody [NBP1-45541] - Analysis of Endothelial Lipase with Jurkat cell lysate.](http://images.novusbio.com/fullsize/Endothelial-Lipase-Antibody-Western-Blot-NBP1-45541-img0002.jpg "Western Blot: Endothelial Lipase Antibody [NBP1-45541] - Analysis of Endothelial Lipase with Jurkat cell lysate.")
![Immunohistochemistry-Paraffin: c-Myc Antibody (MYC275) - Azide and BSA Free [NBP2-34691] - Formalin-fixed, paraffin-embedded human Cervical carcinoma stained with c-Myc Monoclonal Antibody (MYC275).](http://images.novusbio.com/fullsize/c-Myc-Antibody-MYC275-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-34691-img0001.jpg "Immunohistochemistry-Paraffin: c-Myc Antibody (MYC275) - Azide and BSA Free [NBP2-34691] - Formalin-fixed, paraffin-embedded human Cervical carcinoma stained with c-Myc Monoclonal Antibody (MYC275).")
