HAF016). A specific band was detected for CD94 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8." class="big_lightbox"> HAF016). A specific band was detected for CD94 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8." alt="Western blot shows lysates of CLL-1390 canine leukocytic round cell neoplasia cell line, HDLM human Hodgkin’s lymphoma cell line, and CTLL-2 mouse cytotoxic T cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human CD94 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1058) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CD94 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8." class="big_thumb" />

9759-NK) is immobilized at 2 µg/mL (100 µL/well), Recombinant Human CD94 Fc Chimera (Catalog # 10281-CD) binds with an ED₅₀ of 1.25-10 µg/mL." class="big_lightbox"> 9759-NK) is immobilized at 2 µg/mL (100 µL/well), Recombinant Human CD94 Fc Chimera (Catalog # 10281-CD) binds with an ED₅₀ of 1.25-10 µg/mL." alt="When Recombinant Human NKG2E Fc Chimera (9759-NK) is immobilized at 2 µg/mL (100 µL/well), Recombinant Human CD94 Fc Chimera (Catalog # 10281-CD) binds with an ED₅₀ of 1.25-10 µg/mL." class="big_thumb" />

9198-NK) is coated at 0.5 µg/mL, Recombinant Human CD94 (Catalog # 9270-CD) binds with an ED₅₀ of 0.1-0.6 µg/mL." class="big_lightbox"> 9198-NK) is coated at 0.5 µg/mL, Recombinant Human CD94 (Catalog # 9270-CD) binds with an ED₅₀ of 0.1-0.6 µg/mL." alt="When Recombinant Mouse NKG2A Fc Chimera (Catalog # 9198-NK) is coated at 0.5 µg/mL, Recombinant Human CD94 (Catalog # 9270-CD) binds with an ED₅₀ of 0.1-0.6 µg/mL." class="big_thumb" />

MAB0041) followed by anti-Mouse IgG Allophycocyanin-conjugated secondary antibody (F0101B) and Mouse Anti-Human CD56 Phycoerythrin-conjugated Monoclonal Antibody (FAB24086P). Staining was performed using our Staining Membrane-associated Proteins protocol." class="big_lightbox"> MAB0041) followed by anti-Mouse IgG Allophycocyanin-conjugated secondary antibody (F0101B) and Mouse Anti-Human CD56 Phycoerythrin-conjugated Monoclonal Antibody (FAB24086P). Staining was performed using our Staining Membrane-associated Proteins protocol." alt="Human peripheral blood lymphocytes were stained with (A) Mouse Anti-Human CD94 Monoclonal Antibody (Catalog # MAB10581) or (B) Mouse IgG2B isotype control antibody (MAB0041) followed by anti-Mouse IgG Allophycocyanin-conjugated secondary antibody (F0101B) and Mouse Anti-Human CD56 Phycoerythrin-conjugated Monoclonal Antibody (FAB24086P). Staining was performed using our Staining Membrane-associated Proteins protocol." class="big_thumb" />