| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59310-25ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-33806-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-05549-50ul | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| FL490 | NBP3-52379 | |||
| FL550 | NBP3-52380 | |||
| FL594 | NBP3-52381 | |||
| FL650 | NBP3-52382 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-52340-25ul | |||
| Azide and BSA Free | NBP3-52341 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-05528-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59315-25ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59374-25ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-05550-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-34023-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-58275-25ul | |||
![Western Blot: Ankyrin Brain Antibody [NBP2-33863] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue](http://images.novusbio.com/fullsize/Ankyrin-Brain-Antibody-Western-Blot-NBP2-33863-img0003.jpg "Western Blot: Ankyrin Brain Antibody [NBP2-33863] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue")
![Immunohistochemistry-Paraffin: Ankyrin Brain Antibody [NBP2-33863] - Staining of human placenta shows no positivity in trophoblastic cells as expected.](http://images.novusbio.com/fullsize/Ankyrin-Brain-Antibody-Immunohistochemistry-Paraffin-NBP2-33863-img0012.jpg)
![Immunohistochemistry-Paraffin: Ankyrin Brain Antibody [NBP2-33863] - Staining of human cerebral cortex shows high expression.](http://images.novusbio.com/fullsize/Ankyrin-Brain-Antibody-Immunohistochemistry-Paraffin-NBP2-33863-img0004.jpg)
![Immunohistochemistry-Paraffin: Ankyrin Brain Antibody [NBP2-33863] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/Ankyrin-Brain-Antibody-Immunohistochemistry-Paraffin-NBP2-33863-img0005.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Ankyrin Brain Antibody [NBP2-33863] - Analysis in human cerebral cortex and skin tissues using NBP2-33863 antibody. Corresponding ANK2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Ankyrin-Brain-Antibody-Immunohistochemistry-Paraffin-NBP2-33863-img0009.jpg)
![Immunohistochemistry-Paraffin: Ankyrin Brain Antibody [NBP2-33863] - Staining of human cerebral cortex shows strong positivity in neuropil.](http://images.novusbio.com/fullsize/Ankyrin-Brain-Antibody-Immunohistochemistry-Paraffin-NBP2-33863-img0010.jpg)
![Immunohistochemistry-Paraffin: Ankyrin Brain Antibody [NBP2-33863] - Staining of human kidney shows weak to moderate cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/Ankyrin-Brain-Antibody-Immunohistochemistry-Paraffin-NBP2-33863-img0011.jpg)
![Immunohistochemistry-Paraffin: Ankyrin Brain Antibody [NBP2-33863] - Staining of human skin shows no positivity in squamous epithelial cells as expected.](http://images.novusbio.com/fullsize/Ankyrin-Brain-Antibody-Immunohistochemistry-Paraffin-NBP2-33863-img0013.jpg)
![Western Blot: Ankyrin 3 Antibody (S106-20) [NBP2-59310] - Western Blot analysis of Rat brain membrane lysate showing detection of Ankyrin 3 protein using Mouse Anti-Ankyrin 3 Monoclonal Antibody, Clone S106-20 (NBP2-59310). Primary Antibody: Mouse Anti-Ankyrin 3 Monoclonal Antibody (NBP2-59310) at 1:1000.](http://images.novusbio.com/fullsize/Ankyrin-3-Antibody-S106-20-Western-Blot-NBP2-59310-img0005.jpg "Western Blot: Ankyrin 3 Antibody (S106-20) [NBP2-59310] - Western Blot analysis of Rat brain membrane lysate showing detection of Ankyrin 3 protein using Mouse Anti-Ankyrin 3 Monoclonal Antibody, Clone S106-20 (NBP2-59310). Primary Antibody: Mouse Anti-Ankyrin 3 Monoclonal Antibody (NBP2-59310) at 1:1000.")
![Immunocytochemistry/Immunofluorescence: Ankyrin 3 Antibody (S106-20) [NBP2-59310] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Ankyrin 3 Monoclonal Antibody, Clone S106-20 (NBP2-59310). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Ankyrin 3 Monoclonal Antibody (NBP2-59310) at 1:100 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Ankyrin 3 Antibody (D) Composite.](http://images.novusbio.com/fullsize/Ankyrin-3-Antibody-S106-20-Immunocytochemistry-Immunofluorescence-NBP2-59310-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Ankyrin 3 Antibody (S106-20) [NBP2-59310] - Tissue: SK-N-BE Cells (Human Neuroblastoma cells). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Ankyrin G Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm . Magnification: 60X.](http://images.novusbio.com/fullsize/Ankyrin-3-Antibody-S106-20-Immunocytochemistry-Immunofluorescence-NBP2-59310-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Ankyrin 3 Antibody (S106-20) [NBP2-59310] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Ankyrin 3 Monoclonal Antibody, Clone S106-20 (NBP2-59310). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Ankyrin 3 Monoclonal Antibody (NBP2-59310) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm . Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Ankyrin 3 Antibody. (D) Composite.](http://images.novusbio.com/fullsize/Ankyrin-3-Antibody-S106-20-Immunocytochemistry-Immunofluorescence-NBP2-59310-img0007.jpg)
![Western Blot: Ankyrin 1 Antibody [NBP1-71805] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Affinity purified rabbit anti-Ankyrin 1 antibody used for WB at 0.4 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. Ankyrin 1 was also immunoprecipitated by another rabbit anti-Ankyrin 1 antibody, which recognizes an upstream epitope.](http://images.novusbio.com/fullsize/Ankyrin-1-Antibody-Western-Blot-NBP1-71805-img0002.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: Ankyrin 1 Antibody [NBP1-71805] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Affinity purified rabbit anti-Ankyrin 1 antibody used for WB at 0.4 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. Ankyrin 1 was also immunoprecipitated by another rabbit anti-Ankyrin 1 antibody, which recognizes an upstream epitope.")
![Western Blot: Ankyrin 1 Antibody [NBP2-33806] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/Ankyrin-1-Antibody-Western-Blot-NBP2-33806-img0005.jpg "Western Blot: Ankyrin 1 Antibody [NBP2-33806] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp")
![Immunohistochemistry-Paraffin: Ankyrin 1 Antibody [NBP2-33806] - Staining of human bone marrow shows strong cytoplasmic positivity in hematopoietic cells.](http://images.novusbio.com/fullsize/Ankyrin-1-Antibody-Immunohistochemistry-Paraffin-NBP2-33806-img0001.jpg)
![Immunohistochemistry-Paraffin: Ankyrin 1 Antibody [NBP2-33806] - Staining of human cerebellum shows weak membranous and cytoplasmic positivity in Purkinje cells.](http://images.novusbio.com/fullsize/antibody/nbp2-33806_rabbit-polyclonal-ankyrin-1-antibody-immunohistochemistry-paraffin-244202475354..jpg)
![Immunohistochemistry-Paraffin: Ankyrin 1 Antibody [NBP2-33806] - Staining of human skin shows no positivity in squamous epithelial cells as expected.](http://images.novusbio.com/fullsize/antibody/nbp2-33806_rabbit-polyclonal-ankyrin-1-antibody-immunohistochemistry-paraffin-244202475456..jpg)
![Immunohistochemistry-Paraffin: Ankyrin 1 Antibody [NBP2-33806] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/antibody/nbp2-33806_rabbit-polyclonal-ankyrin-1-antibody-immunohistochemistry-paraffin-244202475550..jpg)
![Western Blot: Ankyrin 3 Antibody [NBP3-05549] - Western blot analysis of different tissue and cell lysates using Ankyrin 3 Antibody at a dilution of 1:3,000, in green. [1, 6] Protein standard, [2] Rat brain, [3] Cow cortex, [4] HEK293 cells, [5] COS-1 cells, [7] Rat cortex, [8] rat cortex membrane enriched fraction, [9] mouse cortex, [10] mouse cortex membrane enriched fraction. Bands at 60-190kDa represent Ankyrin 3 splice variants and proteolytic fragments. Larger Ankyrin 3 isoforms of 270kDa and 480kDa can be seen on longer exposure of the blots.](http://images.novusbio.com/fullsize/Ankyrin-3-Antibody-Western-Blot-NBP3-05549-img0002.jpg "Western Blot: Ankyrin 3 Antibody [NBP3-05549] - Western blot analysis of different tissue and cell lysates using Ankyrin 3 Antibody at a dilution of 1:3,000, in green. [1, 6] Protein standard, [2] Rat brain, [3] Cow cortex, [4] HEK293 cells, [5] COS-1 cells, [7] Rat cortex, [8] rat cortex membrane enriched fraction, [9] mouse cortex, [10] mouse cortex membrane enriched fraction. Bands at 60-190kDa represent Ankyrin 3 splice variants and proteolytic fragments. Larger Ankyrin 3 isoforms of 270kDa and 480kDa can be seen on longer exposure of the blots.")
![Immunocytochemistry/Immunofluorescence: Ankyrin 3 Antibody [NBP3-05549] - Immunofluorescent analysis of cortical neuron-glial cell culture from E20 rat stained with Ankyrin 3 Antibody, CPCA-ANK3, at a dilution of 1:2,000 in green, and costained with mouse mAb to RBFOX3/NeuN at a dilution of 1:2,000 in red. The blue is Hoechst staining of nuclear DNA. The Ankyrin 3 antibody stains the axonal initial segments, while the RBFOX3/NeuN antibody reveals perikarya and nuclei of neurons.](http://images.novusbio.com/fullsize/Ankyrin-3-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05549-img0001.jpg)
![Western Blot: Ankyrin 3 Antibody [NBP3-05528] - Western blot analysis of different tissue and cell lysates using Ankyrin 3 Antibody, at a at a dilution of of 1:2,000 in green: [1] protein standard (red), [2] NIH-3T3, [3] C6, [4] HEK293, [5] HeLa, [6] SH-SY5Y cells, [7] rat brain, and [8] mouse brain lysates. Bands at ~190kDa represent ankyrin 3 splice variants, higher molecular weight bands at 270kDa and 480kDa can be seen on longer exposure. Lower molecular weight bands are likely proteolytic fragments.](http://images.novusbio.com/fullsize/Ankyrin-3-Antibody-Western-Blot-NBP3-05528-img0002.jpg "Western Blot: Ankyrin 3 Antibody [NBP3-05528] - Western blot analysis of different tissue and cell lysates using Ankyrin 3 Antibody, at a at a dilution of of 1:2,000 in green: [1] protein standard (red), [2] NIH-3T3, [3] C6, [4] HEK293, [5] HeLa, [6] SH-SY5Y cells, [7] rat brain, and [8] mouse brain lysates. Bands at ~190kDa represent ankyrin 3 splice variants, higher molecular weight bands at 270kDa and 480kDa can be seen on longer exposure. Lower molecular weight bands are likely proteolytic fragments.")
![Immunocytochemistry/Immunofluorescence: Ankyrin 3 Antibody [NBP3-05528] - Immunofluorescent analysis of cortex section of rat brain stained with Ankyrin 3 Antibody at a at a dilution of of 1:2,000 in green and costained with rabbit polyclonal antibody to NF-L at a at a dilution of of 1:5,000 in red. The blue is Hoechst staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. The Ankyrin 3 Antibody stains the axonal initial segments, while the NF-L antibody labels dendrites and axons of neuronal cells.](http://images.novusbio.com/fullsize/Ankyrin-3-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05528-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Ankyrin 3 Antibody [NBP3-05528] - Immunofluorescent analysis of cortex section of rat brain stained with Ankyrin 3 Antibody at a at a dilution of of 1:2,000 in green and costained with rabbit polyclonal antibody to NF-L at a at a dilution of of 1:5,000 in red. The blue is Hoechst staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. The Ankyrin 3 Antibody stains the axonal initial segments, while the NF-L antibody labels dendrites and axons of neuronal cells.](http://images.novusbio.com/fullsize/Ankyrin-3-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05528-img0001.jpg)
![Western Blot: Ankyrin 1 Antibody (S388A-10) [NBP2-59315] - Western Blot analysis of Rat Brain showing detection of ~200 kDa Ankyrin-R protein using Mouse Anti-Ankyrin-R Monoclonal Antibody, Clone S388A-10 (NBP2-59315). Lane 1: MW Ladder. Lane 2: Rat Brain. Load: 20 ug. Block: 2% GE Healthcare Blocker for 1 hour at RT. Primary Antibody: Mouse Anti-Ankyrin-R Monoclonal Antibody (NBP2-59315) at 1:1000 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~200 kDa.](http://images.novusbio.com/fullsize/Ankyrin-1-Antibody-S388A-10-Western-Blot-NBP2-59315-img0005.jpg "Western Blot: Ankyrin 1 Antibody (S388A-10) [NBP2-59315] - Western Blot analysis of Rat Brain showing detection of ~200 kDa Ankyrin-R protein using Mouse Anti-Ankyrin-R Monoclonal Antibody, Clone S388A-10 (NBP2-59315). Lane 1: MW Ladder. Lane 2: Rat Brain. Load: 20 ug. Block: 2% GE Healthcare Blocker for 1 hour at RT. Primary Antibody: Mouse Anti-Ankyrin-R Monoclonal Antibody (NBP2-59315) at 1:1000 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~200 kDa.")
![Immunocytochemistry/Immunofluorescence: Ankyrin 1 Antibody (S388A-10) [NBP2-59315] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Ankyrin 1 Monoclonal Antibody, Clone S388A-10 (NBP2-59315). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Ankyrin 1 Monoclonal Antibody (NBP2-59315) at 1:100 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Ankyrin 1 Antibody (D) Composite.](http://images.novusbio.com/fullsize/Ankyrin-1-Antibody-S388A-10-Immunocytochemistry-Immunofluorescence-NBP2-59315-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Ankyrin 1 Antibody (S388A-10) [NBP2-59315] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Ankyrin R Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm, Nucleus. Magnification: 60X.](http://images.novusbio.com/fullsize/Ankyrin-1-Antibody-S388A-10-Immunocytochemistry-Immunofluorescence-NBP2-59315-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Ankyrin Brain Antibody (S105-13) [NBP2-59374] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Ankyrin Brain Monoclonal Antibody, Clone S105-13 (NBP2-59374). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Ankyrin Brain Monoclonal Antibody (NBP2-59374) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Ankyrin Brain Antibody (D) Composite.](http://images.novusbio.com/fullsize/Ankyrin-Brain-Antibody-S105-13-Immunocytochemistry-Immunofluorescence-NBP2-59374-img0003.jpg "Immunocytochemistry/Immunofluorescence: Ankyrin Brain Antibody (S105-13) [NBP2-59374] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Ankyrin Brain Monoclonal Antibody, Clone S105-13 (NBP2-59374). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Ankyrin Brain Monoclonal Antibody (NBP2-59374) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Ankyrin Brain Antibody (D) Composite.")
![Immunocytochemistry/Immunofluorescence: Ankyrin Brain Antibody (S105-13) [NBP2-59374] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Ankyrin B Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm](http://images.novusbio.com/fullsize/Ankyrin-Brain-Antibody-S105-13-Immunocytochemistry-Immunofluorescence-NBP2-59374-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Ankyrin Brain Antibody (S105-13) [NBP2-59374] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Ankyrin Brain Monoclonal Antibody, Clone S105-13 (NBP2-59374). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Ankyrin Brain Monoclonal Antibody (NBP2-59374) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm](http://images.novusbio.com/fullsize/Ankyrin-Brain-Antibody-S105-13-Immunocytochemistry-Immunofluorescence-NBP2-59374-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Ankyrin 3 Antibody [NBP3-05550] - Western blot analysis of different tissue lysates using Ankyrin 3 Antibody at a dilution of 1:1,000 in green: [1] protein standard (red), [2] rat cortex, [3] rat cortex membrane enriched fraction, [4] mouse cortex and [5] mouse cortex membrane enriched fraction. The band at ~190kDa correspond to one of the three high molecular weight forms of Ankyrin 3, the 270kDa and 480kDa isoforms can be seen on longer exposure of the blot.](http://images.novusbio.com/fullsize/Ankyrin-3-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05550-img0002.jpg "Immunocytochemistry/Immunofluorescence: Ankyrin 3 Antibody [NBP3-05550] - Western blot analysis of different tissue lysates using Ankyrin 3 Antibody at a dilution of 1:1,000 in green: [1] protein standard (red), [2] rat cortex, [3] rat cortex membrane enriched fraction, [4] mouse cortex and [5] mouse cortex membrane enriched fraction. The band at ~190kDa correspond to one of the three high molecular weight forms of Ankyrin 3, the 270kDa and 480kDa isoforms can be seen on longer exposure of the blot.")
![Immunocytochemistry/Immunofluorescence: Ankyrin 3 Antibody [NBP3-05550] - Immunofluorescent analysis of a rat brain section stained with Ankyrin 3 Antibody at a dilution of 1:1,000, in red and costained with mouse mAb to MAP2 at a dilution of 1:5,000 in green. The blue is Hoechst staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. The Ankyrin 3 antibody stains axonal initial segments, while the MAP2 antibody labels MAP2 protein expressed in the perikarya and dendrites of most neurons.](http://images.novusbio.com/fullsize/Ankyrin-3-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05550-img0001.jpg)
![Immunohistochemistry-Paraffin: Ankyrin Brain Antibody [NBP2-34023] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/Ankyrin-Brain-Antibody-Immunohistochemistry-Paraffin-NBP2-34023-img0003.jpg "Immunohistochemistry-Paraffin: Ankyrin Brain Antibody [NBP2-34023] - Staining of human pancreas shows low expression as expected.")
![Immunohistochemistry-Paraffin: Ankyrin Brain Antibody [NBP2-34023] - Staining of human cerebral cortex shows high expression.](http://images.novusbio.com/fullsize/Ankyrin-Brain-Antibody-Immunohistochemistry-Paraffin-NBP2-34023-img0002.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Ankyrin Brain Antibody [NBP2-34023] - Staining in human cerebral cortex and pancreas tissues using anti-ANK2 antibody. Corresponding ANK2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Ankyrin-Brain-Antibody-Immunohistochemistry-Paraffin-NBP2-34023-img0004.jpg)
![Western Blot: Ankyrin 3 Antibody [NBP2-58275] - Analysis in human cell line SK-BR-3.](http://images.novusbio.com/fullsize/Ankyrin-3-Antibody-Western-Blot-NBP2-58275-img0002.jpg "Western Blot: Ankyrin 3 Antibody [NBP2-58275] - Analysis in human cell line SK-BR-3.")
![Immunocytochemistry/Immunofluorescence: Ankyrin 3 Antibody [NBP2-58275] - Staining of human cell line U-2 OS shows localization to plasma membrane.](http://images.novusbio.com/fullsize/Ankyrin-3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-58275-img0001.jpg)