RFP Antibody (1433)

Western Blot: RFP Antibody (1433) [NBP2-43580] - Analysis of A. 30 ug 293T whole cell extract B. 30 ug 293T whole cell extract expressing GFP-tagged protein C. 30 ug 293T whole cell extract expressing RFP-tagged protein 12 % SDS-PAGE RFP antibody dilution: 1:10000.

Immunoprecipitation: RFP Antibody (1433) [NBP2-43580] - Analysis of 400 ug cell lysate/extract of RFP-fused gene transfected 293T cells A. 30 ug lysate/extract of transfected 293T cell B. Control with 2 ug of preimmune mouse IgG C. Immunoprecipitation of RFP-fused protein by 2 ug of RFP tag antibody 12% SDS-PAGE The immunoprecipitated RFP-fused protein was detected by RFP tag antibody diluted at 1:1000. EasyBlot anti-nouse IgG was used as a secondary reagent.

• Reactivity: All-NA
• Applications: WB, IP
• Clone: 1433
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

RFP Antibody (1433) Summary

• Immunogen: Recombinant protein conjugated synthetic peptide encompassing a sequence within the center region of RFP. The exact sequence is proprietary.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 1:100-1:500
  • Western Blot 1:5000-1:20000
• Theoretical MW: 27 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein G purified

Alternate Names for RFP Antibody (1433)

• dsRedGene Symbol
• Recombinant RFP Protein
• Red Fluorescent Protein

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.