WW domain binding protein 5 Overexpression Lysate (Native)

Images

 
Western Blot: WW domain binding protein 5 Overexpression Lysate (Adult Normal) [NBL1-17783] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for WW domain binding ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

WW domain binding protein 5 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC212064 and based on accession number NM_016303. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens WW domain binding protein 5 (WBP5), transcript variant 1, mRNA.
Gene
WBP5

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
12.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for WW domain binding protein 5

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for WW domain binding protein 5 Overexpression Lysate (Native)

  • DKFZp313K1940
  • N4
  • NDFIP1
  • pp21 homolog
  • TCEAL9
  • WBP-5
  • WW domain binding protein 1
  • WW domain binding protein 5
  • WW domain-binding protein 5

Background

The globular WW domain is composed of 38 to 40 semiconserved amino acids shared by proteins of diverse functions including structural, regulatory, and signaling proteins. The domain is involved in mediating protein-protein interactions through the binding of polyproline ligands. This gene encodes a WW domain binding protein. This gene also encodes a domain with similarity to the transcription elongation factor A, SII-related family. Alternative splicing results in multiple transcript variants encoding a single isoform. Transcript Variant: This variant (1) represents the longest transcript. Variants 1 through 4 encode the same isoform.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Product General Protocols

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Video Protocols

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Bioinformatics

Gene Symbol WBP5