West Nile Virus MP Antibody - BSA Free


Western Blot: West Nile Virus MP Antibody - BSA Free [NB100-56743] - Analysis of West Nile Virus West Nile Virus MP in (A) untransfected and (B) transfected mouse melanoma cells using NB100-56743 at 1 ug/ml.
Immunohistochemistry-Paraffin: West Nile Virus MP Antibody - BSA Free [NB100-56743] - Analysis of West Nile Virus MP in bird liver using this antibody at 1 ug/ml, incubated for 30 minutes. A Ventana brand second step ...read more

Product Details

Reactivity V-ViSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
BSA Free
1.0 mg/ml

Order Details

West Nile Virus MP Antibody - BSA Free Summary

A synthetic peptide corresponding to amino acid residues 8-27 (GESTLANKKGAWLDSTKATR) of West Nile Virus MP. Genbank accession no. NP_776013.
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Immunocytochemistry/Immunofluorescence 1:20-1:1000
  • Immunohistochemistry 1:20-1:1000
  • Immunohistochemistry-Paraffin 1.0 ug/ ml
  • Immunoprecipitation 1:20-1:1000
  • Western Blot 0.5-2 ug/ml
Application Notes
Immunocytochemistry/Immunofluorescence: 1-2 ug/ml, see Tan et al (2009) for details. Immunoprecipitation: see Tan et al (2009) for details.
Read Publications using
NB100-56743 in the following applications:

  • WB
    10 publications

Reactivity Notes

West Nile Virus glycoprotein M (Genbank accession no. NP_776013).

Packaging, Storage & Formulations

Store at -20C. Avoid freeze-thaw cycles.
0.05% Sodium Azide
1.0 mg/ml
Affinity purified


West Nile (WN), the most widespread among flaviviruses, was first isolated from the serum of a febrile woman in 1937 in the West Nile district of Uganda. West Nile virus was first detected in North America in 1999 and has subsequently spread throughout the United States and Canada and into Mexico and the Caribbean. In Africa, southern Europe, western Asia, and the United States, WNV has been isolated from mosquitoes of more than 40 species. In the United States, Canada, and Israel, WNV is responsible for significant avian mortality.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Publications for WNVM Antibody (NB100-56743)(11)

We have publications tested in 5 confirmed species: Human, Mouse, Hamster, Monkey, Virus.

We have publications tested in 1 application: WB.

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Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using NB100-56743 Applications Species
Sasaki M, Anindita PD, Phongphaew W et al. Development of a rapid and quantitative method for the analysis of viral entry and release using a NanoLuc luciferase complementation assay. Virus Res. 2017-10-23 [PMID: 29074234] (WB, Human) WB Human
Boylan BT, Moreira FR, Carlson TW, Bernard KA. Mosquito cell-derived West Nile virus replicon particles mimic arbovirus inoculum and have reduced spread in mice. PLoS Negl Trop Dis. 2017-02-01 [PMID: 28187142] (WB, Mouse) WB Mouse
Zhang R, Miner JJ, Gorman MJ et al. A CRISPR screen defines a signal peptide processing pathway required by flaviviruses Nature. 2016-07-07 [PMID: 27383988] (WB, Hamster, Human, Monkey) WB Hamster, Human, Monkey
Merino-Ramos T, Blazquez AB, Escribano-Romero E et al. Protection of a Single Dose West Nile Virus Recombinant Subviral Particle Vaccine against Lineage 1 or 2 Strains and Analysis of the Cross-Reactivity with Usutu Virus. PLoS ONE. 2014-09-18 [PMID: 25229345] (WB, Virus)

WNVM antibody used for WB analysis of WNV proteins released to the culture medium from HeLa or 293T cells transfected with plasmid pcDNA-WNV, and a control lane containing purified WNV from WNV-infected Vero cells was included in th assay.
WB Virus
Martin-Acebes MA, Merino-Ramos T, Blazquez AB et al. The Composition of West Nile virus Lipid Envelope Unveils a Role of Sphingolipid Metabolism on Flavivirus Biogenesis. J Virol. 2014-08-13 [PMID: 25122799] (WB, Virus)

WNVM antibody used for WB on lysates of HeLa or HeLa3-WNV cells. FIG 2E shows two bands - one band compatible with prM protein and the other band compatible with mature M protein (about 20 and 6 kDa, respectively).
WB Virus
Dowd KA, Mukherjee S, Kuhn RJ, Pierson TC. Combined effects of the structural heterogeneity and dynamics of flaviviruses on antibody recognition. J Virol. 2014-07-30 [PMID: 25078693] (WB, Virus)

For the evaluation of the efficiency of prM cleavage in WNV RVP preparations, WNVM Antibody used for WB at 1:250 dilution on aliquots of WNV-prM+ and WNV-furin RVPs that were partially purified by centrifugation through a 20% sucrose cushion followed by lysis in buffer containing 1% Triton, 100 mM Tris, 2 M NaCl, and 100 mM EDTA.
WB Virus
Makino Y, Suzuki T, Hasebe R et al. Establishment of tracking system for West Nile virus entry and evidence of microtubule involvement in particle transport. J Virol Methods 2014-01-01 [PMID: 24140187] (WB, Human) WB Human
Lin TY, Dowd KA, Manhart CJ et al. A novel approach for the rapid mutagenesis and directed evolution of the structural genes of west nile virus. J Virol. 2012-04-01 [PMID: 22258236] (WB)

WB: Wild type and mutant (N15A) West Nile Virus harvested from infected BHk-21 cells, Fig 4F.
Tan TT, Bhuvanakantham R, Li J et al. Tyrosine 78 of premembrane protein is essential for assembly of West Nile virus. J Gen Virol. 2009-05-01 [PMID: 19264649] (WB)

BHk-21 cells mock-infected or infected with WNV, or co-transfected with WNV M and E plasmids were used with the IMG-5099A WNV M pAb in the following techniques and figures: 1. IF (Figs 2, 6) 2. WB (Figs 4a, 4b, 8a, 8c (iii)
Nelson S, Jost CA, Xu Q et al. Maturation of West Nile virus modulates sensitivity to antibody-mediated neutralization. PLoS Pathog. 2008-05-09 [PMID: 18464894]
Show All 11 Publications.

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