RFP Antibody (RFP40) - VHH - BSA Free

ELISA: RFP Antibody (RFP40) - VHH - BSA Free [NBP3-12167] - ELISA Results of RFP antibody (RFP40) reactive against RFP. The working dilution for clone RFP40 is 1:31000. Each well was coated in duplicate with 1.0ug of RFP. The starting dilution of antibody was 45 ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% Fish Gel/PBS blocking buffer. Mouse Anti-FLAG Tag HRP conjugated Antibody and TMB substrate.

Dot Blot: RFP Antibody (RFP40) - VHH - BSA Free [NBP3-12167] - Row A: Recombinant RFP protein. Amount Dotted: (1) 200, (2) 100, (3) 50, (4) 25, (5) 12.5ng. Primary Antibody: 1.0ug/mL for 1.0hr at RT. Secondary Antibody: Mouse Anti-Flag Peroxidase at 1:5000 for 30 mins at RT. Block: BlockOut Buffer.. Exposure: 10 seconds.

Western Blot Results of RFP Antibody (RFP40) - VHH - BSA Free. Lane 1: Opal Prestained Molecular Weight . Lane 2: HEK293 Whole Cell Lysate [10ug] (-). Lane 3: HEK293 Whole Cell Lysate [10ug] + GFP rec. protein [1ug] (-). Lane 4: HEK293 Whole Cell Lysate [10ug] + RFP rec. protein [1ug] (+). Lane 5: RFP rec. protein [1ug] (+).Primary Antibody: RFP Antibody (RFP40) - VHH - BSA Free at 1:1000 for overnight at 4°C. Secondary Antibody: Mouse Anti-6x His DL649 conjugated at 1:10,000 for 1hr at RT. Block: Blocking Buffer for Fluorescent Western Blotting overnight at 4°C. Expected MW: ~27kDa for RFP protein.

• Reactivity: In
• Applications: DB, ELISA, ICC/IF, IHC
• Clone: RFP40
• Clonality: Monoclonal
• Host: Llama
• Conjugate: Unconjugated
• Format: BSA Free

RFP Antibody (RFP40) - VHH - BSA Free Summary

• Description: This recombinant antibody clone was isolated from a library prepared from a hyper-immunized llama host and purified by affinity chromatography from bacterial culture (E. coli)..
  
  Store at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: RFP
• Specificity: Expect reactivity against RFP-labeled probes and proteins.
• Clonality: Monoclonal
• Host: Llama
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Dot Blot 1 ug/ml
  • ELISA 1:31000
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
• Application Notes: This product is a his-tagged monoclonal recombinant antibody designed to detect Red Fluorescent Protein and its conjugates. This antibody has been tested by dot blot and ELISA and is intended for use in immunological assays including ELISA, immunofluorescence and fluorescence activated cell sorting (FACS). The antibody can be labeled with dyes, enzymes or fluorescence, directly or secondarily, for visualization and detection of RFP-conjugated molecules by immunofluorescence. Secondary detection can be achieved using conjugated anti-His tag or anti-VHH antibodies. Optimal titers for applications should be confirmed by the end user. This antibody is not suitable for Western blot detection of denatured RFP.

Reactivity Notes

Sea anemone (Discosoma sp)

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 1X PBS, pH 7.4, 20% (v/v) Glycerol
• Preservative: 0.01% Sodium Azide
• Purity: Affinity purified

Alternate Names for RFP Antibody (RFP40) - VHH - BSA Free

• DSRED
• GFP-like chromoprotein
• Red Fluorescent Protein
• RFP

Background

Fluorescent proteins have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q9U6Y8(Invertebrate)