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![Western Blot: CPN1 Antibody [NBP3-32212] - Western blot analysis of CPN1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32212, 1/500) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate Lane 2: Raji cell lysate Lane 3: Mouse pancreas tissue lysate Predicted band size: 52 kDa Observed band size: 55 kDa](http://images.novusbio.com/fullsize/nbp3-32212_rabbit-cpn1-pab-27820241631630.jpg "Western Blot: CPN1 Antibody [NBP3-32212] - Western blot analysis of CPN1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32212, 1/500) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate Lane 2: Raji cell lysate Lane 3: Mouse pancreas tissue lysate Predicted band size: 52 kDa Observed band size: 55 kDa")
![Immunocytochemistry/ Immunofluorescence: CPN1 Antibody [NBP3-32212] - ICC staining of CPN1 in LOVO cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (NBP3-32212, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32212_rabbit-cpn1-pab-29202410334058.jpg)
![Western Blot: CPN1 Antibody [NBP1-57960] - Transfected 293T, Antibody Titration: 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/CPN1-Antibody-Western-Blot-NBP1-57960-img0001.jpg "Western Blot: CPN1 Antibody [NBP1-57960] - Transfected 293T, Antibody Titration: 0.2-1 ug/ml.")
![Immunohistochemistry-Paraffin: CPN1 Antibody [NBP1-57960] - Human Heart Tissue, antibody concentration 2.5 ug/ml.](http://images.novusbio.com/fullsize/CPN1-Antibody-Immunohistochemistry-Paraffin-NBP1-57960-img0003.jpg)
![Western Blot: CPN1 Antibody - BSA Free [NBP3-47482] - 293T cells were subjected to SDS PAGE followed by western blot with (CPN1 antibody) at dilution of 1:1000](http://images.novusbio.com/fullsize/nbp3-47482_rabbit-cpn1-pab-bsa-free-284202512365467.jpg "Western Blot: CPN1 Antibody - BSA Free [NBP3-47482] - 293T cells were subjected to SDS PAGE followed by western blot with (CPN1 antibody) at dilution of 1:1000")
![Immunohistochemistry: CPN1 Antibody - BSA Free [NBP3-47482] - Immunohistochemistry of paraffin-embedded human liver cancer using (CPN1 antibody) at dilution of 1:200](http://images.novusbio.com/fullsize/nbp3-47482_rabbit-cpn1-pab-bsa-free-28420251259309.jpg)
![HepG2 whole cell extract and conditioned medium (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CPN1 antibody [N1C3] (NBP3-48455) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48455_rabbit-cpn1-pab-western-blot-141120251833459.jpg "<b>Orthogonal Strategies Validation. </b>HepG2 whole cell extract and conditioned medium (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CPN1 antibody [N1C3] (NBP3-48455) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: CPN1 Antibody [NBP1-58016] - Transfected 293T cell lysate, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/CPN1-Antibody-Western-Blot-NBP1-58016-img0002.jpg "Western Blot: CPN1 Antibody [NBP1-58016] - Transfected 293T cell lysate, concentration 0.2-1 ug/ml.")
![Western Blot: CPN1 Overexpression Lysate (Adult Normal) [NBL1-09440] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CPN1.](http://images.novusbio.com/fullsize/CPN1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-09440-img0002.jpg "Western Blot: CPN1 Overexpression Lysate (Adult Normal) [NBL1-09440] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CPN1.")
![ELISA: Human CPN1 ELISA Kit (Colorimetric) [NBP3-27420] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-27420_human-cpn1-elisa-kit-colorimetric-11420241027322.png "ELISA: Human CPN1 ELISA Kit (Colorimetric) [NBP3-27420] - Standard Curve Reference")
![Immunohistochemistry-Paraffin: CYP11B1 Antibody [NBP2-49223] - Staining of human tonsil shows no positivity in non-germinal center cells as expected.](http://images.novusbio.com/fullsize/CYP11B1-Antibody-Immunohistochemistry-Paraffin-NBP2-49223-img0007.jpg "Immunohistochemistry-Paraffin: CYP11B1 Antibody [NBP2-49223] - Staining of human tonsil shows no positivity in non-germinal center cells as expected.")
![Immunohistochemistry-Paraffin: CYP11B1 Antibody [NBP2-49223] - Staining of human adrenal gland shows strong granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/CYP11B1-Antibody-Immunohistochemistry-Paraffin-NBP2-49223-img0004.jpg)
![Immunohistochemistry-Paraffin: CYP11B1 Antibody [NBP2-49223] - Staining of human placenta shows no positivity in trophoblastic cells as expected.](http://images.novusbio.com/fullsize/CYP11B1-Antibody-Immunohistochemistry-Paraffin-NBP2-49223-img0005.jpg)
![Immunohistochemistry-Paraffin: CYP11B1 Antibody [NBP2-49223] - Staining of human rectum shows no positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/CYP11B1-Antibody-Immunohistochemistry-Paraffin-NBP2-49223-img0006.jpg)
![Western Blot: CYP11B1 Antibody [NBP1-68883] - Titration: 0.2-1 ug/ml, Positive Control: Human Liver.](http://images.novusbio.com/fullsize/CYP11B1-Antibody-Western-Blot-NBP1-68883-img0004.jpg "Western Blot: CYP11B1 Antibody [NBP1-68883] - Titration: 0.2-1 ug/ml, Positive Control: Human Liver.")
![Immunohistochemistry: CYP11B1 Antibody [NBP1-68883] - Monkey vagina Primary Antibody Dilution: 1 : 25 Secondary Antibody: Anti-rabbit-HRP Secondary Antibody Dilution: 1 : 1000 Color/Signal Descriptions: Brown: CYP11B1 Blue: Nucleus Gene name: CYP11B1 Submitted by: Jonathan Bertin, Endoceutics Inc.](http://images.novusbio.com/fullsize/CYP11B1-Antibody-Immunohistochemistry-NBP1-68883-img0006.jpg)
![Immunohistochemistry: CYP11B1 Antibody [NBP1-68883] - Monkey adrenal gland Primary Antibody Dilution: 1 : 25 Secondary Antibody: Anti-rabbit-HRP Secondary Antibody Dilution: 1 : 1000 Color/Signal Descriptions: Brown: CYP11B1 Blue: Nucleus Gene name: CYP11B1 Submitted by: Jonathan Bertin, Endoceutics Inc.](http://images.novusbio.com/fullsize/CYP11B1-Antibody-Immunohistochemistry-NBP1-68883-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: CYP11B1 Antibody [NBP3-04876] - Analysis of U2OS cells using CYP11B1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/CYP11B1-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-04876-img0001.jpg "Immunocytochemistry/Immunofluorescence: CYP11B1 Antibody [NBP3-04876] - Analysis of U2OS cells using CYP11B1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.")
![Immunohistochemistry-Paraffin: CYP11B1 Antibody [NBP3-04876] - Immunohistochemistry of paraffin-embedded Human adrenal gland using CYP11B1 Rabbit pAb (NBP3-04876) at dilution of 1:500 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/CYP11B1 Antibody-Immunohistochemistry-Paraffin-NBP3-04876-img0002.jpg)
![Western Blot: CYP11B1 Antibody [NBP1-68882] - Hela cell lysate, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/CYP11B1-Antibody-Western-Blot-NBP1-68882-img0002.jpg "Western Blot: CYP11B1 Antibody [NBP1-68882] - Hela cell lysate, concentration 0.2-1 ug/ml.")
![Western Blot: CYP11B1 Overexpression Lysate (Adult Normal) [NBP2-04159] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CYP11B1.](http://images.novusbio.com/fullsize/CYP11B1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-04159-img0001.jpg "Western Blot: CYP11B1 Overexpression Lysate (Adult Normal) [NBP2-04159] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CYP11B1.")