| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87753-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-38524-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70146 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-72646 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-73271 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-37822-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB600-956-0.025ml | |||
![Western Blot: CD63 Antibody (SY21-02) [NBP2-67425] - Analysis of CD63 on different lysates using anti-CD63 antibody at 1/1,000 dilution. Positive control: Lane 1: HL60 Lane 2: THP-1 Lane 3: NIH/3T3](http://images.novusbio.com/fullsize/CD63-Antibody-SY21-02-Western-Blot-NBP2-67425-img0003.jpg "Western Blot: CD63 Antibody (SY21-02) [NBP2-67425] - Analysis of CD63 on different lysates using anti-CD63 antibody at 1/1,000 dilution. Positive control: Lane 1: HL60 Lane 2: THP-1 Lane 3: NIH/3T3")
![Immunohistochemistry-Paraffin: CD63 Antibody (SY21-02) [NBP2-67425] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD63 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1607-2, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/CD63-Antibody-SY21-02-Immunohistochemistry-Paraffin-NBP2-67425-img0004.jpg)
![Immunohistochemistry-Paraffin: CD63 Antibody (SY21-02) [NBP2-67425] - Analysis of paraffin-embedded human lung cancer tissue using anti-CD63 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CD63-Antibody-SY21-02-Immunohistochemistry-Paraffin-NBP2-67425-img0001.jpg)
![Immunohistochemistry-Paraffin: CD63 Antibody (SY21-02) [NBP2-67425] - Analysis of paraffin-embedded human tonsil tissue using anti-CD63 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CD63-Antibody-SY21-02-Immunohistochemistry-Paraffin-NBP2-67425-img0002.jpg)
![Immunohistochemistry-Paraffin: NF-M Antibody [NBP1-87753] - Analysis in human cerebral cortex and endometrium tissues using NBP1-87753 antibody. Corresponding NEFM RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/NF-M-Antibody-Immunohistochemistry-Paraffin-NBP1-87753-img0014.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: NF-M Antibody [NBP1-87753] - Analysis in human cerebral cortex and endometrium tissues using NBP1-87753 antibody. Corresponding NEFM RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: NF-M Antibody [NBP1-87753] - Staining of human cerebellum shows moderate cytoplasmic positivity in Purkinje cell.](http://images.novusbio.com/fullsize/NF-M-Antibody-Immunohistochemistry-NBP1-87753-img0003.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: NF-M Antibody [NBP1-87753] - Analysis NBP1-87753 (A) shows similar pattern to independent antibody NBP1-87754 (B).](http://images.novusbio.com/fullsize/NF-M-Antibody-Western-Blot-NBP1-87753-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: NF-M Antibody [NBP1-87753] - Staining of human cell line HEK 293 shows localization to intermediate filaments. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/NF-M-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87753-img0011.jpg)
![Immunohistochemistry-Paraffin: NF-M Antibody [NBP1-87753] - Staining of human endometrium shows no positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/NF-M-Antibody-Immunohistochemistry-Paraffin-NBP1-87753-img0018.jpg)
![Western Blot: NF-M Antibody [NBP1-87753] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10 Lane 2: Mouse Cerebral Cortex tissue](http://images.novusbio.com/fullsize/NF-M-Antibody-Western-Blot-NBP1-87753-img0006.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: NF-M Antibody [NBP1-87753] - Analysis in human cell line HEK 293 and human cell line A-431.](http://images.novusbio.com/fullsize/NF-M-Antibody-Western-Blot-NBP1-87753-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: NF-M Antibody [NBP1-87753] - Staining of human cell line A-431 shows positivity in cytoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/NF-M-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87753-img0004.jpg)
![Immunohistochemistry-Paraffin: NF-M Antibody [NBP1-87753] - Staining of human cerebral cortex shows strong positivity in neuronal processes.](http://images.novusbio.com/fullsize/NF-M-Antibody-Immunohistochemistry-Paraffin-NBP1-87753-img0015.jpg)
![Immunohistochemistry-Paraffin: NF-M Antibody [NBP1-87753] - Staining of human prostate shows strong positivity in peripheral nerve.](http://images.novusbio.com/fullsize/NF-M-Antibody-Immunohistochemistry-Paraffin-NBP1-87753-img0017.jpg)
![Immunohistochemistry-Paraffin: NF-M Antibody [NBP1-87753] - Staining of human tonsil shows no positivity in lymphoid cells as expected.](http://images.novusbio.com/fullsize/NF-M-Antibody-Immunohistochemistry-Paraffin-NBP1-87753-img0019.jpg)
![Western Blot: ATG10 Antibody [NBP2-38524] - ATG10 is involved in p62-regulated autophagy. RPE cells were transfected with p62 or scrambled siRNA for 24h and then exposed with 400 H2O2 for 1, 3, 6, 12h respectively. Whole-cells lysates were analyzed by Western blot with anti-p62, ATG10, or beta-actin antibodies. Differences between means were considered statistically significant when p<0.05 by Mann-Whitney U-test. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0171940), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ATG10-Antibody-Western-Blot-NBP2-38524-img0006.jpg "<b>Biological Strategies Validation. </b>Western Blot: ATG10 Antibody [NBP2-38524] - ATG10 is involved in p62-regulated autophagy. RPE cells were transfected with p62 or scrambled siRNA for 24h and then exposed with 400 H2O2 for 1, 3, 6, 12h respectively. Whole-cells lysates were analyzed by Western blot with anti-p62, ATG10, or beta-actin antibodies. Differences between means were considered statistically significant when p<0.05 by Mann-Whitney U-test. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0171940), licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: ATG10 Antibody [NBP2-38524] - Immunofluorescent staining of human cell line MCF7 shows localization to nucleus & nucleoli. Antibody staining shown in green.](http://images.novusbio.com/fullsize/ATG10-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38524-img0004.jpg)
![Immunohistochemistry-Paraffin: ATG10 Antibody [NBP2-38524] - Staining of human stomach shows moderate membranous and cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/ATG10-Antibody-Immunohistochemistry-Paraffin-NBP2-38524-img0005.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: ATG10 Antibody [NBP2-38524] - ARPE-19 cells were transfected with pooled ATG10 specific siRNAs or scrambled siRNA (30nM) for 24h. ATG10 and ATG5 levels were assessed in protein lysates by Western Blot. (B) RNA isolated from cells was analyzed for ATG10 mRNA expression by qRT- PCR. Differences between means were considered statistically significant when p<0.05 by Mann-Whitney U-test. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28222108/), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/nbp2-38524_rabbit-polyclonal-atg10-antibody-20920231412576.jpg)
![Western Blot: ATG10 Antibody [NBP2-38524] - Knockdown of ATG10 makes ARPE-19 cells more sensitive to H2O2.(A) ARPE-19 cells were transfected with pooled ATG10 specific siRNAs or scrambled siRNA (30nM) for 24h. ATG10 & ATG5 levels were assessed in protein lysates by Western Blot. (B) RNA isolated from cells was analyzed for ATG10 mRNA expression by qRT- PCR. Differences between means were considered statistically significant when p<0.05 by Mann-Whitney U-test. (C) Lysates of ARPE-19 cells transfected with siRNAs for 24 hours were assessed for p62 & LC3 levels by Western blot. One set of wells per experiment was exposed to Bafilomycin A1 (75nM) for 3h prior to harvest. beta -actin was used as an internal control. ‘Short’ & ‘Long’ Exposure indicate time of exposure of immunoblot image. (D) 24h after transfection with respective siRNAs, ARPE-19 were subjected to oxidative stress with 800μM H2O2 & incubated at 37°C for 6h. Total cell numbers were determined by trypsinization & counting on TC10® cell counter. (E) Primary fetal RPE cells were transfected with scrambled or pooled ATG10 specific siRNAs & 48 hours after transfection, were subjected to H2O2 for 24 hours & MTT assay was performed. Differences between means were considered statistically significant when p<0.05 by Student’s t-test. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28222108), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-38524_rabbit-polyclonal-atg10-antibody-31020241613645.jpg)
![Western Blot: VPS35 Antibody (HL1017) [NBP3-13680] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS35 antibody [HL1017] (NBP3-13680) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/VPS35-Antibody-HL1017-Western-Blot-NBP3-13680-img0006.jpg "<b>Genetic Strategies Validation. </b>Western Blot: VPS35 Antibody (HL1017) [NBP3-13680] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS35 antibody [HL1017] (NBP3-13680) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: VPS35 Antibody (HL1017) [NBP3-13680] - VPS35 antibody detects VPS35 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: VPS35 stained by VPS35 antibody (NBP3-13680) diluted at 1:500.](http://images.novusbio.com/fullsize/VPS35-Antibody-HL1017-Immunocytochemistry-Immunofluorescence-NBP3-13680-img0004.jpg)
![Immunohistochemistry-Paraffin: VPS35 Antibody (HL1017) [NBP3-13680] - VPS35 antibody [HL1017] detects VPS35 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. VPS35 stained by VPS35 antibody [HL1017] (NBP3-13680) diluted at 1:50. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/VPS35-Antibody-HL1017-Immunohistochemistry-Paraffin-NBP3-13680-img0005.jpg)
![Western Blot: VPS35 Antibody (HL1017) [NBP3-13680] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS35 antibody [HL1017] (NBP3-13680) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/VPS35-Antibody-HL1017-Western-Blot-NBP3-13680-img0001.jpg)
![Western Blot: VPS35 Antibody (HL1017) [NBP3-13680] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS35 antibody [HL1017] (NBP3-13680) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/VPS35-Antibody-HL1017-Western-Blot-NBP3-13680-img0002.jpg)
![Western Blot: VPS35 Antibody (HL1017) [NBP3-13680] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS35 antibody [HL1017] (NBP3-13680) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/VPS35-Antibody-HL1017-Western-Blot-NBP3-13680-img0003.jpg)
![Western Blot: VPS35 Antibody (HL1017) [NBP3-13680] - Whole zebrafish extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS35 antibody [HL1017] (NBP3-13680) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-13680_rabbit-vps35-mab-hl1017-910202419593560.jpg)
![Immunohistochemistry-Paraffin: VPS35 Antibody (HL1017) [NBP3-13680] - VPS35 antibody [HL1017] detects VPS35 protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissue.VPS35 stained by VPS35 antibody [HL1017] (NBP3-13680) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-13680_rabbit-vps35-mab-hl1017-910202420131291.jpg)
![Immunohistochemistry-Paraffin: VPS35 Antibody (HL1017) [NBP3-13680] - VPS35 antibody [HL1017] detects VPS35 protein by immunohistochemical analysis.Sample: Paraffin-embedded rat testis.VPS35 stained by VPS35 antibody [HL1017] (NBP3-13680) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-13680_rabbit-vps35-mab-hl1017-91020242091446.jpg)
![Western Blot: VPS35 Antibody (HL1017) [NBP3-13680] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS35 antibody [HL1017] (NBP3-13680) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13680_rabbit-vps35-mab-hl1017-91020242011446.jpg)
![Western Blot: AKT2 Antibody (4H7) [NBP2-02231] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AKT2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AKT2.](http://images.novusbio.com/fullsize/AKT2-Antibody-4H7-Western-Blot-NBP2-02231-img0003.jpg "Western Blot: AKT2 Antibody (4H7) [NBP2-02231] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AKT2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AKT2.")
![Immunocytochemistry/Immunofluorescence: AKT2 Antibody (4H7) [NBP2-02231] Staining of COS7 cells transiently transfected by pCMV6-ENTRY AKT2.](http://images.novusbio.com/fullsize/AKT2-Antibody-4H7-Immunocytochemistry-Immunofluorescence-NBP2-02231-img0007.jpg)
![Immunohistochemistry-Paraffin: AKT2 Antibody (4H7) [NBP2-02231] - Staining of paraffin-embedded Human liver tissue using anti-AKT2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/AKT2-Antibody-4H7-Immunohistochemistry-Paraffin-NBP2-02231-img0006.jpg)
![Flow Cytometry: AKT2 Antibody (4H7) [NBP2-02231] - Analysis of Jurkat cells, using anti-AKT2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/AKT2-Antibody-4H7-Flow-Cytometry-NBP2-02231-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: AKT2 Antibody (4H7) [NBP2-02231] - Immunofluorescent staining of HepG2 cells using anti-AKT2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/AKT2-Antibody-4H7-Immunocytochemistry-Immunofluorescence-NBP2-02231-img0004.jpg)
![Immunohistochemistry-Paraffin: AKT2 Antibody (4H7) [NBP2-02231] - Staining of paraffin-embedded Human Kidney tissue using anti-AKT2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/AKT2-Antibody-4H7-Immunohistochemistry-Paraffin-NBP2-02231-img0005.jpg)
![Flow Cytometry: AKT2 Antibody (4H7) [NBP2-02231] - Analysis of Hela cells, using anti-AKT2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/AKT2-Antibody-4H7-Flow-Cytometry-NBP2-02231-img0001.jpg)
![Western Blot: MEK2 Antibody (8G6) [NBP1-47836] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MEK2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MEK2.](http://images.novusbio.com/fullsize/MEK2-Antibody-8G6-Western-Blot-NBP1-47836-img0028.jpg "Western Blot: MEK2 Antibody (8G6) [NBP1-47836] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MEK2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MEK2.")
![Immunohistochemistry-Paraffin: MEK2 Antibody (8G6) [NBP1-47836] - Staining of paraffin-embedded Human lung tissue using anti-MEK2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/MEK2-Antibody-8G6-Immunohistochemistry-Paraffin-NBP1-47836-img0026.jpg)
![Flow Cytometry: MEK2 Antibody (8G6) [NBP1-47836] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-MEK2 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/MEK2-Antibody-8G6-Flow-Cytometry-NBP1-47836-img0015.jpg)
![Immunohistochemistry-Paraffin: MEK2 Antibody (8G6) [NBP1-47836] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-MEK2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/MEK2-Antibody-8G6-Immunohistochemistry-Paraffin-NBP1-47836-img0016.jpg)
![Immunohistochemistry-Paraffin: MEK2 Antibody (8G6) [NBP1-47836] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-MEK2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/MEK2-Antibody-8G6-Immunohistochemistry-Paraffin-NBP1-47836-img0017.jpg)
![Immunohistochemistry-Paraffin: MEK2 Antibody (8G6) [NBP1-47836] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-MEK2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/MEK2-Antibody-8G6-Immunohistochemistry-Paraffin-NBP1-47836-img0018.jpg)
![Immunohistochemistry-Paraffin: MEK2 Antibody (8G6) [NBP1-47836] - Staining of paraffin-embedded Human endometrium tissue using anti-MEK2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/MEK2-Antibody-8G6-Immunohistochemistry-Paraffin-NBP1-47836-img0024.jpg)
![Immunohistochemistry-Paraffin: MEK2 Antibody (8G6) [NBP1-47836] - Staining of paraffin-embedded Human Kidney tissue using anti-MEK2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/MEK2-Antibody-8G6-Immunohistochemistry-Paraffin-NBP1-47836-img0025.jpg)
![Western Blot: PCNA Antibody (3D6) [NBP2-46342] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PCNA.](http://images.novusbio.com/fullsize/PCNA-Antibody-3D6-Western-Blot-NBP2-46342-img0022.jpg "Western Blot: PCNA Antibody (3D6) [NBP2-46342] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PCNA.")
![Immunohistochemistry: PCNA Antibody (3D6) [NBP2-46342] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PCNA-Antibody-3D6-Immunohistochemistry-NBP2-46342-img0021.jpg)
![Western Blot: PCNA Antibody (3D6) [NBP2-46342] - Analysis of extracts (35ug) from 9 different cell lines by using PCNA monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human). Dilution: 1:2000](http://images.novusbio.com/fullsize/PCNA-Antibody-3D6-Western-Blot-NBP2-46342-img0023.jpg)
![Immunohistochemistry: PCNA Antibody (3D6) [NBP2-46342] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PCNA-Antibody-3D6-Immunohistochemistry-NBP2-46342-img0001.jpg)
![Immunohistochemistry: PCNA Antibody (3D6) [NBP2-46342] - Analysis of Carcinoma of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PCNA-Antibody-3D6-Immunohistochemistry-NBP2-46342-img0002.jpg)
![Immunohistochemistry: PCNA Antibody (3D6) [NBP2-46342] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PCNA-Antibody-3D6-Immunohistochemistry-NBP2-46342-img0003.jpg)
![Immunohistochemistry: PCNA Antibody (3D6) [NBP2-46342] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PCNA-Antibody-3D6-Immunohistochemistry-NBP2-46342-img0004.jpg)
![Immunohistochemistry: PCNA Antibody (3D6) [NBP2-46342] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PCNA-Antibody-3D6-Immunohistochemistry-NBP2-46342-img0005.jpg)
![Immunohistochemistry: PCNA Antibody (3D6) [NBP2-46342] - Analysis of Carcinoma of Human kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PCNA-Antibody-3D6-Immunohistochemistry-NBP2-46342-img0006.jpg)
![Immunohistochemistry: PCNA Antibody (3D6) [NBP2-46342] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PCNA-Antibody-3D6-Immunohistochemistry-NBP2-46342-img0007.jpg)
![Immunohistochemistry: PCNA Antibody (3D6) [NBP2-46342] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PCNA-Antibody-3D6-Immunohistochemistry-NBP2-46342-img0008.jpg)
![Immunohistochemistry: PCNA Antibody (3D6) [NBP2-46342] - Analysis of Human Ovary tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PCNA-Antibody-3D6-Immunohistochemistry-NBP2-46342-img0009.jpg)
![Immunohistochemistry: PCNA Antibody (3D6) [NBP2-46342] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PCNA-Antibody-3D6-Immunohistochemistry-NBP2-46342-img0010.jpg)
![Immunohistochemistry: PCNA Antibody (3D6) [NBP2-46342] - Analysis of Human bladder tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PCNA-Antibody-3D6-Immunohistochemistry-NBP2-46342-img0011.jpg)
![Immunohistochemistry: PCNA Antibody (3D6) [NBP2-46342] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PCNA-Antibody-3D6-Immunohistochemistry-NBP2-46342-img0012.jpg)
![Immunohistochemistry: PCNA Antibody (3D6) [NBP2-46342] - Analysis of Carcinoma of Human bladder tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PCNA-Antibody-3D6-Immunohistochemistry-NBP2-46342-img0013.jpg)
![Immunohistochemistry: PCNA Antibody (3D6) [NBP2-46342] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PCNA-Antibody-3D6-Immunohistochemistry-NBP2-46342-img0014.jpg)
![Immunohistochemistry: PCNA Antibody (3D6) [NBP2-46342] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PCNA-Antibody-3D6-Immunohistochemistry-NBP2-46342-img0015.jpg)
![Immunohistochemistry: PCNA Antibody (3D6) [NBP2-46342] - Analysis of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PCNA-Antibody-3D6-Immunohistochemistry-NBP2-46342-img0016.jpg)
![Immunohistochemistry: PCNA Antibody (3D6) [NBP2-46342] - Analysis of Carcinoma of Human liver tissue.(Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PCNA-Antibody-3D6-Immunohistochemistry-NBP2-46342-img0017.jpg)
![Immunohistochemistry: PCNA Antibody (3D6) [NBP2-46342] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PCNA-Antibody-3D6-Immunohistochemistry-NBP2-46342-img0018.jpg)
![Immunohistochemistry: PCNA Antibody (3D6) [NBP2-46342] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PCNA-Antibody-3D6-Immunohistochemistry-NBP2-46342-img0019.jpg)
![Immunohistochemistry: PCNA Antibody (3D6) [NBP2-46342] - Analysis of Carcinoma of Human lung tissue.(Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PCNA-Antibody-3D6-Immunohistochemistry-NBP2-46342-img0020.jpg)
![Western Blot: c-Myc Antibody (Myc.A7) [NBP2-37822] - Analysis using the HRP conjugate of NBP2-37822. Detection of Myc Epitope Tag was performed by loading various amounts of E. coli lysate containing a multi-epitope tagged protein per well onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with an HRP-conjugated Myc Epitope Tag monoclonal antibody at a dilution of 1:1000 overnight at 4?C on a rocking platform and washed in TBS-0.1% Tween-20. Chemiluminescent detection was performed using SuperSignal West Pico.](http://images.novusbio.com/fullsize/c-Myc-Antibody-Myc-A7-Western-Blot-NBP2-37822-img0011.jpg "Western Blot: c-Myc Antibody (Myc.A7) [NBP2-37822] - Analysis using the HRP conjugate of NBP2-37822. Detection of Myc Epitope Tag was performed by loading various amounts of E. coli lysate containing a multi-epitope tagged protein per well onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with an HRP-conjugated Myc Epitope Tag monoclonal antibody at a dilution of 1:1000 overnight at 4?C on a rocking platform and washed in TBS-0.1% Tween-20. Chemiluminescent detection was performed using SuperSignal West Pico.")
![Immunocytochemistry/Immunofluorescence: c-Myc Antibody (Myc.A7) [NBP2-37822] - Analysis using the DyLight 680 conjugate of NBP2-37822. Staining of HeLa cells transfected with a construct containing a Myc Epitope Tag. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature.](http://images.novusbio.com/fullsize/c-Myc-Antibody-Myc-A7-Immunocytochemistry-Immunofluorescence-NBP2-37822-img0009.jpg)
![Flow Cytometry: c-Myc Antibody (Myc.A7) [NBP2-37822] - An intracellular stain was performed on Jurkat cells with c-Myc Antibody (Myc.A7) NBP2-37822AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/c-Myc-Antibody-Myc-A7-Flow-Cytometry-NBP2-37822-img0013.jpg)
![Western Blot: c-Myc Antibody (Myc.A7) [NBP2-37822] - Analysis of 1:1000 (1ug/mL) Ab dilution probed against HEK293 cells transfected with Myc-tagged protein vector; untransfected (1) and transfected (2)](http://images.novusbio.com/fullsize/c-Myc-Antibody-Myc-A7-Western-Blot-NBP2-37822-img0005.jpg)
![Western Blot: c-Myc Antibody (Myc.A7) [NBP2-37822] - Analysis using the DyLight 680 conjugate of NBP2-37822. Detection of Myc Epitope Tag was performed by loading various amounts of E. coli lysate containing a multi-epitope tagged protein per well onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a DyLight 680-conjugated Myc Epitope Tag monoclonal antibody at a dilution of 1:1000 for 1 hour at room temperature on a rocking platform and washed in TBS-0.1% Tween-20. Detection was performed using the LI-COR Odyssey.](http://images.novusbio.com/fullsize/c-Myc-Antibody-Myc-A7-Western-Blot-NBP2-37822-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: c-Myc Antibody (Myc.A7) [NBP2-37822] - Analysis using the DyLight 488 conjugate of NBP2-37822. Staining of HeLa cells transfected with a construct containing a Myc Epitope Tag. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature.](http://images.novusbio.com/fullsize/c-Myc-Antibody-Myc-A7-Immunocytochemistry-Immunofluorescence-NBP2-37822-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: c-Myc Antibody (Myc.A7) [NBP2-37822] - Analysis using the DyLight 550 conjugate of NBP2-37822. Staining of HeLa cells transfected with a construct containing a Myc Epitope Tag. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at](http://images.novusbio.com/fullsize/c-Myc-Antibody-Myc-A7-Immunocytochemistry-Immunofluorescence-NBP2-37822-img0008.jpg)
![Flow Cytometry: c-Myc Antibody (Myc.A7) [NBP2-37822] - An intracellular stain was performed on HepG2 cells with c-Myc Antibody (Myc.A7) NBP2-37822AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/c-Myc-Antibody-Myc-A7-Flow-Cytometry-NBP2-37822-img0012.jpg)
![Western Blot: VPS35 Antibody (8A3) [NBP2-78825] - Western Blot analysis of Human SH-SY5Y showing detection of VPS35 protein using Mouse Anti-VPS35 Monoclonal Antibody, Clone 8A3 (NBP2-78825). Lane 1: Molecular Weight Ladder. Lane 2: SH-SY5Y (10 ug). Load: 10 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody (NBP2-78825) at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT.](http://images.novusbio.com/fullsize/VPS35-Antibody-8A3-Western-Blot-NBP2-78825-img0012.jpg "Western Blot: VPS35 Antibody (8A3) [NBP2-78825] - Western Blot analysis of Human SH-SY5Y showing detection of VPS35 protein using Mouse Anti-VPS35 Monoclonal Antibody, Clone 8A3 (NBP2-78825). Lane 1: Molecular Weight Ladder. Lane 2: SH-SY5Y (10 ug). Load: 10 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody (NBP2-78825) at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT.")
![Immunohistochemistry: VPS35 Antibody (8A3) [NBP2-78825] - Immunohistochemistry analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 8A3 (NBP2-78825). Tissue: Kidney. Species: Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody (NBP2-78825) at 1:100 for Overnight at 4C, then 30 min at 37C. Secondary Antibody: Goat Anti-Mouse IgG (H+L): FITC for 45 min at 37C. Counterstain: DAPI for 3 min at RT. Magnification: 20X.](http://images.novusbio.com/fullsize/VPS35-Antibody-8A3-Immunohistochemistry-NBP2-78825-img0013.jpg)
![Western Blot: VPS35 Antibody (8A3) [NBP2-78825] - Western Blot analysis of Human, Mouse A549, MEF showing detection of VPS35 protein using Mouse Anti-VPS35 Monoclonal Antibody, Clone 8A3 (NBP2-78825). Lane 1: Molecular Weight Ladder. Lane 2: VPS35 KO A549 cells. Lane 3: mouse embryonic fibroblast cells.. Load: 8 ug each A549 and MEF. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody (NBP2-78825) at 1:5 (tissue culture supernatant). Secondary Antibody: Donkey anti-mouse IRDye 800CW at 1:25000 in TBS-T.](http://images.novusbio.com/fullsize/VPS35-Antibody-8A3-Western-Blot-NBP2-78825-img0011.jpg)
![Immunoprecipitation: VPS35 Antibody (8A3) [NBP2-78825] - Tissue: embryonic fibroblast. Species: Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody (NBP2-78825). Three amounts of NBP2-78825 (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4 degrees C and next incubated with 250ug of MEF lysate for 2 hours at 4 degrees C.](http://images.novusbio.com/fullsize/VPS35-Antibody-8A3-Immunoprecipitation-NBP2-78825-img0001.jpg)
![Immunoprecipitation: VPS35 Antibody (8A3) [NBP2-78825] - Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 8A3. Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. 500 uL cell culture supernatants were incubated with 10 uL of Protein A/G resin beads for 1 hour at 4C. clone 8A3 depletes VPS35 from the A549 cell extract.](http://images.novusbio.com/fullsize/VPS35-Antibody-8A3-Immunoprecipitation-NBP2-78825-img0008.jpg)
![Immunoprecipitation: VPS35 Antibody (8A3) [NBP2-78825] - Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 8A3. Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody.](http://images.novusbio.com/fullsize/VPS35-Antibody-8A3-Immunoprecipitation-NBP2-78825-img0009.jpg)
![Immunoprecipitation: VPS35 Antibody (8A3) [NBP2-78825] - Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 8A3. Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts of NBP2-78825 (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4C and next incubated with 250ug of A549 lysate for 2 hours at 4C.](http://images.novusbio.com/fullsize/VPS35-Antibody-8A3-Immunoprecipitation-NBP2-78825-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: LAMP-1/CD107a Antibody - BSA Free [NB600-956] - Human HeLa cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.4% Saponin. Fixed cells were stained with 5 ug/mL Anti-LAMP1 and developed with Goat Anti-Rabbit IgG, Cy3 conjugate.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NB600-956-img0006.jpg "Immunocytochemistry/Immunofluorescence: LAMP-1/CD107a Antibody - BSA Free [NB600-956] - Human HeLa cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.4% Saponin. Fixed cells were stained with 5 ug/mL Anti-LAMP1 and developed with Goat Anti-Rabbit IgG, Cy3 conjugate.")
![Immunocytochemistry/Immunofluorescence: LAMP-1/CD107a Antibody - BSA Free [NB600-956] - Rat NRK cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.4% Saponin. Fixed cells were stained with 10 ug/mL Anti-LAMP1 and developed with Goat Anti-Rabbit IgG, FITC conjugate.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NB600-956-img0005.jpg)
![ELISA: TNF-alpha ELISA Kit [Biotin] [NBP1-92681] - Standard curve](http://images.novusbio.com/fullsize/TNF-alpha-ELISA-Kit-[Biotin]-ELISA-NBP1-92681-img0001.jpg "ELISA: TNF-alpha ELISA Kit [Biotin] [NBP1-92681] - Standard curve")
![Western Blot: Huntingtin Antibody (7B9C8) [NBP3-15642] - Western blot analysis of extracts of various cell lines, using Huntingtin antibody (NBP3-15642) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Huntingtin-Antibody-7B9C8-Western-Blot-NBP3-15642-img0005.jpg "Western Blot: Huntingtin Antibody (7B9C8) [NBP3-15642] - Western blot analysis of extracts of various cell lines, using Huntingtin antibody (NBP3-15642) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunohistochemistry-Paraffin: Huntingtin Antibody (7B9C8) [NBP3-15642] - Immunohistochemistry of paraffin-embedded mouse spinal cord using Huntingtin Rabbit mAb (NBP3-15642) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Huntingtin-Antibody-7B9C8-Immunohistochemistry-Paraffin-NBP3-15642-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: Huntingtin Antibody (7B9C8) [Huntingtin] - Immunofluorescence analysis of Mouse brain tissue using Huntingtin Rabbit mAb at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. Microwave antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-15642_rabbit-huntingtin-mab-7b9c8-52202517344224.jpg)
![Western Blot: Huntingtin Antibody (7B9C8) [Huntingtin] - Western blot analysis of various lysates using Huntingtin Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-15642_rabbit-huntingtin-mab-7b9c8-52202517333895.jpg)
![Immunohistochemistry: Huntingtin Antibody (7B9C8) [Huntingtin] - Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using Huntingtin Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15642_rabbit-huntingtin-mab-7b9c8-52202517304947.jpg)
![Immunohistochemistry: Huntingtin Antibody (7B9C8) [Huntingtin] - Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using Huntingtin Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15642_rabbit-huntingtin-mab-7b9c8-52202517315522.jpg)
![Immunocytochemistry/ Immunofluorescence: Huntingtin Antibody (7B9C8) [Huntingtin] - Immunofluorescence analysis of Rat brain tissue using Huntingtin Rabbit mAb at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. Microwave antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-15642_rabbit-huntingtin-mab-7b9c8-52202517361214.jpg)
![Immunohistochemistry: Huntingtin Antibody (7B9C8) [Huntingtin] - Immunohistochemistry analysis of paraffin-embedded Human brain tissue using Huntingtin Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15642_rabbit-huntingtin-mab-7b9c8-52202517333868.jpg)
![Western Blot: Huntingtin Antibody (7B9C8) [Huntingtin] - Western blot analysis of various lysates using Huntingtin Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 3min.](http://images.novusbio.com/fullsize/nbp3-15642_rabbit-huntingtin-mab-7b9c8-5220251734569.jpg)