| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF2724-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF3355-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF2554-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF3414-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB27243-SP | |||
| Carrier Free | MAB27243-500 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB2554-SP | |||
![Western Blot: CD200/OX2 Antibody (PSH01-48) [NBP3-32132] - Western blot analysis of CD200/OX2 on different lysates with Rabbit anti-CD200/OX2 antibody (NBP3-32132) at 1/1,000 dilution. Lane 1: SK-MEL-28 cell lysate (20 ug/Lane) Lane 2: NCI-H226 cell lysate (20 ug/Lane) Lane 3: Human brain tissue lysate (30 ug/Lane) Lane 4: Human placenta tissue lysate (30 ug/Lane) Lane 5: Human lung tissue lysate (30 ug/Lane) Predicted band size: 31 kDa Observed band size: 40-50kDa Exposure time: 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32132) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32132_rabbit-cd200-ox2-mab-psh01-48-278202415295558.jpg "Western Blot: CD200/OX2 Antibody (PSH01-48) [NBP3-32132] - Western blot analysis of CD200/OX2 on different lysates with Rabbit anti-CD200/OX2 antibody (NBP3-32132) at 1/1,000 dilution. Lane 1: SK-MEL-28 cell lysate (20 ug/Lane) Lane 2: NCI-H226 cell lysate (20 ug/Lane) Lane 3: Human brain tissue lysate (30 ug/Lane) Lane 4: Human placenta tissue lysate (30 ug/Lane) Lane 5: Human lung tissue lysate (30 ug/Lane) Predicted band size: 31 kDa Observed band size: 40-50kDa Exposure time: 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32132) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: CD200/OX2 Antibody (PSH01-48) [NBP3-32132] - Immunofluorescence analysis of paraffin-embedded human tonsil tissue labeling CD200/OX2 with Rabbit anti-CD200/OX2 antibody (NBP3-32132) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32132, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32132_rabbit-cd200-ox2-mab-psh01-48-298202414333642.jpg)
![Immunohistochemistry: CD200/OX2 Antibody (PSH01-48) [NBP3-32132] - Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-CD200/OX2 antibody (NBP3-32132) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32132) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32132_rabbit-cd200-ox2-mab-psh01-48-298202414304113.jpg)
![Flow Cytometry: CD200/OX2 Antibody (PSH01-48) [NBP3-32132] - Flow cytometric analysis of SK-MEL-28 cells labeling CD200/OX2. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (NBP3-32132, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32132_rabbit-cd200-ox2-mab-psh01-48-29202416453219.jpg)