HAF109). Specific bands were detected for Host Cell Factor 1/HCFC1 at approximately 300 kDa (as indicated) and proteolytic fragments of Host Cell Factor 1/HCFC1 at approximately 100 to 175 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and Daudi human Burkitt's lymphoma cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human Host Cell Factor 1/HCFC1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6254) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). Specific bands were detected for Host Cell Factor 1/HCFC1 at approximately 300 kDa (as indicated) and proteolytic fragments of Host Cell Factor 1/HCFC1 at approximately 100 to 175 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF6254-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-03391-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-31788-25ul | |||
![Western Blot: Host Cell Factor 1/HCFC1 Antibody [NB100-68209] - Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-HCF1 antibody NB100-68209 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. HCF1 was also immunoprecipitated by rabbit anti-HCF1 antibody NB100-68210, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).](http://images.novusbio.com/fullsize/Host-Cell-Factor-1-HCFC1-Antibody-Western-Blot-NB100-68209-img0010.jpg "<b>Independent Antibodies Validation. </b>Western Blot: Host Cell Factor 1/HCFC1 Antibody [NB100-68209] - Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-HCF1 antibody NB100-68209 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. HCF1 was also immunoprecipitated by rabbit anti-HCF1 antibody NB100-68210, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).")
![Western Blot: Host Cell Factor 1/HCFC1 Antibody [NB100-68209] - 5-FU-produced ROS involved in histone methyltransferase-mediated Nrf2 expression. E. Expression patterns of TET1, OGT, HCF1, & MLL in NAC pre-treated SNUC5/5-FUR cells determined by WB analysis. *Significantly different from SNUC5 cells (p<0.05), & #significantly different from SNUC5/5-FUR cells (p<0.05). Also, SNUC5 cells pre-treated with 500 μM of NAC & incubated for an additional 1 h at 37°. Cells then treated with 140 μM 5-FU for 48 h. 10 μM of DHR123 was added to plate & incubated for an additional 30 min at 37°C. Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.9745), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-68209_rabbit-polyclonal-host-cell-factor-1-hcfc1-antibody-310202415171343.jpg)
![Western Blot: Host Cell Factor 1/HCFC1 Antibody [NB100-68209] - 5-FU-produced ROS involved in histone methyltransferase-mediated Nrf2 expression. J. Expression of TET1 & interaction proteins detected by WB analysis. *Significantly different from SNUC5 cells (p<0.05), & #significantly different from 5-FU-treated SNUC5 cells (p<0.05). Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.9745), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-68209_rabbit-polyclonal-host-cell-factor-1-hcfc1-antibody-310202415345312.jpg)
![Western Blot: Host Cell Factor 1/HCFC1 Antibody [NB100-68209] - 5-FU-produced ROS involved in histone methyltransferase-mediated Nrf2 expression. F. The intracellular ROS level was detected by flow cytometry. Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.9745), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-68209_rabbit-polyclonal-host-cell-factor-1-hcfc1-antibody-31020241534395.jpg)
![Western Blot: Host Cell Factor 1/HCFC1 Antibody [NB100-68209] - Effect of MLL-HCF1 interaction on histone methylation & Nrf2 expression in SNUC5/5-FUR cellsA. HCF1 expression was determined by Western blotting. *Significantly different from SNUC5 cells (p<0.05). B. Interaction between MLL & HCF1was examined by immunoprecipitation analysis using anti-MLL & anti-HCF1 antibodies followed by Western blotting with anti-HCF1 & anti-MLL antibodies. *Significantly different from SNUC5 cells (p<0.05). C. Interaction between MLL & HCF1was assessed by PLA. Each green spot represents for a single interaction (MLL & HCF1) & DNA was stained with DAPI. *Significantly different from SNUC5 cells (p<0.05). The cells were transfected with non-targeting siRNA (siControl) or siHCF1 RNA for 24 h. D. Expression pattern of MLL mRNA in SNUC5/5-FUR cells was determined by RT-PCR analysis. *Significantly different from siControl-transfected cells (p<0.05). E. Expression pattern of HCF1, MLL, & Nrf2 in SNUC5/5-FUR cells was determined by Western blot analysis. *Significantly different from siControl-transfected cells (p<0.05). Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.9745), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-68209_rabbit-polyclonal-host-cell-factor-1-hcfc1-antibody-310202415532061.jpg)
![Western Blot: Host Cell Factor 1/HCFC1 Antibody [NB100-68209] - Relation of OGT-TET1 interaction on histone methylation & Nrf2 expression in SNUC5/5-FUR cellsA. Interaction between OGT & TET1 was examined by immunoprecipitation analyses using anti-OGT & anti-TET1 antibodies followed by Western blotting with anti-TET1 & anti-OGT antibodies. B. Interaction between OGT & TET1 was assessed by using PLA. Each green spot represents for a single interaction (OGT & TET1) & DNA was stained with DAPI. *Significantly different from SNUC5 cells (p<0.05). C. The O-GlcNAcylated TET1 or O-GlcNAcylated OGT was examined by immunoprecipitation analyses using anti-O-GlcNAc antibody followed by Western blotting with anti-TET1 & anti-OGT antibodies. The cells were transfected with non-targeting siRNA (siControl) or siOGT RNA or siTET1 RNA for 24 h. D. Expression pattern of OGT & TET1 in SNUC5/5-FUR cells was determined by Western blot analysis. *Significantly different from siControl-transfected cells (p<0.05). E. Expression pattern of TET1, OGT, HCF1, MLL, & Nrf2 in SNUC5/5-FUR cells was determined by Western blot analysis. *Significantly different from siControl-transfected cells (p<0.05). Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.9745), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-68209_rabbit-polyclonal-host-cell-factor-1-hcfc1-antibody-310202415523922.jpg)
![Western Blot: Host Cell Factor 1/HCFC1 Antibody [NB100-68209] - Involvement of OGT in histone methylase MLL/COMPASS-like complex-mediated Nrf2 expression in SNUC5/5-FUR cellsA. OGT & O-GlcNAc expression was determined by Western blotting. *Significantly different from SNUC5 cells (p<0.05). B. Interaction between HCF1 & OGT was examined by immunoprecipitation analysis using anti-HCF1 & anti-OGT antibodies followed by Western blotting with anti-HCF1 & anti-OGT antibodies. C. Interaction between HCF1 & OGT was assessed by using PLA. Each green spot represents for a single interaction (HCF1 & OGT) & DNA was stained with DAPI. *Significantly different from SNUC5 cells (p<0.05). D. Interaction between MLL & OGT was examined by immunoprecipitation analysis using anti-MLL & anti-OGT antibodies followed by Western blotting with anti-OGT & anti-MLL antibodies. E. Interaction between MLL & OGT was assessed by using PLA. Each green spot represents for a single interaction (MLL & OGT) & DNA was stained with DAPI. *Significantly different from SNUC5 cells (p<0.05). The cells were transfected with non-targeting siRNA (siControl) or siOGT RNA for 24 h. F. Expression pattern of MLL mRNA in SNUC5/5-FUR cells was determined by RT-PCR analysis. *Significantly different from siControl-transfected cells (p<0.05). G. Expression pattern of OGT, MLL, HFC1, & Nrf2 in SNUC5/5-FUR cells was determined by Western blot analysis. *Significantly different from siControl-transfected cells (p<0.05). Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.9745), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-68209_rabbit-polyclonal-host-cell-factor-1-hcfc1-antibody-310202415532084.jpg)
![Western Blot: Host Cell Factor 1/HCFC1 Antibody [NB100-68209] - Involvement of OGT in histone methylase MLL/COMPASS-like complex-mediated Nrf2 expression in SNUC5/5-FUR cellsA. OGT & O-GlcNAc expression was determined by Western blotting. *Significantly different from SNUC5 cells (p<0.05). B. Interaction between HCF1 & OGT was examined by immunoprecipitation analysis using anti-HCF1 & anti-OGT antibodies followed by Western blotting with anti-HCF1 & anti-OGT antibodies. C. Interaction between HCF1 & OGT was assessed by using PLA. Each green spot represents for a single interaction (HCF1 & OGT) & DNA was stained with DAPI. *Significantly different from SNUC5 cells (p<0.05). D. Interaction between MLL & OGT was examined by immunoprecipitation analysis using anti-MLL & anti-OGT antibodies followed by Western blotting with anti-OGT & anti-MLL antibodies. E. Interaction between MLL & OGT was assessed by using PLA. Each green spot represents for a single interaction (MLL & OGT) & DNA was stained with DAPI. *Significantly different from SNUC5 cells (p<0.05). The cells were transfected with non-targeting siRNA (siControl) or siOGT RNA for 24 h. F. Expression pattern of MLL mRNA in SNUC5/5-FUR cells was determined by RT-PCR analysis. *Significantly different from siControl-transfected cells (p<0.05). G. Expression pattern of OGT, MLL, HFC1, & Nrf2 in SNUC5/5-FUR cells was determined by Western blot analysis. *Significantly different from siControl-transfected cells (p<0.05). Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.9745), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-68209_rabbit-polyclonal-host-cell-factor-1-hcfc1-antibody-310202415525241.jpg)
![Western Blot: Host Cell Factor 1/HCFC1 Antibody [NB100-68209] - Effect of MLL-HCF1 interaction on histone methylation & Nrf2 expression in SNUC5/5-FUR cellsA. HCF1 expression was determined by Western blotting. *Significantly different from SNUC5 cells (p<0.05). B. Interaction between MLL & HCF1was examined by immunoprecipitation analysis using anti-MLL & anti-HCF1 antibodies followed by Western blotting with anti-HCF1 & anti-MLL antibodies. *Significantly different from SNUC5 cells (p<0.05). C. Interaction between MLL & HCF1was assessed by PLA. Each green spot represents for a single interaction (MLL & HCF1) & DNA was stained with DAPI. *Significantly different from SNUC5 cells (p<0.05). The cells were transfected with non-targeting siRNA (siControl) or siHCF1 RNA for 24 h. D. Expression pattern of MLL mRNA in SNUC5/5-FUR cells was determined by RT-PCR analysis. *Significantly different from siControl-transfected cells (p<0.05). E. Expression pattern of HCF1, MLL, & Nrf2 in SNUC5/5-FUR cells was determined by Western blot analysis. *Significantly different from siControl-transfected cells (p<0.05). Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.9745), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-68209_rabbit-polyclonal-host-cell-factor-1-hcfc1-antibody-310202415523923.jpg)
![Western Blot: Host Cell Factor 1/HCFC1 Antibody [NB100-68209] - Effect of MLL-HCF1 interaction on histone methylation & Nrf2 expression in SNUC5/5-FUR cellsA. HCF1 expression was determined by Western blotting. *Significantly different from SNUC5 cells (p<0.05). B. Interaction between MLL & HCF1was examined by immunoprecipitation analysis using anti-MLL & anti-HCF1 antibodies followed by Western blotting with anti-HCF1 & anti-MLL antibodies. *Significantly different from SNUC5 cells (p<0.05). C. Interaction between MLL & HCF1was assessed by PLA. Each green spot represents for a single interaction (MLL & HCF1) & DNA was stained with DAPI. *Significantly different from SNUC5 cells (p<0.05). The cells were transfected with non-targeting siRNA (siControl) or siHCF1 RNA for 24 h. D. Expression pattern of MLL mRNA in SNUC5/5-FUR cells was determined by RT-PCR analysis. *Significantly different from siControl-transfected cells (p<0.05). E. Expression pattern of HCF1, MLL, & Nrf2 in SNUC5/5-FUR cells was determined by Western blot analysis. *Significantly different from siControl-transfected cells (p<0.05). Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.9745), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-68209_rabbit-polyclonal-host-cell-factor-1-hcfc1-antibody-310202415533831.jpg)
![Western Blot: Host Cell Factor 1/HCFC1 Antibody [NB100-68210] - Detection of Human HCF1 on HeLa whole cell lysate using 100-68210. HCF1 was also immunoprecipitated by rabbit anti-HCF1 antibody NB100-68209.](http://images.novusbio.com/fullsize/Host-Cell-Factor-1-HCFC1-Antibody-Western-Blot-NB100-68210-img0004.jpg "<b>Independent Antibodies Validation. </b>Western Blot: Host Cell Factor 1/HCFC1 Antibody [NB100-68210] - Detection of Human HCF1 on HeLa whole cell lysate using 100-68210. HCF1 was also immunoprecipitated by rabbit anti-HCF1 antibody NB100-68209.")
![Western Blot: Host Cell Factor 1/HCFC1 Antibody [NB100-68210] - Transcriptional activation by E2F4 in mouse ES cells is mediated by chromatin modifiers. a Schematic representation of the affinity purification-mass spectrometry approach to identify the E2F4 interactome. b Silver stain of eluted fractions from RPE cells and mouse ES cells . Slices of gel were subjected to mass spectrometry, & the indicated protein bands were identified by analyzing the proteins enriched in each slice. c GO terms for cellular components enriched in the list of mouse ES cell-specific candidate interactors. d Validation of interactions between GFP-E2F4 & DP-1, HCFC1, YEATS2, LIN54, & LIN9, by co-immunoprecipitation followed by immunoassay, in mouse ES cells & human RPE cells. Transcriptional activators (HCFC1 & YEATS2) preferentially bind to E2F4 in mouse ES cells while members of the DREAM repressor complex (LIN54 & LIN9) bind preferentially to E2F4 in RPE cells. Molecular weights (kDa) are indicated on the left side (one experiment shown of at least two experiments) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31270324), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-68210_rabbit-polyclonal-host-cell-factor-1-hcfc1-antibody-310202415541972.jpg)
NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for 
CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei of epithelial cells. View our protocol for ![Western Blot: Host Cell Factor 1/HCFC1 Antibody [NBP2-16786] - A549 whole cell and nuclear extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with HCF1 antibody [N1], N-term diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Host-Cell-Factor-1-HCFC1-Antibody-Western-Blot-NBP2-16786-img0005.jpg "Western Blot: Host Cell Factor 1/HCFC1 Antibody [NBP2-16786] - A549 whole cell and nuclear extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with HCF1 antibody [N1], N-term diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Western Blot: Host Cell Factor 1/HCFC1 Antibody [NBP2-16786] - Sample (30 ug of whole cell lysate) A: HeLa 5% SDS PAGE gel, diluted at 1:500.](http://images.novusbio.com/fullsize/HCFC1-Antibody-Western-Blot-NBP2-16786-img0001.jpg)
![Rat tissue extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with HCF1 antibody [N1], N-term (NBP2-16786) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-16786_rabbit-polyclonal-host-cell-factor-1-hcfc1-antibody-western-blot-2611202518454019.jpg)
![Western Blot: Host Cell Factor 1/HCFC1 Antibody [NBP3-03391] - Analysis of extracts of various cell lines, using Host Cell Factor 1/HCFC1 antibody at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.](http://images.novusbio.com/fullsize/Host-Cell-Factor-1-HCFC1-Antibody-Western-Blot-NBP3-03391-img0001.jpg "Western Blot: Host Cell Factor 1/HCFC1 Antibody [NBP3-03391] - Analysis of extracts of various cell lines, using Host Cell Factor 1/HCFC1 antibody at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.")
![Immunocytochemistry/Immunofluorescence: HCFC1R1 Antibody [NBP2-31788] - Staining of human cell line A549 shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/HCFC1R1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-31788-img0007.jpg "Immunocytochemistry/Immunofluorescence: HCFC1R1 Antibody [NBP2-31788] - Staining of human cell line A549 shows localization to nucleoplasm.")
![Immunohistochemistry-Paraffin: HCFC1R1 Antibody [NBP2-31788] - Staining of human cerebellum shows moderate cytoplasmic and nuclear positivity in Purkinje cells.](http://images.novusbio.com/fullsize/HCFC1R1-Antibody-Immunohistochemistry-Paraffin-NBP2-31788-img0008.jpg)
![Western Blot: HCFC1R1 Antibody [NBP1-53070] - Human Spleen lysate, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/HCFC1R1-Antibody-Western-Blot-NBP1-53070-img0002.jpg "Western Blot: HCFC1R1 Antibody [NBP1-53070] - Human Spleen lysate, concentration 0.2-1 ug/ml.")
![Western Blot: HCFC1R1 Overexpression Lysate (Adult Normal) [NBP2-09087] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for HCFC1R1.](http://images.novusbio.com/fullsize/HCFC1R1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-09087-img0001.jpg "Western Blot: HCFC1R1 Overexpression Lysate (Adult Normal) [NBP2-09087] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for HCFC1R1.")
![Western Blot: HCFC1R1 Overexpression Lysate (Adult Normal) [NBP2-08973] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for HCFC1R1.](http://images.novusbio.com/fullsize/HCFC1R1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-08973-img0001.jpg "Western Blot: HCFC1R1 Overexpression Lysate (Adult Normal) [NBP2-08973] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for HCFC1R1.")
![Western Blot: HCFC1R1 Overexpression Lysate (Adult Normal) [NBL1-11468] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for HCFC1R1.](http://images.novusbio.com/fullsize/HCFC1R1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-11468-img0002.jpg "Western Blot: HCFC1R1 Overexpression Lysate (Adult Normal) [NBL1-11468] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for HCFC1R1.")
