IL-2 Proteins

BT-002). It has equivalent bioactivity to Recombinant Human IL-2 (Catalog # 202-IL). It combines R&D Systems quality with scalability that allows for a solid supply chain. Both Recombinant Human IL-2 proteins are measured in a cell proliferation assay using CTLL 2 mouse cytotoxic T cells." class="big_lightbox"> BT-002). It has equivalent bioactivity to Recombinant Human IL-2 (Catalog # 202-IL). It combines R&D Systems quality with scalability that allows for a solid supply chain. Both Recombinant Human IL-2 proteins are measured in a cell proliferation assay using CTLL 2 mouse cytotoxic T cells." alt="As an alternative, please consider our next generation Recombinant Human IL-2 (BT-002). It has equivalent bioactivity to Recombinant Human IL-2 (Catalog # 202-IL). It combines R&D Systems quality with scalability that allows for a solid supply chain. Both Recombinant Human IL-2 proteins are measured in a cell proliferation assay using CTLL 2 mouse cytotoxic T cells." class="big_thumb" />

BT-002HS) Provides consistent CD4+/CD8+ T Cell Proliferation in Side-by-Side Testing with the Standard IL-2 Protein. CD4+ and CD8+ T cells were activated with anti-CD3- , anti-CD28- conjugated beads, and grown for 14 days in media containing 10 ng/mL Animal-free Recombinant Human IL-2 (R&D Systems, Catalog # BT-002-AFL) as the standard IL-2 protein or the AI modified Heat Stable IL-2. IL-2 conditions were refreshed every 2-3 days. The average fold expansion of the cells was determined every 2-3 days and showed that the Heat Stable IL-2 protein performs equally compared to the standard IL-2 protein." class="big_lightbox"> BT-002HS) Provides consistent CD4+/CD8+ T Cell Proliferation in Side-by-Side Testing with the Standard IL-2 Protein. CD4+ and CD8+ T cells were activated with anti-CD3- , anti-CD28- conjugated beads, and grown for 14 days in media containing 10 ng/mL Animal-free Recombinant Human IL-2 (R&D Systems, Catalog # BT-002-AFL) as the standard IL-2 protein or the AI modified Heat Stable IL-2. IL-2 conditions were refreshed every 2-3 days. The average fold expansion of the cells was determined every 2-3 days and showed that the Heat Stable IL-2 protein performs equally compared to the standard IL-2 protein." class="big_thumb" />

BT-002-AFL) as the standard IL-2 protein or Heat Stable IL-2 (Cat # BT-002HS). IL-2 conditions were refreshed every 2-3 days. After 21 days, the phenotypes of the cells were compared by flow cytometry using a PE-Cy7-conjugated mouse anti-human CD45RA monoclonal antibody and a Brilliant Violet 711 conjugated mouse anti-human CCR7 monoclonal antibody to determine the number of CD4+ and CD8+ naive (CD45RA+ CCR7+), central memory (CD45RA- CCR7+), terminal effector memory (CD45RA+ CCR7- ), and effector memory (CD45RA- CCR7- ) T cells. The percentages of each of these cell populations were found to be similar whether the cells were expanded with the standard or Heat Stable IL-2 protein." class="big_lightbox"> BT-002-AFL) as the standard IL-2 protein or Heat Stable IL-2 (Cat # BT-002HS). IL-2 conditions were refreshed every 2-3 days. After 21 days, the phenotypes of the cells were compared by flow cytometry using a PE-Cy7-conjugated mouse anti-human CD45RA monoclonal antibody and a Brilliant Violet 711 conjugated mouse anti-human CCR7 monoclonal antibody to determine the number of CD4+ and CD8+ naive (CD45RA+ CCR7+), central memory (CD45RA- CCR7+), terminal effector memory (CD45RA+ CCR7- ), and effector memory (CD45RA- CCR7- ) T cells. The percentages of each of these cell populations were found to be similar whether the cells were expanded with the standard or Heat Stable IL-2 protein." class="big_thumb" />

BT-002-AFL) as the standard IL-2 protein or engineered Recombinant Human IL-2 (CD122-Directed) Protein (BT-002DBR), refreshed every 2-3 days. The average fold expansion of the cells was determined every 2-3 days. The IL-2 (CD122-Directed) Protein supports greater than 6x more CD4+/CD8+ T cell growth than the standard IL-2 protein." class="big_lightbox"> BT-002-AFL) as the standard IL-2 protein or engineered Recombinant Human IL-2 (CD122-Directed) Protein (BT-002DBR), refreshed every 2-3 days. The average fold expansion of the cells was determined every 2-3 days. The IL-2 (CD122-Directed) Protein supports greater than 6x more CD4+/CD8+ T cell growth than the standard IL-2 protein." alt="CD4⁺ and CD8⁺ T cells from two different donors were activated with anti-CD3-, anti-CD28-conjugated beads, and grown for 28 days in media containing either 3.6 or 14.3 ng/mL of Animal-free Recombinant Human IL-2 Protein (BT-002-AFL) as the standard IL-2 protein or engineered Recombinant Human IL-2 (CD122-Directed) Protein (BT-002DBR), refreshed every 2-3 days. The average fold expansion of the cells was determined every 2-3 days. The IL-2 (CD122-Directed) Protein supports greater than 6x more CD4+/CD8+ T cell growth than the standard IL-2 protein." class="big_thumb" />

BT-002-AFL) as the standard IL-2 protein or Recombinant Human IL-2 (CD122-Directed) Protein (BT-002DBR), refreshed every 2-3 days. A) After 28 days, the phenotypes of the cells were compared by flow cytometry using a Brilliant Violet 711™ anti-human CD197 (CCR7) antibody and PE/Dazzle™ 594 anti-human CD45RA antibody to determine the number of CD4⁺ and CD8⁺ naive (CD45RA⁺/CCR7⁺) Tscm cells. The percentage of Tscm cells was found to be higher when the cells were expanded with the IL-2 (CD122-Directed) Protein at 14.3 ng/mL. B) The number of cells expressing the T cell exhaustion markers, PD-1, LAG-3, and TIM-3 was determined by flow cytometry. The results showed that the percentages of CD4⁺/CD8⁺ T cells that were also PD-1, LAG-3, or TIM-3 positive were equivalent or reduced with the IL-2 (CD122-Directed) Protein. These results demonstrate that the IL-2 (CD122-Directed) Protein promotes increased T cell expansion with enhanced expression of naive phenotype markers and equivalent or reduced expression of cell exhaustion markers. " class="big_lightbox"> BT-002-AFL) as the standard IL-2 protein or Recombinant Human IL-2 (CD122-Directed) Protein (BT-002DBR), refreshed every 2-3 days. A) After 28 days, the phenotypes of the cells were compared by flow cytometry using a Brilliant Violet 711™ anti-human CD197 (CCR7) antibody and PE/Dazzle™ 594 anti-human CD45RA antibody to determine the number of CD4⁺ and CD8⁺ naive (CD45RA⁺/CCR7⁺) Tscm cells. The percentage of Tscm cells was found to be higher when the cells were expanded with the IL-2 (CD122-Directed) Protein at 14.3 ng/mL. B) The number of cells expressing the T cell exhaustion markers, PD-1, LAG-3, and TIM-3 was determined by flow cytometry. The results showed that the percentages of CD4⁺/CD8⁺ T cells that were also PD-1, LAG-3, or TIM-3 positive were equivalent or reduced with the IL-2 (CD122-Directed) Protein. These results demonstrate that the IL-2 (CD122-Directed) Protein promotes increased T cell expansion with enhanced expression of naive phenotype markers and equivalent or reduced expression of cell exhaustion markers. " class="big_thumb" />

10919-2B) was captured on a Sensor Chip Protein AGL, and binding to Recombinant Human IL-2 (CD122-Directed) Protein (BT-002DBR) was measured at a concentration range between 0.097nM and 200 nM. The double-referenced sensorgram was fit to a 1:1 binding model to determine the binding kinetics and affinity, with an affinity constant of K_d=49.3 nM. Note: The IL-2 (CD122-Directed) Protein was modeled to engage only the IL-2 dimeric beta gamma receptor complex. In comparison, wild type recombinant human IL-2 (BT-002) affinity to Recombinant Human IL 2 R beta Fc Chimera (10919-2B) has a binding constant K_d=544 nm." class="big_lightbox"> 10919-2B) was captured on a Sensor Chip Protein AGL, and binding to Recombinant Human IL-2 (CD122-Directed) Protein (BT-002DBR) was measured at a concentration range between 0.097nM and 200 nM. The double-referenced sensorgram was fit to a 1:1 binding model to determine the binding kinetics and affinity, with an affinity constant of K_d=49.3 nM. Note: The IL-2 (CD122-Directed) Protein was modeled to engage only the IL-2 dimeric beta gamma receptor complex. In comparison, wild type recombinant human IL-2 (BT-002) affinity to Recombinant Human IL 2 R beta Fc Chimera (10919-2B) has a binding constant K_d=544 nm." class="big_thumb" />

10453-IL) stimulates cell proliferation of the CTLL-2 mouse cytotoxic T cell line. The ED₅₀ for this effect is 0.05-0.3 ng/mL. Mammalian-expressed Recombinant Human IL-2 has similar activity to E.coli-derived Recombinant Human IL-2 (202-IL)." class="big_lightbox"> 10453-IL) stimulates cell proliferation of the CTLL-2 mouse cytotoxic T cell line. The ED₅₀ for this effect is 0.05-0.3 ng/mL. Mammalian-expressed Recombinant Human IL-2 has similar activity to E.coli-derived Recombinant Human IL-2 (202-IL)." class="big_thumb" />

10453-IL) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 14-18 kDa." class="big_lightbox"> 10453-IL) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 14-18 kDa." class="big_thumb" />

PPK-002-GMP) stimulates proliferation of CTLL‑2 mouse cytotoxic T cells. The ED₅₀ for this effect is 0.0300-0.250 ng/mL. Three independent lots were tested for activity and plotted on the same graph to show lot-to-lot consistency of ProPak™ GMP IL-2." class="big_lightbox"> PPK-002-GMP) stimulates proliferation of CTLL‑2 mouse cytotoxic T cells. The ED₅₀ for this effect is 0.0300-0.250 ng/mL. Three independent lots were tested for activity and plotted on the same graph to show lot-to-lot consistency of ProPak™ GMP IL-2." alt="ProPak™ GMP-grade Recombinant Human IL-2 (PPK-002-GMP) stimulates proliferation of CTLL‑2 mouse cytotoxic T cells. The ED₅₀ for this effect is 0.0300-0.250 ng/mL. Three independent lots were tested for activity and plotted on the same graph to show lot-to-lot consistency of ProPak™ GMP IL-2." class="big_thumb" />

BT-002-GMP) was added to Xeno-free Human T cell media in a bottle (CCM038-GMP-1L). After cytokine addition, the concentration of IL-2 (SPCKB-PS-000295) was determined by the Ella automated immunoassay system. Data is the average of 4 independent experiments with error bars ±SD. " class="big_lightbox"> BT-002-GMP) was added to Xeno-free Human T cell media in a bottle (CCM038-GMP-1L). After cytokine addition, the concentration of IL-2 (SPCKB-PS-000295) was determined by the Ella automated immunoassay system. Data is the average of 4 independent experiments with error bars ±SD. " class="big_thumb" />

BT-002-GMP) was added to Xeno-free Human T cell media in a bottle (CCM038-GMP-1L). T cells from 3 donors were activated and expanded in G-Rex bioreactors for 7 days and assessed for cells/cm² and cell viability. Data is the average of 4 independent experiments with 3 donors per experiment, error bars ±SD. " class="big_lightbox"> BT-002-GMP) was added to Xeno-free Human T cell media in a bottle (CCM038-GMP-1L). T cells from 3 donors were activated and expanded in G-Rex bioreactors for 7 days and assessed for cells/cm² and cell viability. Data is the average of 4 independent experiments with 3 donors per experiment, error bars ±SD. " class="big_thumb" />

BT-002-GMP) was added to Xeno-free Human T cell media in a bottle (CCM038-GMP-1L). T cells from 3 donors were activated for 1 day then transduced with eF1a-CD19-DHFR-eGFP lentivirus. Cells were expanded in a G-Rex® bioreactor for a total of 7 days, then assessed for fold expansion and cell viability. Data is the average of 3 donors, error bars ±SD. " class="big_lightbox"> BT-002-GMP) was added to Xeno-free Human T cell media in a bottle (CCM038-GMP-1L). T cells from 3 donors were activated for 1 day then transduced with eF1a-CD19-DHFR-eGFP lentivirus. Cells were expanded in a G-Rex® bioreactor for a total of 7 days, then assessed for fold expansion and cell viability. Data is the average of 3 donors, error bars ±SD. " class="big_thumb" />

BT-002-GMP) Animal-Free (Catalog # BT-002-AFL) and RUO (BT-002) grades of Recombinant Human IL-2 as measured in a cell proliferation assay using CTLL-2 mouse cytotoxic T cells (orange, green, red, respectively)." class="big_lightbox"> BT-002-GMP) Animal-Free (Catalog # BT-002-AFL) and RUO (BT-002) grades of Recombinant Human IL-2 as measured in a cell proliferation assay using CTLL-2 mouse cytotoxic T cells (orange, green, red, respectively)." class="big_thumb" />

1020-RL) is coated at 1 µg/mL, Recombinant Biotinylated Human IL-2 (Catalog # BT202) binds with an ED₅₀ of 4-24 ng/mL." class="big_lightbox"> 1020-RL) is coated at 1 µg/mL, Recombinant Biotinylated Human IL-2 (Catalog # BT202) binds with an ED₅₀ of 4-24 ng/mL." alt="When Recombinant Human IL-2 Ra Fc Chimera (Catalog # 1020-RL) is coated at 1 µg/mL, Recombinant Biotinylated Human IL-2 (Catalog # BT202) binds with an ED₅₀ of 4-24 ng/mL." class="big_thumb" />

BT202, 500 ng/mL) binds to HEK293 human embryonic kidney cell line transfected with recombinant human CD25/IL-2R alpha (black dotted line). Binding is completely blocked (orange histogram) by 0.5 µg/mL of Mouse Anti-Human CD25/IL-2R alpha Monoclonal Antibody (Catalog # MAB1020). Mouse IgG2A Isotype Control (Catalog # MAB003) at 0.5 µg/mL was used as a control (blue line). Ligand binding was detected by staining cells with APC-conjugated Streptavidin (Catalog # F0050)." class="big_lightbox"> BT202, 500 ng/mL) binds to HEK293 human embryonic kidney cell line transfected with recombinant human CD25/IL-2R alpha (black dotted line). Binding is completely blocked (orange histogram) by 0.5 µg/mL of Mouse Anti-Human CD25/IL-2R alpha Monoclonal Antibody (Catalog # MAB1020). Mouse IgG2A Isotype Control (Catalog # MAB003) at 0.5 µg/mL was used as a control (blue line). Ligand binding was detected by staining cells with APC-conjugated Streptavidin (Catalog # F0050)." class="big_thumb" />