RFP Antibody

Immunoprecipitation: RFP Antibody [NBP2-19302] - RFP antibody Immunoprecipitation of RFP-tagged protein. HeLa transfected lysates were subjected to immunoprecipitation using (B) normal rabbit IgG or (C) 2.5 ug of anti-RFP antibody. (A) Input, 15ug of HeLa transfected lysate. The precipitated protein was detecteddiluted at 1:10000.

Western Blot: RFP Antibody [NBP2-19302] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with RFP antibody (NBP2-19302) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

RFP antibody detects RFP protein at by immunofluorescent analysis.Sample: RFP tagged protein transfected HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: RFP protein stained by RFP antibody (NBP2-19302) diluted at 1:5000.Red: RFP is expressed in the RFP-fusion protein tranfected cell.Blue: Hoechst 33342 staining.The cell of merger panel with no RFP expressed as a negative control.

Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with RFP antibody (NBP2-19302) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: All-NA
• Applications: WB, ICC/IF, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

RFP Antibody Summary

• Description: Novus Biologicals Rabbit RFP Antibody (NBP2-19302) is a polyclonal antibody validated for use in WB, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length RFP recombinant protein
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:5000
  • Immunoprecipitation 1:10000
  • Western Blot 1:5000-1:20000
• Theoretical MW: 27 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for RFP Antibody

• DSRED
• GFP-like chromoprotein
• Red Fluorescent Protein
• RFP

Background

Fluorescent proteins have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

FAQs for RFP Antibody (NBP2-19302). (Showing 1 - 1 of 1 FAQ).

1. Is this antibody (NBP2-19302) also working against tdTomato? I would like to use antibody for Immunohistochemistry or Immunofluorescence.
   • NBP2-19302 has never been tested for cross-reactivity to dsRed derivatives. NBP2-19302 will most likely cross-react though as TdTomato and dsRed are over 90% similar. As you need an antibody for IHC, I do recommend NBP2-19302, as though it has never been tested in tissues, it does work well on cells. You would be eligible for our Innovator's Reward program.