WNVM Antibody

Images

 
Western Blot: WNVM Antibody [NB100-56743] - Analysis of West Nile Virus glycoprotein M in (A) untransfected and (B) transfected mouse melanoma cells using NB100-56743 at 1 ug/ml.
Immunohistochemistry-Paraffin: WNVM Antibody [NB100-56743] - Analysis of WNV-Envelope protein in bird liver using this antibody at 1 ug/ml, incubated for 30 minutes. A Ventana brand second step antibody conjugated to ...read more

Product Details

Summary
Reactivity Hu, Mu, Ha, Pm, V-ViSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

WNVM Antibody Summary

Immunogen
A synthetic peptide corresponding to amino acid residues 8-27 (GESTLANKKGAWLDSTKATR) of West Nile Virus glycoprotein M. Genbank accession no. NP_776013.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5-2 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:20-1:1000
  • Immunohistochemistry 1:20-1:1000
  • Immunohistochemistry-Paraffin 1.0 ug/ ml
  • Immunoprecipitation 1:20-1:1000
Application Notes
Immunocytochemistry/Immunofluorescence: 1-2 ug/ml, see Tan et al (2009) for details. Immunoprecipitation: see Tan et al (2009) for details.
Publications
Read Publications using
NB100-56743 in the following applications:

  • WB
    10 publications

Reactivity Notes

West Nile Virus glycoprotein M (Genbank accession no. NP_776013). Monkey, human, and hamster reactivity reported in scientific literature (PMID: 27383988). Mouse reactivity reported in scientific literature (PMID: 28187142).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Affinity purified

Alternate Names for WNVM Antibody

  • West Nile Virus envelope glycoprotein M

Background

West Nile (WN), the most widespread among flaviviruses, was first isolated from the serum of a febrile woman in 1937 in the West Nile district of Uganda. West Nile virus was first detected in North America in 1999 and has subsequently spread throughout the United States and Canada and into Mexico and the Caribbean. In Africa, southern Europe, western Asia, and the United States, WNV has been isolated from mosquitoes of more than 40 species. In the United States, Canada, and Israel, WNV is responsible for significant avian mortality.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Publications for WNVM Antibody (NB100-56743)(11)

We have publications tested in 5 confirmed species: Human, Hamster, Monkey, Virus, mouse.

We have publications tested in 1 application: WB.


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WB
(10)
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Human
(3)
Hamster
(1)
Monkey
(1)
Virus
(3)
mouse
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Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using NB100-56743 Applications Species
Sasaki M, Anindita PD, Phongphaew W et al. Development of a rapid and quantitative method for the analysis of viral entry and release using a NanoLuc luciferase complementation assay. Virus Res. 2017 Oct 23 [PMID: 29074234] (WB, Human) WB Human
Boylan BT, Moreira FR, Carlson TW, Bernard KA. Mosquito cell-derived West Nile virus replicon particles mimic arbovirus inoculum and have reduced spread in mice. PLoS Negl Trop Dis. 2017 Feb 01 [PMID: 28187142] (WB, mouse) WB mouse
Zhang R, Miner JJ, Gorman MJ et al. A CRISPR screen defines a signal peptide processing pathway required by flaviviruses Nature. Jul 7 2016 [PMID: 27383988] (WB, Monkey, Human, Hamster) WB Monkey, Human, Hamster
Merino-Ramos T, Blazquez AB, Escribano-Romero E et al. Protection of a Single Dose West Nile Virus Recombinant Subviral Particle Vaccine against Lineage 1 or 2 Strains and Analysis of the Cross-Reactivity with Usutu Virus. PLoS ONE. 2014 Sep 18 [PMID: 25229345] (WB, Virus)

Details:
WNVM antibody used for WB analysis of WNV proteins released to the culture medium from HeLa or 293T cells transfected with plasmid pcDNA-WNV, and a control lane containing purified WNV from WNV-infected Vero cells was included in th assay.
WB Virus
Martin-Acebes MA, Merino-Ramos T, Blazquez AB et al. The Composition of West Nile virus Lipid Envelope Unveils a Role of Sphingolipid Metabolism on Flavivirus Biogenesis. J Virol. 2014 Aug 13 [PMID: 25122799] (WB, Virus)

Details:
WNVM antibody used for WB on lysates of HeLa or HeLa3-WNV cells. FIG 2E shows two bands - one band compatible with prM protein and the other band compatible with mature M protein (about 20 and 6 kDa, respectively).
WB Virus
Dowd KA, Mukherjee S, Kuhn RJ, Pierson TC. Combined effects of the structural heterogeneity and dynamics of flaviviruses on antibody recognition. J Virol. 2014 Jul 30 [PMID: 25078693] (WB, Virus)

Details:
For the evaluation of the efficiency of prM cleavage in WNV RVP preparations, WNVM Antibody used for WB at 1:250 dilution on aliquots of WNV-prM+ and WNV-furin RVPs that were partially purified by centrifugation through a 20% sucrose cushion followed by lysis in buffer containing 1% Triton, 100 mM Tris, 2 M NaCl, and 100 mM EDTA.
WB Virus
Makino Y, Suzuki T, Hasebe R et al. Establishment of tracking system for West Nile virus entry and evidence of microtubule involvement in particle transport. J Virol Methods 2014 Jan [PMID: 24140187] (WB, Human) WB Human
Lin TY, Dowd KA, Manhart CJ et al. A novel approach for the rapid mutagenesis and directed evolution of the structural genes of west nile virus. J Virol. 2012 Apr [PMID: 22258236] (WB)

Details:
WB: Wild type and mutant (N15A) West Nile Virus harvested from infected BHk-21 cells, Fig 4F.
WB
Tan TT, Bhuvanakantham R, Li J et al. Tyrosine 78 of premembrane protein is essential for assembly of West Nile virus. J Gen Virol. 2009 May [PMID: 19264649] (WB)

Details:
BHk-21 cells mock-infected or infected with WNV, or co-transfected with WNV M and E plasmids were used with the IMG-5099A WNV M pAb in the following techniques and figures: 1. IF (Figs 2, 6) 2. WB (Figs 4a, 4b, 8a, 8c (iii)
WB
Nelson S, Jost CA, Xu Q et al. Maturation of West Nile virus modulates sensitivity to antibody-mediated neutralization. PLoS Pathog. 2008 May 9 [PMID: 18464894]
Show All 11 Publications.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for WNVM Antibody (NB100-56743) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB100-56743AF350
Alexa Fluor 405 NB100-56743AF405
Alexa Fluor 488 NB100-56743AF488
Alexa Fluor 532 NB100-56743AF532
Alexa Fluor 594 NB100-56743AF594
Alexa Fluor 647 NB100-56743AF647
Alexa Fluor 700 NB100-56743AF700
Alexa Fluor 750 NB100-56743AF750
Biotin NB100-56743B
DyLight 350 NB100-56743UV
DyLight 405 NB100-56743V
DyLight 488 NB100-56743G
DyLight 550 NB100-56743R
DyLight 594 NB100-56743DL594
DyLight 650 NB100-56743C
DyLight 680 NB100-56743FR
DyLight 755 NB100-56743IR
FITC NB100-56743F
HRP NB100-56743H
Janelia Fluor 549 NB100-56743JF549
Janelia Fluor 646 NB100-56743JF646

Additional WNVM Products

Array NB100-56743

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