| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor Plus 488 | NBP3-50140AFP488 | |||
| Alexa Fluor Plus 555 | NBP3-50140AFP555 | |||
| Alexa Fluor Plus 594 | NBP3-50140AFP594 | |||
| Alexa Fluor Plus 647 | NBP3-50140AFP647 | |||
| Alexa Fluor Plus 680 | NBP3-50140AFP680 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-74609 | |||
![Western Blot: RFP Antibody [NBP1-97371] - Antibody was added at 1.0ug/mL at RT for 2 hours. IRDye800(R) anti-Chicken was added at 1:20,000 at RT for 45 minutes.](http://images.novusbio.com/fullsize/RFP-Antibody-Western-Blot-NBP1-97371-img0001.jpg "Western Blot: RFP Antibody [NBP1-97371] - Antibody was added at 1.0ug/mL at RT for 2 hours. IRDye800(R) anti-Chicken was added at 1:20,000 at RT for 45 minutes.")
![Immunocytochemistry/Immunofluorescence: RFP Antibody [NBP1-97371]](http://images.novusbio.com/fullsize/RFP-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-97371-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: RFP Antibody [NBP1-97371]](http://images.novusbio.com/fullsize/RFP-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-97371-img0002.jpg)
![Western Blot: RFP Antibody [NBP1-69962] - Western blot of RFP recombinant protein detected with RFP antibody. Lane 1 shows no reaction against a GFP recombinant protein present in 10 ug of HeLa cell extract. Lane 2 shows a single band detected in 10 ug of a HeLa lysate containing RFP recombinant protein as a 27 kDa band. A 4-12% Bis-Tris gradient gel (Invitrogen) was used for SDS-PAGE. The membrane was blocked and then probed with Anti-RFP diluted 1:2,500 for 1 h at RT followed by washes and reaction with a 1:5,000 dilution of IRDye(TM)800 conjugated Goat-a-Rabbit IgG [H&L] MX. IRDye(TM)800 fluorescence image was captured using the Odyssey(R) Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.](http://images.novusbio.com/fullsize/RFP-Antibody-Western-Blot-NBP1-69962-img0025.jpg "Western Blot: RFP Antibody [NBP1-69962] - Western blot of RFP recombinant protein detected with RFP antibody. Lane 1 shows no reaction against a GFP recombinant protein present in 10 ug of HeLa cell extract. Lane 2 shows a single band detected in 10 ug of a HeLa lysate containing RFP recombinant protein as a 27 kDa band. A 4-12% Bis-Tris gradient gel (Invitrogen) was used for SDS-PAGE. The membrane was blocked and then probed with Anti-RFP diluted 1:2,500 for 1 h at RT followed by washes and reaction with a 1:5,000 dilution of IRDye(TM)800 conjugated Goat-a-Rabbit IgG [H&L] MX. IRDye(TM)800 fluorescence image was captured using the Odyssey(R) Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.")
![Immunocytochemistry/Immunofluorescence: RFP Antibody [NBP1-69962] - Immunofluorescence Microscopy of RFP antibody. Tissue: (10X) Mouse E14.5 embryo heart tissue.Fixation: 4% PFA. Antigen retrieval: Heat.Primary antibody: Anti-RFP antibody at 1:50 for 1 h at RT.Secondary antibody: Fluorescein rabbit secondary antibody at 1:250 for 1 hr at RT.Staining: cardiac cells are RFP positive in red in tomato transgenic mice.](http://images.novusbio.com/fullsize/RFP-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-69962-img0019.jpg)
![Immunohistochemistry: RFP Antibody [NBP1-69962] - Immunohistochemistry of RFP antibody.Tissue: Mouse gut tissue in tomato transgenic mice.Fixation: formalin fixed paraffin embedded. Antigen retrieval: heat 5 min at high temp in 1X rodent decloaker. Primary antibody: RFP antibody at 1:200 for 1 h 30 min at RT.Secondary Antibody: HRP anti-rabbit.](http://images.novusbio.com/fullsize/RFP-Antibody-Immunohistochemistry-NBP1-69962-img0026.jpg)
![Western Blot: RFP Antibody [NBP1-69962] - RFP recombinant protein detected with polyclonal anti-RFP antibody. Lane 1 shows no reaction against a GFP recombinant protein present in 10 ug of HeLa cell extract. Lane 2 shows a single band detected in 10 ug of a HeLa lysate containing RFP recombinant protein as a 27 kDa band. A 4-12% Bis-Tris gradient gel was used for SDS-PAGE. The membrane was blocked and then probed with Anti-RFP diluted 1:2,500 for 1 h at RT followed by washes and reaction with a 1:5,000 dilution of IRDye 800 conjugated Goat-a-Rabbit IgG [H&L] MX. IRDye 800 fluorescence image was captured using the Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.](http://images.novusbio.com/fullsize/RFP-Antibody-Western-Blot-NBP1-69962-img0008.jpg)
![Western Blot: RFP Antibody [NBP1-69962] - Western Blot of RFP antibody. Lane 1: RFP. Lane 2: Human IgG. Lane 3: Goat IgG. Lane 4: Mouse IgG. Load: 50ng per lane.Primary antibody: RFP antibody at 1:1,000 overnight at 4C.Secondary antibody: Peroxidase conjugated rabbit secondary antibody at 1:40,000 for 30 min at RT.Block Blocking Buffer for 30 min at RT.Predicted/Observed size: 27 kDa, 30 kDa.](http://images.novusbio.com/fullsize/RFP-Antibody-Western-Blot-NBP1-69962-img0021.jpg)
![Western Blot: RFP Antibody [NBP1-69962] - Western Blot of RFP antibody. Lane 1: Opal Prestained Marker.Lane 2: 50ng of RFP. Primary Antibody: Anti-RFP at 1ug/mL overnight at 2-8C. Secondary Antibody: Goat anti-Rabbit HRP at 1:70,000 for 30mins at RT. Block: BlockOut Universal blocking buffer. Expect ~27kDa.](http://images.novusbio.com/fullsize/RFP-Antibody-Western-Blot-NBP1-69962-img0022.jpg)
![Western Blot: RFP Antibody [NBP1-69962] - Western Blot of RFP antibody. Marker: Opal Pre-stained ladder. Lane 1: HEK293 lysate. Lane 2: HeLa Lysate. Lane 3: CHO/K1 Lysate. Lane 4: MDA-MB-231. Lane 5: A431 Lysate. Lane 6: Jurkat Lysate. Lane 7: NIH/3T3 Lysate. Lane 8: E-coli HCP Control. Lane 9: FLAG Positive Control Lysate. Lane 10: Red Fluorescent Protein. Lane 11: Green Fluorescent Protein. Lane 12: Glutathione-S-Transferase Protein. Lane 13: Maltose Binding Protein. Load: 10 ug of lysate or 50ng of purified protein per lane. Primary antibody: RFP antibody at 1ug/mL overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:30,000 for 60 min at RT. Blocking Buffer: 1% Casein-TTBS for 30 min at RT. Predicted/Observed size: 30 kDa for RFP.](http://images.novusbio.com/fullsize/RFP-Antibody-Western-Blot-NBP1-69962-img0023.jpg)
![Western Blot: RFP Antibody [NBP1-69962] - Western Blot of RFP antibody. Lane 1: Opal Prestained Molecular Weight Marker. Lane 2: RFP/HEK293T WCL [0.05/10ug] [+]. Lane 3: HEK293T Whole Cell Lysate [-]. Lane 4: RFP/NIH/3T3 Whole Cell Lysate [0.05/10ug] [+]. Lane 5: NIH/3T3 Whole Cell Lysate [-]. Lane 6: RFP/PC-12 Whole Cell Lysate [0.05/10ug] [+]. Lane 7: PC-12 Whole Cell Lysate [-]. Primary Antibody: Anti-RFP at 1:1000 overnight at 2-8C. Secondary Antibody: Goat Anti-Rabbit IgG Peroxidase at 1:70,000 at RT for 30 mins. Predicted/Observed MW: ~27kDa. Exposure: 2 sec.](http://images.novusbio.com/fullsize/RFP-Antibody-Western-Blot-NBP1-69962-img0024.jpg)
![Immunocytochemistry/Immunofluorescence: RFP Antibody [NBP1-69962] - Rabbit Anti-RFP antibody. Tissue: HopERCre/+; R26Tom/+ mice. Fixation: 0.5% PFA. Antigen retrieval: Tamoxifen. Primary antibody: RFP antibody at 10 ug/mL for 1 h at RT. Secondary antibody: Fluorescein rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: RFP is nuclear and occasionally cytoplasmic. Staining: Hop-derived cells in the hair follicle, labeled in red.](http://images.novusbio.com/fullsize/RFP-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-69962-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: RFP Antibody [NBP1-69962] - Rabbit Anti-RFP antibody. Tissue: (10X) Mouse lung tissue. Fixation: 4% PFA. Antigen retrieval: Heat. Primary antibody: Anti-RFP antibody at 1:50 for 1 h at RT. Secondary antibody: Fluorescein rabbit secondary antibody at 1:250 for 1 hr at RT. Staining: SPC+ cells are RFP positive in red.](http://images.novusbio.com/fullsize/RFP-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-69962-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: RFP Antibody [NBP1-69962] - Rabbit Anti-RFP antibody. Tissue: (10X) Mouse E14.5 embryo heart tissue. Fixation: 4% PFA. Antigen retrieval: Heat. Primary antibody: Anti-RFP antibody at 1:50 for 1 h at RT. Secondary antibody: Fluorescein rabbit secondary antibody at 1:250 for 1 hr at RT. Staining: cardiac cells are RFP positive in red in tomato transgenic mice.](http://images.novusbio.com/fullsize/RFP-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-69962-img0003.jpg)
![CyTOF-ready: RFP Antibody [NBP1-69962] - Analysis of RFP antibody on human tissue. RFP-expressing cells (red), nuclei (blue). Scale, 50 um. Image from verified customer review](http://images.novusbio.com/fullsize/RFP-Antibody-CyTOF-ready-NBP1-69962-img0016.jpg)
![ELISA: RFP Antibody [NBP1-69962] - ELISA Results of RFP antibody tested against purified RFP protein. Each well was coated in duplicate with 1.0 ug of RFP [red], Human IgG [green], Mouse IgG [blue], Rat IgG [purple]. The starting dilution of antibody was 5ug/mL and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 1% Fish Gel in PBS Blocking Buffer, Goat Anti-Rabbit IgG HRP conjugated and TMB substrate. Results show RFP titer >75,000, Human, Mouse, Rat IgG titers <100.](http://images.novusbio.com/fullsize/RFP-Antibody-ELISA-NBP1-69962-img0027.jpg)
![Immunoprecipitation: RFP Antibody (1610) [NBP2-43713] - Analysis of 400 ug cell lysate/extract of RFP-fused gene transfected 293T cells A. 30 ug lysate/extract of transfected 293T cell B. Control with 2 ug of preimmune mouse IgG C. Immunoprecipitation of RFP-fused protein by 2 ug of RFP tag antibody 12% SDS-PAGE The immunoprecipitated RFP-fused protein was detected by RFP tag antibody diluted at 1:1000. EasyBlot anti-nouse IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/RFP-Antibody-1610-Immunoprecipitation-NBP2-43713-img0002.jpg "Immunoprecipitation: RFP Antibody (1610) [NBP2-43713] - Analysis of 400 ug cell lysate/extract of RFP-fused gene transfected 293T cells A. 30 ug lysate/extract of transfected 293T cell B. Control with 2 ug of preimmune mouse IgG C. Immunoprecipitation of RFP-fused protein by 2 ug of RFP tag antibody 12% SDS-PAGE The immunoprecipitated RFP-fused protein was detected by RFP tag antibody diluted at 1:1000. EasyBlot anti-nouse IgG was used as a secondary reagent.")
![Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with RFP antibody [GT1610] (NBP2-43713) diluted at 1:10000. The HRP-conjugated anti-mouset IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-43713_mouse-monoclonal-rfp-antibody-1610-western-blot-26112025196215.jpg)
![Immunoprecipitation: RFP Antibody [NBP2-19302] - RFP antibody Immunoprecipitation of RFP-tagged protein. HeLa transfected lysates were subjected to immunoprecipitation using (B) normal rabbit IgG or (C) 2.5 ug of anti-RFP antibody. (A) Input, 15ug of HeLa transfected lysate. The precipitated protein was detecteddiluted at 1:10000.](http://images.novusbio.com/fullsize/RFP-Antibody-Immunoprecipitation-NBP2-19302-img0003.jpg "Immunoprecipitation: RFP Antibody [NBP2-19302] - RFP antibody Immunoprecipitation of RFP-tagged protein. HeLa transfected lysates were subjected to immunoprecipitation using (B) normal rabbit IgG or (C) 2.5 ug of anti-RFP antibody. (A) Input, 15ug of HeLa transfected lysate. The precipitated protein was detecteddiluted at 1:10000.")
![Western Blot: RFP Antibody [NBP2-19302] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with RFP antibody (NBP2-19302) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-19302_rabbit-polyclonal-rfp-antibody-9102024207593.jpg)
![ELISA: RFP Antibody (RFP40) - VHH - BSA Free [NBP3-12167] - ELISA Results of RFP antibody (RFP40) reactive against RFP. The working dilution for clone RFP40 is 1:31000. Each well was coated in duplicate with 1.0ug of RFP. The starting dilution of antibody was 45 ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% Fish Gel/PBS blocking buffer. Mouse Anti-FLAG Tag HRP conjugated Antibody and TMB substrate.](http://images.novusbio.com/fullsize/RFP-Antibody-RFP40-VHH-BSA-Free-ELISA-NBP3-12167-img0001.jpg "ELISA: RFP Antibody (RFP40) - VHH - BSA Free [NBP3-12167] - ELISA Results of RFP antibody (RFP40) reactive against RFP. The working dilution for clone RFP40 is 1:31000. Each well was coated in duplicate with 1.0ug of RFP. The starting dilution of antibody was 45 ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% Fish Gel/PBS blocking buffer. Mouse Anti-FLAG Tag HRP conjugated Antibody and TMB substrate.")
![Dot Blot: RFP Antibody (RFP40) - VHH - BSA Free [NBP3-12167] - Row A: Recombinant RFP protein. Amount Dotted: (1) 200, (2) 100, (3) 50, (4) 25, (5) 12.5ng. Primary Antibody: 1.0ug/mL for 1.0hr at RT. Secondary Antibody: Mouse Anti-Flag Peroxidase at 1:5000 for 30 mins at RT. Block: BlockOut Buffer.. Exposure: 10 seconds.](http://images.novusbio.com/fullsize/RFP-Antibody-RFP40-VHH-BSA-Free-Dot-Blot-NBP3-12167-img0002.jpg)
![Western Blot Results of RFP Antibody (RFP40) - VHH - BSA Free. Lane 1: Opal Prestained Molecular Weight . Lane 2: HEK293 Whole Cell Lysate [10ug] (-). Lane 3: HEK293 Whole Cell Lysate [10ug] + GFP rec. protein [1ug] (-). Lane 4: HEK293 Whole Cell Lysate [10ug] + RFP rec. protein [1ug] (+). Lane 5: RFP rec. protein [1ug] (+).Primary Antibody: RFP Antibody (RFP40) - VHH - BSA Free at 1:1000 for overnight at 4°C. Secondary Antibody: Mouse Anti-6x His DL649 conjugated at 1:10,000 for 1hr at RT. Block: Blocking Buffer for Fluorescent Western Blotting overnight at 4°C. Expected MW: ~27kDa for RFP protein.](http://images.novusbio.com/fullsize/nbp3-12167_llama-monoclonal-rfp-antibody-rfp40-187202581873.jpg)
![Western Blot: RFP Antibody (HL2028) - Azide and BSA Free [NBP3-25634] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted antibodies as follows: Upper image: RFP antibody [HL2028], NBP3-25634 (1:5000) Middle image: mCherry-tagged protein antibody, NBP2-43720 Lower image: FP-tagged protein antibody, The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25634_rabbit-rfp-mab-hl2028-azide-and-bsa-free-2120249415930.jpg "Western Blot: RFP Antibody (HL2028) - Azide and BSA Free [NBP3-25634] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted antibodies as follows: Upper image: RFP antibody [HL2028], NBP3-25634 (1:5000) Middle image: mCherry-tagged protein antibody, NBP2-43720 Lower image: FP-tagged protein antibody, The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: RFP Antibody (HL2028) - Azide and BSA Free [NBP3-25634] - RFP-tag antibody detects RFP tagged protein by immunofluorescent analysis. Sample: RFP tagged protein transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: RFP tagged protein stained by RFP-tag antibody (NBP3-25634) diluted at 500. Red: RFP tagged protein is expressed in the transfected cells. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25634_rabbit-rfp-mab-hl2028-azide-and-bsa-free-2120249351310.jpg)
![Western Blot: RFP Antibody (1433) [NBP2-43580] - Analysis of A. 30 ug 293T whole cell extract B. 30 ug 293T whole cell extract expressing GFP-tagged protein C. 30 ug 293T whole cell extract expressing RFP-tagged protein 12 % SDS-PAGE RFP antibody dilution: 1:10000.](http://images.novusbio.com/fullsize/RFP-Antibody-1433-Western-Blot-NBP2-43580-img0002.jpg "Western Blot: RFP Antibody (1433) [NBP2-43580] - Analysis of A. 30 ug 293T whole cell extract B. 30 ug 293T whole cell extract expressing GFP-tagged protein C. 30 ug 293T whole cell extract expressing RFP-tagged protein 12 % SDS-PAGE RFP antibody dilution: 1:10000.")
![Immunoprecipitation: RFP Antibody (1433) [NBP2-43580] - Analysis of 400 ug cell lysate/extract of RFP-fused gene transfected 293T cells A. 30 ug lysate/extract of transfected 293T cell B. Control with 2 ug of preimmune mouse IgG C. Immunoprecipitation of RFP-fused protein by 2 ug of RFP tag antibody 12% SDS-PAGE The immunoprecipitated RFP-fused protein was detected by RFP tag antibody diluted at 1:1000. EasyBlot anti-nouse IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/RFP-Antibody-1433-Immunoprecipitation-NBP2-43580-img0001.jpg)
![Western Blot: RFP Antibody [NBP1-45840] - Analysis with recombinant RFP. Lane 1: 10 ng; Lane 2: 50 ng; Lane 3: 250 ng; Lane 4: 1 ug.](http://images.novusbio.com/fullsize/RFP-Antibody-Western-Blot-NBP1-45840-img0002.jpg "Western Blot: RFP Antibody [NBP1-45840] - Analysis with recombinant RFP. Lane 1: 10 ng; Lane 2: 50 ng; Lane 3: 250 ng; Lane 4: 1 ug.")
![Western Blot: TRIM27 Antibody (4D4) [NBP2-46214] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TRIM27.](http://images.novusbio.com/fullsize/TRIM27-Antibody-4D4-Western-Blot-NBP2-46214-img0007.jpg "Western Blot: TRIM27 Antibody (4D4) [NBP2-46214] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TRIM27.")
![Immunohistochemistry: TRIM27 Antibody (4D4) [NBP2-46214] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/TRIM27-Antibody-4D4-Immunohistochemistry-NBP2-46214-img0006.jpg)
![Immunohistochemistry: TRIM27 Antibody (4D4) [NBP2-46214] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/TRIM27-Antibody-4D4-Immunohistochemistry-NBP2-46214-img0001.jpg)
![Immunohistochemistry: TRIM27 Antibody (4D4) [NBP2-46214] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/TRIM27-Antibody-4D4-Immunohistochemistry-NBP2-46214-img0002.jpg)
![Immunohistochemistry: TRIM27 Antibody (4D4) [NBP2-46214] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/TRIM27-Antibody-4D4-Immunohistochemistry-NBP2-46214-img0003.jpg)
![Immunohistochemistry: TRIM27 Antibody (4D4) [NBP2-46214] - Analysis of Carcinoma of Human lung tissue .(Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/TRIM27-Antibody-4D4-Immunohistochemistry-NBP2-46214-img0004.jpg)
![Immunohistochemistry: TRIM27 Antibody (4D4) [NBP2-46214] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/TRIM27-Antibody-4D4-Immunohistochemistry-NBP2-46214-img0005.jpg)