RFP Antibody

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Western Blot: RFP Antibody [NBP1-97371] - Antibody was added at 1.0ug/mL at RT for 2 hours. IRDye800(R) anti-Chicken was added at 1:20,000 at RT for 45 minutes.

Product Details

Summary
Reactivity NASpecies Glossary
Applications WB, ELISA
Clonality
Polyclonal
Host
Chicken
Conjugate
Unconjugated

Order Details

RFP Antibody Summary

Immunogen
The immunogen is a Red Fluorescent Protein (RFP) fusion protein corresponding to the full length amino acid sequence (234aa) derived from the mushroom polyp coral Discosoma.
Specificity
This product has been assayed against 1.0 ug of Biotinylated BSA in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Rabbit) and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Biotin coupled to sepharose beads followed by pepsin digestion and chromatographic separation.
Clonality
Polyclonal
Host
Chicken
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:3000
  • ELISA 1:10000
Application Notes
Polyclonal anti-RFP is designed - detect RFP and its variants. This antibody can be used - detect RFP by ELISA for the direct binding of antigen. Biotin conjugated polyclonal anti-RFP used in a sandwich ELISA with unconjugated anti-RFP is well suited - titrate RFP in solution. The detection antibody conjugated - biotin is subsequently reacted with streptavidin conjugated HRP. Fluorochrome conjugated polyclonal anti-RFP can be used - detect RFP by Immunofluorescence microscopy in cell expression systems and can detect RFP containing inserts. Significant amplification of signal is achieved using fluorochrome conjugated polyclonal anti-RFP relative - the fluorescence of RFP alone. For immunoblotting use either alkaline phosphatase or peroxidase conjugated polyclonal anti-RFP - detect RFP or RFP containing proteins on western blots. This product shows optimal performance by western blot.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for RFP Antibody

  • dsRedGene Symbol
  • Recombinant RFP Protein
  • Red Fluorescent Protein

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for RFP Antibody (NBP1-97371). (Showing 1 - 1 of 1 FAQ).

  1. Would this antibody detect DsRed?
    • NBP1-45840, NBP1-97373 and NBP1-97371 will all recognize DsRed. I have no information about its cross-reactivity of DsRed with NBP1-96752.

Secondary Antibodies

 

Isotype Controls

Additional RFP Products

RFP NBP1-97371

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