| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44492 | |||
| BSA Free | NBP2-59779-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-43822 | |||
| BSA Free | NBP2-46625-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-54653-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44493 | |||
| BSA Free | NBP2-59025-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-43819 | |||
| BSA Free | NBP2-46616-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-43821 | |||
| BSA Free | NBP2-46619-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-43861 | |||
| BSA Free | NBP2-61619-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Low Endotoxin, Azide and BSA Free | NBP2-75925SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-24837SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-27224 | |||
| BSA Free | NBP2-27223SS | |||
| Azide and BSA Free | NBP2-27224SS | |||
![Western Blot: GAPDH Antibody (CL3266) [NBP2-59779] - GAPDH Antibody (2597) [NBP2-59779] - Theoretical molecular weight: 36 kDa. Analysis of extracts from U-251 cells, transfected with: control siRNA, target specific siRNA probe #1, target specific siRNA probe #2, using Anti-GAPDH monoclonal antibody. Downregulation of antibody signal confirms target specificity. Remaining % intensity, relative control lane, is indicated. Anti-PPIB monoclonal antibody was used as loading control.](http://images.novusbio.com/fullsize/GAPDH-Antibody-CL3266-Western-Blot-NBP2-59779-img0009.jpg "<b>Genetic Strategies Validation. </b>Western Blot: GAPDH Antibody (CL3266) [NBP2-59779] - GAPDH Antibody (2597) [NBP2-59779] - Theoretical molecular weight: 36 kDa. Analysis of extracts from U-251 cells, transfected with: control siRNA, target specific siRNA probe #1, target specific siRNA probe #2, using Anti-GAPDH monoclonal antibody. Downregulation of antibody signal confirms target specificity. Remaining % intensity, relative control lane, is indicated. Anti-PPIB monoclonal antibody was used as loading control.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: GAPDH Antibody (CL3266) [NBP2-59779] - Staining of A549 cells using the Anti-GAPDH monoclonal antibody, showing specific staining in the nucleoplasm and cytosol in green. Microtubule and nuclear probes are visualized in red and blue, respectively (where available).](http://images.novusbio.com/fullsize/GAPDH-Antibody-CL3266-Immunocytochemistry-Immunofluorescence-NBP2-59779-img0013.jpg)
![Western Blot: GAPDH Antibody (CL3266) [NBP2-59779] - GAPDH Antibody (2597) [NBP2-59779] - Analysis in human cell line HeLa, human cell line HEK 293, human cell line A-431 and human cell line HepG2. Theoretical molecular weight: 36 kDa.](http://images.novusbio.com/fullsize/GAPDH-Antibody-CL3266-Western-Blot-NBP2-59779-img0008.jpg)
![Immunohistochemistry: GAPDH Antibody (CL3266) [NBP2-59779] - Human kidney shows cytoplasmic and nuclear immunoreactivity in renal tubules and glomerular cells.](http://images.novusbio.com/fullsize/GAPDH-Antibody-CL3266-Immunohistochemistry-NBP2-59779-img0002.jpg)
![Immunohistochemistry: GAPDH Antibody (CL3266) [NBP2-59779] - Human fallopian tube shows cytoplasmic and nuclear positivity in the epithelial and connective tissue cells.](http://images.novusbio.com/fullsize/GAPDH-Antibody-CL3266-Immunohistochemistry-NBP2-59779-img0003.jpg)
![Immunohistochemistry: GAPDH Antibody (CL3266) [NBP2-59779] - Human liver shows cytoplasmic and nuclear immunoreactivity in hepatocytes.](http://images.novusbio.com/fullsize/GAPDH-Antibody-CL3266-Immunohistochemistry-NBP2-59779-img0004.jpg)
![Immunohistochemistry: GAPDH Antibody (CL3266) [NBP2-59779] - Human pancreas shows nuclear and cytoplasmic immunoreactivity in both endocrine and exocrine cells.](http://images.novusbio.com/fullsize/GAPDH-Antibody-CL3266-Immunohistochemistry-NBP2-59779-img0005.jpg)
![Western Blot: NF-M Antibody (2705) [NBP2-46625] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Mouse Cerebral Cortex tissue](http://images.novusbio.com/fullsize/NF-M-Antibody-2705-Western-Blot-NBP2-46625-img0009.jpg "Western Blot: NF-M Antibody (2705) [NBP2-46625] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Mouse Cerebral Cortex tissue")
![Immunohistochemistry: NF-M Antibody (2705) [NBP2-46625] - Staining of human liver shows absence of immunoreactivity (negative control).](http://images.novusbio.com/fullsize/NF-M-Antibody-2705-Immunohistochemistry-NBP2-46625-img0007.jpg)
![Western Blot: NF-M Antibody (2705) [NBP2-46625] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Human Cerebral Cortex tissue](http://images.novusbio.com/fullsize/NF-M-Antibody-2705-Western-Blot-NBP2-46625-img0008.jpg)
![Immunohistochemistry: NF-M Antibody (2705) [NBP2-46625] - Staining of rat brainstem shows strong immunoreactivity in mesencephalic trigeminal nucleus neurons.](http://images.novusbio.com/fullsize/NF-M-Antibody-2705-Immunohistochemistry-NBP2-46625-img0001.jpg)
![Immunohistochemistry: NF-M Antibody (2705) [NBP2-46625] - Staining of mouse cerebral cortex shows strong immunoreactivity in neurofilaments.](http://images.novusbio.com/fullsize/NF-M-Antibody-2705-Immunohistochemistry-NBP2-46625-img0002.jpg)
![Immunohistochemistry: NF-M Antibody (2705) [NBP2-46625] - Staining of human cerebellum shows strong immunoreactivity in Purkinje cells and neuronal processes.](http://images.novusbio.com/fullsize/NF-M-Antibody-2705-Immunohistochemistry-NBP2-46625-img0003.jpg)
![Immunohistochemistry: NF-M Antibody (2705) [NBP2-46625] - Staining of rat cerebellum shows strong immunoreactivity in neuronal processes.](http://images.novusbio.com/fullsize/NF-M-Antibody-2705-Immunohistochemistry-NBP2-46625-img0004.jpg)
![Immunohistochemistry: NF-M Antibody (2705) [NBP2-46625] - Staining of mouse trigeminal ganglion shows strong positivity in primary sensory neurons.](http://images.novusbio.com/fullsize/NF-M-Antibody-2705-Immunohistochemistry-NBP2-46625-img0005.jpg)
![Immunohistochemistry: NF-M Antibody (2705) [NBP2-46625] - Staining of human cerebral cortex shows strong positivity in neural fibers.](http://images.novusbio.com/fullsize/NF-M-Antibody-2705-Immunohistochemistry-NBP2-46625-img0006.jpg)
![Western Blot: APE Antibody [NBP2-54653] - Lane 1: Marker [kDa] 206, 113, 82, 49, 32, 26, 18 Lane 2: Human cell line RT-4 Lane 3: Human cell line U-251MG sp Lane 4: Human cell line A-431 Lane 5: Human liver tissue Lane 6: Human tonsil tissue](http://images.novusbio.com/fullsize/APE-Antibody-Western-Blot-NBP2-54653-img0005.jpg "Western Blot: APE Antibody [NBP2-54653] - Lane 1: Marker [kDa] 206, 113, 82, 49, 32, 26, 18 Lane 2: Human cell line RT-4 Lane 3: Human cell line U-251MG sp Lane 4: Human cell line A-431 Lane 5: Human liver tissue Lane 6: Human tonsil tissue")
![Immunocytochemistry/Immunofluorescence: APE Antibody [NBP2-54653] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus.](http://images.novusbio.com/fullsize/APE-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-54653-img0007.jpg)
![Immunohistochemistry-Paraffin: APE Antibody [NBP2-54653] - Staining of human skeletal muscle shows low expression as expected.](http://images.novusbio.com/fullsize/APE-Antibody-Immunohistochemistry-Paraffin-NBP2-54653-img0009.jpg)
![Western Blot: APE Antibody [NBP2-54653] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells) Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)](http://images.novusbio.com/fullsize/APE-Antibody-Western-Blot-NBP2-54653-img0002.jpg)
![Immunohistochemistry-Paraffin: APE Antibody [NBP2-54653] - Staining of human small intestine shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/APE-Antibody-Immunohistochemistry-Paraffin-NBP2-54653-img0001.jpg)
![Immunohistochemistry-Paraffin: APE Antibody [NBP2-54653] - Staining of human endometrium shows high expression.](http://images.novusbio.com/fullsize/APE-Antibody-Immunohistochemistry-Paraffin-NBP2-54653-img0008.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: APE Antibody [NBP2-54653] - Staining in human endometrium and skeletal muscle tissues using anti-APEX1 antibody. Corresponding APEX1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/APE-Antibody-Immunohistochemistry-Paraffin-NBP2-54653-img0010.jpg)
![Western Blot: GAPDH Antibody (CL3265) [NBP2-59025] - Theoretical molecular weight: 36 kDa. Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-GAPDH antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.](http://images.novusbio.com/fullsize/GAPDH-Antibody-CL3265-Western-Blot-NBP2-59025-img0008.jpg "<b>Genetic Strategies Validation. </b>Western Blot: GAPDH Antibody (CL3265) [NBP2-59025] - Theoretical molecular weight: 36 kDa. Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-GAPDH antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: GAPDH Antibody (CL3265) [NBP2-59025] - Staining of human skeletal muscle shows moderate cytoplasmic and strong nuclear immunoreactivity in muscle fibers.](http://images.novusbio.com/fullsize/GAPDH-Antibody-CL3265-Immunohistochemistry-Paraffin-NBP2-59025-img0005.jpg)
![Western Blot: GAPDH Antibody (CL3265) [NBP2-59025] - Analysis in human cell line HeLa, human cell line HEK 293, human cell line A-431, human cell line HepG2, mouse cell line NIH-3T3 and rat cell line NBT-II. Theoretical molecular weight: 36 kDa.](http://images.novusbio.com/fullsize/GAPDH-Antibody-CL3265-Western-Blot-NBP2-59025-img0006.jpg)
![Immunohistochemistry-Paraffin: GAPDH Antibody (CL3265) [NBP2-59025] - Staining of human cerebral cortex shows immunoreactivity in neuropil as well as strong cytoplasmic positivity in a subset of cells.](http://images.novusbio.com/fullsize/GAPDH-Antibody-CL3265-Immunohistochemistry-Paraffin-NBP2-59025-img0001.jpg)
![Immunohistochemistry-Paraffin: GAPDH Antibody (CL3265) [NBP2-59025] - Staining of human kidney shows strong cytoplasmic and nuclear positivity in renal tubules and glomeruli.](http://images.novusbio.com/fullsize/GAPDH-Antibody-CL3265-Immunohistochemistry-Paraffin-NBP2-59025-img0002.jpg)
![Immunohistochemistry-Paraffin: GAPDH Antibody (CL3265) [NBP2-59025] - Staining of human rectum shows strong cytoplasmic and nuclear positivity in glandular and connective tissue cells.](http://images.novusbio.com/fullsize/GAPDH-Antibody-CL3265-Immunohistochemistry-Paraffin-NBP2-59025-img0003.jpg)
![Immunohistochemistry-Paraffin: GAPDH Antibody (CL3265) [NBP2-59025] - Staining of human liver shows strong cytoplasmic and nuclear immunoreactivity in hepatocytes.](http://images.novusbio.com/fullsize/GAPDH-Antibody-CL3265-Immunohistochemistry-Paraffin-NBP2-59025-img0004.jpg)
![Western Blot: NF-M Antibody (2678) [NBP2-46616] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Mouse Cerebral Cortex tissue](http://images.novusbio.com/fullsize/NF-M-Antibody-2678-Western-Blot-NBP2-46616-img0009.jpg "Western Blot: NF-M Antibody (2678) [NBP2-46616] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Mouse Cerebral Cortex tissue")
![Immunohistochemistry-Paraffin: NF-M Antibody (2678) [NBP2-46616] - Staining of rat brainstem shows strong immunoreactivity in neurons of mesencephalic trigeminal nucleus.](http://images.novusbio.com/fullsize/NF-M-Antibody-2678-Immunohistochemistry-Paraffin-NBP2-46616-img0011.jpg)
![Western Blot: NF-M Antibody (2678) [NBP2-46616] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Human Cerebral Cortex tissue](http://images.novusbio.com/fullsize/NF-M-Antibody-2678-Western-Blot-NBP2-46616-img0008.jpg)
![Immunohistochemistry: NF-M Antibody (2678) [NBP2-46616] - Staining of mouse cerebral cortex shows strong immunoreactivity in neurofilaments.](http://images.novusbio.com/fullsize/NF-M-Antibody-2678-Immunohistochemistry-NBP2-46616-img0002.jpg)
![Immunohistochemistry: NF-M Antibody (2678) [NBP2-46616] - Staining of human cerebral cortex shows strong positivity in neural fibers.](http://images.novusbio.com/fullsize/NF-M-Antibody-2678-Immunohistochemistry-NBP2-46616-img0003.jpg)
![Immunohistochemistry: NF-M Antibody (2678) [NBP2-46616] - Staining of rat cerebellum shows strong immunoreactivity in neuronal processes.](http://images.novusbio.com/fullsize/NF-M-Antibody-2678-Immunohistochemistry-NBP2-46616-img0004.jpg)
![Immunohistochemistry: NF-M Antibody (2678) [NBP2-46616] - Staining of mouse trigeminal ganglion shows strong immunoreactivity in ganglion cells.](http://images.novusbio.com/fullsize/NF-M-Antibody-2678-Immunohistochemistry-NBP2-46616-img0005.jpg)
![Immunohistochemistry: NF-M Antibody (2678) [NBP2-46616] - Staining of human cerebellum shows strong immunoreactivity in Purkinje cells and neural fibers.](http://images.novusbio.com/fullsize/NF-M-Antibody-2678-Immunohistochemistry-NBP2-46616-img0006.jpg)
![Immunohistochemistry: NF-M Antibody (2678) [NBP2-46616] - Staining of human tonsil shows absence of immunoreactivity (negative control).](http://images.novusbio.com/fullsize/NF-M-Antibody-2678-Immunohistochemistry-NBP2-46616-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: PIST Antibody [NBP1-88223] - Immunofluorescent staining of human cell line U-2 OS shows localization to the Golgi apparatus.](http://images.novusbio.com/fullsize/PIST-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88223-img0008.jpg "Immunocytochemistry/Immunofluorescence: PIST Antibody [NBP1-88223] - Immunofluorescent staining of human cell line U-2 OS shows localization to the Golgi apparatus.")
![Western Blot: NF-M Antibody (2697) [NBP2-46619] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Mouse Cerebral Cortex tissue](http://images.novusbio.com/fullsize/NF-M-Antibody-2697-Western-Blot-NBP2-46619-img0009.jpg "Western Blot: NF-M Antibody (2697) [NBP2-46619] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Mouse Cerebral Cortex tissue")
![Immunohistochemistry: NF-M Antibody (2697) [NBP2-46619] - Staining of human liver shows absence of immunoreactivity (negative control).](http://images.novusbio.com/fullsize/NF-M-Antibody-2697-Immunohistochemistry-NBP2-46619-img0007.jpg)
![Western Blot: NF-M Antibody (2697) [NBP2-46619] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Human Cerebral Cortex tissue](http://images.novusbio.com/fullsize/NF-M-Antibody-2697-Western-Blot-NBP2-46619-img0008.jpg)
![Immunohistochemistry: NF-M Antibody (2697) [NBP2-46619] - Staining of rat cerebral cortex shows strong immunoreactivity in neuronal processes.](http://images.novusbio.com/fullsize/NF-M-Antibody-2697-Immunohistochemistry-NBP2-46619-img0001.jpg)
![Immunohistochemistry: NF-M Antibody (2697) [NBP2-46619] - Staining of mouse cerebral cortex shows strong immunoreactivity in neuronal processes.](http://images.novusbio.com/fullsize/NF-M-Antibody-2697-Immunohistochemistry-NBP2-46619-img0002.jpg)
![Immunohistochemistry: NF-M Antibody (2697) [NBP2-46619] - Staining of human cerebral cortex shows strong positivity in neural fibers.](http://images.novusbio.com/fullsize/NF-M-Antibody-2697-Immunohistochemistry-NBP2-46619-img0003.jpg)
![Immunohistochemistry: NF-M Antibody (2697) [NBP2-46619] - Staining of rat striatum shows strong positivity in neural fibers.](http://images.novusbio.com/fullsize/NF-M-Antibody-2697-Immunohistochemistry-NBP2-46619-img0004.jpg)
![Immunohistochemistry: NF-M Antibody (2697) [NBP2-46619] - Staining of mouse trigeminal ganglion shows strong positivity in primary sensory neurons.](http://images.novusbio.com/fullsize/NF-M-Antibody-2697-Immunohistochemistry-NBP2-46619-img0005.jpg)
![Immunohistochemistry: NF-M Antibody (2697) [NBP2-46619] - Staining of human cerebellum shows strong immunoreactivity in Purkinje cells and neuronal processes.](http://images.novusbio.com/fullsize/NF-M-Antibody-2697-Immunohistochemistry-NBP2-46619-img0006.jpg)
![Western Blot: LAMP-1/CD107a Antibody (CL3482) [NBP2-61619] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human cell line RT-4](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-CL3482-Immunohistochemistry-Paraffin-NBP2-61619-img0007.jpg "Western Blot: LAMP-1/CD107a Antibody (CL3482) [NBP2-61619] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human cell line RT-4")
![Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (CL3482) [NBP2-61619] - Staining of human skeletal muscle shows only very low cytoplasmic immunoreactivity.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-CL3482-Immunohistochemistry-Paraffin-NBP2-61619-img0006.jpg)
![Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (CL3482) [NBP2-61619] - Staining of human kidney shows strong granular cytoplasmic immunoreactivity in renal glomerulus and tubules cells.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-CL3482-Immunohistochemistry-NBP2-61619-img0001.jpg)
![Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (CL3482) [NBP2-61619] - Staining of human liver shows strong granular cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-CL3482-Immunohistochemistry-NBP2-61619-img0002.jpg)
![Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (CL3482) [NBP2-61619] - Staining of human fallopian tube shows granular cytoplasmic immunoreactivity in epithelial cells.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-CL3482-Immunohistochemistry-NBP2-61619-img0003.jpg)
![Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (CL3482) [NBP2-61619] - Staining of human cerebral cortex shows strong granular cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-CL3482-Immunohistochemistry-NBP2-61619-img0004.jpg)
![Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (CL3482) [NBP2-61619] - Staining of human small intestine shows granular cytoplasmic immunoreactivity in glandular cells.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-CL3482-Immunohistochemistry-Paraffin-NBP2-61619-img0005.jpg)
![Western Blot: TNF-alpha Antibody (2342C) - Low Endotoxin and Azide Free [NBP2-75925] - Western blot shows conditioned media from CHO Chinese hamster ovary cell line either mock transfected or transfected with human TNF- alpha. PVDF membrane was probed with 2 ug/mL of Rabbit Anti-Human TNF-alpha Monoclonal Antibody (NBP2-75925) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for TNF-alpha at approximately 18 and 21 kDa (as indicated). This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/TNF-alpha-Antibody-2342C-Low-Endotoxin-and-Azide-Free-Western-Blot-NBP2-75925-img0002.jpg "Western Blot: TNF-alpha Antibody (2342C) - Low Endotoxin and Azide Free [NBP2-75925] - Western blot shows conditioned media from CHO Chinese hamster ovary cell line either mock transfected or transfected with human TNF- alpha. PVDF membrane was probed with 2 ug/mL of Rabbit Anti-Human TNF-alpha Monoclonal Antibody (NBP2-75925) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for TNF-alpha at approximately 18 and 21 kDa (as indicated). This experiment was conducted under reducing conditions.")
![Immunohistochemistry-Paraffin: TNF-alpha Antibody (2342C) - Low Endotoxin and Azide Free [NBP2-75925] - Analysis of a FFPE tissue section of the human intesting using 1:200 dilution of TNF-alpha antibody (NBP2-75925). The signal was developed using HRP-DAB method which followed counterstaining of the cells with hematoxylin. The antibody generated a moderate to strong cytoplasmic staining in crypt cells.](http://images.novusbio.com/fullsize/TNF-alpha-Antibody-2342C-Low-Endotoxin-and-Azide-Free-Immunohistochemistry-Paraffin-NBP2-75925-img0003.jpg)
![Neutralization: TNF-alpha Antibody (2342C) [NBP2-75925] - Recombinant Human TNF-alpha (Catalog # 210-TA) induces cytotoxicity in the the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Cytotoxicity elicited by Recombinant Human TNF-alpha (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human TNF-alpha Monoclonal Antibody (Catalog # NBP2-75925). The ND50 is typically 1-10 ng/mL in the presence of the metabolic inhibitor actinomycin D.](http://images.novusbio.com/fullsize/TNF-alpha-Antibody-2342C-Neutralization-NBP2-75925-img0001.jpg)
![Western Blot: MEK1 [p Thr292] Antibody [NB500-145] - Recombinant wild type and mutant MEK 1 showing immunolabeling of the ~45k MEK-1 protein phosphorylated at Thr292. Lanes 1 and 2 are WT MEK 1 and Lanes 3 and 4 are mutant MEK 1 (T292A). MAP kinase was coexpressed in the samples run in Lanes 2 and 4.](http://images.novusbio.com/fullsize/MEK1-[p-Thr292]-Antibody-Western-Blot-NB500-145-img0001.jpg "Western Blot: MEK1 [p Thr292] Antibody [NB500-145] - Recombinant wild type and mutant MEK 1 showing immunolabeling of the ~45k MEK-1 protein phosphorylated at Thr292. Lanes 1 and 2 are WT MEK 1 and Lanes 3 and 4 are mutant MEK 1 (T292A). MAP kinase was coexpressed in the samples run in Lanes 2 and 4.")
![Western Blot: RPTOR Antibody [NBP2-24837] - Analysis of Raptor in A) human 293 and B) mouse NIH 3T3 lysate using Raptor antibody at 1:2000.](http://images.novusbio.com/fullsize/RPTOR-Antibody-Western-Blot-NBP2-24837-img0005.jpg "Western Blot: RPTOR Antibody [NBP2-24837] - Analysis of Raptor in A) human 293 and B) mouse NIH 3T3 lysate using Raptor antibody at 1:2000.")
![Immunocytochemistry/Immunofluorescence: RPTOR Antibody [NBP2-24837] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-RPTOR at a 1:100 overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/RPTOR-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-24837-img0006.jpg)
![Immunohistochemistry-Paraffin: RPTOR Antibody [NBP2-24837] - Analysis of human small intestine using Raptor antibody at 1:100.](http://images.novusbio.com/fullsize/RPTOR-Antibody-Immunohistochemistry-Paraffin-NBP2-24837-img0004.jpg)
![Western Blot: TNF-alpha Antibody (6N1E7) [NBP2-27223] - Analysis using Azide Free version of NBP2-27223. TNF alpha recombinant protein using TNF alpha antibody at 3 ug/mL. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/TNF-alpha-Antibody-6N1E7-Western-Blot-NBP2-27223-img0004.jpg "Western Blot: TNF-alpha Antibody (6N1E7) [NBP2-27223] - Analysis using Azide Free version of NBP2-27223. TNF alpha recombinant protein using TNF alpha antibody at 3 ug/mL. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![<b>Biological Strategies Validation. </b>Flow Cytometry: TNF-alpha Antibody (6N1E7) [NBP2-27223] - Human PBMC were stimulated with PMA (10 ng/mL) and Ionomycin (500 ng/mL) for 5h in presence of Brefeldin A. At the end of stimulation, cells were surface stained before testing by intracellular staining. Fixed and permeabilized cells were incubated with 10 uL (0.5 ug) of TNF- alpha antibody for 30 minutes on ice. Lymphocyte population was gated for analysis to show TNF alpha positive T cells.](http://images.novusbio.com/fullsize/TNF-alpha-Antibody-6N1E7-Flow-Cytometry-NBP2-27223-img0002.jpg)
![Inhibition Activity: TNF-alpha Antibody (6N1E7) [NBP2-27223] - Validation of inhibitory effects of TNF alpha antibody on TNF alpha activity in the cell line. NF-kB/SEAPorter cells were plated in 96-well plate at 5 x 10^4 cells per well for 16 h. Cells were pre-incubated with TNF alpha antibody at 10 and 20 ug/ml for 1 hr, then treated with 10 ng/ml TNF alpha or 10 ng/ml PMA. After 24h, the secreted alkaline phosphatase (SEAP) was analyzed using the SEAPorterAssay Kit . Image using the Azide Free format of this antibody.](http://images.novusbio.com/fullsize/TNF-alpha-Antibody-6N1E7-Inhibition-Activity-NBP2-27223-img0006.jpg)