| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35084-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-94176-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-97576-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-42187-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-37975-20ul | |||
![Western Blot: VDAC1 Antibody [NB100-695] - The BNIP3 C-terminus can be phosphorylated. Subcellular localization of BNIP3 phosphomutants, showing Western blot of cytosolic and mitochondrial fractions. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0129667), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/VDAC1-Antibody-Western-Blot-NB100-695-img0005.jpg "Western Blot: VDAC1 Antibody [NB100-695] - The BNIP3 C-terminus can be phosphorylated. Subcellular localization of BNIP3 phosphomutants, showing Western blot of cytosolic and mitochondrial fractions. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0129667), licensed under a CC-BY license.")
![Western Blot: VDAC1 Antibody [NB100-695] - Lane 1: rat heart whole cell lysate. Lane 2: none. Load: 35 ug per lane. Primary antibody: VDAC/Porin antibody at 1:1,200 for overnight at 4C. Secondary antibody: IRDye800 (TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: ~32 kDa corresponding to VDAC/Porin (arrowhead).](http://images.novusbio.com/fullsize/VDAC1-Antibody-Western-Blot-NB100-695-img0004.jpg)
![Western Blot: VDAC1 Antibody [NB100-695] - The BNIP3 C-terminus can be phosphorylated. Alkaline extraction of mitochondria-associated proteins, showing alkaline extract of the mitochondrial pellet and the alkaline-resistant mitochondrial pellet from cells expressing each BNIP3 phosphomutant. All blots are representative of at least 3 independent experiments. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0129667), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/VDAC1-Antibody-Western-Blot-NB100-695-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: VDAC2 Antibody [NBP2-20849] - Confocal immunofluorescence analysis of methanol-fixed HeLa, using VDAC2 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:2000.](http://images.novusbio.com/fullsize/VDAC2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-20849-img0003.jpg "Immunocytochemistry/Immunofluorescence: VDAC2 Antibody [NBP2-20849] - Confocal immunofluorescence analysis of methanol-fixed HeLa, using VDAC2 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:2000.")
![Western Blot: VDAC2 Antibody [NBP2-20849] - Analysis in mouse and rat eye samples (upper panel). blot was reprobed with actin antibody (lower panel). Image from a verified customer review.](http://images.novusbio.com/fullsize/VDAC2-Antibody-Western-Blot-NBP2-20849-img0009.jpg)
![Immunohistochemistry-Paraffin: VDAC2 Antibody [NBP2-20849] - Rat kidney. VDAC2 antibody [C2C3], C-term diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/VDAC2-Antibody-Immunohistochemistry-Paraffin-NBP2-20849-img0005.jpg)
![Immunohistochemistry-Paraffin: VDAC2 Antibody [NBP2-20849] - Mouse intestine. VDAC2 antibody [C2C3], C-term diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/VDAC2-Antibody-Immunohistochemistry-Paraffin-NBP2-20849-img0006.jpg)
![Western Blot: VDAC2 Antibody [NBP2-20849] - Various tissue extracts (50 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with VDAC2 antibody [C2C3], C-term (NBP2-20849) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-20849_rabbit-polyclonal-vdac2-antibody-9102024205698.jpg)
![VDAC2 antibody [C2C3], C-term detects VDAC2 protein at mitochondria by immunohistochemical analysis.Sample: Paraffin-embedded mouse kidney.VDAC2 stained by VDAC2 antibody [C2C3], C-term (NBP2-20849) diluted at 1:500.Antigen Retrieval: Citrat](http://images.novusbio.com/fullsize/nbp2-20849_rabbit-polyclonal-vdac2-antibody-immunohistochemistry-paraffin-261120251845132.jpg)
![Mouse tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with VDAC2 antibody [C2C3], C-term (NBP2-20849) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-20849_rabbit-polyclonal-vdac2-antibody-western-blot-261120251837245.jpg)
![Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with VDAC2 antibody [C2C3], C-term (NBP2-20849) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-20849_rabbit-polyclonal-vdac2-antibody-western-blot-261120251826365.jpg)
![Western Blot: VDAC1 Antibody (6B1K1) [VDAC1] - Western blot analysis of various lysates using VDAC1 Rabbit mAb at 1:5000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 15s.](http://images.novusbio.com/fullsize/nbp3-15871_rabbit-vdac1-mab-6b1k1-5220251718924.jpg "Western Blot: VDAC1 Antibody (6B1K1) [VDAC1] - Western blot analysis of various lysates using VDAC1 Rabbit mAb at 1:5000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 15s.")
![Immunohistochemistry-Paraffin: VDAC1 Antibody (6B1K1) [NBP3-15871] - Human colon carcinoma tissue using VDAC1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15871_rabbit-vdac1-mab-6b1k1-immunohistochemistry-paraffin-492024102517..jpg)
![Immunohistochemistry-Paraffin: VDAC1 Antibody (6B1K1) [NBP3-15871] -Human thyroid cancer tissue using VDAC1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15871_rabbit-vdac1-mab-6b1k1-immunohistochemistry-paraffin-492024102714..jpg)
![Immunohistochemistry-Paraffin: VDAC1 Antibody (6B1K1) [NBP3-15871] -Mouse kidney tissue using VDAC1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15871_rabbit-vdac1-mab-6b1k1-immunohistochemistry-paraffin-492024102816..jpg)
![Immunocytochemistry/ Immunofluorescence: VDAC1 Antibody (6B1K1) [VDAC1] - Confocal imaging of NIH/3T3 cells using VDAC1 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-15871_rabbit-vdac1-mab-6b1k1-52202517162313.jpg)
![Immunocytochemistry/ Immunofluorescence: VDAC1 Antibody (6B1K1) [VDAC1] - Confocal imaging of HeLa cells using VDAC1 Rabbit mAb . The cells were counterstained with alpha-Tubulin Mouse mAb (Green). DAPI was used for nuclear staining (blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-15871_rabbit-vdac1-mab-6b1k1-52202517223937.jpg)
![Immunohistochemistry: VDAC1 Antibody (6B1K1) [VDAC1] - Immunohistochemistry analysis of paraffin-embedded Human liver tissue using VDAC1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15871_rabbit-vdac1-mab-6b1k1-5220251715272.jpg)
![Immunohistochemistry: VDAC1 Antibody (6B1K1) [VDAC1] - Immunohistochemistry analysis of paraffin-embedded Rat kidney tissue using VDAC1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15871_rabbit-vdac1-mab-6b1k1-52202517213578.jpg)
![Immunohistochemistry-Paraffin: VDAC2 Antibody [NBP1-89477] - Staining of human endometrium shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/VDAC2-Antibody-Immunohistochemistry-Paraffin-NBP1-89477-img0013.jpg "Immunohistochemistry-Paraffin: VDAC2 Antibody [NBP1-89477] - Staining of human endometrium shows strong cytoplasmic positivity in glandular cells.")
![Immunohistochemistry-Paraffin: VDAC2 Antibody [NBP1-89477] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/VDAC2-Antibody-Immunohistochemistry-Paraffin-NBP1-89477-img0010.jpg)
![Immunohistochemistry-Paraffin: VDAC2 Antibody [NBP1-89477] - Staining of human heart muscle shows strong cytoplasmic positivity in cardiomyocytes.](http://images.novusbio.com/fullsize/VDAC2-Antibody-Immunohistochemistry-Paraffin-NBP1-89477-img0011.jpg)
![Immunohistochemistry-Paraffin: VDAC2 Antibody [NBP1-89477] - Staining of human cerebellum shows strong cytoplasmic positivity in purkinje cells.](http://images.novusbio.com/fullsize/VDAC2-Antibody-Immunohistochemistry-Paraffin-NBP1-89477-img0012.jpg)
![Western Blot: VDAC2 Antibody (3D2) [H00007417-M01] - VDAC2 monoclonal antibody (M01), clone 3D2. Analysis of VDAC2 expression in rat brain.](http://images.novusbio.com/fullsize/VDAC2-Antibody-3D2-Western-Blot-H00007417-M01-img0006.jpg "Western Blot: VDAC2 Antibody (3D2) [H00007417-M01] - VDAC2 monoclonal antibody (M01), clone 3D2. Analysis of VDAC2 expression in rat brain.")
![Immunocytochemistry/Immunofluorescence: VDAC2 Antibody (3D2) [H00007417-M01] - Analysis of monoclonal antibody to VDAC2 on HeLa cell . Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/VDAC2-Antibody-3D2-Immunocytochemistry-Immunofluorescence-H00007417-M01-img0003.jpg)
![Western Blot: VDAC2 Antibody (3D2) [H00007417-M01] - VDAC2 monoclonal antibody (M01), clone 3D2. Analysis of VDAC2 expression in K-562.](http://images.novusbio.com/fullsize/VDAC2-Antibody-3D2-Western-Blot-H00007417-M01-img0005.jpg)
![Western Blot: VDAC2 Antibody (3D2) [H00007417-M01] - Analysis of VDAC2 expression in transfected 293T cell line by VDAC2 monoclonal antibody (M01), clone 3D2.Lane 1: VDAC2 transfected lysate(31.6 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/VDAC2-Antibody-3D2-Western-Blot-H00007417-M01-img0007.jpg)
![Sandwich ELISA: VDAC2 Antibody (3D2) [H00007417-M01] - Detection limit for recombinant GST tagged VDAC2 is 10 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/VDAC2-Antibody-3D2-Sandwich-ELISA-H00007417-M01-img0004.jpg)
![Western Blot: VDAC3 Antibody [NBP1-80070] - Human Fetal Muscle, Antibody Dilution: 1.0 ug/ml.](http://images.novusbio.com/fullsize/VDAC3-Antibody-Western-Blot-NBP1-80070-img0006.jpg "Western Blot: VDAC3 Antibody [NBP1-80070] - Human Fetal Muscle, Antibody Dilution: 1.0 ug/ml.")
![Immunohistochemistry: VDAC3 Antibody [NBP1-80070] - Human Lung cell Cellular data: Epithelial cells of renal tubule Antibody Concentration: 4.0-8.0 ug/ml Magnification: 400X.](http://images.novusbio.com/fullsize/VDAC3-Antibody-Immunohistochemistry-NBP1-80070-img0003.jpg)
![Western Blot: VDAC3 Antibody [NBP1-80070] - Human Heart lysate, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/VDAC3-Antibody-Western-Blot-NBP1-80070-img0002.jpg)
![Western Blot: VDAC3 Antibody [NBP1-80070] - Analysis of 721_B cell lysate. Antibody Dilution: 1.0 ug/ml VDAC3 is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells.](http://images.novusbio.com/fullsize/VDAC3-Antibody-Western-Blot-NBP1-80070-img0004.jpg)
![Western Blot: VDAC3 Antibody [NBP1-80070] - Human Fetal Heart, Antibody Dilution: 1.0 ug/ml.](http://images.novusbio.com/fullsize/VDAC3-Antibody-Western-Blot-NBP1-80070-img0005.jpg)
![Immunohistochemistry: VDAC1 Antibody [NBP3-35084] - Immunohistochemistry analysis of paraffin-embedded Rat liver using VDAC1 Rabbit pAb at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35084_rabbit-vdac1-pab-101220241136039.jpg "Immunohistochemistry: VDAC1 Antibody [NBP3-35084] - Immunohistochemistry analysis of paraffin-embedded Rat liver using VDAC1 Rabbit pAb at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.")
![Western Blot: VDAC1 Antibody [NBP3-35084] - Western blot analysis of various lysates using VDAC1 Rabbit pAb at 1:400 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.](http://images.novusbio.com/fullsize/nbp3-35084_rabbit-vdac1-pab-1012202411392971.jpg)
![Western Blot: VDAC1 Antibody [NBP3-35084] - Western blot analysis of various lysates using VDAC1 Rabbit pAb at 1:400 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-35084_rabbit-vdac1-pab-1012202411353068.jpg)
![Immunohistochemistry: VDAC1 Antibody [NBP3-35084] - Immunohistochemistry analysis of paraffin-embedded Human liver using VDAC1 Rabbit pAb at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35084_rabbit-vdac1-pab-1012202411353077.jpg)
![Immunohistochemistry: VDAC1 Antibody [NBP3-35084] - Immunohistochemistry analysis of paraffin-embedded Mouse heart using VDAC1 Rabbit pAb at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35084_rabbit-vdac1-pab-1012202411381232.jpg)
![Western Blot: VDAC2 Antibody [NB300-871] - Staining (1ug/ml) of Human Cerebellum lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/VDAC2-Antibody-Western-Blot-NB300-871-img0002.jpg "Western Blot: VDAC2 Antibody [NB300-871] - Staining (1ug/ml) of Human Cerebellum lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: VDAC2 Antibody [NB300-871] - Staining (6ug/ml) of paraffin embedded Rat Liver. Heat induced antigen retrieval with citrate buffer pH 6, HRP-Staining.](http://images.novusbio.com/fullsize/VDAC2-Antibody-Immunohistochemistry-Paraffin-NB300-871-img0003.jpg)
![Immunohistochemistry-Paraffin: VDAC2 Antibody [NB300-871] - Negative Control showing staining of paraffin embedded Rat Liver, with no primary antibody.](http://images.novusbio.com/fullsize/VDAC2-Antibody-Immunohistochemistry-Paraffin-NB300-871-img0004.jpg)
![Western Blot: VDAC3 Antibody [NBP2-94176] - Analysis of extracts of Mouse heart, using VDAC3 antibody (NBP2-94176) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/VDAC3 Antibody-Western Blot-NBP2-94176-img0004.jpg "Western Blot: VDAC3 Antibody [NBP2-94176] - Analysis of extracts of Mouse heart, using VDAC3 antibody (NBP2-94176) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.")
![Immunohistochemistry-Paraffin: VDAC3 Antibody [NBP2-94176] - Mouse lung using VDAC3 Rabbit pAb (NBP2-94176) at dilution of 1:25 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/VDAC3-Antibody-Immunohistochemistry-Paraffin-NBP2-94176-img0003.jpg)
![Immunohistochemistry-Paraffin: VDAC3 Antibody [NBP2-94176] - Mouse kidney using VDAC3 Rabbit pAb (NBP2-94176) at dilution of 1:25 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/VDAC3-Antibody-Immunohistochemistry-Paraffin-NBP2-94176-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: VDAC3 Antibody - Azide and BSA Free [VDAC3] - Immunofluorescence analysis of HeLa cells using VDAC3 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-94176_rabbit-polyclonal-vdac3-antibody-5220251628068.jpg)
![Western Blot: VDAC2 Antibody [NBP2-97576] - Anti-VDAC2 rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate Lane B: HepG2 Whole Cell Lysate Lane C: HCT116 Whole Cell Lysate Lane D: U-251 MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 31 kDa. Observed band size: 34 kDa](http://images.novusbio.com/fullsize/VDAC2-Antibody-Western-Blot-NBP2-97576-img0002.jpg "Western Blot: VDAC2 Antibody [NBP2-97576] - Anti-VDAC2 rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate Lane B: HepG2 Whole Cell Lysate Lane C: HCT116 Whole Cell Lysate Lane D: U-251 MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 31 kDa. Observed band size: 34 kDa")
![Immunohistochemistry-Paraffin: VDAC2 Antibody [NBP2-97576] - Immunochemical staining of human VDAC2 in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/VDAC2-Antibody-Immunohistochemistry-Paraffin-NBP2-97576-img0005.jpg)
![Immunohistochemistry-Paraffin: VDAC2 Antibody [NBP2-97576] - Immunochemical staining of human VDAC2 in cynomolgus cerebellum with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/VDAC2-Antibody-Immunohistochemistry-Paraffin-NBP2-97576-img0003.jpg)
![Immunohistochemistry-Paraffin: VDAC2 Antibody [NBP2-97576] - Immunochemical staining of human VDAC2 in cynomolgus heart with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/VDAC2-Antibody-Immunohistochemistry-Paraffin-NBP2-97576-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: VDAC2 Antibody [NBP2-97576] - Immunofluorescence staining of VDAC2 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-97576 antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.](http://images.novusbio.com/fullsize/nbp2-97576_rabbit-polyclonal-vdac2-antibody-235202511444435.png)
![Western Blot: VDAC1 Antibody (S152B-23) [NBP2-42187] - Western Blot analysis of Mouse Brain and Human RT-4 lysates showing detection of ~30 kDa VDAC1 protein using Mouse Anti-VDAC1 Monoclonal Antibody, Clone S152B-23 (NBP2-42187). Lane 1: Molecular Weight Ladder. Lane A: Mouse Brain Lysate. Lane B: Human RT-4 Lysate. Block: 5% Skim milk in TBST . Primary Antibody: Mouse Anti-VDAC1 Monoclonal Antibody (NBP2-42187) at 1:200 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: ~30 kDa.](http://images.novusbio.com/fullsize/VDAC1-Antibody-S152B-23-Western-Blot-NBP2-42187-img0004.jpg "Western Blot: VDAC1 Antibody (S152B-23) [NBP2-42187] - Western Blot analysis of Mouse Brain and Human RT-4 lysates showing detection of ~30 kDa VDAC1 protein using Mouse Anti-VDAC1 Monoclonal Antibody, Clone S152B-23 (NBP2-42187). Lane 1: Molecular Weight Ladder. Lane A: Mouse Brain Lysate. Lane B: Human RT-4 Lysate. Block: 5% Skim milk in TBST . Primary Antibody: Mouse Anti-VDAC1 Monoclonal Antibody (NBP2-42187) at 1:200 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: ~30 kDa.")
![Immunocytochemistry/Immunofluorescence: VDAC1 Antibody (S152B-23) [NBP2-42187] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-VDAC1 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Mitochondrion, Mitochondrion Outer Membrane, Nucleus. Magnification: 60X.](http://images.novusbio.com/fullsize/VDAC1-Antibody-S152B-23-Immunocytochemistry-Immunofluorescence-NBP2-42187-img0002.jpg)
![Western Blot: VDAC1 Antibody (S152B-23) [NBP2-42187] - Western Blot analysis of Rat Brain Membrane showing detection of ~30 kDa VDAC1 protein using Mouse Anti-VDAC1 Monoclonal Antibody, Clone S152B-23 (NBP2-42187). Lane 1: Molecular Weight Ladder. Lane 2: Rat Brain Membrane. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-VDAC1 Monoclonal Antibody (NBP2-42187) at 1:200 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~30 kDa.](http://images.novusbio.com/fullsize/VDAC1-Antibody-S152B-23-Western-Blot-NBP2-42187-img0003.jpg)
![Western Blot: VDAC1 Antibody [NBP3-37975] - Western blot analysis of various lysates, using [KD Validated] VDAC1 Rabbit pAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-37975_rabbit-vdac1-pab-10122024121053.jpg "Western Blot: VDAC1 Antibody [NBP3-37975] - Western blot analysis of various lysates, using [KD Validated] VDAC1 Rabbit pAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.")
![Western Blot: VDAC1 Antibody [NBP3-37975] - Western blot analysis of various lysates, using [KD Validated] VDAC1 Rabbit pAb at 1:600 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-37975_rabbit-vdac1-pab-10122024121017.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: VDAC1 Antibody [NBP3-37975] - Western Blot analysis of lysates from wild type (WT) and VDAC1 knockdown (KD) 293T cells, using [KD Validated] VDAC1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.](http://images.novusbio.com/fullsize/nbp3-37975_rabbit-vdac1-pab-311202517707.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: VDAC1 Antibody [NBP3-37975] - Western Blot analysis of lysates from wild type(WT) and VDAC1 knockdown (KD) 293T cells, using [KD Validated] VDAC1 Rabbit pAb at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-37975_rabbit-vdac1-pab-3112025173156.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: VDAC1 Antibody [NBP3-37975] - Western Blot analysis of lysates from wild type(WT) and VDAC1 knockdown (KD) 293T cells, using [KD Validated] VDAC1 Rabbit pAb at 1:600 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-37975_rabbit-vdac1-pab-31120251744490.jpg)