| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61715-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB600-956-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56100SS | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Biotin | NBP2-21679 | |||
| DyLight 488 | NBP1-77702 | |||
![Western Blot: p70 S6 Kinase beta/S6K2 Antibody (5D8F5) [NBP3-16752] - Western blot analysis of extracts of Mouse brain, using p70 S6 Kinase beta/S6K2 Rabbit mAb (NBP3-16752) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.](http://images.novusbio.com/fullsize/p70-S6-Kinase-beta-S6K2-Antibody-5D8F5-Western-Blot-NBP3-16752-img0006.jpg "Western Blot: p70 S6 Kinase beta/S6K2 Antibody (5D8F5) [NBP3-16752] - Western blot analysis of extracts of Mouse brain, using p70 S6 Kinase beta/S6K2 Rabbit mAb (NBP3-16752) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.")
![Immunocytochemistry/ Immunofluorescence: p70 S6 Kinase beta/S6K2 Antibody (5D8F5) [NBP3-16752] - Confocal imaging of C2C12 cells using p70 S6 Kinase beta/S6K2 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16752_rabbit-p70-s6-kinase-beta-s6k2-mab-5d8f5-822025209833.jpg)
![Immunocytochemistry/ Immunofluorescence: p70 S6 Kinase beta/S6K2 Antibody (5D8F5) [NBP3-16752] - Confocal imaging of NIH/3T3 cells using p70 S6 Kinase beta/S6K2 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16752_rabbit-p70-s6-kinase-beta-s6k2-mab-5d8f5-822025208493.jpg)
![Immunocytochemistry/ Immunofluorescence: p70 S6 Kinase beta/S6K2 Antibody (5D8F5) [NBP3-16752] - Confocal imaging of U-2 OS cells using p70 S6 Kinase beta/S6K2 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16752_rabbit-p70-s6-kinase-beta-s6k2-mab-5d8f5-822025209869.jpg)
![Immunohistochemistry: p70 S6 Kinase beta/S6K2 Antibody (5D8F5) [NBP3-16752] - Immunohistochemistry analysis of p70 S6 Kinase beta/S6K2 in paraffin-embedded human cervix cancer tissue using p70 S6 Kinase beta/S6K2 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16752_rabbit-p70-s6-kinase-beta-s6k2-mab-5d8f5-8220252010494.jpg)
![Immunohistochemistry: p70 S6 Kinase beta/S6K2 Antibody (5D8F5) [NBP3-16752] - Immunohistochemistry analysis of p70 S6 Kinase beta/S6K2 in paraffin-embedded mouse kidney tissue using p70 S6 Kinase beta/S6K2 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16752_rabbit-p70-s6-kinase-beta-s6k2-mab-5d8f5-8220252010403.jpg)
![Immunohistochemistry: p70 S6 Kinase beta/S6K2 Antibody (5D8F5) [NBP3-16752] - Immunohistochemistry analysis of p70 S6 Kinase beta/S6K2 in paraffin-embedded human colon carcinoma tissue using p70 S6 Kinase beta/S6K2 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16752_rabbit-p70-s6-kinase-beta-s6k2-mab-5d8f5-8220252010481.jpg)
![Immunohistochemistry: p70 S6 Kinase beta/S6K2 Antibody (5D8F5) [NBP3-16752] - Immunohistochemistry analysis of p70 S6 Kinase beta/S6K2 in paraffin-embedded rat colon tissue using p70 S6 Kinase beta/S6K2 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16752_rabbit-p70-s6-kinase-beta-s6k2-mab-5d8f5-822025208423.jpg)
![Western Blot: Huntingtin Antibody (7B9C8) [NBP3-15642] - Western blot analysis of extracts of various cell lines, using Huntingtin antibody (NBP3-15642) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Huntingtin-Antibody-7B9C8-Western-Blot-NBP3-15642-img0005.jpg "Western Blot: Huntingtin Antibody (7B9C8) [NBP3-15642] - Western blot analysis of extracts of various cell lines, using Huntingtin antibody (NBP3-15642) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunohistochemistry-Paraffin: Huntingtin Antibody (7B9C8) [NBP3-15642] - Immunohistochemistry of paraffin-embedded mouse spinal cord using Huntingtin Rabbit mAb (NBP3-15642) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Huntingtin-Antibody-7B9C8-Immunohistochemistry-Paraffin-NBP3-15642-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: Huntingtin Antibody (7B9C8) [Huntingtin] - Immunofluorescence analysis of Mouse brain tissue using Huntingtin Rabbit mAb at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. Microwave antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-15642_rabbit-huntingtin-mab-7b9c8-52202517344224.jpg)
![Western Blot: Huntingtin Antibody (7B9C8) [Huntingtin] - Western blot analysis of various lysates using Huntingtin Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-15642_rabbit-huntingtin-mab-7b9c8-52202517333895.jpg)
![Immunohistochemistry: Huntingtin Antibody (7B9C8) [Huntingtin] - Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using Huntingtin Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15642_rabbit-huntingtin-mab-7b9c8-52202517304947.jpg)
![Immunohistochemistry: Huntingtin Antibody (7B9C8) [Huntingtin] - Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using Huntingtin Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15642_rabbit-huntingtin-mab-7b9c8-52202517315522.jpg)
![Immunocytochemistry/ Immunofluorescence: Huntingtin Antibody (7B9C8) [Huntingtin] - Immunofluorescence analysis of Rat brain tissue using Huntingtin Rabbit mAb at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. Microwave antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-15642_rabbit-huntingtin-mab-7b9c8-52202517361214.jpg)
![Immunohistochemistry: Huntingtin Antibody (7B9C8) [Huntingtin] - Immunohistochemistry analysis of paraffin-embedded Human brain tissue using Huntingtin Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15642_rabbit-huntingtin-mab-7b9c8-52202517333868.jpg)
![Western Blot: Huntingtin Antibody (7B9C8) [Huntingtin] - Western blot analysis of various lysates using Huntingtin Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 3min.](http://images.novusbio.com/fullsize/nbp3-15642_rabbit-huntingtin-mab-7b9c8-5220251734569.jpg)
![Western Blot: BNIP3 Antibody (6A5F7) [NBP2-61715] - Analysis using BNIP3 mAb against human BNIP3 (AA: 50-155) recombinant protein. (Expected MW is 38 kDa)](http://images.novusbio.com/fullsize/BNIP3-Antibody-6A5F7-Western-Blot-NBP2-61715-img0006.jpg "Western Blot: BNIP3 Antibody (6A5F7) [NBP2-61715] - Analysis using BNIP3 mAb against human BNIP3 (AA: 50-155) recombinant protein. (Expected MW is 38 kDa)")
![Immunocytochemistry/Immunofluorescence: BNIP3 Antibody (6A5F7) [NBP2-61715] - Analysis of Hela cells using BNIP3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize/BNIP3-Antibody-6A5F7-Immunocytochemistry-Immunofluorescence-NBP2-61715-img0003.jpg)
![Immunohistochemistry-Paraffin: BNIP3 Antibody (6A5F7) [NBP2-61715] - Analysis of rectum cancer tissues using BNIP3 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/BNIP3-Antibody-6A5F7-Immunohistochemistry-NBP2-61715-img0005.jpg)
![Flow Cytometry: BNIP3 Antibody (6A5F7) [NBP2-61715] - Analysis of Hela cells using BNIP3 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/BNIP3-Antibody-6A5F7-Flow-Cytometry-NBP2-61715-img0002.jpg)
![Western Blot: BNIP3 Antibody (6A5F7) [NBP2-61715] - Analysis using BNIP3 mAb against HEK293 (1) and BNIP3 (AA: 50-155)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/BNIP3-Antibody-6A5F7-Western-Blot-NBP2-61715-img0007.jpg)
![Immunohistochemistry-Paraffin: BNIP3 Antibody (6A5F7) [NBP2-61715] - Analysis of breast cancer tissues using BNIP3 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/BNIP3-Antibody-6A5F7-Immunohistochemistry-NBP2-61715-img0004.jpg)
![ELISA: BNIP3 Antibody (6A5F7) [NBP2-61715] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/BNIP3-Antibody-6A5F7-ELISA-NBP2-61715-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: LAMP-1/CD107a Antibody - BSA Free [NB600-956] - Human HeLa cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.4% Saponin. Fixed cells were stained with 5 ug/mL Anti-LAMP1 and developed with Goat Anti-Rabbit IgG, Cy3 conjugate.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NB600-956-img0006.jpg "Immunocytochemistry/Immunofluorescence: LAMP-1/CD107a Antibody - BSA Free [NB600-956] - Human HeLa cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.4% Saponin. Fixed cells were stained with 5 ug/mL Anti-LAMP1 and developed with Goat Anti-Rabbit IgG, Cy3 conjugate.")
![Immunocytochemistry/Immunofluorescence: LAMP-1/CD107a Antibody - BSA Free [NB600-956] - Rat NRK cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.4% Saponin. Fixed cells were stained with 10 ug/mL Anti-LAMP1 and developed with Goat Anti-Rabbit IgG, FITC conjugate.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NB600-956-img0005.jpg)
![Western Blot: USP9x Antibody (JG35-11) [NBP2-75704] - Analysis of USP9X on different lysates using anti-USP9X antibody at 1/1,000 dilution.Positive control:Lane 1: SiHa Lane 2: A549Lane 3: 293 Lane 4: Mouse colon](http://images.novusbio.com/fullsize/USP9x-Antibody-JG35-11-Western-Blot-NBP2-75704-img0007.jpg "Western Blot: USP9x Antibody (JG35-11) [NBP2-75704] - Analysis of USP9X on different lysates using anti-USP9X antibody at 1/1,000 dilution.Positive control:Lane 1: SiHa Lane 2: A549Lane 3: 293 Lane 4: Mouse colon")
![Immunocytochemistry/Immunofluorescence: USP9x Antibody (JG35-11) [NBP2-75704] - Staining USP9X in F9 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/USP9x-Antibody-JG35-11-Immunocytochemistry-Immunofluorescence-NBP2-75704-img0004.jpg)
![Immunohistochemistry-Paraffin: USP9x Antibody (JG35-11) [NBP2-75704] - Analysis of paraffin-embedded mouse fallopian tube tissue using anti-USP9X antibody. Counter stained with hematoxylin. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.](http://images.novusbio.com/fullsize/USP9x-Antibody-JG35-11-Immunohistochemistry-Paraffin-NBP2-75704-img0006.jpg)
![Flow Cytometry: USP9x Antibody (JG35-11) [NBP2-75704] - Analysis of 293T cells with USP9X antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow).Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/USP9x-Antibody-JG35-11-Flow-Cytometry-NBP2-75704-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: USP9x Antibody (JG35-11) [NBP2-75704] - Staining USP9X in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/USP9x-Antibody-JG35-11-Immunocytochemistry-Immunofluorescence-NBP2-75704-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: USP9x Antibody (JG35-11) [NBP2-75704] - Staining USP9X in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/USP9x-Antibody-JG35-11-Immunocytochemistry-Immunofluorescence-NBP2-75704-img0003.jpg)
![Immunohistochemistry-Paraffin: USP9x Antibody (JG35-11) [NBP2-75704] - Analysis of paraffin-embedded human tonsil tissue using anti-USP9X antibody. Counter stained with hematoxylin. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.](http://images.novusbio.com/fullsize/USP9x-Antibody-JG35-11-Immunohistochemistry-Paraffin-NBP2-75704-img0005.jpg)
![Western Blot: NF-M Antibody (JM11-20) [NBP2-66978] - Analysis of NEFM on 293T cells lysates using anti-NEFM antibody at 1/500 dilution.](http://images.novusbio.com/fullsize/NF-M-Antibody-JM11-20-Western-Blot-NBP2-66978-img0003.jpg "Western Blot: NF-M Antibody (JM11-20) [NBP2-66978] - Analysis of NEFM on 293T cells lysates using anti-NEFM antibody at 1/500 dilution.")
![Immunocytochemistry/Immunofluorescence: NF-M Antibody (JM11-20) [NBP2-66978] - Staining NEFM in PC-12 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/NF-M-Antibody-JM11-20-Immunocytochemistry-Immunofluorescence-NBP2-66978-img0006.jpg)
![Immunohistochemistry-Paraffin: NF-M Antibody (JM11-20) [NBP2-66978] - Analysis of paraffin-embedded human brain tissue with Rabbit anti-NF-M antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/NF-M-Antibody-JM11-20-Immunohistochemistry-Paraffin-NBP2-66978-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: NF-M Antibody (JM11-20) [NBP2-66978] - Staining NEFM in SH-SY5Y cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/NF-M-Antibody-JM11-20-Immunocytochemistry-Immunofluorescence-NBP2-66978-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: NF-M Antibody (JM11-20) [NBP2-66978] - Staining NEFM in N2A cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/NF-M-Antibody-JM11-20-Immunocytochemistry-Immunofluorescence-NBP2-66978-img0005.jpg)
![Immunohistochemistry-Paraffin: NF-M Antibody (JM11-20) [NBP2-66978] - Analysis of paraffin-embedded mouse brain tissue using anti-NEFM antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/NF-M-Antibody-JM11-20-Immunohistochemistry-Paraffin-NBP2-66978-img0001.jpg)
![Immunohistochemistry-Paraffin: NF-M Antibody (JM11-20) [NBP2-66978] - Analysis of paraffin-embedded rat brain tissue using anti-NEFM antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/NF-M-Antibody-JM11-20-Immunohistochemistry-Paraffin-NBP2-66978-img0002.jpg)
![Western Blot: BNIP3 Antibody (JA71-10) [NBP2-67192] - Analysis of BNIP3 on mouse kidney tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/BNIP3-Antibody-JA71-10-Western-Blot-NBP2-67192-img0009.jpg "Western Blot: BNIP3 Antibody (JA71-10) [NBP2-67192] - Analysis of BNIP3 on mouse kidney tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: BNIP3 Antibody (JA71-10) [NBP2-67192] - Staining BNIP3 in NIH-3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/BNIP3-Antibody-JA71-10-Immunocytochemistry-Immunofluorescence-NBP2-67192-img0008.jpg)
![Immunohistochemistry-Paraffin: BNIP3 Antibody (JA71-10) [NBP2-67192] - Analysis of paraffin-embedded mouse brain tissue using anti-BNIP3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/BNIP3-Antibody-JA71-10-Immunohistochemistry-Paraffin-NBP2-67192-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: BNIP3 Antibody (JA71-10) [NBP2-67192] - Staining BNIP3 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/BNIP3-Antibody-JA71-10-Immunocytochemistry-Immunofluorescence-NBP2-67192-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: BNIP3 Antibody (JA71-10) [NBP2-67192] - Staining BNIP3 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/BNIP3-Antibody-JA71-10-Immunocytochemistry-Immunofluorescence-NBP2-67192-img0007.jpg)
![Immunohistochemistry-Paraffin: BNIP3 Antibody (JA71-10) [NBP2-67192] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-BNIP3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/BNIP3-Antibody-JA71-10-Immunohistochemistry-Paraffin-NBP2-67192-img0002.jpg)
![Immunohistochemistry-Paraffin: BNIP3 Antibody (JA71-10) [NBP2-67192] - Analysis of paraffin-embedded human colon cancer tissue using anti-BNIP3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/BNIP3-Antibody-JA71-10-Immunohistochemistry-Paraffin-NBP2-67192-img0003.jpg)
![Immunohistochemistry-Paraffin: BNIP3 Antibody (JA71-10) [NBP2-67192] - Analysis of paraffin-embedded human kidney tissue using anti-BNIP3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/BNIP3-Antibody-JA71-10-Immunohistochemistry-Paraffin-NBP2-67192-img0004.jpg)
![Western Blot: VPS35 Antibody (8A3) [NBP2-78825] - Western Blot analysis of Human SH-SY5Y showing detection of VPS35 protein using Mouse Anti-VPS35 Monoclonal Antibody, Clone 8A3 (NBP2-78825). Lane 1: Molecular Weight Ladder. Lane 2: SH-SY5Y (10 ug). Load: 10 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody (NBP2-78825) at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT.](http://images.novusbio.com/fullsize/VPS35-Antibody-8A3-Western-Blot-NBP2-78825-img0012.jpg "Western Blot: VPS35 Antibody (8A3) [NBP2-78825] - Western Blot analysis of Human SH-SY5Y showing detection of VPS35 protein using Mouse Anti-VPS35 Monoclonal Antibody, Clone 8A3 (NBP2-78825). Lane 1: Molecular Weight Ladder. Lane 2: SH-SY5Y (10 ug). Load: 10 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody (NBP2-78825) at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT.")
![Immunohistochemistry: VPS35 Antibody (8A3) [NBP2-78825] - Immunohistochemistry analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 8A3 (NBP2-78825). Tissue: Kidney. Species: Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody (NBP2-78825) at 1:100 for Overnight at 4C, then 30 min at 37C. Secondary Antibody: Goat Anti-Mouse IgG (H+L): FITC for 45 min at 37C. Counterstain: DAPI for 3 min at RT. Magnification: 20X.](http://images.novusbio.com/fullsize/VPS35-Antibody-8A3-Immunohistochemistry-NBP2-78825-img0013.jpg)
![Western Blot: VPS35 Antibody (8A3) [NBP2-78825] - Western Blot analysis of Human, Mouse A549, MEF showing detection of VPS35 protein using Mouse Anti-VPS35 Monoclonal Antibody, Clone 8A3 (NBP2-78825). Lane 1: Molecular Weight Ladder. Lane 2: VPS35 KO A549 cells. Lane 3: mouse embryonic fibroblast cells.. Load: 8 ug each A549 and MEF. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody (NBP2-78825) at 1:5 (tissue culture supernatant). Secondary Antibody: Donkey anti-mouse IRDye 800CW at 1:25000 in TBS-T.](http://images.novusbio.com/fullsize/VPS35-Antibody-8A3-Western-Blot-NBP2-78825-img0011.jpg)
![Immunoprecipitation: VPS35 Antibody (8A3) [NBP2-78825] - Tissue: embryonic fibroblast. Species: Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody (NBP2-78825). Three amounts of NBP2-78825 (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4 degrees C and next incubated with 250ug of MEF lysate for 2 hours at 4 degrees C.](http://images.novusbio.com/fullsize/VPS35-Antibody-8A3-Immunoprecipitation-NBP2-78825-img0001.jpg)
![Immunoprecipitation: VPS35 Antibody (8A3) [NBP2-78825] - Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 8A3. Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. 500 uL cell culture supernatants were incubated with 10 uL of Protein A/G resin beads for 1 hour at 4C. clone 8A3 depletes VPS35 from the A549 cell extract.](http://images.novusbio.com/fullsize/VPS35-Antibody-8A3-Immunoprecipitation-NBP2-78825-img0008.jpg)
![Immunoprecipitation: VPS35 Antibody (8A3) [NBP2-78825] - Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 8A3. Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody.](http://images.novusbio.com/fullsize/VPS35-Antibody-8A3-Immunoprecipitation-NBP2-78825-img0009.jpg)
![Immunoprecipitation: VPS35 Antibody (8A3) [NBP2-78825] - Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 8A3. Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts of NBP2-78825 (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4C and next incubated with 250ug of A549 lysate for 2 hours at 4C.](http://images.novusbio.com/fullsize/VPS35-Antibody-8A3-Immunoprecipitation-NBP2-78825-img0010.jpg)
![Western Blot: HeLa Whole Cell Lysate [NB800-PC1] - WB analysis of HeLa whole cell lysate (NB800-PC1) using p14ARF antibody (NB200-111) with ECL detection method.](http://images.novusbio.com/fullsize/HeLa-Whole-Cell-Lysate-Western-Blot-NB800-PC1-img0007.jpg "Western Blot: HeLa Whole Cell Lysate [NB800-PC1] - WB analysis of HeLa whole cell lysate (NB800-PC1) using p14ARF antibody (NB200-111) with ECL detection method.")
![Western Blot: HeLa Whole Cell Lysate [NB800-PC1] - Ponceau S staining of HeLa whole cell lysate using NB800-PC1.](http://images.novusbio.com/fullsize/HeLa-Whole-Cell-Lysate-Western-Blot-NB800-PC1-img0003.jpg)
![Western Blot: HeLa Whole Cell Lysate [NB800-PC1] - Analysis of STIM-1 antibody (NB110-60547) using HeLa whole cell lysate [NB800-PC1].](http://images.novusbio.com/fullsize/HeLa-Whole-Cell-Lysate-Western-Blot-NB800-PC1-img0004.jpg)
![Western Blot: HeLa Whole Cell Lysate [NB800-PC1] - WB analysis of 30ug of HeLa whole cell lysate (NB800-PC1) using rabbit polyclonal Survivin antibody (NB 500-201) at 1ug/ml concentration. Detection was performed using ECL based method with 1 minute exposure time.](http://images.novusbio.com/fullsize/HeLa-Whole-Cell-Lysate-Western-Blot-NB800-PC1-img0005.jpg)
![Western Blot: HeLa Whole Cell Lysate [NB800-PC1] - WB analysis of HeLa whole cell lysate (NB800-PC1) using rabbit polyclonal NBS1 antibody (NB100-143) with detection employing ECL method.](http://images.novusbio.com/fullsize/HeLa-Whole-Cell-Lysate-Western-Blot-NB800-PC1-img0006.jpg)
![Western Blot: MEK1 Antibody [NBP2-17265] - Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC 10% SDS PAGE gel, diluted at 1:2000.](http://images.novusbio.com/fullsize/MEK1-Antibody-Western-Blot-NBP2-17265-img0002.jpg "Western Blot: MEK1 Antibody [NBP2-17265] - Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC 10% SDS PAGE gel, diluted at 1:2000.")
![Immunohistochemistry-Paraffin: MEK1 Antibody [NBP2-17265] - Paraffin-embedded rat liver. MEK1+2 antibody diluted at 1:500.](http://images.novusbio.com/fullsize/MEK1-Antibody-Immunohistochemistry-Paraffin-NBP2-17265-img0003.jpg)
![Immunocytochemistry/ Immunofluorescence: MEK1 Antibody [NBP2-17265] - MEK1 antibody detects MEK1 protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: MDCK cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: MEK1 stained by MEK1 antibody (NBP2-17265) diluted at 1:500.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp2-17265_rabbit-polyclonal-mek1-antibody-910202420134045.jpg)
![Western Blot: MEK1 Antibody [NBP2-17265] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with MEK1 antibody (NBP2-17265) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-17265_rabbit-polyclonal-mek1-antibody-9102024202464.jpg)
![Western Blot: MEK1 Antibody [NBP2-17265] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with MEK1 antibody (NBP2-17265) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp2-17265_rabbit-polyclonal-mek1-antibody-910202420134035.jpg)
![Immunohistochemistry-Paraffin: Bcl-2 Antibody [NB100-56100] - FFPE section from a secondary follicle of the mouse lymph node stained for Bcl-2 expression using NB100-56100 at 1:2000. A, Abundant staining is seen in the mantle zone, and little staining is seen in the germinal center. (A1) is a higher magnification of (A).](http://images.novusbio.com/fullsize/Bcl-2-Antibody-Immunohistochemistry-Paraffin-NB100-56100-img0002.jpg "Immunohistochemistry-Paraffin: Bcl-2 Antibody [NB100-56100] - FFPE section from a secondary follicle of the mouse lymph node stained for Bcl-2 expression using NB100-56100 at 1:2000. A, Abundant staining is seen in the mantle zone, and little staining is seen in the germinal center. (A1) is a higher magnification of (A).")
![Western Blot: AKT1 [p Thr308] Antibody (18F3.H11) [NBP1-69924] - Lane 1: non-phosphorylated AKT in untreated cells. Lane 2: phosphorylated AKT on PDGF stimulated NIH/3T3 cell lysates. Load: 15 ug per lane. Primary antibody: AKT phospho Thr308 antibody at a 1:4000 dilution in TBS with 3% BSA, for 3 h at 4 C. Secondary antibody: peroxidase conjugated Gt-a-Mouse IgG (Fc) at a 1:40,000 dilution for 1 h at 4 C. Block: 3% BSA in TBS for 30 min at RT. Predicted/Observed size: (indicated by arrowhead at 56 kDa). Other band(s): unspecific.](http://images.novusbio.com/fullsize/AKT1-p-Thr308-Antibody-18F3-H11-Western-Blot-NBP1-69924-img0011.jpg "Western Blot: AKT1 [p Thr308] Antibody (18F3.H11) [NBP1-69924] - Lane 1: non-phosphorylated AKT in untreated cells. Lane 2: phosphorylated AKT on PDGF stimulated NIH/3T3 cell lysates. Load: 15 ug per lane. Primary antibody: AKT phospho Thr308 antibody at a 1:4000 dilution in TBS with 3% BSA, for 3 h at 4 C. Secondary antibody: peroxidase conjugated Gt-a-Mouse IgG (Fc) at a 1:40,000 dilution for 1 h at 4 C. Block: 3% BSA in TBS for 30 min at RT. Predicted/Observed size: (indicated by arrowhead at 56 kDa). Other band(s): unspecific.")
![Immunocytochemistry/Immunofluorescence: AKT1 [p Thr308] Antibody (18F3.H11) [NBP1-69924] - IHC-P analysis of FFPE human prostate tissue using AKT1 phospho Thr308 antibody at 20 ug/mL. Left panel: 20X magnification. Right panel: 40X magnification. Heat antigen retrieval in citrate buffer pH 6.2. Secondary antibody: streptavidin-HRP at 10 ug/mL. Antibody as precipitated red signal with hemotoxylin purple nuclear counterstain. Image using the Biotin format of this antibody.](http://images.novusbio.com/fullsize/AKT1-p-Thr308-Antibody-18F3-H11-Immunocytochemistry-Immunofluorescence-NBP1-69924-img0014.jpg)
![Immunohistochemistry-Paraffin: AKT1 [p Thr308] Antibody (18F3.H11) [NBP1-69924] - IHC analysis. Detection of phosphorylated AKT phospho Thr308 in human FFPE brain cerebellum tissue (40X). AKT1 phospho Thr308 antibody was used at 20 ug/mL. The image shows strong staining of Purkinje neurons. Red: AKT1 pT308. Purple: Hematoxylin nuclear counterstain.](http://images.novusbio.com/fullsize/AKT1-p-Thr308-Antibody-18F3-H11-Immunohistochemistry-Paraffin-NBP1-69924-img0003.jpg)
![Western Blot: AKT1 [p Thr308] Antibody (18F3.H11) [NBP1-69924] - WB analysis using the Biotin conjugate of AKT1 pT308 antibody. Detection of Lane 1: GST tagged AKT1 active recombinant protein. Lane 2: GST tagged AKT1 inactive recombinant protein. Load: 25 ng per lane. AKT pT308 Biotin conjugated antibody at 1:1000 overnight at 4C. Secondary antibody: HRP Streptavidin.](http://images.novusbio.com/fullsize/AKT1-p-Thr308-Antibody-18F3-H11-Western-Blot-NBP1-69924-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: AKT1 [p Thr308] Antibody (18F3.H11) [NBP1-69924] - ICC/IF analysis using the DyLight 488 conjugate of AKT1 pT308, NBP1-69924. Histone detection using DyLight 488 conjugate (green). Anti-Tubulin was detected using DyLight 549 conjugate (red). Nuclei were counter-stained using DAPI (blue).](http://images.novusbio.com/fullsize/AKT1-p-Thr308-Antibody-18F3-H11-Immunocytochemistry-Immunofluorescence-NBP1-69924-img0006.jpg)
![ELISA: AKT1 [p Thr308] Antibody (18F3.H11) [NBP1-69924] - ELISA of AKT1 phospho Thr308 Biotin conjugated antibody. Antigen: Unconjugated AKT1 phospho Thr308 and AKT1 non-phospho Thr308. Coating amount: 0.1 ug per well. Primary antibody: AKT1 phospho Thr308 Biotin conjugated antibody at 5 ug/mL. Dilution series: 3-fold. Mid-point concentration: 5 ng/mL AKT1 phospho Thr308 Biotin conjugated antibody. Secondary antibody: Peroxidase streptavidin secondary antibody at 1:10,000. Substrate: TMB](http://images.novusbio.com/fullsize/AKT1-p-Thr308-Antibody-18F3-H11-ELISA-NBP1-69924-img0013.jpg)
