ZBTB8A Antibody - BSA Free

Western Blot: ZBTB8A Antibody [NBP1-76521] - Mouse spleen tissue lysate with ZBTB8 antibody at 1 ug/mL.

Immunocytochemistry/Immunofluorescence: ZBTB8A Antibody [NBP1-76521] - Immunofluorescence of ZBTB8 in human spleen tissue with ZBTB8 antibody at 20 ug/mL.

Immunohistochemistry: ZBTB8A Antibody [NBP1-76521] - Immunohistochemistry of ZBTB8 in human spleen tissue with ZBTB9 antibody at 2.5 ug/mL.

• Reactivity: Hu, Mu, Rt, Bv
• Applications: WB, ELISA, ICC/IF, IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

ZBTB8A Antibody - BSA Free Summary

• Immunogen: Antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human ZBTB8. The immunogen is located within the last 50 amino acids of ZBTB8. Amino Acid Squence: VDDSEEEEEKEIKPNIR
• Specificity: At least two isoforms of ZBTB8A are known to exist; this antibody will recognize both isoforms. This antibody is predicted to not cross-react with other ZBTB protein family members.
• Predicted Species: Rat (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ZBTB8A
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 5 ug/ml
  • Immunohistochemistry 2.5 ug/ml
  • Immunohistochemistry-Paraffin 2.5 ug/ml
  • Western Blot 1 ug/ml
• Control Peptide:

  ZBTB8A Protein (NBP1-76521PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for ZBTB8A Antibody - BSA Free

• BOZ-F1
• BOZF1BTB/POZ and zinc-finger domains factor on chromosome 1
• BTB/POZ and zinc-finger domain-containing factor
• FLJ90065
• MGC17919
• ZBTB8
• zinc finger and BTB domain containing 8
• zinc finger and BTB domain containing 8A
• zinc finger and BTB domain-containing protein 8A
• ZNF916A

Background

The ZBTB family of proteins is comprised of diverse zinc finger proteins that also contain a BTB (BR-C, ttk and bab) domain. ZBTB8, also known as BOZ-F1, is present in multiple tissues, and is highly expressed in the cytoplasm of the placenta. While little is known about ZBTB8, other ZBTB proteins, such as ZBTB4 bind methylated DNA and repress transcription. Another ZBTB proteins, ZBTB7A, has been implicated as a proto-oncogene whose overexpression contributes to malignancy in breast cancer, suggesting that ZBTB8 may act as a transcriptional repressor or be involved in tumorigenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ZBTB8A
• Uniprot:
  • CAI20024(Human)