RFP Antibody (1433) - Azide and BSA Free

Western Blot: RFP Antibody (1433) [NBP2-43580] - Analysis of A. 30 ug 293T whole cell extract B. 30 ug 293T whole cell extract expressing GFP-tagged protein C. 30 ug 293T whole cell extract expressing RFP-tagged protein 12 % SDS-PAGE RFP antibody dilution: 1:10000.

Immunoprecipitation: RFP Antibody (1433) [NBP2-43580] - Analysis of 400 ug cell lysate/extract of RFP-fused gene transfected 293T cells A. 30 ug lysate/extract of transfected 293T cell B. Control with 2 ug of preimmune mouse IgG C. Immunoprecipitation of RFP-fused protein by 2 ug of RFP tag antibody 12% SDS-PAGE The immunoprecipitated RFP-fused protein was detected by RFP tag antibody diluted at 1:1000. EasyBlot anti-nouse IgG was used as a secondary reagent.

• Reactivity: All-NA
• Applications: WB, IP
• Clone: 1433
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

RFP Antibody (1433) - Azide and BSA Free Summary

• Immunogen: Recombinant protein conjugated synthetic peptide encompassing a sequence within the center region of RFP. The exact sequence is proprietary.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 1:100-1:500
  • Western Blot 1:5000-1:20000
• Theoretical MW: 27 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein G purified

Alternate Names for RFP Antibody (1433) - Azide and BSA Free

• DSRED
• GFP-like chromoprotein
• Red Fluorescent Protein
• RFP

Background

Fluorescent proteins have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.