Product Search for anti CD95: Novus Biologicals

NB120-13550

Fas/TNFRSF6/CD95 Antibody - BSA Free

(1 Review)

(4 Publications)

0.1 mg

NB120-13550

Immunohistochemistry-Paraffin: Fas Receptor/TNFRSF6/CD95 Antibody [NB120-13550] - Analysis of human colon tumor tissue stained with Fas polyclonal antibody, at 10ug/ml.

Immunohistochemistry-Paraffin: Fas Receptor/TNFRSF6/CD95 Antibody [NB120-13550] - Analysis of human spleen tissue stained with Fas polyclonal antibody, at 10ug/ml.

ER stress response to sustained bilirubin levels. c Representative IF of cerebellar sections at P10 using an anti-CD95/Fas antibody (green), an anti-calbindin antibody (red) to highlight Purkinje cells.

ER stress response to sustained bilirubin levels. a Relative mRNA expression analysis of different genes to assess the ER stress response over prolonged bilirubin exposure. WT and Ugt1-/- mice mRNA expression levels of ATF3, CHOP and CD95/Fas were analysed at indicated time points by qRT-PCR. For each gene, data were normalized according to the values of the WT samples at P5. For all the experiments values represent the mean +/- SD. Two-way ANOVA test, *p < 0.05. The number of WT and Ugt1-/- was ≥3 in all the experiments and time points. b Representative IF of cerebellar sections from WT and Ugt1-/- mice at P5 using an anti-CHOP antibody (red), co-stained with (left panel) an anti-calbindin antibody (green) to highlight Purkinje cells or (right panel) anti-NeuN to highlight granule cells. c Representative IF of cerebellar sections at P10 using an anti-CD95/Fas antibody (green), an anti-calbindin antibody (red) to highlight Purkinje cells. d Representative IF of cerebellar sections at P10 using an anti-CHOP antibody (red), co-stained with (left panel) an anti-calbindin antibody (green) or (right panel) anti-Iba1 to highlight microglia. e Representative IF of cerebellar sections at P10 using an anti-Calbindin antibody (red), co-stained with an anti-Iba1 antibody (green). For IF, Hoechst dye (blue) was used to mark nuclei. Scale bar 50 μm Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/28340583), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB120-13550AF350
Alexa Fluor 405	NB120-13550AF405
Alexa Fluor 488	NB120-13550AF488
Alexa Fluor 532	NB120-13550AF532
Alexa Fluor 594	NB120-13550AF594
Alexa Fluor 647	NB120-13550AF647
Alexa Fluor 700	NB120-13550AF700
Alexa Fluor 750	NB120-13550AF750
Alexa Fluor Plus 405	NB120-13550AFP405
Alexa Fluor Plus 488	NB120-13550AFP488
Alexa Fluor Plus 555	NB120-13550AFP555
Alexa Fluor Plus 594	NB120-13550AFP594
Alexa Fluor Plus 647	NB120-13550AFP647
Alexa Fluor Plus 680	NB120-13550AFP680
Allophycocyanin	NB120-13550APC
Biotin	NB120-13550B
CoraFluor 1	NB120-13550CL1
DyLight 350	NB120-13550UV
DyLight 405	NB120-13550V
DyLight 488	NB120-13550G
DyLight 550	NB120-13550R
DyLight 594	NB120-13550DL594
DyLight 650	NB120-13550C
DyLight 680	NB120-13550FR
DyLight 755	NB120-13550IR
FITC	NB120-13550F
HRP	NB120-13550H
Janelia Fluor 525	NB120-13550JF525
Janelia Fluor 549	NB120-13550JF549
Janelia Fluor 646	NB120-13550JF646
Janelia Fluor 669	NB120-13550JF669
PE	NB120-13550PE
PerCP	NB120-13550PCP
mFluor Violet 450 SE	NB120-13550MFV450
mFluor Violet 500 SE	NB120-13550MFV500
mFluor Violet 610 SE	NB120-13550MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB120-13550-0.025mg

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AF326

Fas/TNFRSF6/CD95 Antibody [Unconjugated]

(8 Publications)

100 μg

AF326

HAF019). A specific band was detected for Fas/TNFRSF6/CD95 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Fas/TNFRSF6/CD95 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF326) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

HAF019). A specific band was detected for Fas/TNFRSF6/CD95 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Fas/TNFRSF6/CD95 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF326) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Fas/TNFRSF6/CD95 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

326-FS) was serially diluted and captured by Mouse Anti-Human Fas/TNFRSF6/CD95 Monoclonal Antibody (Catalog # MAB144) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human Fas/TNFRSF6/CD95 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF326) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox">

Recombinant Human Fas/TNFRSF6/CD95 Fc Chimera (Catalog # <a class=

326-FS) was serially diluted and captured by Mouse Anti-Human Fas/TNFRSF6/CD95 Monoclonal Antibody (Catalog # MAB144) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human Fas/TNFRSF6/CD95 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF326) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF326U-100UG
Alexa Fluor 405	AF326V-100UG
Alexa Fluor 488	AF326G-100UG
Alexa Fluor 532	AF326X-100UG
Alexa Fluor 594	AF326T-100UG
Alexa Fluor 647	AF326R-100UG
Alexa Fluor 700	AF326N-100UG
Alexa Fluor 750	AF326S-100UG
Alexa Fluor Plus 405	AF326AFP405-100UG
Alexa Fluor Plus 488	AF326AFP488-100UG
Alexa Fluor Plus 555	AF326AFP555-100UG
Alexa Fluor Plus 594	AF326AFP594-100UG
Alexa Fluor Plus 647	AF326AFP647-100UG
Alexa Fluor Plus 680	AF326AFP680-100UG
Allophycocyanin	AF326A
Biotin	BAF326
CoraFluor 1	AF326CL1
DyLight 350	AF326D
DyLight 405	AF326E
DyLight 488	AF326K
DyLight 550	AF326L
DyLight 594	AF326M
DyLight 650	AF326W
DyLight 680	AF326Y
DyLight 755	AF326Z
FITC	AF326F
HRP	AF326H
Janelia Fluor 525	AF326JF525
Janelia Fluor 549	AF326I
Janelia Fluor 646	AF326J
Janelia Fluor 669	AF326JF669
PE	AF326P
PerCP	AF326C
mFluor Violet 450 SE	AF326MFV450
mFluor Violet 500 SE	AF326MFV500
mFluor Violet 610 SE	AF326MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF326-SP

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MAB142

Fas/TNFRSF6/CD95 Antibody (DX2) [Unconjugated]

(6 Publications)

500 μg

MAB142

Modulation of Fas cell surface expression in cells infected with HCMV. (a) HFFF-hTERTs were infected with HCMV strain Merlin (m.o.i. 10) or mock-infected, and analysed at indicated time points by flow cytometry for cell surface Fas expression [mAb142 (R&D Systems), n≥3). (b) HFFFs were infected with HCMV strain Merlin (m.o.i. 10, 72 h) in the presence (i) or absence (ii) of 100 µM ganciclovir and analysed by flow cytometry for cell surface Fas expression (n = 3). (c) HFFF-hTERTs were incubated with supernatants (sn) of strain Merlin-infected cells (m.o.i. 10, 72 h p.i.) from which virions had been removed using a 0.1 µm filter, or were infected with HCMV strain Merlin (m.o.i. 10, 72 h) or an equivalent gamma -irradiated preparation (2500 Gy) and analysed by flow cytometry for cell surface Fas expression (n = 3). (d) HFFF-hTERTs were infected with HCMV strains Merlin, AD169, FIX or TB40 (m.o.i. 10, 72 h) and analysed by flow cytometry for cell surface Fas expression (n≥3). Control IgG staining is denoted by black and grey lines for mock-infected and HCMV-infected cells, respectively. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/24394698), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB142U-100UG
Alexa Fluor 405	FAB142V-100UG
Alexa Fluor 488	FAB142G-100UG
Alexa Fluor 532	FAB142X-100UG
Alexa Fluor 594	FAB142T-100UG
Alexa Fluor 647	FAB142R-100UG
Alexa Fluor 700	FAB142N-100UG
Alexa Fluor 750	FAB142S-100UG
Alexa Fluor Plus 405	FAB142AFP405-100UG
Alexa Fluor Plus 488	FAB142AFP488-100UG
Alexa Fluor Plus 555	FAB142AFP555-100UG
Alexa Fluor Plus 594	FAB142AFP594-100UG
Alexa Fluor Plus 647	FAB142AFP647-100UG
Alexa Fluor Plus 680	FAB142AFP680-100UG
Biotin	FAB142B
CoraFluor 1	FAB142CL1
DyLight 350	FAB142D
DyLight 405	FAB142E
DyLight 488	FAB142K
DyLight 550	FAB142L
DyLight 594	FAB142M
DyLight 650	FAB142W
DyLight 680	FAB142Y
DyLight 755	FAB142Z
HRP	FAB142H
Janelia Fluor 525	FAB142JF525
Janelia Fluor 549	FAB142I
Janelia Fluor 646	FAB142J
Janelia Fluor 669	FAB142JF669
mFluor Violet 450 SE	FAB142MFV450
mFluor Violet 500 SE	FAB142MFV500
mFluor Violet 610 SE	FAB142MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB142-SP

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AF435

Fas/TNFRSF6/CD95 Antibody [Unconjugated]

(3 Publications)

100 μg

AF435

CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to olfactory epithelial cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_lightbox">

Fas/TNFRSF6/CD95 was detected in immersion fixed frozen sections of mouse embryo (E15) using Goat Anti-Mouse Fas/TNFRSF6/CD95 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF435) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=

CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to olfactory epithelial cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." alt="Fas/TNFRSF6/CD95 was detected in immersion fixed frozen sections of mouse embryo (E15) using Goat Anti-Mouse Fas/TNFRSF6/CD95 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF435) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to olfactory epithelial cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_thumb" />

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

, , , ,

Applications: , , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF435U-100UG
Alexa Fluor 405	AF435V-100UG
Alexa Fluor 488	AF435G-100UG
Alexa Fluor 532	AF435X-100UG
Alexa Fluor 594	AF435T-100UG
Alexa Fluor 647	AF435R-100UG
Alexa Fluor 700	AF435N-100UG
Alexa Fluor 750	AF435S-100UG
Alexa Fluor Plus 405	AF435AFP405-100UG
Alexa Fluor Plus 488	AF435AFP488-100UG
Alexa Fluor Plus 555	AF435AFP555-100UG
Alexa Fluor Plus 594	AF435AFP594-100UG
Alexa Fluor Plus 647	AF435AFP647-100UG
Alexa Fluor Plus 680	AF435AFP680-100UG
Biotin	BAF435
CoraFluor 1	AF435CL1
DyLight 350	AF435D
DyLight 405	AF435E
DyLight 488	AF435K
DyLight 550	AF435L
DyLight 594	AF435M
DyLight 650	AF435W
DyLight 680	AF435Y
DyLight 755	AF435Z
FITC	AF435F
HRP	AF435H
Janelia Fluor 525	AF435JF525
Janelia Fluor 549	AF435I
Janelia Fluor 646	AF435J
Janelia Fluor 669	AF435JF669
PE	AF435P
PerCP	AF435C
mFluor Violet 450 SE	AF435MFV450
mFluor Violet 500 SE	AF435MFV500
mFluor Violet 610 SE	AF435MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF435-SP

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AF2159

Fas/TNFRSF6/CD95 Antibody [Unconjugated]

(2 Publications)

100 μg

AF2159

CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to hepatocyte cell membranes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_lightbox">

Fas/TNFRSF6/CD95 was detected in perfusion fixed frozen sections of rat liver using Goat Anti-Rat Fas/TNFRSF6/CD95 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2159) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=

CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to hepatocyte cell membranes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." alt="Fas/TNFRSF6/CD95 was detected in perfusion fixed frozen sections of rat liver using Goat Anti-Rat Fas/TNFRSF6/CD95 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2159) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to hepatocyte cell membranes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_thumb" />

Reactivity:

Rt

Species Glossary

Reactivity: Rt

Applications:

, , ,

Applications: , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF2159U-100UG
Alexa Fluor 405	AF2159V-100UG
Alexa Fluor 488	AF2159G-100UG
Alexa Fluor 532	AF2159X-100UG
Alexa Fluor 594	AF2159T-100UG
Alexa Fluor 647	AF2159R-100UG
Alexa Fluor 700	AF2159N-100UG
Alexa Fluor 750	AF2159S-100UG
Alexa Fluor Plus 405	AF2159AFP405-100UG
Alexa Fluor Plus 488	AF2159AFP488-100UG
Alexa Fluor Plus 555	AF2159AFP555-100UG
Alexa Fluor Plus 594	AF2159AFP594-100UG
Alexa Fluor Plus 647	AF2159AFP647-100UG
Alexa Fluor Plus 680	AF2159AFP680-100UG
Allophycocyanin	AF2159A
Biotin	BAF2159
CoraFluor 1	AF2159CL1
DyLight 350	AF2159D
DyLight 405	AF2159E
DyLight 488	AF2159K
DyLight 550	AF2159L
DyLight 594	AF2159M
DyLight 650	AF2159W
DyLight 680	AF2159Y
DyLight 755	AF2159Z
FITC	AF2159F
HRP	AF2159H
Janelia Fluor 525	AF2159JF525
Janelia Fluor 549	AF2159I
Janelia Fluor 646	AF2159J
Janelia Fluor 669	AF2159JF669
PE	AF2159P
PerCP	AF2159C
mFluor Violet 450 SE	AF2159MFV450
mFluor Violet 500 SE	AF2159MFV500
mFluor Violet 610 SE	AF2159MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF2159-SP

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AF2267

Fas/TNFRSF6/CD95 Antibody

(2 Publications)

100 μg

AF2267

AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107)." class="big_lightbox">

Feline peripheral blood mononuclear cells were stained with Feline Fas/TNFRSF6/CD95 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2267, filled histogram) or isotype control antibody (Catalog # <a class=

AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107)." alt="Feline peripheral blood mononuclear cells were stained with Feline Fas/TNFRSF6/CD95 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2267, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107)." class="big_thumb" />

NL001) and counterstained with DAPI (blue). Specific staining was localized to the plasma membrane. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox">

Fas/TNFRSF6/CD95 was detected in immersion fixed feline peripheral blood mononuclear cells (PBMCs) using Goat Anti-Feline Fas/TNFRSF6/CD95 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2267) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # <a class=

NL001) and counterstained with DAPI (blue). Specific staining was localized to the plasma membrane. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

Reactivity:

Fe

Species Glossary

Reactivity: Fe

Applications:

, , ,

Applications: , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF2267U-100UG
Alexa Fluor 405	AF2267V-100UG
Alexa Fluor 488	AF2267G-100UG
Alexa Fluor 532	AF2267X-100UG
Alexa Fluor 594	AF2267T-100UG
Alexa Fluor 647	AF2267R-100UG
Alexa Fluor 700	AF2267N-100UG
Alexa Fluor 750	AF2267S-100UG
Alexa Fluor Plus 405	AF2267AFP405-100UG
Alexa Fluor Plus 488	AF2267AFP488-100UG
Alexa Fluor Plus 555	AF2267AFP555-100UG
Alexa Fluor Plus 594	AF2267AFP594-100UG
Alexa Fluor Plus 647	AF2267AFP647-100UG
Alexa Fluor Plus 680	AF2267AFP680-100UG
Allophycocyanin	AF2267A
Biotin	BAF2267
CoraFluor 1	AF2267CL1
DyLight 350	AF2267D
DyLight 405	AF2267E
DyLight 488	AF2267K
DyLight 550	AF2267L
DyLight 594	AF2267M
DyLight 650	AF2267W
DyLight 680	AF2267Y
DyLight 755	AF2267Z
FITC	AF2267F
HRP	AF2267H
Janelia Fluor 525	AF2267JF525
Janelia Fluor 549	AF2267I
Janelia Fluor 646	AF2267J
Janelia Fluor 669	AF2267JF669
PE	AF2267P
PerCP	AF2267C
mFluor Violet 450 SE	AF2267MFV450
mFluor Violet 500 SE	AF2267MFV500
mFluor Violet 610 SE	AF2267MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF2267-SP

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NBP2-37515

Fas/TNFRSF6/CD95 Antibody (4F8H6) - BSA Free

0.1 ml

NBP2-37515

Flow Cytometry: Fas Receptor/TNFRSF6/CD95 Antibody (4F8H6) [NBP2-37515] - An intracellular stain was performed on HeLa cells with Fas/TNFRSF6/CD95 Antibody (4F8H6) NBP2-37515R (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Dylight 550.

Western Blot: Fas/TNFRSF6/CD95 Antibody (4F8H6) - BSA Free [NBP2-37515] - Analysis using Fas/TNFRSF6/CD95 monoclonal antibody against human Fas/TNFRSF6/CD95 (AA: 87-278) recombinant protein. (Expected MW is 47.2 kDa)

Flow Cytometry: Fas/TNFRSF6/CD95 Antibody (4F8H6) - BSA Free [NBP2-37515] - Analysis of Hela cells using Fas/TNFRSF6/CD95 mouse monoclonal antibody (green) and negative control (red).

ELISA: Fas Receptor/TNFRSF6/CD95 Antibody (4F8H6) [NBP2-37515] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , , ,

Applications: , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-37515-0.025ml

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NBP1-89034

Fas/TNFRSF6/CD95 Antibody - BSA Free

(1 Publication)

0.1 ml

NBP1-89034

<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Fas/TNFRSF6/CD95 Antibody [NBP1-89034] - Staining in human rectum and skeletal muscle tissues using anti-Fas/TNFRSF6/CD95 antibody. Corresponding Fas/TNFRSF6/CD95 RNA-seq data are presented for the same tissues.

Immunohistochemistry-Paraffin: Fas Receptor/TNFRSF6/CD95 Antibody [NBP1-89034] - Fas/TNFRSF6/CD95 Antibody [NBP1-89034] - Staining of human rectum shows high expression.

Immunohistochemistry-Paraffin: Fas Receptor/TNFRSF6/CD95 Antibody [NBP1-89034] - Fas/TNFRSF6/CD95 Antibody [NBP1-89034] - Staining of human skeletal muscle shows low expression as expected.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-89034-25ul

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MAB144

Fas/TNFRSF6/CD95 Antibody (50830)

(2 Publications)

500 μg

MAB144

326-FS) was serially diluted and captured by Mouse Anti-Human Fas/TNFRSF6/CD95 Monoclonal Antibody (Catalog # MAB144) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human Fas/TNFRSF6/CD95 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF326) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox">

326-FS) was serially diluted and captured by Mouse Anti-Human Fas/TNFRSF6/CD95 Monoclonal Antibody (Catalog # MAB144) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human Fas/TNFRSF6/CD95 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF326) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" alt="Recombinant Human Fas/TNFRSF6/CD95 Fc Chimera (Catalog # 326-FS) was serially diluted and captured by Mouse Anti-Human Fas/TNFRSF6/CD95 Monoclonal Antibody (Catalog # MAB144) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human Fas/TNFRSF6/CD95 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF326) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB144U-100UG
Alexa Fluor 405	FAB144V-100UG
Alexa Fluor 488	FAB144G-100UG
Alexa Fluor 532	FAB144X-100UG
Alexa Fluor 594	FAB144T-100UG
Alexa Fluor 647	FAB144R-100UG
Alexa Fluor 700	FAB144N-100UG
Alexa Fluor 750	FAB144S-100UG
Alexa Fluor Plus 405	FAB144AFP405-100UG
Alexa Fluor Plus 488	FAB144AFP488-100UG
Alexa Fluor Plus 555	FAB144AFP555-100UG
Alexa Fluor Plus 594	FAB144AFP594-100UG
Alexa Fluor Plus 647	FAB144AFP647-100UG
Alexa Fluor Plus 680	FAB144AFP680-100UG
Allophycocyanin	FAB144A
Biotin	FAB144B
CoraFluor 1	FAB144CL1
DyLight 350	FAB144D
DyLight 405	FAB144E
DyLight 488	FAB144K
DyLight 550	FAB144L
DyLight 594	FAB144M
DyLight 650	FAB144W
DyLight 680	FAB144Y
DyLight 755	FAB144Z
FITC	FAB144F
HRP	FAB144H
Janelia Fluor 525	FAB144JF525
Janelia Fluor 549	FAB144I
Janelia Fluor 646	FAB144J
Janelia Fluor 669	FAB144JF669
PE	FAB144P
PerCP	FAB144C
mFluor Violet 450 SE	FAB144MFV450
mFluor Violet 500 SE	FAB144MFV500
mFluor Violet 610 SE	FAB144MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB144-SP

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MAB2267

Fas/TNFRSF6/CD95 Antibody (431014) [Unconjugated]

(1 Publication)

100 μg

MAB2267

MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0102B)." class="big_lightbox">

<b>Biological Strategies Validation. </b>Feline peripheral blood mononuclear cells were treated with PMA and Ca<SUP>2+</SUP>ionomycin, then stained with Mouse Anti-Feline Fas/ TNFRSF6/CD95 Mono-clonal Antibody (Catalog # MAB2267, filled histogram) or isotype control antibody (Catalog # <a class=

MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0102B)." alt="Feline peripheral blood mononuclear cells were treated with PMA and Ca²⁺ionomycin, then stained with Mouse Anti-Feline Fas/ TNFRSF6/CD95 Mono-clonal Antibody (Catalog # MAB2267, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0102B)." class="big_thumb" />

Reactivity:

Fe

Species Glossary

Reactivity: Fe

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB2267U-100UG
Alexa Fluor 405	FAB2267V-100UG
Alexa Fluor 488	FAB2267G-100UG
Alexa Fluor 532	FAB2267X
Alexa Fluor 594	FAB2267T-100UG
Alexa Fluor 647	FAB2267R-100UG
Alexa Fluor 700	FAB2267N-100UG
Alexa Fluor 750	FAB2267S-100UG
Alexa Fluor Plus 405	FAB2267AFP405-100UG
Alexa Fluor Plus 488	FAB2267AFP488-100UG
Alexa Fluor Plus 555	FAB2267AFP555-100UG
Alexa Fluor Plus 594	FAB2267AFP594-100UG
Alexa Fluor Plus 647	FAB2267AFP647-100UG
Alexa Fluor Plus 680	FAB2267AFP680-100UG
Allophycocyanin	FAB2267A
Allophycocyanin/Cy7	FAB2267APCCY7
Biotin	FAB2267B
CoraFluor 1	FAB2267CL1
DyLight 350	FAB2267D
DyLight 405	FAB2267E
DyLight 488	FAB2267K
DyLight 550	FAB2267L
DyLight 594	FAB2267M
DyLight 650	FAB2267W
DyLight 680	FAB2267Y
DyLight 755	FAB2267Z
FITC	FAB2267F
HRP	FAB2267H
Janelia Fluor 525	FAB2267JF525
Janelia Fluor 549	FAB2267I
Janelia Fluor 646	FAB2267J
Janelia Fluor 669	FAB2267JF669
PE	FAB2267P
PE/Atto594	FAB2267PEATT594
PE/Cy5.5	FAB2267PECY55
PE/Cy7	FAB2267PECY7
PerCP	FAB2267C
mFluor Violet 450 SE	FAB2267MFV450
mFluor Violet 500 SE	FAB2267MFV500
mFluor Violet 610 SE	FAB2267MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB2267-SP
Carrier Free	FAB2267V-100UG
Carrier Free	FAB2267U-100UG
Carrier Free	FAB2267T-100UG
Carrier Free	FAB2267S-100UG
Carrier Free	FAB2267R-100UG
Carrier Free	FAB2267N-100UG
Carrier Free	FAB2267G-100UG

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NB500-503

Fas/TNFRSF6/CD95 Antibody (LT95) - BSA Free

(2 Publications)

0.1 mg

NB500-503

Flow Cytometry: Fas Receptor/TNFRSF6/CD95 Antibody (LT95) [NB500-503] - Fas/TNFRSF6/CD95 Antibody (LT95) [NB500-503] - A surface stain was performed on HeLa with Fas/TNFRSF6/CD95 Antibody (LT95) NB500-503AF647 and a matched isotype control. Cells were incubated in an antibody dilution of 2.5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

Flow Cytometry: Fas Receptor/TNFRSF6/CD95 Antibody (LT95) [NB500-503] - Fas/TNFRSF6/CD95 Antibody (LT95) [NB500-503] - Using the Allophycocyanin direct conjugate Surface staining of human peripheral blood cells with anti-CD95 (LT95) APC.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB500-503AF350
Alexa Fluor 405	NB500-503AF405
Alexa Fluor 488	NB500-503AF488
Alexa Fluor 532	NB500-503AF532
Alexa Fluor 594	NB500-503AF594
Alexa Fluor 647	NB500-503AF647
Alexa Fluor 700	NB500-503AF700
Alexa Fluor 750	NB500-503AF750
Alexa Fluor Plus 405	NB500-503AFP405
Alexa Fluor Plus 488	NB500-503AFP488
Alexa Fluor Plus 555	NB500-503AFP555
Alexa Fluor Plus 594	NB500-503AFP594
Alexa Fluor Plus 647	NB500-503AFP647
Alexa Fluor Plus 680	NB500-503AFP680
Allophycocyanin	NB500-503APC
Allophycocyanin/Cy7	NB500-503APCCY7
Biotin	NB500-503B
CoraFluor 1	NB500-503CL1
DyLight 350	NB500-503UV
DyLight 405	NB500-503V
DyLight 488	NB500-503G
DyLight 550	NB500-503R
DyLight 594	NB500-503DL594
DyLight 650	NB500-503C
DyLight 680	NB500-503FR
DyLight 755	NB500-503IR
FITC	NB500-503F
HRP	NB500-503H
Janelia Fluor 525	NB500-503JF525
Janelia Fluor 549	NB500-503JF549
Janelia Fluor 646	NB500-503JF646
Janelia Fluor 669	NB500-503JF669
PE	NB500-503PE
PE/Atto594	NB500-503PEATT594
PE/Cy5.5	NB500-503PECY55
PE/Cy7	NB500-503PECY7
PerCP	NB500-503PCP
mFluor Violet 450 SE	NB500-503MFV450
mFluor Violet 500 SE	NB500-503MFV500
mFluor Violet 610 SE	NB500-503MFV610

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NBP2-13078

Fas/TNFRSF6/CD95 Antibody (4F8D6) - BSA Free

(2 Publications)

0.1 mg

NBP2-13078

Western Blot: Fas/TNFRSF6/CD95 Antibody (4F8D6) - BSA Free [NBP2-13078] - Figure 2: Western blot analysis using Fas/TNFRSF6/CD95 monoclonal antibody against human Fas/TNFRSF6/CD95 (AA: 87-278) recombinant protein. (Expected MW is 47.2 kDa)

ELISA: Fas Receptor/TNFRSF6/CD95 Antibody (4F8D6) [NBP2-13078] - Fas/TNFRSF6/CD95 Antibody (4F8D6) [NBP2-13078] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-13078-0.025mg

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Antibody Validation

Reviews

Host

Available Conjugates

Format

Modification

Clonality

Clone

Gene Symbol

Images Type

Isotype

Preservative

Research Areas

Has Sample Size

Promotional Item

Species Glossary

Fas/TNFRSF6/CD95 Antibody - BSA Free

Fas/TNFRSF6/CD95 Antibody [Unconjugated]

Fas/TNFRSF6/CD95 Antibody (DX2) [Unconjugated]

Fas/TNFRSF6/CD95 Antibody [Unconjugated]

Fas/TNFRSF6/CD95 Antibody [Unconjugated]

Fas/TNFRSF6/CD95 Antibody

Fas/TNFRSF6/CD95 Antibody (4F8H6) - BSA Free

Fas/TNFRSF6/CD95 Antibody - BSA Free

Fas/TNFRSF6/CD95 Antibody (50830)

Fas/TNFRSF6/CD95 Antibody (431014) [Unconjugated]

Fas/TNFRSF6/CD95 Antibody (LT95) - BSA Free

Fas/TNFRSF6/CD95 Antibody (4F8D6) - BSA Free